• Journal of Microbiology and Biotechnology
• /
• 제32권2호
• /
• pp.238-247
• /
• 2022

Since the skin covers most surfaces of the body, it is susceptible to damage, which can be fatal depending on the degree of injury to the skin because it defends against external attack and protects internal structures. Various types of artificial skin are being studied for transplantation to repair damaged skin, and recently, the production of replaceable skin using three-dimensional (3D) bioprinting technology has also been investigated. In this study, skin tissue was produced using a 3D bioprinter with human skin cell lines and cells extracted from mouse skin, and the printing conditions were optimized. Gelatin was used as a bioink, and fibrinogen and alginate were used for tissue hardening after printing. Printed skin tissue maintained a survival rate of 90% or more when cultured for 14 days. Culture conditions were established using 8 mM calcium chloride treatment and the skin tissue was exposed to air to optimize epidermal cell differentiation. The skin tissue was cultured for 14 days after differentiation induction by this optimized culture method, and immunofluorescent staining was performed using epidermal cell differentiation markers to investigate whether the epidermal cells had differentiated. After differentiation, loricrin, which is normally found in terminally differentiated epidermal cells, was observed in the cells at the tip of the epidermal layer, and cytokeratin 14 was expressed in the lower cells of the epidermis layer. Collectively, this study may provide optimized conditions for bioprinting and keratinization for three-dimensional skin production.

• 한국환경과학회지
• /
• 제29권5호
• /
• pp.479-494
• /
• 2020

This study analyses the characteristics of volatile organic compounds (VOCs) emissions from the painting and printing facilities, as well as ambient VOCs at industrial complexes in Gwangju. The major components of VOCs emissions from painting facilities were toluene, acetone, 2-butanone, ethyl acetate, ethyl benzene, o-xylene and m,p-xylene. The printing facilities mostly emitted ethyl acetate, 2-butanone, acetone and toluene. Aromatics (49.9%) and oxygenated VOCs (43.6%) were dominant in painting facilities, while oxygenated VOCs (92.7%) were the largest group in printing facilities. The total hydrocarbon concentration (THC) in printing facilities was approximately six times higher than in the painting facilities. The painting and printing facilities use many solvents. Their THC concentrations differed considerably depending on the type of prevention facilities. To reduce THC, it is necessary to improve the prevention facilities and operating conditions. The dominant species of ambient VOCs in industrial complexes were investigated with toluene, ethyl acetate, 2-butanone, ethyl benzene, m,p-xylene, butyl acetate, o-xylene, hexane and acetone. Factor analysis of ambient VOCs showed that the main sources of the VOCs were organic solvents used in painting, coating, and printing, as well as automobile emissions.

• 한국정보디스플레이학회:학술대회논문집
• /
• 한국정보디스플레이학회 2007년도 7th International Meeting on Information Display 제7권1호
• /
• pp.380-383
• /
• 2007

In this research, we present color filter which is patterned by screen printing method. Analysis of screen printing process, screen printing system, experiment for uniform printing in large area, characteristics of screen printed CF are investigated .In spite of limitation of precision, screen printed color filter is very cost- effective in respect of manufacturing facility and ink usage.

• 한국재료학회지
• /
• 제24권2호
• /
• pp.73-80
• /
• 2014

Ink-jet printing technology has been widely attractive due to its facility for direct and fine printing on various substrates. Recent studies have focused on expanding the application of ink-jet printing technology from general consumer use and design companies to the prototype production of precision parts and parts manufacturing. The use of ink-jet printing technology in decorated tableware, tiles, and other ceramic products also has many advantages. The printing process is fast and can be adaptable to various kinds of objects because there is no direct contact point between the printer and the substrates to be printed. For application to ceramic product decoration, inks containing highly dispersed inorganic nano-pigments are required. Here we report the synthesis and characterization of blue $CoAl_2O_4$ nanopigment for ink-jet printing. Blue ceramic ink based on the obtained $CoAl_2O_4$ pigment was prepared by dissolving $CoAl_2O_4$ pigment in a mixed solution of ethylene glycol and ethanol with volume ratios of 7:3 and 8:2, respectively, to obtain the appropriate viscosity for ink-jet printing. The ink solution contained 15 wt% of $CoAl_2O_4$ pigment and Cetyltrimethyl ammonium bromide(CTAB) and Sodium dodecyl sulfate(SDS) as dispersive agents. The prepared blue ceramic ink was stably jetted and formed a sphere-shaped droplet from an ink-jet printer.

