• International Journal of Industrial Entomology and Biomaterials
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• 제48권1호
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• pp.33-41
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• 2024

To investigate the effect of sawdust fermentation period and storage period after fermentation on development as food for P. brevitarsis larvae, individual and group breeding were conducted on berry sawdust and oak sawdust. In individual breeding, the growth period of P. brevitarsis larvae was reduced by 12 days from the 60-day fermentation of berry sawdust to the 40-day fermentation of berry sawdust, 30 days from the 90-day fermentation of oak sawdust, and the weight of the larvae was the heaviest. In group breeding, the time it takes for P. brevitarsis larvae to change from 1^st to 3^rd instar is about 30 days after hatching from 60-day fermentation of berry sawdust, while 90-day fermentation of oak sawdust took more than 60 days, so the growth speed was fast and the survival rate was good. The results of the farmhouse demonstration test were the same trend, and it was judged that it would be possible to produce P. brevitarsis larvae with berries fermented sawdust, and it would be advantageous in terms of economy. In addition, for both individual and group breeding, the growth period of P. brevitarsis larvae was longer as the storage period was longer, the weight of the larvae decreased, and the survival rate was no different. The development period of P. brevitarsis larvae was the longest in the storage period of 18 months for berries fermented sawdust, and the storage period of oak fermented sawdust was longer in the storage period of 12 months and 18 months. Therefore, considering the results of individual breeding and group breeding, the fermentation period was appropriate for 60 days for berries sawdust, and the storage period for berries sawdust was stable from 0 to 12 months, and 0 to 6 months for oak sawdust.

• 생명과학회지
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• 제32권11호
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• pp.890-898
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• 2022

본 연구에서는 유산균 발효가 큰느타리버섯 수확후배지를 식이한 흰점박이꽃무지 유충의 항산화 활성에 미치는 영향을 평가하기 위하여 흰점박이꽃무지 유충을 유산균으로 발효시키고, 흰점박이꽃무지 유충(PLE)과 발효 흰점박이꽃무지 유충(FPLE) 추출물의 항산화 활성을 비교하였다. 추출물 PLE와 FPLE의 총 폴리페놀 함량은 각각 65.02±1.32 mg GAEs/extract g과 93.33±0.98 mg GAEs/extract g이었고, 플라보노이드 함량은 각각 18.3±1.57 mg QEs/extract g과 17.69±0.95 mg QEs/extract g이었다. 추출물 PLE와 FPLE의 DPPH에 의한 라디칼소거 활성은 2-4 mg/ml의 농도에서는 두 시료 간의 유의적 차이를 나타나지 않았지만, 8mg/ml 이상의 농도에서는 추출물 PLE에 비해 FPLE의 DPPH 라디칼 소거능이 우수하였다. 추출물 PLE와 FPLE의 환원력도 추출물 PLE에 비해 FPLE이 우수하였으며 1.6 mg/ml 이상의 농도에서는 PLE에 비해 FPLE의 환원력이 2배 이상 높게 나타났다. 추출물 PLE와 FPLE의 ORAC 지수는 각각 74.34±0.37 uM TEs/extract g과 79.22±0.72 uM TEs/extract g으로 추추물 PLE에 비해 FPLE의 ORAC 지수가 높게 나타났고, RAW 264.7 세포에 대한 추출물의 세포독성은 나타나지 않았다. 이상의 결과를 종합하면, 큰느타리버섯 수확후 배지를 식이한 흰점박이꽃무지 유충은 유산균 발효에 의해 항산화물질인 총 폴리페놀 함량 및 항산화 활성이 증가된 것을 확인할 수 있었으며, 발효법은 흰점박이꽃무지 유충을 식의약품 소재로 개발하는 과정에 필요한 전처리 과정이라고 판단된다.

