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Identification of Protein Candidates in Porcine Oocytes during In Vitro Maturation  

Lee, Jae-Dal (Department of Animal Science, Chungbuk National University)
Cui, Xiang-Shun (Department of Animal Science, Chungbuk National University)
Im, Gi-Sun (National Livestock Research Institute, RDA)
Seong, Hwan-Hoo (National Livestock Research Institute, RDA)
Kim, Nam-Hyung (Department of Animal Science, Chungbuk National University)
Publication Information
Reproductive and Developmental Biology / v.32, no.2, 2008 , pp. 71-79 More about this Journal
Abstract
Surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF MS) is one of the recently developed proteomic technologies which is based on capturing proteins and peptides by chemically modified surfaces and highly sensitive for the analysis of complex biological samples. In the present study, to gain insights into oocyte maturation and early embryo development, SELDI-TOF-MS was used to find the protein candidates that are specifically or prominently expressed in porcine oocytes at the in vitro matured metaphase II (MIIl) and germinal vesicle (GV) stages. By selected CM10 chip, 16 candidates were found to be up-regulated in GV stage oocytes compared with in MII stage oocytes, their molecular weights were 8,180 (2 candidates), 10,226 (5 candidates), 15,767 (5 candidates) and 16,770 (4 candidates) Da respectively. And the expression of 29 candidates were higher in MII than in GV stage oocytes, their molecular weight were 10,832 (3 candidates), 17,743 (8 candidates), 20,122 (3 candidates), 22,131 (3 candidates), 24,857 (7 candidates) and 33,507 (5 candidates) Da, respectively. The expression of selected 13 candidates (0.2 and 1.0 % error tolerances) were analyzed using real time RT-PCR. The proteins that differentially regulated during oocyte in vitro maturation in the pigs may be potential biomarkers of oocyte maturation and quality.
Keywords
Pig oocyte; In vitro maturation; SELDI-TOF; Protein candidate;
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