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http://dx.doi.org/10.11614/KSL.2020.53.1.073

Pathogenicity of Entomopathogenic Fungus, Metarhizium anisopliae in Protaetia brevitarsis seulensis under Different Rearing Conditions  

Kim, Nang-Hee (Industrial Insect Division, National Institute of Agricultural Science, Rural Development Admistration)
Kim, Eunsun (Industrial Insect Division, National Institute of Agricultural Science, Rural Development Admistration)
Song, Myung-Ha (Industrial Insect Division, National Institute of Agricultural Science, Rural Development Admistration)
Kawk, Kyu-Won (Industrial Insect Division, National Institute of Agricultural Science, Rural Development Admistration)
Park, Kwan-Ho (Industrial Insect Division, National Institute of Agricultural Science, Rural Development Admistration)
Kim, Yongsoon (Industrial Insect Division, National Institute of Agricultural Science, Rural Development Admistration)
Publication Information
Korean Journal of Ecology and Environment / v.53, no.1, 2020 , pp. 73-79 More about this Journal
Abstract
Metarhizium anisopliae is one of the entomopathogenic fungi infecting Protaetia brevitarsis seulensis. Environmental conditions strongly affect the virulence of entomopathogenic fungus. To test the susceptibility of P. brevitarsis seulensis larvae to M. anisopliae, we determined level mortality rates under different rearing conditions: temperatures (20℃, 25℃, 30℃), relative humidities (RH; RH 40%, 50%, 60%, 70%), and water content in sawdust (40%, 50%, 60%). The mortality rate of larvae treated with 5×106 conidia mL-1 M. anisopliae was not significantly affected by rearing temperature, but was affected by low RH (40%) and low water content in sawdust (40%), both of which resulted in high mortality rates. Mortality rated among M. anisopliae-treated 1st, 2nd, and 3rd instar larvae were highest in 1st instar larvae.
Keywords
entomopathogenic fungi; Protaetia brevitarsis seulensis; Metarhizium anisopliae; temperature; humidity;
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1 Akbar, W., J.C. Lord, J.R. Nechols and R.W. Howard. 2004. Diatomaceous earth increases the efficacy of Beauveria bassiana against Tribolium castaneum larvae and increases conidia attachment. Journal of Economic Entomology 97:273-280.   DOI
2 Anand, R., B. Presad and B.N. Tiwary. 2009. Relative susceptibility of Spodoptera litura pupae to selected entomopathogenic fungi. Biocontrol 54: 85-92.   DOI
3 Athanassiou, C.G., N.G. Kavallieratos, C.I. Rumbos and D.C. Kontodimas. 2017. Influence of temperature and relative humidity on the insecticidal efficacy of Metarhizium anisopliae against larvae of Ephestia kuehniella (Lepidoptera:Pyralidae) on wheat. Journal of Insect Science 17: 1-7.   DOI
4 Douro-Kpindou, O.K., D.A. Djegui, I.A. Glitho and M. TamO. 2012. Sensitivity of Helicoverpa armigera (Hubner) (Lepidoptera:Noctuidae) to the entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana in laboratory. Journal of Agriculture and Biology Science 7: 1007-1015.
5 Erler, F. and A.O. Ates. 2015. Potential of two entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Coleoptera: Scarabaeidae), as biological control agents against the June beetle. Journal of Insect Science 15: 44.   DOI
6 Gillespie, A.T. and E. Crawford. 1986. Effect of water activity on conidial germination and mycelial growth of Beauveria bassiana, Metarhizium anisopliae, Paecilomyces spp. and Verticillium lecanii, p. 254. In fundamental and applies aspects of invertebrate pathology (Proceedings of the fourth international colloquium of invertebrate pathology) (Samson, R.A., J.M. Vlak and D. Peters, eds.).
7 Goettel, M.S., J. Eilenberg and T. Glare. 2005. Entomopathogenic fungi and their role in regulation of insect populations, p. 361-405. In: Molecular insect science (Gilbert, L.K., K. Iatrou, S.S. Gill, eds.). Elservier, New York, USA.
8 Hafez, M., F.N. Zaki, A. Moursy and M. Sabbour. 1997. Biological effects of the entomopathogenic fungus, Beauveria bassiana on the potato tuber moth Phthorimaea operculella (Seller). Journal of Pest Science 70: 158-159.
9 Hammer, O., D.A.T. Harper and P.D. Ryan. 2001. PAST: Paleontological statistics software package for education and data analysis. Paleaeontologica Electronica 4: 1-9.
10 Hall, R.A. 1981. The fungus Verticillium lecanii as a microbial insecticide against aphids and scales, p. 483-498. In: Microbial control of pests and plant diseases 1970-1980 (Burges H.D. ed.). Academic press, London.
