• Journal of Microbiology
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• 제45권3호
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• pp.272-274
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• 2007

During May to July 2004, three strains of Providencia spp. with multidrug-resistance (MDR) were isolated from urinary specimen of three patients hospitalized with a same hospital room. By PCR analysis, all three strains have been found to carry both VIM-2 type $metallo-{\beta}-lactamase$ gene and PER-1 type extended spectrum ${\beta}-lactamase$ gene. One out of three strains carried additional resistance gene, armA, 16S rRNA methylase gene responsible for high level resistance to aminoglycosides. To our knowledge, this is the first report on the identification of Providencia spp. simultaneously carrying $bla_{VIM-2},\;bla_{PER-1}$, and armA genes.

• Journal of Yeungnam Medical Science
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• 제40권3호
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• pp.311-314
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• 2023

Thallium poisoning is usually accidental. We present a case of a 51-year-old woman who was evaluated in June 2018 for myalgia, vertigo, asthenia, and abdominal pain. Physical examination revealed temporal-spatial disorientation, jaundice, and asterixis. The laboratory reported the following: bilirubin, 10.3 mg/dL; aspartate transaminase, 78 U/L; alanine transaminase, 194 U/L; albumin, 2.3 g/dL; prothrombin time, 40%; and platelet count, 60,000/mm³. Serology performed for hepatitis A, B, and C; Epstein-Barr virus; cytomegalovirus; and human immunodeficiency virus was negative, and a collagenogram was negative. Physical reevaluation revealed alopecia on the scalp, armpits, and eyebrows; macules on the face; plantar hyperkeratosis; and ulcers on the lower limbs. Tests for lead, arsenic, copper, and mercury were carried out, which were normal; however, elevated urinary thallium (540 ㎍/g; range, 0.4-10 ㎍/g) was observed. The patient was treated with D-penicillamine 1,000 mg/day and recovered her urinary thallium levels were within normal range at annual follow-up. Thallium poisoning is extremely rare and can be fatal in small doses. An adequate clinical approach can facilitate early diagnosis.

• 한국응용곤충학회지
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• 제53권1호
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• pp.15-26
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• 2014

다양한 먹이 조건에서 생활하는 아메리카동애등에(Hermetia illucens)는 장내 세균의 의존성을 가질 수 있다. 이 가설을 증명하기 위해 본 연구는 종령 유충의 소화관에 존재하는 세균을 분리, 동정하고 효소활성 및 항균 능력을 분석하였다. 종령 유충의 소화관은 몸 체장에 약 7 배의 길이를 나타냈다. 한 개체의 소화관 내 존재하는 세균 수는 $5.0{\times}10^6$ cfu로 98% 이상이 후장에 존재했다. 소화관에는 3 종류의 상이한 세균이 존재했고, 미생물 동정 장치는 이들이 각각 Morganella morganii, Providencia rettgeri 및 Bacillus halodurans로 동정하였다. 이들 소화관 세균을 16S rDNA 서열을 분석한 결과 이 외에 Proteus mirabilis, Providencia alcalifaciens, Providencia sp.를 검출하였다. 이들 장내세균은 항생제 내성을 보였고, 타 미생물의 성장을 억제하였다. 또한 섬유소, 지질, 단백질 및 탄수화물의 분해 능력을 보유하였다. 본 연구 결과들은 아메리카동애등에 소화관에 유용성이 높은 세균을 보유하고 있다고 제시하였다.

• 생명과학회지
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• 제11권6호
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• pp.530-536
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• 2001

원유분해능이 강력한 해양균주를 얻고자 유류오염 지역 으로부터 crude oil을 탄소원으로 이용하는 십수종을 분리하였다. 분리된 균주중 원유분해 및 성장속도면에서 우수한 균주를 선별하여 형태학적, 생화학적 및 생리학적 특성을 조사한후 Providencia rettgeri 4A3로 동정하였다. 4A3균주의 원유분해를 위한 최적 배양조건은 배양온도 26$^{\circ}C$로 비교적 낮은 온도였으며, 초기 pH는 7.0이었다. 또한, 이 균주는 2.0% 염분농도에서 최대의 성장을 보여주어 해양 유래의균주임을 확인하였다. 4A3균주의 유화활성은 26$^{\circ}C$, pH 7.0의 배양조건으로 배양 3일째에 가장 높게 나타났으며, 유류분해의 경시적인 변화패턴을 관찰하였다. 분리균주는 약 7.0kb의 크기를 나타낸 미지의 플라스미드 1개를 보유하고 있었다.

• Current Research on Agriculture and Life Sciences
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• 제30권2호
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• pp.105-109
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• 2012

Blacken spoilage is one of the common problems during the cold storage of dried persimmon in Korea. We collected the spoiled dried persimmon in the refrigerator and classified them to 4 types depending on their appearances. Furthermore we isolated blacken spoilage inducing bacteria from type D of dried persimmons. Among the isolates we identified the seven blacken spoilage inducing bacteria. They are Aeromonas hydrophila DP1, Cedecea davisae DP2, Ewingella americana DP3, Flavimonas oryzihabitans DP4, Providencia rettgeri DP5, Providencia rustigianii DP6 and Serratia plymuthica DP7. Strains were identified based on their morphological, cultural and physiological properties. We also found that Ewingella americana DP3, Flavimonas oryzihabitans DP4 were the major blacken spoilage inducing bacteria during dried persimmon storage.

• 한국융합학회논문지
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• 제8권1호
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• pp.51-59
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• 2017

본 연구는 종합병원 내 응급실에 설치된 CT 장비와 일반촬영장비에 대한 세균 오염도 검사를 실시하여 보건학적 융복합 감염관리에 대한 기초자료를 마련하고자 하였다. 연구는 2015년 12월 1일부터 12월 31일까지 수도권 3곳과 전라도 2곳, 충청도 2곳 등 총 7곳의 의료기관을 대상으로하였다. 영상의학과 응급실 내 CT장비의 검출된 표면 오염 균주는 Micrococcus species(4,5%), Stenotrophomonas maltophilia(9%), Enteococcus faecium(4.5%), Providencia stuartii(4.5), Gram negative bacilli(4.5%), 일반촬영장비에서 검출된 표면 오염 균주는 Providencia stuartii(11%), Klebsiella pneumonia(3.5%), Stenotrophomonas maltophilia(11%), Pantoea species(11%), Acinetobacter baumannii(3.5%), Micrococcus species(3.5%), Escherichia coli(3.5%), Enterobacter species(3.5%), Gram negative bacilli(11%) 로 병원 감염의 원인균으로 알려진 균주는 없었고, 특이성을 가진 균주 역시 없었지만 가장 많이 검출된 구역이 모두 환자와 밀접한 관련을 갖는 곳이므로 방사선사는 검사 전후 알코올 등으로 깨끗이 닦아내야 할 것이다.

• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.968-978
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• 2007

A pathogenic nematode, Butlerius sp., was isolated from Oriental beetle, Blitopertha orientalis. The infective juveniles exhibited dose-as well as time-dependent entomopathogenicity on the larvae of B. orientalis. Two bacterial species, Providencia vermicola (KACC 91278) and Flavobacterium sp. (KACC 91279), were isolated from the infective juveniles and identified. P. vermicola outnumbered Flavobacterium sp. in the nematode host, in which the colony density of P. vermicola was found to be 21 times higher than that of Flavobacterium sp. However, when the two bacterial species were cocultured in culture media without the nematode host, they showed similar growth rates. Both bacteria induced significant entomopathogenicity against Spodoptera exigua larvae infesting economically important vegetable crops, where P. vermicola was more potent than Flavobacterium sp.

• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.234-241
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• 2017

Potato peels (PP) contain several bioactive compounds. These compounds are known to provide human health benefits, including antioxidant and antimicrobial properties. In addition, these compounds could have effects on human enteric viruses that have not yet been reported. The objective of the present study was to evaluate the phenolic composition, antioxidant properties in the acidified ethanol extract (AEE) and water extract of PP, and the antiviral effects on the inhibition of Av-05 and MS2 bacteriophages, which were used as human enteric viral surrogates. The AEE showed the highest phenolic content and antioxidant activity. Chlorogenic and caffeic acids were the major phenolic acids. In vitro analysis indicated that PP had a strong antioxidant activity. A 3 h incubation with AEE at a concentration of 5 mg/ml was needed to reduce the PFU/ml (plaque-forming unit per unit volume) of Av-05 and MS2 by 2.8 and $3.9log_{10}$, respectively, in a dose-dependent manner. Our data suggest that PP has potential to be a source of natural antioxidants against enteric viruses.

• 한국환경보건학회지
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• 제19권2호
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• pp.34-39
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• 1993

One hundred and eighty-seven water samples were collected from 23 of spring water, 2 of ground water, 1 of tap water in Pusan area and 3 of natural mineral waters. Total coliform group, fecal coliform, viable cell count and microflora were investigated to evaluate water quality of drinking water. The results were as follows: range and geometric mean value of total coliform and fecal coliform MPN's of spring water were 0~1,500/100 ml, 85/100 ml and 0~460/100 ml, 24/100 ml but coliform group was not detected in the samples of tap water and natural mineral water. Viable cell count of spring water, ground water and tap water were lower as 100 cell than the criteria for drinking water but that of natural mineral water was higher as 6.5X 10$^2$~7.4X 10$^3$ /ml. Predominant speces among the 219 strains isolated from the samples were 19.6% Aeromonas spp., 19.2% Enterobacteriaceae, 16% Acinetobacter spp. Especially, spring water and vessels were contaminated by Hafnia spp. and Providencia Spp, inhabitant of the oral cavity.

• Computers and Concrete
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• 제12권4호
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• pp.393-411
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• 2013

In the analysis and design of reinforced concrete frames beam-column joints are sometimes assumed as rigid. This simplifying assumption can be unsafe because it is likely to affect the distributions of internal forces and moments, reduce drift and increase the overall load-carrying capacity of the frame. This study is concerned with the relevance of shear deformation of beam-column joints, in particular of exterior ones, on the quasi-static behavior of regular reinforced concrete sway frames. The included parametric studies of a simple sub-frame model reveal that the quasi-static monotonic behavior of unbraced regular reinforced concrete frames is prone to be significantly affected by the deformation of beam-column joints.