• 한국환경과학회지
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• 제31권11호
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• pp.989-992
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• 2022

This study was conducted to investigate the efficacy of larval stages of three species, namely, Tenebrio molitor, Protaetia brevitarsis seulensis, and Ptecticus tenebrifer larvae, in degrading poultry manure, specially, broiler and duck manure. The survival rates of larvae were also noted. For the experiment, T. molitor (n=300), P. brevitarsis seulensis (n=60), and P. tenebrifer (n=300) hatched larvae were randomly divided into six groups with three replicates. The degaradation efficacy tests were then performed for 30 days in a laboratory. The test groups were as follows: T1, 110 g broiler manure + T. molitor larvae (n=50); T2, 110 g duck manure + T. molitor larvae (n=50); T3, 125 g broiler manure + P. brevitarsis seulensis larvae (n=10); T4, 125 g duck manure + P. brevitarsis seulensis larvae (n=10); T5, 105 g broiler manure + P. tenebrifer larvae (n=50); and T6, 105 g duck manure + P. tenebrifer larvae (n=50). The groups showed significant efficacy in degrading broiler and duck manure (p<0.05). The highest survival rates were recorded for T. molitor larvae in both manure types [T1 (92.67%) and T2 (50%)], followed by P. brevitarsis seulensis larvae (T4, 40%) and P. tenebrifer larvae (T6, 14.67%) in duck manure. Next, the survival rates of P. brevitarsis seulensis (T3) and Ptecticus tenebrifer larvae (T5) in broiler manure were 0%. In conclusion, these results point to the feasibility of using insect larvae to degrade broiler and duck manure.

• 생명과학회지
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• 제28권7호
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• pp.827-834
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• 2018

본 연구는 유용 미생물을 이용하여 흰점박이 꽃무지 유충을 발효시킨 분말을 수용성, 에탄올, 메탄올 용매별 추출하여 항산화 등의 실험에 임하였다. 발효 균주로는 3종의 유산균 Lactobacillus plantarum JBMI F3, Lactobacillus plantarum JBMI F5, Lactobacullus gasseri Ba9, 1종의 곰팡이 Aspergillus kawachii KCCM 32819, 1종의 효모 Saccharomyces cerevisiae KACC 93023, 1 종의 바실러스 Bacillus subtilis KACC 91157 총 6종의 유용 미생물 균주를 사용하였다. 발효 굼벵이 분말 추출물의 유효성분(총 페놀 함량 및 플라보노이드 함량)과 생리 활성 효능(DPPH 자유 라디칼 소거 활성, 환원력 실험, 혈전용해능, ${\alpha}-amylase$ 활성)을 비교 검토하였다. 총 페놀 함량 및 플라보노이드 함량은 바실러스 균주 발효 굼벵이 분말의 수용성 추출물에서 높은 수치를 나타내었다. 항산화 활성을 확인하기 위해 DPPH 자유 라디칼 소거능 및 환원력 실험을 수행한 결과, 비발효군 보다 발효군에서 우수한 항산화능을 보였고, 그 중 바실러스 균주를 이용한 발효 추출물이 긍정적인 효과를 나타내었다. 혈전 용해능에서 또한, 바실러스 균주 발효 굼벵이 추출물에서 우수한 혈전 용해능을 확인였고, 효소-기질 분해능 실험의 ${\alpha}-amylase$ 활성은 비발효군보다 발효군에서 높은 효과를 확인하였으나 발효균주간 유의적인 차이는 없었다. 따라서, 유용 미생물을 이용한 발효 굼벵이 추출물의 유용성분 및 생리활성이 강화됨을 확인하였고, 이는 건강 식품 소재로의 이용 가능성이 높다고 판단된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제33권2호
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• pp.85-91
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• 2016

Exosomes are homogenous vesicles of 40-100 nm diameter produced endogenously. Exosomes are generated by inward budding into multi-vesicular bodies (MVB) and then released to extracellular space. Exosomes contain various nucleic acid and protein cargoes from their cells of origin and this endosomal cellular molecules are used for intracellular communication and for both promotion and suppression of immune responses. Recently, they are also considered as delivery vehicle for therapeutic proteins due to their characteristics of stability in body fluids and ability for target uptake. Also, they show less immune reactivity because the isolated exosome harboring therapeutic proteins can be from the same host. White-spotted flower chafer, Protaetia brevitarsis is one of the major insect commercially reared in Korea. There are bacterial and fungal pathogens causing diseases in the beetle, and these diseases incur economic loss to the larva-rearing farms. Due to their endosomal cargoes, exosomes are good candidates in use of disease diagnosis. In this study, we isolated insect exosome from the hemolymph of P. brevitarsis, and verified it by analysis of the exosome-specific surface proteins and RNA.