11 Han, J.H., J.J. Kim and S.Y. Lee. 2014. Insecticidal activity of Metarhizium anisopliae FT83 against the different stages of beet armyworm, Spodoptera exigua. Korean Journal of Pesticide Science 18: 417-421.   DOI
12 Hussain, A., M.Y. Tian, Y.R. Ho and S. Ahmed. 2009. Entomopathogenic fungi disturbed the larval growth and feeding performance of Ocinara varians (Lepidoptera: Bombycidae) larvae. Insect Science 16: 511-517.   DOI
13 Hywel-Jones, N.L. and A.T. Gillespie. 1990. Effect of temperature on spore germination in Metarhizium anisopliae and Beauveria bassiana. Mycological Research 94: 389-392.   DOI
14 Indriyanti, D.R., P. Widiyaningrum, Haryuni, M. Slamet and Y.A. Maretta. 2017. Effectiveness of Metarhizium anisopliae and entomopathogenic nematodes to control Oryctes rhinoceros larvae in the rainy season. Parkistan Journal of Biological Sciences 20: 320-327.   DOI
15 Lee, M.R., J.C. Kim, S.J. Lee, S. Kim, S.J. Lee, S.E. Park, W.H. Lee and J.S. Kim. 2017. Assessment of physiological activity of entomopathogenic fungi with insecticidal activity against locusts. Korean Journal of Applied Entomology 56:301-308.   DOI
16 Kim, H.G. and K.H. Kang. 2006. Imago's flight and larval activites of Protaetia brevitarsis (Coleoptera: Scarabaeidae) and Allomyrian dichotoma (Coleoptera: Dynastinae). Korean Journal of Applied Entomology 45: 139-143.
17 Kim, H.G., K.H. Kang and C.Y. Hwang. 2005. Effect of some environmental factors on oviposition and developmental characteristic of Protaetia brevitarsis and Allomyrina dichotoma. Korean Journal of Applied Entomology 44: 283-286.
18 Lee, J.B. and Y.J. Park. 2015. Insecticidal effect of entomopathogenic fungus, Beauveria bassiana ANU1 to Spodoptera exigua and Plutella xylostella by different temperature and humidity conditions. The Korean Society of Pesticide Science 19: 125-133.   DOI
19 Lord, J.C. 2005. Low humidity, moderate temperature, and desiccant dust favor efficacy of Beauveria bassiana (Hyphomycetes:Moniliales) for the lesser grain borer, Rhyzopertha dominica (Coleoptera: Bruchidae). Biological Control 34:180-186.   DOI
20 Luz, C. and J. Fargues. 1997. Temperature and moisture requirement for conidial germination of an isolate of Beauveria bassiana, pathogenic to Rhodnius prolixus. Mycopathologia 138: 117-125.   DOI
21 Mitchell, M.J. and A. Cali. 1994. Vairimorpha necatrix (Microsporidia:Burenellidae) affects growth and development of Heliothis zea (Lepidoptera: Noctuidae) raised at various temperatures. Journal of Economic Entomology 87: 933-940.   DOI
22 Mittal, I.C. 2000. Survey of Scarabaeid (Coleoptera) fauna of Himanchal Pradesh (India). Journal of Entomological Research 24: 133-141.
23 Saleem, A.R. and R.A. Ibrahim. 2019. Assessment of the virulence and proteolytic activity of three native entomopathogenic fungi against the larvae of Oryctes Agamemnon (Burmeister) (Coleoptera: Scarabaeidae). Egytpian Journal of Biological Pest Control 29: 21.   DOI
24 Park, H.Y., S.S. Park, H.W. Oh and J.I. Kim. 1994. General characteristics of the white-spotted flower chafer, Protaetia brevitarsis reared in the laboratory. Korean Journal of Entomology 24: 1-5.
25 Riddick, E.W. and Z. Wu. 2015. Effects of rearing density on survival, growth, and development of the ladybird Coleomegilla maculata in culture. Insects 6: 858-868.   DOI
26 Roberts, D.W. and R.J. St. Leger. 2004. Metarhizium spp., Cosmopolitan Insect-pathogenic Fungi: Mycological Aspects. Advances in Applied Microbiology 54: 1-70.   DOI
27 Shah, P.A. and J.K. Pell. 2003. Entomopathogenic fungi as biological control agents. Applied Microbiology and Biotechnology 61: 413-423.   DOI
28 Sharififard, M., M.S. Mossadegh and B. Vazirianzadeh. 2012. Effects of temperature and humidity on the pathogenicity of the entomopathogenic fungi in control of the house fly, Musca domestica L. (Diptera: Muscidae) under laboratory conditions. Journal of Entomology 9: 282-288.   DOI
29 Shin, M.K. 1986. Clinical traditional herbalogy. Young Lim Press Co. Seoul (Korea). p. 482.
30 Song, M.H., M.H. Han, S. Lee, E.S. Kim, K.H. Park, W.T. Kim and J.Y. Choi. 2017. A field survey on edible insect farms in Korea. Journal of Life Science 27: 702-707.   DOI