Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

The Effects of the Arabinoxylane and the Polysaccharide Peptide (PSP) on the Antiallergy, Anticancer

Arabinoxylane과 PSP의 항암 효과 및 알레르기 억제 효과 검증

  • Published : 2004.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

The objective of the current study was to determine the effects of arabinoxylane and polysaccharide peptide (PSP) on the immune cell functions. Both arabinoxylane and PSP increased plaque forming cell (10-15%) and rosette forming cell (10-30%) formation. Stimulation of macrophage with PSP (18%) and arabinoxylane (22%) resulted in increased phagocytic effects. Both arabinoxylane and PSP induced the tumor suppressive effect in mice injected sarcoma-180 cell intracutaneously. When the mice injected intraperitoneal cavity with sarcoma 180 cells, survival ratio was increased in the mice fed on arabinoxylane and PSP. The ratio of PCA was slightly decreased in the mice fed on PSP, especially fed on arabinoxylane than in the control mice. The concentrations of blood histamine were slightly decreased in the mice fed on arabinoxylane and PSP. These results suggest that the capacity of arabinoxylane and PSP seems to act as a potent immunomodulator causing augmentation of immune cell activity, and with the absence of notable side-effects, arabinoxylane and PSP could be used as a biological response modifier having possible therapeutic effects against immunological disorders.

본 연구에 사용한 아라비녹실란화합물은 미강에서 추출한 헤미셀룰로즈에 표고버섯균사체 배양물에서 분리한 효소를 작용시켜 생성된 식물성다당류이며, PSP(polysaccharide peptide)는 담자균류의 일종인 Coliolus versicocor Sov-1의 균사체에서 추출 정제하여 얻은 단백다당체 성분이다. 이들에 대한 면역세포 활성화 효과, 항암 효과 및 알레르기 억제효과 등에 관한 실험 결과는 다음과 같다. 비장세포의 B cell과 T cell에 arabinoxylane과 PSP의 항체생성과 세포활성능을 조사한 결과 PFC형 성은 10 ∼ 15%, RFC검출은 10 ∼ 30% 증가하였다 복강침출세포로부터 대식세포의 탐식능 효과는 대조군에 비하여 PSP는 약 18%, arabinoxylane은 22% 정도 증가하였다. S-180 고형암에 의한 항암실험에서 arabinoxylane은 66.2%(p<0.01), PSP는 49.0%(p<0.05)의 억제 효과를 확인할 수 있었다. 복수암에 의한 수명 연장효과는 PSP, arabinoxylane이 대조군에 비해 다소의 수명연장효과가 나타났으며, PSP가 아라비녹실란에 비해 약간의 높은 수명연장효과가 나타났다. 항알레르기 효과를 보기 위한 PCA 역가를 측정한 결과는 대조군이 80인데 비해 PSP군은 40, arabinoxylane군은 20으로 대조군에 비하여 다소 경감되었으며, arabinoxylane군이 다소 우수한 결과를 보였다. 혈중 histamine 함량도 PCA와 유사한 결과로 arabinoxylane군과 PSP군이 히스타민 함량을 다소 감소시키는 경향을 보였다.

