Browse > Article

Multidrug-Resistant Providencia Isolates Carrying $bla_{PER-1},\;bla_{VIM-2}$, and armA  

Lee, Hee-Woo (Department of Microbiology, Kyungpook National University, School of Medicine)
Kang, Hee-Young (Department of Microbiology, Kyungpook National University, School of Medicine)
Shin, Kyeong-Seob (Department of Laboratory Medicine, College of Medicine Chungbuk National University)
Kim, Jung-Min (Department of Microbiology, Kyungpook National University, School of Medicine)
Publication Information
Journal of Microbiology / v.45, no.3, 2007 , pp. 272-274 More about this Journal
Abstract
During May to July 2004, three strains of Providencia spp. with multidrug-resistance (MDR) were isolated from urinary specimen of three patients hospitalized with a same hospital room. By PCR analysis, all three strains have been found to carry both VIM-2 type $metallo-{\beta}-lactamase$ gene and PER-1 type extended spectrum ${\beta}-lactamase$ gene. One out of three strains carried additional resistance gene, armA, 16S rRNA methylase gene responsible for high level resistance to aminoglycosides. To our knowledge, this is the first report on the identification of Providencia spp. simultaneously carrying $bla_{VIM-2},\;bla_{PER-1}$, and armA genes.
Keywords
Providencia; $metallo-{\beta}-lactamase$; extended-spectrum ${\beta}-lactamase$; 16S rRNA methylase;
Citations & Related Records

