• BMB Reports
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• 제44권11호
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• pp.705-712
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• 2011

Advances in the fields of proteomics and genomics have necessitated the development of high-throughput screening methods (HTS) for the systematic transformation of large amounts of biological/chemical data into an organized database of knowledge. Microfluidic systems are ideally suited for high-throughput biochemical experimentation since they offer high analytical throughput, consume minute quantities of expensive biological reagents, exhibit superior sensitivity and functionality compared to traditional micro-array techniques and can be integrated within complex experimental work flows. A range of basic biochemical and molecular biological operations have been transferred to chip-based microfluidic formats over the last decade, including gene sequencing, emulsion PCR, immunoassays, electrophoresis, cell-based assays, expression cloning and macromolecule blotting. In this review, we highlight some of the recent advances in the application of microfluidics to biochemistry and molecular biology.

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.783-788
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• 2004

Marker proteins of genetically-modified organisms (GMO) and their antibodies were prepared and characterized as major components of an analytical system. We selected two GMO markers, neomycin phosphotransferase II and 5- enolpyruvylshikimate-3-phosphate synthase, and produced them from E. coli employing genetic recombination technology. After purification, their structural conformation and binding affinities to the respective antibodies were characterized. The results showed that the recombinant proteins were identical with commercially obtained reference proteins. We further used them as immunogens to raise polyclonal antibodies capable of discriminating GMO containing protein from non-GMO. Well-characterized marker proteins and antibodies will be valuable as immunoreagents in constructing analytical systems such as biosensors and biochips to measure quantities of GMO.

• 센서학회지
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• 제14권3호
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• pp.156-159
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• 2005

Liposomes are microscopic spherical vesicles that form when lipids are hydrated and have been widely used for biochemical assay, drug delivery and molecular imaging. In particular, they are well known for artificial cell membranes to study cellular functions such as cell fusions and membrane proteins. Here, we firstly report the detection of liposomes by the highly sensitive microfabricated piezoresistive cantilever sensor chip and the phosphatidylserine recognition protein C2A which is chemically immobilized on the sensor surface. The signal created from the bending motion of piezoresistive cantilever after the liposome attachment has been monitored in real time.

• 한방안이비인후피부과학회지
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• 제21권1호
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• pp.55-69
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• 2008

Objective : This study was designed to investigated effects of Gleditsia spina (GS) on human derived melanoma cells Methods : The genetic profile for the effect of medicine on human derived melanoma cells of SK-MEL-2, was measured by using microarray technique, and the functional analysis on these genes was conducted. The network of total protein interactions was measured by using cytoscape program. Results : Total 253 genes were up-regulated and 439 genes down-regulated in cells treated with GS. Genes induced or suppressed by GS were all mainly concerned with metabolic process, regulation of biological process and protein binding. Conclusion : Suggest the possibility of GS as anti-cancer drug and cosmetic agent, and also suggest that related mechanisms are involved in regulation of intra-cellular metabolism in melanoma cells.

• 한국산학기술학회논문지
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• 제10권6호
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• pp.1407-1413
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• 2009

본 연구에서는 쥐의 미성숙 난자의 체외 성숙과정에서 매우 특이적으로 발현되는 후보 단백질 변화를 동정하려는 목적으로 체외 성숙 과정에서 GV 단계의 미성숙 난자와 MIl 단계의 난자를 실험 시료로 사용하였다. 그리고 단백질 Chip은 선행 실험에서 가장 효과적인 CM10을 사용하여 SELDI -TOF MS 분석 장치를 이용하여 후보단백질을 동정하였다. MIl 단계의 미성숙 난자와 비교하여 GV 단계의 미성숙 난자에서 16개의 후보단백질이 높게 발현되었으며, 이때 발현된 후보 단백질 각각의 분자량은 8180(후보단백질 2개), 10226 (5개), 15767(5개), 16770(4개) 달톤(dalton)이였다. 또한 29개의 후보단백질은 MIl 단계의 미성숙 난자에서 높게 발현되었고 이들의 분자량은 각각 10832(3개)17743(8개)20122(3개)22131(3개) 24857(7개) 33507(5개) 달톤 이였다. 한편 전체 후보 단백질 45개의 분석을 Real time RT-PCR에서 수행하여 13개의 잠재적인 후보단백질을 확인 동정하였다.

