[KSCI] Korea Science Citation Index Service

Production of Recombinant Proteins as Immuno-Analytical Markers of Genetically-Modified Organisms (GMO)

Hwang, Ok-Hwa (Graduate School of Biotechnology, Korea University)
Park, Hyuk-Gu (Graduate School of Biotechnology, Korea University)
Paek, Eui-Hwan (BioDigit Laboratories Corp., Technology Incubation Center, Biotechnology Building, Korea University)
Paek, Se-Hwan (Graduate School of Biotechnology, Korea University, Department of Biotechnology, Korea University)
Park, Won-Mok (Graduate School of Biotechnology, Korea University)

Publication Information

Journal of Microbiology and Biotechnology / v.14, no.4, 2004 , pp. 783-788 More about this Journal

Abstract

Marker proteins of genetically-modified organisms (GMO) and their antibodies were prepared and characterized as major components of an analytical system. We selected two GMO markers, neomycin phosphotransferase II and 5- enolpyruvylshikimate-3-phosphate synthase, and produced them from E. coli employing genetic recombination technology. After purification, their structural conformation and binding affinities to the respective antibodies were characterized. The results showed that the recombinant proteins were identical with commercially obtained reference proteins. We further used them as immunogens to raise polyclonal antibodies capable of discriminating GMO containing protein from non-GMO. Well-characterized marker proteins and antibodies will be valuable as immunoreagents in constructing analytical systems such as biosensors and biochips to measure quantities of GMO.

Keywords

GMO markers; gene cloning; recombinant proteins; mass production; antibodies; protein chip;

Citations & Related Records

Times Cited By KSCI : 4 (Citation Analysis)
Times Cited By Web Of Science : 0 (Related Records In Web of Science)

Reference
Cited By KSCI

1	Detection of genetically modified organism in foods / [ Ahmed, F. E. ] / Trends Biotechnol. DOI ScienceOn
2	A rapid alkaline extraction procedure for screening recombinant plasmid DNA / [ Birnboim, H. C.;J. Doly ] / Nucl. Acids Res. DOI ScienceOn
3	A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding / [ Bradford, M. M. ] / Anal. Biochem. DOI ScienceOn
4	Thrombin specificity. Requirement for apolar amino acids adjacent to the thrombin cleavage site of polypeptide substrate / [ Chang, J. Y. ] / Eur. J. Biochem. DOI ScienceOn
5	Molecular dissection of symptom determinants of Cucumber mosaic virus / [ Choi, S. K. ] / Dept. of Agronomy major in plant protection, Korea University, Thesis for the degree of doctor of philosophy
6	Analyze of the lung fluke antigen used a isoelectric focusing and immunoblotting / [ Park, H. S. ] / Dept. of Medicine, Korea University, Thesis for the degree of doctor of philosophy
7	The QIAexpressionist / [ QIAGEN ] / A Handbook for High-Level Expression and Purification of 6xHis-Tagged Proteins
8	Scientific principles for ecologically based risk assessment of transgenic organisms / [ Regal, P. J. ] / Molec. Ecol. DOI
9	Enzyme linked immunsorbent assay for quantitation of Neomycin phosphotransferaseⅡin genetically modified cotton tissue extracts / [ Rogan, G. J.;J. E. Ream;S. A. Berberich;R. L. Fuchs ] / J. Agric. Food Chem. DOI
10	Immunodiagnostic methods for detection of 5-enolpyruvylshikimate-3-phosphate synthase in Roundup Ready soybean / [ Rogan, G. J.;Y. A. Dudin;T. C. Lee;K. M. Magin;J. D. Astwood;N. S. Bhakta;J. N. Leach;P. R. Sanders;R. L. Fuchs ] / Food Control DOI ScienceOn
11	International biosafety: A global imperative / [ Regal, P. J. ] / The Scientist
12	Validation of an immunoassay for detection and quantitation of a genetically modified soybean in food and food fractions using reference materials / [ Lipp, M.;E. Anklam ] / J. AOAC Int.
13	Genetically modified medicinal plants. Ⅱ. Transfer and expression of a marker kanamycin resistance gene in Atropa belladonna plants / [ Cucu, N.;G. N. Tenea;L. Gavrila ] / Roum. Biotechnol. Lett.
14	Purification and characterization of microbially expressed Neomycin phosphotransferaseⅡ (NPTⅡ) protein and its equivalence to the plant expressed protein / [ Fuchs, R. L.;R. A. Heeren;M. E. Gustafson;G. J. Rogan;E. E. Bartnicki;R. M. Leimgruber;R. F. Finn;A. Hershman;S. A. Berberich ] / J. Biotechnol. DOI ScienceOn
15	An immunoaffinity immobilized enzyme assay for Neomycin phosphotransferase Ⅱ in crude cell extracts / [ Henderson, L.;A. Gururaj Rao;J. Howard ] / Protein Express. Purif.
16	Production of new modified proteins in transgenic plants / [ Herbers, K.;U. Sonnewald ] / Curr. Opin. Biotechnol. DOI ScienceOn
17	Detection of Fusarium species by enzyme-linked immunosorbent assay using monoclonal antibody / [ Kwak, B. Y.;B. J. Kwon;C. H. Kweon;D. H. Shon ] / J. Microbiol. Biotechnol.
18	Development of rapid molecular detection marker for Colletotrichum spp. in leaf and fruit tissues of sweet persimmon / [ Lee, S.-P.;Y.-S. Lee ] / J. Microbiol. Biotechnol.
19	IUPAC collaborative trial study of a method to detect the presence of genetically modified organism in soybeans and maize / [ Lipp, M.;P. Brodmann;K. Pietsch;J. Pauwels;E. Anklam ] / J. AOAC Int.
20	Modified ELISA for the detection of Neomycin phosphotransferaseⅡin transformed plant species / [ McKenzie, M. J.;V. Mett;P. E. Jameson ] / Plant Cell Reports DOI ScienceOn
21	Safety assurance of foods derived by modern biotechnology in the United States / [ Maryanski, J. H. ] / Presentation at the BioJapan 96 Symposium
22	Expression and purification of delta sleep-inducing peptide in Escherichia coli / [ Oh, K. S.;D. K. Na;M. H. Kweon;H. C. Sung ] / J. Microbiol. Biotechnol.
23	Expression and purification of an ACE-inhibitory peptide multimer from synthetic DNA in Escherichia coli / [ Oh, K. S.;Y. S. Park;H. C. Sung ] / J. Microbiol. Biotechnol.
24	Genome organization of Odontoglossum ringspot virus / [ Ryu, K .H. ] / Dept. of Agronomy major in plant protection, Korea University, Thesis for the degree of doctor of philosophy
25	/ [ Sambrook, J.;E. F. Fritsch;T. Maniatis ] / Molecular Cloning: A Laboratory Manual
26	Expression and affinity purification of recombinant proteins from plants / [ Urvee, A. D.;G. Sur;S. Daunert;R. Babbitt;Q. Li ] / Protein Express. Purif. DOI ScienceOn