• Preventive Nutrition and Food Science
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• 제4권2호
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• pp.122-124
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• 1999

Induction of NAD(P)H : (quinone-acceptor) oxidoreductase (QR) which obligatory two electron reduction of quinones and prevents their participation in oxidative cycling and thereby the depletion of intracellular glutathione, has been used as a marker for chemopreventive agents. We postulated that vitamin E, an antioxidant, which induces QR as the gene of QR was reported to contain antioxidant reponsive element in the 5'-flanking region. Vitamin E resulted in significant induction of QR in both hepalclc7 cells and mouse tissues. QR induction was observed; to be maximal at 25uM vitamin E for hepalclc7 cells while it was maximal in the level of 2.5∼5 μmoles vitamin E/㎏ BW for mouse tissues. Thus the cancer-preventive effect of vitamin E may be exerted by it induction of intracellular detoxifying enzymes.

• 농촌의학ㆍ지역보건
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• 제44권1호
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• pp.39-42
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• 2019

Zoonoses are the diseases that are transmitted to human being from vertebrate animals either from livestock animals or from wildlife. Recently, zoonoses are increasingly common as a result of incremental human-animal contact. Propagative infections in wild animals and livestock are transmitted to human beings who are encountered with them. In general, wild animals can transmit infectious agents to livestock, and then livestock further transmit them to human being is a simple model of on how zoonotic diseases get transmitted to human being. This model emphasizes the importance of early detection of zoonoses by surveillance at its incipient stage. Cooperation between the respective ministries plays an important role in the identification of zoonoses and planning for the formulation of better preventive and control policy and strategy. We will be able to predict the occurrence of zoonotic diseases in human on the basis of disease trends in wildlife and livestock once when we obtain the surveillance data and data generated by respective ministries through sound cooperation and collaboration.

• Journal of Preventive Veterinary Medicine
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• 제42권4호
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• pp.177-181
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• 2018

Disulfiram is a drug used to treat alcohol dependence. Recent studies have shown that disulfiram also has anti-cancer effects. Considering that many anti-cancer agents have side effects, including immunosuppression, it is important to check if disulfiram has some cytotoxicity to immune cells. In this study, mouse spleen cells were treated with disulfiram and the metabolic activity was measured. Disulfiram increased the cell death of spleen cells according to annexin V-FITC/PI staining analysis. In addition, disulfiram decreased the mitochondrial membrane potential of spleen cells. The toxicity of disulfiram was concentration dependent. Interestingly, disulfiram affected the population of lymphocytes and the subset of spleen cells was altered. This study provides clinicians and researchers with valuable information regarding the toxicity of disulfiram to mouse spleen cells, particularly lymphocytes.

• 동의생리병리학회지
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• 제20권3호
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• pp.557-562
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• 2006

Hypoxia induced angiogenesis and inflammation are essential processes for metastasis and progress of solid tumors. We examined the anti-angiogenic and inflammation related activity of Kochiae Fructus (KF) extract. To investigate the roles of the KF extract, we performed MTS assay, western blots using HaCaT cells and $HepG_2$ cells. The results are as follows. The protein level of $HIF-1{\alpha}$ was reduced when induced by $CoCl_2$ in $HepG_2$ cells treated with KF extract and induced by IGF-11 in HaCaT cells treated with KF extract. KF extract reduced the mRNA level of VEGF in HaCaT cells and KF extract reduced the protein level of iNOS in HaCaT cells. These results suggest that KF extract contributes to the anti-angiogenic and anti-inflammatory activities and also we could assume that KF extract act as antioxidant or anti-inflammatory agents via reduction of $HIF-1{\alpha}$.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2001년도 International Symposium on Dietary and Medicinal Antimutgens and Anticarcinogens
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• pp.35-36
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• 2001

Nutrition influences cancer incidence and offers a variety of preventive dietary factors including non-nutritive plant metabolites. To identify novel potential chemopreventive agents, we have set up cell- and enzyme-based in vitro marker systems relevant for prevention of carcinogenesis in vivo. This experimental approach led to the identification of Xanthohumol (Xn), a prenylated chalcone from hop (Humulus lupulus L.) as a most promising broad-spectrum chemopreventive agent.(omitted)