• Nuclear Engineering and Technology
• /
• 제53권3호
• /
• pp.776-792
• /
• 2021

Conventional integral effect test facilities were constructed to enable the precise observation of thermal-hydraulic phenomena and reactor behaviors under postulated accident conditions to prove reactor safety. Although these facilities improved the understanding of thermal-hydraulic phenomena and reactor safety, applications of new technologies and their performance tests have been limited owing to the cost and large scale of the facilities. Various nuclear technologies converging 4th industrial revolution technologies such as artificial intelligence, drone, and 3D printing, are being developed to improve plant management strategies. Additionally, new conceptual passive safety systems are being developed to enhance reactor safety. A new integral effect test facility having a noticeable scaling ratio, i.e., the (UNIST reactor innovation loop (URI-LO), is designed and constructed to improve the technical quality of these technologies by performance and feasibility tests. In particular, the URI-LO, which is constructed using a transparent material, enables better visualization and provides physical insights on multidimensional phenomena inside the reactor system. The facility design based on three-level approach is qualitatively validated with preliminary analyses, and its functionality as a test facility is confirmed through a series of experiments. The design feature, design validation, functionality test, and future utilization of the URI-LO are introduced.

• Safety and Health at Work
• /
• 제10권4호
• /
• pp.518-526
• /
• 2019

Background: Additive manufacturing (AM) is a rapidly expanding new technology involving challenges to occupational health. Here, metal exposure in an AM facility with large-scale metallic component production was investigated during two consecutive years with preventive actions in between. Methods: Gravimetric analyzes measured airborne particle concentrations, and filters were analyzed for metal content. In addition, concentrations of airborne particles <300 nm were investigated. Particles from recycled powder were characterized. Biomonitoring of urine and dermal contamination among AM operators, office personnel, and welders was performed. Results: Total and inhalable dust levels were almost all below occupational exposure limits, but inductively coupled plasma mass spectrometry showed that AM operators had a significant increase in cobalt exposure compared with welders. Airborne particle concentrations (<300 nm) showed transient peaks in the AM facility but were lower than those of the welding facility. Particle characterization of recycled powder showed fragmentation and condensates enriched in volatile metals. Biomonitoring showed a nonsignificant increase in the level of metals in urine in AM operators. Dermal cobalt and a trend for increasing urine metals during Workweek Year 1, but not in Year 2, indicated reduced exposure after preventive actions. Conclusion: Gravimetric analyses showed low total and inhalable dust exposure in AM operators. However, transient emission of smaller particles constitutes exposure risks. Preventive actions implemented by the company reduced the workers' metal exposure despite unchanged emissions of particles, indicating a need for careful design and regulation of the AM environments. It also emphasizes the need for relevant exposure markers and biomonitoring of health risks.

• 산업경영시스템학회지
• /
• 제44권4호
• /
• pp.32-42
• /
• 2021

The printing process can have to print various colors with a limited capacity of printing facility such as ink containers that are needed cleaning to change color. In each container, cleaning time exists to assign corresponding inks, and it is considered as the setup cost required to reduce the increasing productivity. The existing manual method, which is based on the worker's experience or intuition, is difficult to respond to the diversification of color requirements, mathematical modeling and algorithms are suggested for efficient scheduling. In this study, we propose a new type of scheduling problem for the printing process. First, we suggest a mathematical model that optimizes the color assignment and scheduling. Although the suggested model guarantees global optimality, it needs a lot of computational time to solve. Thus, we decompose the original problem into sequencing orders and allocating ink problems. An approximate function is used to compute the job scheduling, and local search heuristic based on 2-opt algorithm is suggested for reducing computational time. In order to verify the effectiveness of our method, we compared the algorithms' performance. The results show that the suggested decomposition structure can find acceptable solutions within a reasonable time. Also, we present schematized results for field application.

