Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Identification of Developmental Related Genes in the Lab Animal

실험동물 발생 유전자의 확인

  • 이재달 (혜전대학 애완동물관리과)
  • Published : 2009.06.30
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Abstract

The present study was conducted to gain insights into oocyte maturation and early embryo development, SELDI-TOF-MS was used to find the protein candidates that are specifically or prominently expressed in mouse oocytes at the in vitro matured metaphase II (MIl) and germinal vesicle (GV) stages. By using selected CM10 chip, found 16 candidates which were up-regulated in GV stage oocytes compared with in MIl stage oocytes, molecular weight are 8180 (2 candidates), 10226 (5 candidates), 15767 (5 candidates) and 16770 (4 candidates) Da respectively. And 29 candidates were higher in MIl than in GV stage oocytes, molecular weight are 10832 (3 candidates), 17744(8 candidates), 20122 (3 candidates), 22131 (3 candidates), 24857 (7 candidates) and 33507 (5 candidates) Da, respectively. All (45) candidate (0.2 and 1.0 % error tolerances) were performed real time RT-PCR analysis and further selected 13 more potential candidates.

본 연구에서는 쥐의 미성숙 난자의 체외 성숙과정에서 매우 특이적으로 발현되는 후보 단백질 변화를 동정하려는 목적으로 체외 성숙 과정에서 GV 단계의 미성숙 난자와 MIl 단계의 난자를 실험 시료로 사용하였다. 그리고 단백질 Chip은 선행 실험에서 가장 효과적인 CM10을 사용하여 SELDI -TOF MS 분석 장치를 이용하여 후보단백질을 동정하였다. MIl 단계의 미성숙 난자와 비교하여 GV 단계의 미성숙 난자에서 16개의 후보단백질이 높게 발현되었으며, 이때 발현된 후보 단백질 각각의 분자량은 8180(후보단백질 2개), 10226 (5개), 15767(5개), 16770(4개) 달톤(dalton)이였다. 또한 29개의 후보단백질은 MIl 단계의 미성숙 난자에서 높게 발현되었고 이들의 분자량은 각각 10832(3개)17743(8개)20122(3개)22131(3개) 24857(7개) 33507(5개) 달톤 이였다. 한편 전체 후보 단백질 45개의 분석을 Real time RT-PCR에서 수행하여 13개의 잠재적인 후보단백질을 확인 동정하였다.

Keywords

References

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