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2001년도 Korean Environmental Mutagen Society
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• pp.22-23
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• 2001

• Journal of Preventive Medicine and Public Health
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• 제29권3호
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• pp.597-607
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• 1996

Genotoxic agents can induce various DNA lesions. DNA-Protein Crosslinks(DPCs) were known as the important DNA lesions which could impair gene expression because DPCs had a high probability of resisting repair and persisting through cell cycle. This repair resistance of DPCs could have biological significance but had not been evaluated clearly yet. Most of the studies that have evaluated the repair of DPCs only compared the extent of DPCs repair with other DNA lesions. We injected $K_2CrO_4$, a genotoxic agent, into Sprague-Dawley rats intraperitoneally(5mg/kg) and isolated blood lymphocytes 12 hours later. These lymphocytes were cultured in the mitogen added growth media and mitogen free media separately. The degree of the repair of DPCs was monitored for 4 days by the K-SDS assay. 4 days later, the amount of DPCs decreased by 4.6% in the mitogen added media high increased by 10.9% in the mitogen free media. These results showed that DPCs induced by $K_2CrO_4$ were not repaired easily and the DPCs were biologically significant DNA lesions. We thought the decrease of DPCs in the mitogen added media was not due to the repair of DPCs, but from the increase of normal cell proliferation. Therefore, it is very important to consider the proliferation of normal cells when estimating the repair of DPCs.

• Journal of Preventive Medicine and Public Health
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• 제19권2호
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• pp.184-192
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• 1986

Since the wide spread application of hyperbaric oxygenation in clinical setting, the problems of oxygen toxicity have been attracting a deep interest from the researchers on hyperbaric medicine as a practical issue. Among extensive research trials, the study on the protective agents against oxygen toxicity occupied one of the most challenging field. As the mechanisms of oxygen toxicity, the role of the oxygen free radicals produced by peroxidation process are strongly accepted by the leading researchers on oxygen toxicity, the probable protective effects of antioxidant against oxygen toxicity are sustaining a sufficient rationale. In this study, the author attempted to evaluate the effect of vitamin E as a protective agent against oxygen toxicity through the observation of death rate, convulsion rate, time to convulsion, and macroscopic and microscopic pathological changes of experimental rats exposed to 100% oxygen at 5 ATA for 120 minutes. The findings observed are as follows: 1) The death rate, convulsion rate, time to convulsion, organ/body weight ratio and microscopic pathological findings were identified as reliable objective and quantitative indices for oxygen toxicity. 2) Vitamin E showed excellent protective effects against CNS and pulmonary oxygen toxicity as a strong antioxidant. The most effective dose seemed to be around 400 mg/kg 3) The results of this study are supporting the oxygen free radical hypothesis on oxygen toxicity.

• Journal of Preventive Medicine and Public Health
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• 제29권1호
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• pp.1-14
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• 1996

한 종합병원의 작업환경에 존재하는 건강저해인자를 평가함으로써 종사원들의 건강보호와 작업능률 향상에 기여하고자 조사연구를 실시하였다. 병원건물의 구조와 업무내용으로 보아 작업환경의 대표가 될 것으로 보이는 27개 부서를 선정하여 1993년 10월부터 1994년 10월까지 작업환경에 대한 종사원의 설문과 현장조사 및 측정을 실시하였다. 설문을 통하여 종사원들이 느끼는 건강저해인자에의 노출상태를 양호(A), 보통(B), 미흡(C), 불량(C)로 분류하고, 현장조사와 측정에 의한 각 인자의 노출상태를 양호(a), 보통(b), 미흡(c),불량(d)으로 분류하여 노출되고 있는 종사원의 분포를 검토한 결과는 다음과 같다. 1. 작업환경 측정결과 조도는 전체대상자 240명의 근로자 중 c등급 이하의 전반조도에 노출된 종사원은 86명(36%), c등급 이하의 국소조도에 노출된 종사자는 193명(80%)이었고, 온열조건에 관한 측정결과 c등급 이하의 환경에의 노출자가 34명(14%), c등급 이하의 소음환경에 180명(75%)이 노출되고 있었다. 공기 중의 먼지량과 톨루엔의 측정결과 c등급 이하의 환경에 노출된 사람은 없었으며, 외부의 자료와 노출상태를 조사한 결과 방사선과 항암제/항생제에 의한 c등급 이하의 환경에의 노출자도 없는 것으로 평가되었다. 2. 건강저해인자에 대하여 종사원이 느끼는 노출 정도(A, B, C, D등급)와 현장조사와 측정으로 확인된 노출 정도(a, b, c, d 등급)는 차이가 많아서, 특히 조도에서는 종사원이 저해환경을 과소평가하고, 공기 중 먼지에서는 과대평가가 심했으며, 온열조건, 소음, 방사선, 톨루엔, 항암제, 항생제에의 노출은 조사된 결과보다 나쁘게 느끼는 경향이 있었다. 3.설문에서 호소하는 소음의 증류로는 사람 말소리가 179명, 실내장비소리가 131명의 순이었으며, 실내공기 중 먼지의 종류로는 사람들의 옷먼지 108명, 건물먼지 79명의 순으로 많았다. 4. 설문에서 C등급 이하의 마취제에의 노출자가 28명, 알칼리/산에의 노출자가 10명, 약진에의 노출자가 6명이었으나 이들에 대한 측정이 불가능하여 평가할 수 없었다.

• 대한예방한의학회지
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• 제18권1호
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• pp.125-138
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• 2014

Objective : Polycan, exopolymers purified from Aureobasidium pullulans SM-2001 and calcium gluconate have been showed favorable inhibitory effects on the periodontitis and related alveolar bone losses through antioxidant and anti-inflammatory activities, respectively. In the present study, we intended to observe the possible synergic effects of mixed formula consisted of Polycan and calcium gluconate on ligation-induced experimental periodontitis and related alveolar bone losses in rats, and to select the fittest compositions for further developing as effective agents to ameliorate periodontal diseases. Method : Experiments were conducted as two separated two tests - first is synergic effects of Polycan and calcium gluconate 1:1, 1:9 and 9:1 mixtures, and second is 1:99, 2:98, 4:96, 8:92 and 1:9 mixtures. Experimental periodontal diseases were induced by ligature placed around the cervix of upper left incisior teeth of rats. One day after ligation placements, 200mg/kg of each single or mixed formulas of Polycan or/and calcium gluconate were orally administered for 10 days. The changes on the alveolar bone loss index and maxillary bone mineral density (BMD) were observed for detecting alveolar bone losses, and for anti-inflammatory effects, myeloperoxidase (MPO) activities and proinflammatory cytokine (tumor necrosis factor; TNF-${\alpha}$) contents were also evaluated in gingival tissues around ligature placed incisior teeth. The results of mixtures were compared with those of singe Polycan and calcium gluconate treated rat. Results : Each single or mixed formulas of Polycan or/and calcium gluconate favorably and significantly inhibited the inflammatory changes. The inhibitory effects of mixed formula consisted of Polycan and calcium gluconate 1:9 showed against periodontitis and related alveolar bone losses as compared with those of each Polycan and calcium gluconate single formula (p<0.05). In second experiment, Polycan and calcium gluconate 2:98, 4:96, 8:92 and 1:9 mixed formulas also showed significant increased anti-inflammatory and inhibitory effects against alveolar bone losses as compared with those of each single formula. Among them, Polycan and calcium gluconate 2:98 showed the highest efficacy against to ligation-induced experimental periodontitis and related alveolar bone losses. Conclusion : The results obtained in this study suggest that appropriated mixtures of Polycan and calcium gluconate showed synergic inhibitory effects against ligation-induced experimental periodontitis and related alveolar bone losses in rats. Moreover, Polycan and calcium gluconate 2:98 showed the highest efficacies in this experiment, suggesting the fittest composition for further developing as effective agents to ameliorate periodontal diseases.