Keywords

References

  1. Hirokazu O. 1994. Current topics in allergy. J Trad Sino-Japanese Med 15: 11-15.
  2. Dibner M. 1995. Biotechnology Guide USA. Institute for Biotechnology Information.
  3. Masatoshi K. 1994. Kampo-therapy of child asthma. J Trad Sino-Japanese Med 15: 16-22.
  4. Ikuo K, Seizaburo K, Teruo Y, Masahiko M. 1981. Antitumor activity of lactobacillus casei in mice. Gann 72: 517-523.
  5. Ghoneum M. 1999. NK immunorestoration of cancer patient by MGN-3, a modified arabinoxylan rice bran (study of 32 patients followed for up to 4 years). American Academy of Anti-Aging Med 10: 11-10.
  6. William DG. 1998. MGN-3: Quite possibly the most powerful immune booster known to man. Alternatives for the Health Conscious Individual 7: 113-120.
  7. Ghoneum M. 1998. Enhancement of human natural killer cell activity by modified arabinoxylane from rice bran (MGN-3). Int J Immunotherapy XIV: 89-99.
  8. Yang MM, Chen Z, Kwok JS. 1992. The anti-tumor effect of a small polypeptide from Coriolus versicolor. American J Chinese Med XX: 221-232.
  9. Qian ZM, Xu MF, Tang PL. 1997. Polysaccharide peptide (PSP) restores immunosuppression induced by cyclophosphamide in rats. American J Chines Med XXV: 27-35.
  10. Takino BN. 1998. A review of research on the proteinbound polysaccharide (Polysaccharopeptide, PSP) from the mushroom Coriolus versicolor (Basidiomycetes: Polyporaceae). Gen Pharmac 30: 1-4. https://doi.org/10.1016/S0306-3623(97)00076-1
  11. Jeong YR, Ha MH, Kim SH, Jo SK, Byun MW, Cho HW, Seo K, Yee ST. 2000. Immunosuppressive effects of herbal plant extracts on alloantigen reactive cell prolliferation and cytotoxicity. J Korean Soc Food Sci Nutr 29: 1133-1138.
  12. Cunningham AJ, Szemberg A. 1968. Further improvements in the plaque technique for detecting single antibody-forming cell. Immunol 14: 599-606.
  13. Garvey JS. 1980. Method in immunology. W.A. Benjamin Inc., New York. p 449-450.
  14. Suga T, Shiio T, Maeda Y, Chihara G. 1992. Antitumor activity of lentinan syngenetic and autoch-thonous hosts and its suppressive effect on 3-methyl cholanthrent-induced carcinogenesis. Cancer Res 52: 154-162.
  15. Maeda Y, Chihara G. 1993. The effect of neonatal thymectomy on the antitumor activity of lentinan, carboxylmethyl pachymaran and zymosan and their effects on various immune responses. Int J Cancer 11: 153-167. https://doi.org/10.1002/ijc.2910110118
  16. Kind LS, Macdo SO. 1973. Heterologous adoptive cutaneous anaphylaxis: A method for detecting reaginic antibody formation by cells of the mouse. J Immunol 111: 638-640.
  17. Shore PA, Burkhalter A, Cohn VH. 1992. A method for the fluorometric assay of histamine in tissues. J Pharmacol Exp Ther 127: 182-186.
  18. Kazuki S, Yuji O, Takashi K. 1986. Growth-promoting effects of an extract of a thermophilic BLUE-green alga, Synechococcus elongatus var. on human cell lines. Agric Biol Chem 50: 2225-2230. https://doi.org/10.1271/bbb1961.50.2225
  19. Chihara G, Hamuro H, Maeda Y, Arai Y, Fukuoka K. 1970. Fractionation and purification of the polysaccharides with marked antitumor activity, especially lentinan from Lentinus edodes. Cancer Res 30: 2776-2781.
  20. Minowata T, Ohnuma T, Moore GE. 1972. Brief communication: Rosette forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Nat Cancer Inst 49: 891-899.
  21. Wybran J, Carr MC, Fudenberg HH. 1972. The human rosette forming cell as a marker of thymus-derived cell. J Clin Invest 51: 2537. https://doi.org/10.1172/JCI107069
  22. Fumie K, Kiwamu O. 1992. Nutritional treatment to hepato cellular carcinoam. Japanese J Nutr Assessment 9: 38-42.
  23. Tsukagoshi S, Ohashi K. 1974. Protein-bound polysaccharide preparation, PS-K, effective against mouse sarcoma-180 and ascites hepatoma AH-13 by oral use. Gann 65: 557-558.
  24. Chihara G, Hamuro J, Maeda Y, Arai Y, Fukuoka F. 1970. Anti-tumour polysaccharide derived chemically from natural glucan (pachyman). Nature (Lond) 225: 943-950. https://doi.org/10.1038/225943a0
  25. Chihara G, Maeda Y, Hamuro J, Sasaki T, Fukuoka F. 1969. Inhibition of mouse sarcoma 180 by polysaccharides from Lentinus edoses (Berk.) Sing. Nature (Lond) 222: 687-692. https://doi.org/10.1038/222687a0
  26. Hamuro J, Hadding U, Suermann B. 1978. Solid phase activation of alternative pathway of complement by ${\beta}-1,3-glucan$ and its possible role for tumor regressing activity. J Immunol 34: 695-705.
  27. Hirokazu K, Ryosuke K, Toshimi S, Takash M, Toshihito H, Takwi N. 1988. Cytotoxic steroids from the mushroom Agaricus blazei. Phytochemistry 27: 2777-2782. https://doi.org/10.1016/0031-9422(88)80662-9
  28. Yiu TC, Ushio S. 1989. Screening of antiallergic effect in traditional medicinal drugs and active constituents of Auranyii fructus Immaturus. Shoyakugaku Zasshi 43: 314-318.
  29. Kazuhiro N, Shuichi T, Yukihiro S, Yukio S, Yasuyuki Y. 1992. Histamine release inhibition of bisbenzylisoquinoline alkaloids. Planta Med 58: 505-508. https://doi.org/10.1055/s-2006-961536
  30. Toyokazu K, Maxim, Masako K, Mitsuko P, Toshihiro I. 1989. Rulian bian and Qiangmin xie, studies on the antianaphylactic components of Asarum forbesi. J Immunol 43: 230-235.