• 기술사
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• 제28권5호
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• pp.12-19
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• 1995

한국산 구약 감자(elephant-foot=Amorphoph-alus Konjak K.Koch)의 이화학적 성분 조성을 조사하기 위하여 국산 생감자와 분말을 시료로 하여 외국산 정분과 같이 분석한 결과 생감자는 수분이 80.64%였고 고형분의 대부분은 당류, 단백질, 희분이고 섬유나 지방은 미량 존재하였다. 건조 분말 정제품인 정분은 당류 74.42-80.41%, 수분은 10% 전후였고 만난 함량은 한국산이 일본, 중국산보다 상당히 적었고 역시 가용성 유리당도 일본산이 높고 한국산이 낮았는데 구성당은 한국산은 Sucrose가 많은 대신 외국산은 Fructose, Glu-cose 함량이 높았다. 한국산 정분의 단백질 함량은 외국산보다 2배 많았고 이의 amino acid 조성은 Glutamic acid, Aspartic acid, Arginine 등이 총아미노산의 45%를 차지하고 Valine, Serine, Leucines, Glycine의 순서로 많았다. 정분의 무기물은 K가 많고 P>Na>Ca순으로 많게 함유하였고 chip의 정분 수율은 한국산이 61%, 중국산은 57.6%였으며 정분의 백도는 색도 측정치는 중국산이 약간 높았으나 육안으로는 별 차이가 없이 비슷하였다.

• Reproductive and Developmental Biology
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• 제32권2호
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• pp.71-79
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• 2008

Surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF MS) is one of the recently developed proteomic technologies which is based on capturing proteins and peptides by chemically modified surfaces and highly sensitive for the analysis of complex biological samples. In the present study, to gain insights into oocyte maturation and early embryo development, SELDI-TOF-MS was used to find the protein candidates that are specifically or prominently expressed in porcine oocytes at the in vitro matured metaphase II (MIIl) and germinal vesicle (GV) stages. By selected CM10 chip, 16 candidates were found to be up-regulated in GV stage oocytes compared with in MII stage oocytes, their molecular weights were 8,180 (2 candidates), 10,226 (5 candidates), 15,767 (5 candidates) and 16,770 (4 candidates) Da respectively. And the expression of 29 candidates were higher in MII than in GV stage oocytes, their molecular weight were 10,832 (3 candidates), 17,743 (8 candidates), 20,122 (3 candidates), 22,131 (3 candidates), 24,857 (7 candidates) and 33,507 (5 candidates) Da, respectively. The expression of selected 13 candidates (0.2 and 1.0 % error tolerances) were analyzed using real time RT-PCR. The proteins that differentially regulated during oocyte in vitro maturation in the pigs may be potential biomarkers of oocyte maturation and quality.

• 대한의용생체공학회:의공학회지
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• 제34권3호
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• pp.123-128
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• 2013

The 'Dielectrophoretic Tweezers(DEP Tweezers)' can be used as a facile, economical toolkit for quantitative measurement of chemical and biological binding forces related to many biological interactions within a microfluidic device. Our experimental setup can probe the interaction between a single receptor molecule and its specific ligand. Immunoglobulin G(IgG) functionalized on polystyrene microspheres has been used to detect individual surface linked Staphylococcus protein A(SpA) molecules and to characterize the strength of the noncovalent IgG-SpA bond. It was measured and compared with the existing measurements. Measured single binding force of between Goat, Rabbit IgG and SpA were $17{\pm}7pN$, $74{\pm}16pN$. This work can be used to investigate several different ligand-receptor interactions and antigen-antibody interactions.

• Asian-Australasian Journal of Animal Sciences
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• 제30권3호
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• pp.309-319
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• 2017

Objective: Holsteins are known as the world's highest-milk producing dairy cattle. The purpose of this study was to identify genetic regions strongly associated with milk traits (milk production, fat, and protein) using Korean Holstein data. Methods: This study was performed using single nucleotide polymorphism (SNP) chip data (Illumina BovineSNP50 Beadchip) of 911 Korean Holstein individuals. We inferred each genomic estimated breeding values based on best linear unbiased prediction (BLUP) and ridge regression using BLUPF90 and R. We then performed a genome-wide association study and identified genetic regions related to milk traits. Results: We identified 9, 6, and 17 significant genetic regions related to milk production, fat and protein, respectively. These genes are newly reported in the genetic association with milk traits of Holstein. Conclusion: This study complements a recent Holstein genome-wide association studies that identified other SNPs and genes as the most significant variants. These results will help to expand the knowledge of the polygenic nature of milk production in Holsteins.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제54권8호
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• pp.378-383
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• 2005

We report the development of miniature fluorescence detection systems that employ miniature prism, mirrors and low coat CCD camera to detect the fluorescence emitted from 40 fluorescently-labeled protein patterns without scanner. This kind of miniature fluorescence detection system can be used in point of care. We introduce two systems, one uses prism+mirror block and the other uses prism and two mirrors. A large NA microscope eyepiece and low cost CCD camera are used. We fabricated protein chip containing multi-pattern BSA labeled with Cy5, using MEMS technology and modified the surface chemically to clean and to immobilize proteins. The measurements show that the combination of prism and mirrors can homogenize elliptical excitation light over the sample with higher optical efficiency, and increase the separation between excitation and fluorescence light at the CCD to give higher signal intensity and higher signal to noise ratio. The measurements also show that protein concentrations ranging from 10 ng/ml to 1000 ng/ml can be assayed with very small error. We believe that the proposed fluorescence detection system can be refined to build a commercially valuable hand-held or miniature detection device.