Browse > Article
http://dx.doi.org/10.7853/kjvs.2021.44.4.227

Effects of donors and in vivo ovum pick-up conditions on in vitro embryo development in Korean native cow  

Park, Yong Soo (Department of Horse Industry, Korea National College of Agriculture and Fisheries,)
Kong, Jun Ho (Department of Animal Resources Technology, Gyeongsang National University)
Yi, Jun Koo (Gyeongsangbuk-Do Livestock Research institute)
Oh, Dong yep (Gyeongsangbuk-Do Livestock Research institute)
Chung, Ki Hwa (Department of Animal Resources Technology, Gyeongsang National University)
Publication Information
Korean Journal of Veterinary Service / v.44, no.4, 2021 , pp. 227-237 More about this Journal
Abstract
Artificial insemination of Korean native cattle (KNC) is the predominant method for breed improvement. However, industrialization of embryo production and transfer is necessary to utilize the genetic potential of KNC. The aim of this study was to examine associations between KNC donor cows and ovum pick-up (OPU) conditions, in-vivo oocyte recovery, and embryo development. Oocyte recovery and blastocyst development rates were higher at 50 and 60 mmHg OPU vacuum pressure than at 40 mmHg, which was, however, not significant. Regarding follicle growth, injection of 500 ㎍ GnRH 36 hours before OPU significantly increased the number of OPU oocytes from an average of 4.6 to 7.6 (P<0.05); no significant difference in embryo development rates was observed. Significant differences were observed in the numbers of OPU oocytes, embryo development rates, and transplantable blastocysts per individual among nine KNC donors (P<0.05). Furthermore, although there was no difference in OPU oocyte recovery intervals in approximately 2~8 weeks, the number of recovered oocytes significantly decreased at the 12-week interval (P<0.05); there was no difference in embryo development rates. The number of oocytes and embryonic development rates only tended to decrease until the seventh OPU session, but decreased significantly until the eighth session (P<0.05). The average pregnancy rate after transfer of OPU-derived in-vitro embryos into recipient cows was 41.8%. To improve the efficiency of OPU egg recovery and in-vitro embryo production, considering KNC donor characteristics, vacuum pressure of 60 mmHg, GnRH pretreatment to induce follicle growth, and effective OPU egg recovery up to seven times at intervals of 2~4 weeks appears to be most suitable. This study may facilitate the industrialization of KNC embryo production and transfer using high-quality cows.
Keywords
Korean native cattle; Ovum pick-up; In vitro fertilization; Embryo;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Rivera FA, Mendonca LGD, Lopes Jr. G, Santos JEP, Perz RV, Amstalden M, Correa-Calderon A, Chebel C. 2011. Reduced progesterone concentration during growth of the first follicular wave affects embryo quality but has no effect on embryo survival post transfer in lactating dairy cows. Reproduction 141: 333-342.   DOI
2 Santos JEP, Cerri RLA, Sartori R. 2008. Nutritional management of the donor cow. Theriogenology 69: 88-97.   DOI
3 Staigmiller RB, Short RE, Bellows RA, Carr JB. 1979. Effect of nutrition on response to exogenous FSH in beef cattle. J. Anim. Sci. 48: 1182-1190.   DOI
4 Van Wagtendonk-de Leeuw AM, Mullaart E, Roos de APW, Merton JS, Den Daas JHG and de Ruigh L. 2000. Effects of different reproduction techniques: AI, MOET or IVP, on health and welfare of bovine offspring. Theriogenology 53: 575-597.   DOI
5 농림축산식품부. 2020. 정액증명서등. 축산법 제18조.