Times Cited By Web Of Science : 8  (Related Records In Web of Science)
Times Cited By SCOPUS : 6
연도 인용수 순위
1 Lee, K., Y.S. Lim, D. Yong, J.H. Yum, and Y. Chong. 2003. Evaluation of the Hodge test and imipenem-EDTA double-disk synergy test for differentiating metallo-${\beta}$-lactamase-producing isolates of Pseudomonas spp. and Acinetobacter spp. J. Clin. Microbiol. 41, 4623-4629   DOI
2 Poirel, L., T. Lambert, S. Turkoglu, E. Ronco, J. Gaillard, and P. Nordmann. 2001. Characterization of class 1 integrons from Pseudomonas aeruginosa that contain the $bla_{VIM-2}$ carbapenem- hydrolyzing ${\beta}$-lactamase gene and of two novel aminoglycoside resistance gene cassettes. Antimicrob. Agents Chemother. 45, 546-552   DOI   ScienceOn
3 Yong, D., J.H. Shin, S. Kim, Y. Lim, J.H. Yum, K. Lee, Y. Chong, and A. Bauernfeind. 2003. High Prevalence of PER-1 Extendedspectrum ${\beta}$-lactamase-producing Acinetobacter spp. in Korea. Antimicrob. Agents Chemother. 5, 1749-1751
4 Yum, J.H., K. Yi, H. Lee, D. Yong, K. Lee, J.M. Kim, G.M. Rossolini, and Y. Chong. 2002a. Molecular characterization of metallo-${\beta}$-lactamase producing Acinetobacter baumannii and Acinetobacter genomospecies 3 from Korea: identification of two new integron carrying the blaVIM-2 gene cassettes. J. Antimicrob. Chemother. 49, 837-840   DOI   ScienceOn
5 Bahar, G., B. Erac, A. Mert, and Z. Gulay. 2004. PER-1 production in a urinary isolate of Providencia rettgeri. J. Chemother. 16, 343-346   DOI
6 Gautom, R.K. 1997. Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other Gram-negative organism in 1 day. J. Clin. Microbiol. 35, 2977-2980   PUBMED
7 Shin, K.S., K. Han, J. Lee, S.B. Hong, B.R. Bon, S.J. Youn, J. Kim, and H.S. Shin. 2005. Imipenem-resistant Achromobacter xylosoxidans carrying blaVIM-2-containing class 1 integron. Diagn. Microb. Infect. Dis. 53, 215-220   DOI   ScienceOn
8 National Committee for Clinical Laboratory Standards. 2003. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, approved standard M7-A5, (6th ed). National Committee for Clinical Laboratory Standards, Wayne, Pa., USA
9 Yum, J.H., D. Yong, K. Lee, H.S. Kim, and Y. Chong. 2002b. A new integron carrying VIM-2 metallo-${\beta}$-lactamase gene cassette in a Serratia marcescens isolate. Diagn. Microbiol. Infect. Dis. 42, 217-219   DOI   ScienceOn
10 Vahaboglu, H., R. Ozturk, G. Aygun, F. Coskunkan, F. Yaman, A. Kaygusuz, H. Leblebicioglu, I. Balik, K. Aydin, and M. Otkun. 1997. Widespread detection of PER-1-type extended-spectrum ${\beta}$-lactamases among nosocomial Acinetobacter and Pseudomonas aeruginosa isolates in Turkey: A nationwide multicenter study. Antimicrob. Agents Chemother. 41, 2265-2269   PUBMED
11 Lee, H., D. Yong, J.H. Yum, K.H. Roh, K. Lee, K. Yamane, Y. Arakawa, and Y. Chong. 2006. Dissemination of 16S rRNA methylase-mediated highly amikacin-resistant isolates of Klebsiella pneumoniae and Acinetobacter baumannii in Korea. Diag. Microbiol. Infect. Dis. 56, 305-312   DOI   ScienceOn
12 Jeong, S.H., K. Lee, Y. Chong, J.H. Yum, S.H. Lee, H.J. Choi, Y.M. Kim, K.H. Park, B.H. Han, S.W. Lee, and T.S. Jeong. 2003. Characterization of a new integron containing VIM-2, a metallo-${\beta}$-lactmase gene cassettes, in a clinical isolate of Enterobacter cloacae. J. Antimicrob. Chemother. 51, 397-400   DOI   ScienceOn
13 Nordmann, P., E. Ronco, T. Naas, C. Duport, Y. Michel-Briand, and R. Labia. 1993. Characterization of a novel extendedspectrum ${\beta}$-lactamase from Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 37, 962-969   DOI   PUBMED   ScienceOn
14 Poirel, L., T. Naas, D. Nicolas, L. Collet, S. Bellais, J.D. Cavallo, and P. Nordmann. 2000. Characterization of VIM-2, a carbapenem- hydrolyzing metallo-${\beta}$-lactamase and its plasmid- and integron-borne gene from a Pseudomonas aeruginosa clinical isolate in France. Antimicrob. Agents Chemother. 44, 891-897   DOI
15 Vahaboglü, H., S. Dodanli, C. Eroglu, R. Ozturk, G. Soyletir, I. Yildirim, and V. Avkan. 1996. Characterization of multipleantibiotic- resistant Salmonella typhimurium strains: molecular epidemiology of PER-1-producing isolates and evidence for nosocomial plasmid exchange by a clone. J. Clin. Microbiol. 34, 2942-2946   PUBMED
16 Galimand, M., P. Coruvalin, and T. Lambert. 2003. Plasmid-mediated high-level resistance to aminoglycosides in Enterobacteriaceae due to 16S rRNA methylation. Antimicrob. Agents Chemother. 47, 2565-2571   DOI
17 Mingeot-Leclercq, M-P., Y. Glupczynski, and P. Tulkens. 1999. Aminoglycosides: activity and resistance. Antimicrob. Agents Chemother. 43, 727-737
18 Lee, K., J.B. Lim, J.H. Yum, D. Yong, Y. Chong, J.M. Kim, and D.M. Livermore. 2002. $bla_{VIM-2}$ cassette-containing novel integrons in metallo-${\beta}$-lactamase-producing Pseudomonas aeruginosa and Pseudomonas putida isolates disseminated in a Korean hospital. Antimicrob. Agents Chemother. 46, 1053-1058   DOI
19 Poirel, L., L. Cavan, H. Vahaboglu, and P. Nordmann. 2005. Genetic environment and expression of the extended-spectrum ${\beta}$-lactamase $bla_{PER-1}$ gene in gram-negative bacteria. Antimicrob. Agents Chemother. 49, 1708-1713   DOI   ScienceOn
20 Docquier, J.D., F. Luzzaro, G. Amicosante, A. Toniolo, and G.M. Rossolini. 2001. Multidrug-resistant Pseudomonas aeruginosa producing PER-1 extended-spectrum serine-beta-lactamase and VIM-2 metallo-beta-lactamase. Emerg. Infect. Dis. 7, 910-911   DOI   ScienceOn
21 Peter, Z. 1992. Opportunistic Enterobacteriaceae, p. 551-552. In W.K. Joklik, H.P. Willett, D. Bernard Amos, and C.M. Wilfert (ed.). Zinsser Microbiology, (20th ed.) Appleton & Lange, Norwalk, Conneticut/San Maeto, CA, USA
22 Yan, J.J., J.J. Wu, W.C. Ko, S.H. Tsai, C.L. Chuang, H.M. Wu, Y.J. Lu, and J.D. Li. 2004. Plasmid-mediated 16S rRNA methylases conferring high-level aminoglycoside resistance in Escherichia coli and Klebsiella pneumoniae isolates from two Taiwanese hospitals. J. Antimicrob. Chemother. 54, 1007-1012   DOI   ScienceOn