• Microbiology and Biotechnology Letters
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• v.17 no.1
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• pp.19-23
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• 1989

A sequential system composed of hydroxylapatite chromatography and gel permeation chromatography was developed to purify the IgM type monoclonal antibody against the colon cancer cell SC-1 from the ascitic fluid of mice injected with the murine hybridoma CH07E02. In the hydroxylapatite chromatographic step the band dilution could be reduced by controlling the gradient and flow rate of the eluent, the sodium phospate buffer, the optimum values for these variables being 5.82$\times$10$^{-3}$M/cm and 0.2$m\ell/\textrm{cm}^2$/min, respectively. A degree of purity better than 99.99% as judged from silverstaining of the SDS-PAGE bands, was obtained by adding the gel permeation chromatographic step in tandem.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.4
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• pp.523-533
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• 2019

The convergence of engineering and IT technology has brought many changes to the industry as well as academic research. In particular, computer simulation technology has evolved to a level that can accurately simulate actual physical phenomena and analyze them in real time. In this paper, we describe the CFD technology, which is mainly used in industry, and the post processor that uses the augmented reality which is emerging as the post-processing. Research on the visualization of fluid simulation results using AR technology is actively being carried out. However, due to the large size of the result data, it is limited to researches that are published in a desktop environment. Therefore, it is limitation that needs to be reviewed in actual space. In this paper, we discuss how to solve these problems. We analyze the fluid analysis results in the post-processing, and then perform optimizing data (more than 70%)to support operation in the mobile environment. In the visualization, lightweight data is used to perform real-time tracking using cloud computing, The analysis result is matched to the screen and visualized. This allows the user to review and analyze the fluid analysis results in an efficient and immersive manner in the various spaces where the simulation is performed.

• Journal of the Korean Applied Science and Technology
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• v.31 no.1
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• pp.143-150
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• 2014

Recently, supercritical fluid process has been widely used in material synthesis and processing due to their remarkable properties such as high diffusivity, low viscosity, and low surface tension. Supercritical carbon dioxide is the most attractive solvent owing to their characteristics including non-toxic, non-flammable, chemically inert, and also it has moderate critical temperature and critical pressure. In addition, supercritical carbon dioxide would dissolve many small organic molecules and most polymers. In this study, we have prepared the poly (ethylene oxide)/clay nanocomposites using supercritical fluid as a carbon dioxide. Commercialized Cloisites-15A and Cloisites-30B used in this study, which are modified with quaternary ammonium salts. The nanocomposites of polymer/clay were characterized by XRD, TGA and DSC. Poly (ethylene oxide)/clay nanocomposites by supercritical fluid show higher thermal stability than nanocomposites prepared by melt process. In addition, supercritical fluid process would be increased dispersibility of the nanoclay in the matrix.

• Journal of The Korean Society of Agricultural Engineers
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• v.47 no.5
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• pp.27-39
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• 2005

In the recent design of high ductile fiber-reinforced ECC (engineered cementitious composite), optimizing both processing and mechanical properties for specific applications is critical. This study presents an innovative method to develop new class ECCs, which possess the different fluid properties to facilitate diverse types of processing (i.e., self-consolidating or shotcrete processing) while maintaining ductile hardened properties. In the material design concept, we employ a parallel control of fresh and hardened properties by using micromechanics and cement rheology. Control of colloidal interaction between the particles is regarded as a key factor to allow the performance of the specific processing. To determine how to control the particle interactions and the viscosity of cement suspension, we first introduce two chemical admixtures including a highly charged polyelectrolyte and a non-ionic polymer. Optimized mixing steps and dosages we, then, obtained within the solid concentration predetermined based on micromechanical principle. Test results indicate that the rheological properties altered by this approach were revealed to be highly effective in obtaining the desired function of the fresh ECC, allowing us to readily achieve hardened properties, represented by pseudo strain-hardening behavior in uniaxial tension.

• Journal of Powder Materials
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• v.15 no.1
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• pp.46-52
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• 2008

The restrictor, which is a fluid channel from a reservoir to a chamber inside a thermal micro actuator, has been fabricated using ArF and KrF excimer lasers, Diode-Pumped Solid State Lasers (DPSSL) and femtosecond lasers for a feasibility study. A numerical model of fluid dynamics for the actuator chamber and restrictor is presented. The model includes bubble formation and growth, droplet ejection through nozzle, and dynamics of fluid refill through the restrictor from a reservoir. Since an optimized and well-fabricated restrictor is important for a high frequency actuator, some special beam delivery setups and post processing techniques have been researched and developed. The effects of variations of the restrictor length, diameter, and tapered shapes are simulated and the results are analyzed to determine the optimal design. The numerical results of droplet velocity and volume are compared with the experimental results of a cylindrical-shaped actuator. It is found that the micro actuators having tapered restrictors show better high frequency characteristics than those having a cylindrical shape without any notable decrease of droplet volume. The laser-fabricated restrictors demonstrate initial feasibility for the laser direct ablation technique although more development is required.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.24 no.4
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• pp.504-511
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• 2000