6 Hasler JF. 2001. Factors affecting frozen and fresh embryo transfer pregnancy rates in cattle. Theriogenology 56: 1401-1415.   DOI
7 Hawk HW, Wall RJ. 1995. Improved yield of bovine blastocysts from in vitro produced oocytes selection of oocytes and zygotes. Theriogenology 41: 1571-1583.   DOI
8 Hendriksen PJM, Vos PLAM, Steenweg WNM, Bevers MM, Dieleman SJ. 2000. Bovine follicular development and its effect on the in vitro competence of oocytes. Theriogenology 53: 11-20.   DOI
9 Jin JI, Choi BH, Kim SS, Park BY, Lee JG, Kong IL. 2015. Possibility of repeated use of elite cows for mass production of OPU-derived embryos. J. Emb. Trans. 30: 149-159.   DOI
10 Jin JI, Kwon TH, Choi BH, Kim SS, Jo HT, Kong IK. 2010. Effect of OPU(ovum pick-up) duration on the rate of collected ova and in vitro produced blastocyst formation. J. Emb. Trans. 25: 15-20.
11 Leroy JL, Opsomer G, Van Soom A, Goovaerts IG, Bols PE. 2008a. Reduced fertility in high-yielding dairy cows: are the oocyte and embryo in danger? Part I. The importance of negative energy balance and altered corpus luteum function to the reduction of oocyte and embryo quality in high-yielding dairy cows. Reprod. Domest. Anim. 43: 612-622.   DOI
12 Lonergan P, Fair T, Corcoran D and Evans ACO. 2006. Effect of culture environment on gene expression and develop mental characteristics in IVF-derived embryos. Theriogenology 65: 137-152.   DOI
13 Matoba S, Yoshioka H, Matsuda H, Sugimura S, Aikawa Y, Ohtake M, Hashiyada Y, Seta T, Nakagawa K, Lonergan P, Imai K. 2014. Optimizing production of in vivo-matured oocytes from superstimulatedHolstein cows for in vitro production of embryos using X-sorted sperm. J. Dairy Sci. 97: 743-753.   DOI
14 Pieterse MC, Kappen KA, Kruop ThAM, Taverne NAM. 1988. Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries. Theriogenology 30: 751-762.   DOI
15 Merton JS, Ask B, Onkundi DC, Mullaart E, Colenbrander B, Nielen M. 2009. Genetic parameters for oocyte number and embryo production within a bovine ovum pick-up-in vitro production embryo-production program. Theriogenology 72: 885-893.   DOI
16 Nasser LF, Sa Filho MF, Reis EL, Rezende CR, Mapletoft RJ, Bo GA, Baruselli PS. 2011. Exogenous progesterone enhances ova and embryo quality following superstimulation of the first follicular wave in Nelore (Bos indicus) donors. Theriogenology 76: 320-327.   DOI
17 Nolan R, O'Callaghan D, Duby RT, Lonergan P, Boland MP, 1998. The influence of short-term nutrient changes on follicle growth and embryo production following superovulation in beef heifers. Theriogenology 50: 1263-1274.   DOI
18 Leroy JL, Rizos D, Sturmey R, Bossaert P, Gutierrez-Adan A, Van Hoeck V, Valckx S, Bols PEJ. 2011. Intrafollicular conditions as a major link between maternal metabolism and oocyte quality: a focus on dairy cow fertility. Reprod. Fertil. Dev. 24: 1-12.   DOI
19 Park SJ, Yang S, Im KS, Sang HH, Chang WK, Cheong IC, Park CS. 2000a. The study of factor concentrating ovum pick-up and conception rate by ultrasound-guided follicular aspiration in hanwoo heifers. Korean J. Animal Reprod. 24: 199-208.
20 Park SJ, Yang BS, Im GS, Seong HH, Chang WK, Park CS. 2000b. Studies on the ultrasound-guided transvaginal retrieval of oocytes in korean native, hanwoo heifers I. Characteristics of hanwoo ovary during the estrous cycle. Korean J. Animal. Reprod. 24: 77-82.