• 한국환경과학회지
• /
• 제32권6호
• /
• pp.403-417
• /
• 2023

In this study, we investigated the characteristics of Volatile Organic Compounds(VOCs) emission from painting and printing facilities in the Pyeongdong industrial complex in Gwangju. In addition, the objective was to understand the distribution characteristics of VOCs in the ambient air in industrial complexes affected by painting and printing facilities. The painting facility mainly emitted toluene, acetone, butyl acetate, 4-methyl-2-pentanone, ethyl acetate, 1-butanol, methyl ethyl ketone, m,p-xylene, o-xylene, 4-ethyltoluene, ethylbenzene, 3-ethyltoluene, and 1,2,4-trimethylbenzene. The main emission components in printing facilities were methyl ketone, ethyl acetate, acetone, 2-propanol, toluene, heptane, and butyl acetate. Ethyl acetate, toluene, 2-butanone, acetone, butyl acetate, 2-propanol, xylenes, and 4-methyl-2-pentanone were detected in the ambient air of the Pyeongdong industrial complex, consistent with the VOCs emitted by painting and printing facilities. The average concentration of seasonal TVOCs followed an order of winter > fall > spring > summer, whereas the concentrations of daytime and nighttime TVOCs were generally higher at night than those during the day, and the wind speed was greater during the day than it was at night. Based on a factor analysis of VOCs in the ambient air of Pyeongdong industrial complex, it is considered that organic solvents used in coating, printing, and electronics manufacturing facilities, as well as diesel vehicle emissions played a major role.

• Applied Microscopy
• /
• 제35권1호
• /
• pp.1-13
• /
• 2005

전자현미경 관찰에 있어서 최종적인 목표는 정확한 정보를 포함하는 좋은 사진을 얻는 것이라 할 수 있다. 이를 위하여는 사진에 인공물이 포함되지 않도록 표본의 채취, 처리, 관찰 및 사진 작업 등 모든 단계에서 주의를 기울여야 한다. 본 고에서는 통상적인 전자현미경 관찰을 위한 시료의 채취로부터 사진의 현상, 인화에 이르는 여러 단계에서 발생될 수 있는 인공물들과 그 원인을 살펴 보고 해결책을 제시함으로써 전자현미경 관찰을 위한 표본의 올바른 제작과 미세구조의 해석에 도움을 주고자 하였다.

• 한국정보통신학회논문지
• /
• 제22권7호
• /
• pp.1001-1008
• /
• 2018

본 논문은 인명구조, 사고방지, 시설관리 등 수상환경에서 위치정보가 필요한 상황에서 무선 신호를 활용한 효율적인 위치 추적 기술을 제안한다. 제안하는 기법은 유저의 배터리 파워 소모량을 고려하여 제한된 전력 내에서 위험도를 최소화한다. 수상의 일정 반경 내에서 유저의 송수신 신호 세기를 이용하여 유저의 위치를 알 수 있는데, 이 때 유저가 일정 반경을 벗어나는 것을 사전에 방지할 필요가 있다. 이 논문에서는 먼저 유저가 일정 반경을 벗어나는 것을 방지하기 위해 위험도 함수를 정의한다. 위험도 함수는 위치정확도, 수심, 유속 등 수상 환경 위험에 관련된 다양한 요인들을 고려한다. 본 논문은 finger printing 기법을 이용한 효율적인 무선 측위 기술을 활용하여 일정 지역 내에 위치한 유저의 평균 위험도를 최소화하는 것을 목표로 하고 있다.