PIV(Particle Image Velocimetry) is a recently developed technique for visualizing the fluid velocity fields. Because it has several advantages over the LDV(Laser Doppler Velocimetry), it became one of the most popular diagnostic tools in spite of its short history. However, its application to combustion is restricted by some problems such as flame illumination, scattered light refraction, particle density variation due to heat release, the combined effect of abrupt change in particle density and fluid velocity on flame contour, and thermophoresis which is particle lagging due to temperature gradient. These problems are expected to be originated from the non-continuous characteristics of flames and the limitations of particle dynamics. In the present study, these problems were considered for the visualization of the instantaneous coaxial hydrogen diffusion flame. And the instantaneous flame contour was detected using particle density difference. The visualized diffusion flame velocity field shows its turbulent and meandering nature. It was also observed that the flame is located inside the outer shear layer and flame geometry is largely influenced by the vorticity.

• The KSFM Journal of Fluid Machinery
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• v.19 no.4
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• pp.39-42
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• 2016

A PFA lined ball valve, which is machined with fluorinated resin PFA to its inner part for improving corrosion resistance, non-stickness, heat-resistance, has been widely used to the chemical/pharmaceutical industries, the semiconductor/LCD manufacturing processes, etc. with the high purity chemicals as working fluid. EPA stated that 60% of all fugitive emissions come from the valve stem packing in a typical petroleum or chemical processing plant. They monitor regulated components for leaks and maintain seal performance at acceptable levels. Korean industrial standards only deals with the bubble test for in-line leakage of valves, which has the detectable leak rate of $10^{-4}$ [$mbar{\cdot}L{\cdot}s^{-1}$], therefore, it is not sufficient to check fugitive emissions. In this study, we conducted Helium leak detection from a PFA lined ball valve and evaluated fugitive emissions according to ISO 15848-1, which has the detectable leak rate of $10^{-9}$ [$mbar{\cdot}L{\cdot}s^{-1}$], for manufacturing the high-reliable PFA lined ball valves against fugitive emissions.

• Journal of Ship and Ocean Technology
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• v.4 no.3
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• pp.1-12
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• 2000

A number of numerical methods like Computational Fluid Dynamics(CFD) have been developed to predict the flow fields of a vessel but the present study is developed to infer the wake fields on propeller plane by Statistical Fluid Dynamics(SFD) approach which is emerging as a new technique over a wide range of industrial fields nowadays. Neural network is well known as one prospective representative of the SFD tool and is widely applied even in the engineering fields. Further to its stable and effective system structure, generalization of input training patterns into different classification or categorization in training can offer more systematic treatments of input part and more reliable result. Because neural network has an ability to learn the knowledge through the external information, it is not necessary to use logical programming and it can flexibly handle the incomplete information which is not easy to make a definition clear. Three dimensional stern hull forms and nominal wake values from a model test are structured as processing elements of input and output layer respectively and a neural network is trained by the back-propagation method. The inferred results show similar figures to the experimental wake distribution.

• Food Science and Biotechnology
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• v.27 no.6
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• pp.1857-1864
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• 2018

Phthalate plasticizers residue in food is a serious threat to public health. Spores of Ganoderma lucidum are easy to be contaminated with phthalates during collection and processing. In this study, supercritical fluid extraction (SFE) was performed to remove phthalates in spores of G. lucidum, and the effects on acid and peroxide values of spores' oil were also evaluated. The results showed SFE removed 100% of the residual di-iso-butyl phthalate, di-n-butyl phthalate and di-2-ethylhexyl phthalate in the spores of G. lucidum. No significant differences in polysaccharides content and fatty acid composition were observed between SFE and control spores. However, the triterpenoid extracts of SFE spores had a 7.45% increase, significantly higher than that in control spores. Accelerated oxidation tests further implied that SFE could improve the stability of spores' oil. Our results suggested SFE is a potential approach to remove phthalate from food related products.

• Proceedings of the KSME Conference
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• 2001.06d
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• pp.509-515
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• 2001

In a square cavity, the flow phenomena in the surrounding of the bubble attached at the upper cooled solid wall were studied by using a thermo-sensitive liquid-crystal tracer and image processing techniques. This method offers the advantage of measuring the entire flow field in a selected plane within the fluid at a given instant of time in contrast to point by point method like T/C. Quantitative data of the temperature were obtained by applying a colour-image-processing to the. visualized image. As the growing of a bubble, In a bubble size appears the flow phenomena which the direction of flow is reversed in the entire temperature and flow field. The observed phenomena are described with regard to thermocapillary convection.