Cited by

  1. Growth Inhibitory and Quinone Reductase Induction Activities of Salicornia herbacea L. Fractions on Human Cancer Cell Lines in vitro vol.37, pp.2, 2008, https://doi.org/10.3746/jkfn.2008.37.2.148
  2. 미역귀 분획물의 항균 · 암세포 성장저지 효과 vol.34, pp.6, 2005, https://doi.org/10.3746/jkfn.2005.34.6.765
  3. 약초 음료의 항균효과, 항암효과 및 알레르기 억제효과 검증 vol.34, pp.7, 2005, https://doi.org/10.3746/jkfn.2005.34.7.953
  4. 쌀겨 추출물의 항암효과(in vitro) 및 항알레르기 효과 vol.35, pp.10, 2004, https://doi.org/10.3746/jkfn.2006.35.10.1297
  5. 황기에 표고버섯 균사체를 배양한 추출물이 항암효과 및 알레르기 억제효과에 미치는 영향 vol.36, pp.1, 2007, https://doi.org/10.3746/jkfn.2007.36.1.008
  6. 인동초로 배양한 표고버섯 균사체 추출물의 항암 및 알레르기 억제효과 검증 vol.36, pp.4, 2004, https://doi.org/10.3746/jkfn.2007.36.4.424
  7. Baicalein의 BALB/c Mice에서의 접촉성 피부알레르기 예방효과 vol.38, pp.2, 2004, https://doi.org/10.3746/jkfn.2009.38.2.160
  8. 오징어내장 분획물의 in vitro에서의 암세포 성장억제 및 quinone reductase유도 활성 증가 효과 vol.19, pp.9, 2004, https://doi.org/10.5352/jls.2009.19.9.1251
  9. 군소내장 분획물의 in vitro에서의 암세포 성장억제 및 quinone reductase 유도 활성 증가 효과 vol.20, pp.6, 2010, https://doi.org/10.5352/jls.2010.20.6.877
  10. Changes in 2,6-dimethoxy-1,4-benzoquinone and Water Extractable Arabinoxylan Content of Wheat Germ Extract by Enzyme Treatment vol.23, pp.1, 2004, https://doi.org/10.13050/foodengprog.2019.23.1.22