Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Effects of donors and in vivo ovum pick-up conditions on in vitro embryo development in Korean native cow

한우 공란우 및 생체내 난자 회수(ovum pick-up) 조건이 체외수정란의 발달에 미치는 효과

  • Park, Yong Soo (Department of Horse Industry, Korea National College of Agriculture and Fisheries,) ;
  • Kong, Jun Ho (Department of Animal Resources Technology, Gyeongsang National University) ;
  • Yi, Jun Koo (Gyeongsangbuk-Do Livestock Research institute) ;
  • Oh, Dong yep (Gyeongsangbuk-Do Livestock Research institute) ;
  • Chung, Ki Hwa (Department of Animal Resources Technology, Gyeongsang National University)
  • 박용수 (국립한국농수산대학 말산업학과) ;
  • 공준호 (경상국립대학교 동물소재공학과) ;
  • 이준구 (경상북도축산기술연구소) ;
  • 오동엽 (경상북도축산기술연구소) ;
  • 정기화 (경상국립대학교 동물소재공학과)
  • Received : 2021.11.11
  • Accepted : 2021.12.10
  • Published : 2021.12.30
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Abstract

Artificial insemination of Korean native cattle (KNC) is the predominant method for breed improvement. However, industrialization of embryo production and transfer is necessary to utilize the genetic potential of KNC. The aim of this study was to examine associations between KNC donor cows and ovum pick-up (OPU) conditions, in-vivo oocyte recovery, and embryo development. Oocyte recovery and blastocyst development rates were higher at 50 and 60 mmHg OPU vacuum pressure than at 40 mmHg, which was, however, not significant. Regarding follicle growth, injection of 500 ㎍ GnRH 36 hours before OPU significantly increased the number of OPU oocytes from an average of 4.6 to 7.6 (P<0.05); no significant difference in embryo development rates was observed. Significant differences were observed in the numbers of OPU oocytes, embryo development rates, and transplantable blastocysts per individual among nine KNC donors (P<0.05). Furthermore, although there was no difference in OPU oocyte recovery intervals in approximately 2~8 weeks, the number of recovered oocytes significantly decreased at the 12-week interval (P<0.05); there was no difference in embryo development rates. The number of oocytes and embryonic development rates only tended to decrease until the seventh OPU session, but decreased significantly until the eighth session (P<0.05). The average pregnancy rate after transfer of OPU-derived in-vitro embryos into recipient cows was 41.8%. To improve the efficiency of OPU egg recovery and in-vitro embryo production, considering KNC donor characteristics, vacuum pressure of 60 mmHg, GnRH pretreatment to induce follicle growth, and effective OPU egg recovery up to seven times at intervals of 2~4 weeks appears to be most suitable. This study may facilitate the industrialization of KNC embryo production and transfer using high-quality cows.

Keywords

Acknowledgement

이 논문은 2020-2021년도 경상국립대학교 대학회계 연구비 지원에 의하여 연구되었음.

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