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http://dx.doi.org/10.7852/ijie.2016.33.2.85

Exosome isolation from hemolymph of white-spotted flower chafer, Protaetia brevitarsis (Kolbe) (Coleoptera: Scarabaeidae).  

Lee, Seokhyun (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Kwon, Kisang (Department of Biomedical Laboratory Science, College of Health and Welfare, Kyungwoon University)
Song, Myung-Ha (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Park, Kwan-ho (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Kwon, O-Yu (Department of Anatomy, College of Medicine, Chungnam National University)
Choi, Ji-young (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.33, no.2, 2016 , pp. 85-91 More about this Journal
Abstract
Exosomes are homogenous vesicles of 40-100 nm diameter produced endogenously. Exosomes are generated by inward budding into multi-vesicular bodies (MVB) and then released to extracellular space. Exosomes contain various nucleic acid and protein cargoes from their cells of origin and this endosomal cellular molecules are used for intracellular communication and for both promotion and suppression of immune responses. Recently, they are also considered as delivery vehicle for therapeutic proteins due to their characteristics of stability in body fluids and ability for target uptake. Also, they show less immune reactivity because the isolated exosome harboring therapeutic proteins can be from the same host. White-spotted flower chafer, Protaetia brevitarsis is one of the major insect commercially reared in Korea. There are bacterial and fungal pathogens causing diseases in the beetle, and these diseases incur economic loss to the larva-rearing farms. Due to their endosomal cargoes, exosomes are good candidates in use of disease diagnosis. In this study, we isolated insect exosome from the hemolymph of P. brevitarsis, and verified it by analysis of the exosome-specific surface proteins and RNA.
Keywords
Protaetia brevitarsis; hemolymph; exosome; exosome protein; exosome RNA;
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