21 Pursely JR, Martins JP. 2011. Impact of circulating concentrations of progesterone and antral age of the ovulatory follicle on fertility of high-producing lactating dairy cows. Reprod. Fertil. Dev. 24: 267-271.   DOI
22 Rizos D, Ward F, Duffy P, Boland MP, Lonergan P. 2002. Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro verses in vivo: Implications for blastocyst yield and blastocyst quality. Mol. Reprod. Dev. 61: 234-248.   DOI
23 Blondin P, Sirard MA. 1995. Oocyte and follicular morphology as determining characteristics for developmental competence in bovine oocytes. Mol. Reprod. Dev. 41: 54-62.   DOI
24 De Roover R, Feugang JM, Bols PE, Genicot G, Hanzen C. 2008. Effects of ovum pick-up frequency and FSH stimulation: a retrospective study on seven years of beef cattle in vitro embryo production. Reprod. Domest. Anim. 43: 239-45.   DOI
25 Sakaguchi K, Maylem ERS, Tilwani RC, Yanagawa Y, Katagiri S, Atabay EC, Atabay EP, Nagano M. 2019. Effects of follicle-stimulating hormone followed by gonadotropin-releasing hormone on embryo production by ovum pick-up and in vitro fertilization in the river buffalo(Bubalus bubalis). Anim. Sci. J. 90: 690-695.   DOI
26 Scott CA, Robertson L, de Moura RTD, Patterson C, Boyd JS. 1994. Technical aspects of transvaginal ultrasound-guided follicullar aspiration in cows. Vet. Rec. 134: 440-443.   DOI
27 Snijders SEM, Dillon P, O'Callaghan D, Boland MP. 2000. Effect of genetic merit, milk yield, body condition and lactation number on in vitro oocyte development in dairy cows. Theriogenology 53: 981-989.   DOI
28 Machatkova M, Krausova K, Jokesova E, Tomanek M. 2004. Developmental competence of bovine oocytes: effects of follicle size and the phase of follicular wave on in vitro embryo production. Theriogenology 61: 329-335.   DOI
29 Su L, Yang S, He X, Li X, Ma J, Wang Y, Presicce GA. W JI. 2012. Effect of donor age on the developmental competence of bovine ooeytes retrievd by ovum pick up. Reprod. Dom. Anim. 47: 184-189.   DOI
30 Sunderland SJ, Crowe MA, Boland MP, Roche JF, Ireland JJ. 1994. Selection, dominance and atresia of follicles during the oestrous cycle of heifers. J. Reprod. Fertil. 101: 547-555.   DOI
31 Bols PEJ, Ysebaert MT, Van Soom A, de Kruif A. 1997. Effect of needle tip bevel and aspiration procedure on the morphology and developmental capacity of bovine compact cumulus oocytes complexes. Theriogenology 46: 899-909.   DOI
32 Ward FA, Lonergan P, Enright BP, Boland MP. 2000. Factors affecting recovery and quality of oocytes for bovine embryo production in vitro using ovum pick-up technology. Theriogenology 54: 433-446.   DOI
33 Yaakub H, O'Callaghan D, Boland MP, 1999. Effect of type and quantity of concentrates on superovulation and embryo yield in beef heifers. Theriogenology 51: 1259-1266.   DOI
34 Hasler JF, Henderson WB, Hurtgen PJ, Jin ZQ, McCauley AD, Mower SA, Neely B, Shuey LS, Stokes JE, Trimmer SA. 1995. Production, freezing and transfer of bovine IVF embryos and subsequent calving results. Theriogenology 43: 141-152.   DOI
35 Chaubal SA, Ferre LB, Molina JA, Faber DC, Bols PJ, Rezamand PTX, Yang X. 2007. Hormonal treatments for increasing the oocyte and embryo production in an OPU-IVP system. Theriogenology 67: 719-728.   DOI
36 Chaubal SA, Molina JA, Ohlrichs CL, Ferre LB, Faber DV, Bols PEJ, Riesen JW, Tian X, Yang X. 2006. Comparison of different transvaginal ovum pick-up protocols to optimise oocyte retrieval and embryo production over at 10-week period in cows. Theriogenology 65: 1631-1648.   DOI
37 De Roover R, Genicot G, Leonard S, Bols P, Dessy F. 2005. Ovum pick up and in vitro embryo production in cows superstimulated with an individually adapted superstimulation protocol. Ani. Reprod. Sci. 86: 13-25.   DOI
38 Galli C, