• Molecular & Cellular Toxicology
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• 제4권1호
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• pp.66-71
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• 2008

Long term exposure of Jurkat cells to 2 ATA pressure resulted in the inhibition of cell growth. Under a 2 ATA pressure, the morphological changes in the cells were visualized by electron microscopy. The cells exhibited significant inhibitory responses after three passages. However, short-term exposure study was carried out, 2 ATA pressure may have beneficial effects. The Jurkat cells were exposed to $H_2O_2$ (25 and $50{\mu}M$) in order to induce DNA damage, and then incubated under at either normal pressure or 2 ATA for 1 or 2 hours in order to recover the DNA damage. The extent of DNA damage was determined via Comet assay. More recovery from DNA damage was observed at 2 ATA than at normal pressure. The activity of the DNA repair enzymes, DNA polymerase-$\beta$, was also evaluated at both normal pressure and 2 ATA. The activity of DNA polymerase-$\beta$ was observed to have increased significantly at the 2 ATA than at normal pressure. In conclusion, the effects of hyperbaric pressure from 1 ATA to 2 ATA on biochemical systems can be either beneficial or harmful. Long term exposure to hyperbaric pressure clearly inhibited cell proliferation and caused genotoxic effects, but short-term exposure to hyperbaric pressure proved to be beneficial in terms of bolstering the DNA repair system. The results of the present study have clinical therapeutic application, and might prove to be an useful tool in the study of genotoxicity in the future.

• 한국동물위생학회지
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• 제44권4호
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• pp.227-237
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• 2021

Artificial insemination of Korean native cattle (KNC) is the predominant method for breed improvement. However, industrialization of embryo production and transfer is necessary to utilize the genetic potential of KNC. The aim of this study was to examine associations between KNC donor cows and ovum pick-up (OPU) conditions, in-vivo oocyte recovery, and embryo development. Oocyte recovery and blastocyst development rates were higher at 50 and 60 mmHg OPU vacuum pressure than at 40 mmHg, which was, however, not significant. Regarding follicle growth, injection of 500 ㎍ GnRH 36 hours before OPU significantly increased the number of OPU oocytes from an average of 4.6 to 7.6 (P<0.05); no significant difference in embryo development rates was observed. Significant differences were observed in the numbers of OPU oocytes, embryo development rates, and transplantable blastocysts per individual among nine KNC donors (P<0.05). Furthermore, although there was no difference in OPU oocyte recovery intervals in approximately 2~8 weeks, the number of recovered oocytes significantly decreased at the 12-week interval (P<0.05); there was no difference in embryo development rates. The number of oocytes and embryonic development rates only tended to decrease until the seventh OPU session, but decreased significantly until the eighth session (P<0.05). The average pregnancy rate after transfer of OPU-derived in-vitro embryos into recipient cows was 41.8%. To improve the efficiency of OPU egg recovery and in-vitro embryo production, considering KNC donor characteristics, vacuum pressure of 60 mmHg, GnRH pretreatment to induce follicle growth, and effective OPU egg recovery up to seven times at intervals of 2~4 weeks appears to be most suitable. This study may facilitate the industrialization of KNC embryo production and transfer using high-quality cows.

• 공업화학
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• 제34권3호
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• pp.307-312
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• 2023

본 연구는 수소 경제 사회의 안전 확보를 위해 수소 연료전지 후단 배출된 미반응 수소를 안정적으로 산화하는 방안에 대해 논의하였다. 안전 시스템은 미반응 수소를 에너지원 없이 제거할 수 있는 상온 산화촉매를 충진하였으며, 이때 반응으로 배출되는 산화열은 안정적으로 회수할 수 있는 열 회수 장치를 연계하고자 하였다. 그 결과, 수소 산화 시스템의 충진 조건에 따라 시스템 내 압력 및 유체 흐름이 변화함을 CFD 분석을 통해 확인하였다. 또한 배가스 온도, 열 회수기 내 유량 및 압력조건을 최적화하여 300 ℃ 이상의 배가스 산화 열원을 40 ℃ 이상의 온수를 확보하는 방식으로 폐열을 회수할 수 있음을 확인하였다. 본 연구를 통해 수소 연료전지와 같은 중·소규모 사업장에 적용된 수소 활용 공정을 실증 규모로 평가하여 안전 시스템으로의 가능성을 확인하였다. 추후 실증화 연구를 통해 예측하지 못한 수소 안전사고에 대해 대응할 수 있는 안전 가이드로 활용될 수 있다고 판단된다.

• 대한기계학회논문집B
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• 제27권10호
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• pp.1412-1419
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• 2003

Heat regenerator occupied by regenerative materials improves thermal efficiency of combustion system through the recovery of sensible heat of exhaust gases. By using one-dimensional two-phase fluid dynamics model, the unsteady thermal flow of regenerator with spherical particles, was numerically analyzed to evaluate the heat transfer and pressure losses and to derive the design parameter for heat regenerator. It is confirmed that the computational results, such as air preheat temperature, exhausted gases outlet temperature, and pressure losses, agreed well with the experimental data. The thermal flow in heat regenerator varies with porosity, configuration of regenerator and diameter of regenerative particle. As the gas velocity increases with decreasing the cross-sectional area of the regenerator, the heat transfer between gas and particle enhances and pressure losses decrease. As particle diameter decreases, the air is preheated higher and the exhaust gases are cooled lower with the increase of pressure losses. Assuming a given exhaust gases temperature at the regenerator outlet, the regenerator need to be linearly lengthened with inlet Reynolds number of exhaust gases, which is defined as a regenerator design parameter.

• 설비공학논문집
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• 제14권12호
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• pp.1031-1038
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• 2002

The effects of casing shape on the performance and interaction between the impeller and casing in a small-size turbo-compressor are investigated. Numerical analysis is conducted for the compressor with circular and single volute casings from inlet to discharge nozzle. In order to predict the flow pattern inside the entire impeller, vaneless diffuer and casing, calculations with multiple frames of reference method between the rotating and stationery parts of the domain are carried out. For compressible turbulent flow fields, the continuity and three-dimensional time-averaged Wavier-Stokes equations are employed. To evaluate the performance of two types of casings, the static pressure and loss coefficients are obtained with various flow rates. Also, static pressure distributions around casings are studied for different casing shapes, which are very important to predict the distribution of radial load. To prove the accuracy of numerical results, measurements of static pressure around casing and pressure difference between the inlet and outlet of the compressor are peformed for the circular casing. Comparisons of these results between the experimental and numerical analyses are conducted, and reasonable agreement is obtained.

• 한국항공우주학회지
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• 제34권10호
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• pp.61-70
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• 2006

마하 4 비행 조건에서 작동하는 고성능 램제트와 듀얼모드 스크램제트 엔진의 초음속 공기 흡입구 모델을 설계하였다. 배압, 받음각, 요각 등 비행 변수의 변화에 따른 내부 유동 특성을 파악하기 위하여 규슈대학교의 불어내기식 초음속 풍동을 이용한 실험을 수행하였다. 유동 가시화를 위하여 쉴리렌 기법, 오일 가시화 기법을 이용하였으며, 정량적 성능 분석을 위하여 표면 압력 및 전압력을 측정하였다. 실험의 결과는 전산 유체 해석과 비교하였다. 본 연구는 기본적이지만 찾기 힘든 고 마하수 초음속 공기 흡입구 유동의 실험 결과를 제시한다.

• 상하수도학회지
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• 제22권2호
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• pp.267-274
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• 2008

This study is about efficiency of pretreatment process and operating factor to membrane process at continuous coagulation/ultrafiltration process in water treatment. The capacity of pilot plant was $0.06{\beta}(C)/d$. The raw water used was from Nakdong stream which was characteristized by high organic matter and high turbidity. The result of the test was that coagulation is good process as to high removal rate to organic matter and turbidity but It caused problem to membrane pore blocking. This paper is to determine the membrane fouling potential under different membrane flux, backwash pressure and linear velocity. Backwash pressure and flux is important parameter on operation of membrane system. Those are directly affected on membrane system. When backwash pressure increased from 150 kPa to 200 kPa, the result showed that fouling (pressure increase rate) changed from 3.69 kPa/h to 0.93 kPa/h and the recovery rate changed from 90.7 % to 82.0 %. Linear velocity had slightly effect on fouling. Linear velocity increased from 0.2 m/s to 0.5 m/s, the corresponding pressure rate changed from 0.93 kPa/d to 0.77 kPa/d.

• 한국유체기계학회 논문집
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• 제17권6호
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• pp.13-20
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• 2014

It is necessary to design a compressor with high pressure ratio that satisfies the IMO(international maritime organization) NOx emission regulation for the marine diesel engine. Impeller was designed using the modified slip factor with the flow coefficient. The main purpose of this study is to investigate the sensitivity of the compressor performance by the vaned diffuser geometries. The first vaned diffuser type was based on a NACA airfoil, the second was channel diffuser, and the third was conformally transformated configuration of a NACA65(4A10)06 airfoil. The sensitivity of the performance was calculated using a commercial CFD program for three different diffuser geometries. The channel diffuser showed the wide range of operation and higher pressure characteristics, comparing with the others. This is attributed to the flow stability at diffuser. Combined with this results with impeller design, the optimized compressor was designed and verified by the test results.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2010년도 제34회 춘계학술대회논문집
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• pp.321-325
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• 2010

Supersonic ejectors are simple mechanical components, which generally perform mixing and/or recompression of two fluid streams. Ejectors have found many applications in engineering. In aerospace engineering, they are used for altitude testing of a propulsion system by reducing the pressure of a test chamber. It is composed of three major sections: a vacuum test chamber, a propulsive nozzle, and a supersonic exhaust diffuser. This paper aims at the improvement of ejector-diffuser performance by focusing attention on reducing exhaust back flow into the test chamber, since alteration of the backflow or recirculation pattern appears as one of the potential means of significantly improving low supersonic ejector-diffuser performance. The simplest backflow-reduction device was an orifice plate at the duct inlet, which would pass the jet and entrained fluid but impede the movement of fluid upstream along the wall. Results clearly showed that the performance of ejector-diffuser system was improved for certain a range of system pressure ratios, whereas the orifice plate was detrimental to the ejector performance for higher pressure ratios. It is also found that there is no change in the performance of diffuser with orifice at its inlet, in terms of its pressure recovery. Hence an appropriately sized orifice system should produce considerable improvement in the ejector-diffuser performance in the intended range of pressure ratios.

• 한국자동차공학회논문집
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• 제3권5호
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• pp.47-55
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• 1995

Cylindrical chokes are used widely as components of hydraulic equipments. The dynamic charac teristics between flowrate and pressure drop through the cylindrical chokes were discussed by the frequency characteristics of the chokes. It was assumed no pressure recovery occured at the downstream neighborhood of the choke. The pulsating jetflow from outlet of cylindrical chokes shows very complex behaviours which are quite different from the steady jetflow but it is not clarified quantitatively. In order to utilize the chokes as a flowmeter, it is indispensable to discuss the estimation of the dynamics of pressure drop in the downstream jetflow region of cylindrical chokes. In this experimental study, the dynamic behaviours of the jetflow in the downstream region of cylindrical chokes are investigated precisely by using flow visualization. In the results of experimental sutdy, it is clarified that the retachment length depended on pressure wave is compared with it depended on velocity wave.