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Effects of Kochiae Fructus Extracts on the Expression of Angiogeneis and Inflammation Related Proteins  

Na, Sang-Hyuk (Department of Preventive Medicine, College of Oriental Medicine, Kyunghee University)
Shin, Yong-Cheol (Department of Preventive Medicine, College of Oriental Medicine, Kyunghee University)
Ko, Seong-Gyu (Department of Preventive Medicine, College of Oriental Medicine, Kyunghee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.3, 2006 , pp. 557-562 More about this Journal
Abstract
Hypoxia induced angiogenesis and inflammation are essential processes for metastasis and progress of solid tumors. We examined the anti-angiogenic and inflammation related activity of Kochiae Fructus (KF) extract. To investigate the roles of the KF extract, we performed MTS assay, western blots using HaCaT cells and $HepG_2$ cells. The results are as follows. The protein level of $HIF-1{\alpha}$ was reduced when induced by $CoCl_2$ in $HepG_2$ cells treated with KF extract and induced by IGF-11 in HaCaT cells treated with KF extract. KF extract reduced the mRNA level of VEGF in HaCaT cells and KF extract reduced the protein level of iNOS in HaCaT cells. These results suggest that KF extract contributes to the anti-angiogenic and anti-inflammatory activities and also we could assume that KF extract act as antioxidant or anti-inflammatory agents via reduction of $HIF-1{\alpha}$.
Keywords
Kochiae Fructus (KF); $HIF-1{\alpha}$; iNOS; IGF-II; Angiogenesis;
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