• 한국축산식품학회지
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• 제40권3호
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• pp.461-473
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• 2020

The goal of this study was to evaluate the influence of various supplementary feeds on the chemical composition and production of bioactive substances in Protaetia brevitarsis larvae. The primary feed-oak-fermented sawdust-was supplemented with a variety of substances, including aloe, apple, banana, sweet persimmon (S. persimmon) and sweet pumpkin (S. pumpkin). Crude protein and fat content were the highest in the control and S. pumpkin group, respectively. Supplementary feeds increased the content of unsaturated fatty acids, except in the group receiving S. pumpkin, in which oleic acid was the most abundant (58.2%-64.5%). Free essential amino acids in larvae receiving supplementary aloe were higher compared with the control group except for Lys and His. Polyphenol and flavonoid contents and the antioxidant activities of ABTS and DPPH were higher in all treated groups compared with the control group. Although supplementary feeds led to a decreased crude protein content in the treated larvae when compared with the control group, these treatments generally improved the levels of unsaturated fatty acids and antioxidative activity. Therefore, we suggest that among the supplementary foods tested, aloe is a better resource for P. brevitarsis based on crude protein content, free amino acids and other bioactive compounds such as unsaturated fatty acids and antioxidants.

• 생태와환경
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• 제53권1호
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• pp.73-79
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• 2020

Metarhizium anisopliae (녹강균)는 흰점박이꽃무지에게 질병을 일으키는 곤충 병원성 곰팡이 중 하나이다. 이러한 곰팡이의 병원성은 환경 조건에 의해 영향을 받는다. 녹강균에 대한 흰점박이꽃무지 유충의 민감성을 알기 위해 온도(20℃, 25℃, 30℃), 상대습도(RH 40%, 50%, 60%, 70%), 톱밥 수분함량(40%, 50%, 60%)이 다른 사육 조건에서 유충의 치사율을 확인하였다. 녹강균을 처리한 흰점박이꽃무지 유충의 치사율은 온도에 따라 차이가 나타나지 않았으나, 낮은 상대습도(RH 40%)와 톱밥 수분함량(40%)에서 유충의 치사율이 높았다. 녹강균에 대한 1령, 2령, 3령 유충의 치사율은 1령에서 가장 높게 나타났다.

• 한국동물학회:뉴스레터
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• 제12권2호
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• pp.107.1-107
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• 1995

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 1995년도 한국생물과학협회 학술발표대회
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• pp.243.1-243
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• 1995

No Abstract, See Full Text

• Preventive Nutrition and Food Science
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• 제18권2호
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• pp.150-156
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• 2013

A total of 48 different volatile oils were identified form P. brevitarsis larvae by gas chromatography/mass spectrometry (GC/MS). Acids (48.67%) were detected as the major group in P. brevitarsis larvae comprising the largest proportion of the volatile compounds, followed by esters (19.84%), hydrocarbons (18.90%), alcohols (8.37%), miscellaneous (1.71%), aldehydes (1.35%) and terpenes (1.16%). The major volatile constituents were 9-hexadecenoic acid (16.75%), 6-octadecenoic acid (14.88%) and n-hexadecanoic acid (11.06%). The composition of fatty acid was also determined by GC analysis and 16 fatty acids were identified. The predominant fatty acids were oleic acid ($C_{18:1}$, 64.24%) followed by palmitic acid ($C_{16:0}$, 15.89%), palmitoleic acid ($C_{16:1}$, 10.43%) and linoleic acid ($C_{18:2}$, 4.69%) constituting more than 95% of total fatty acids. The distinguished characteristic of the fatty acid profile of P. brevitarsis larvae was the high proportion of unsaturated fatty acid (80.54% of total fatty acids) versus saturated fatty acids (19.46% of total fatty acids). Furthermore, small but significant amounts of linoleic, linolenic and ${\gamma}$-linolenic acids bestow P. brevitarsis larvae with considerable nutritional value. The novel findings of the present study provide a scientific basis for the comprehensive utilization of the insect as a nutritionally promising food source and a possibility for more effective utilization.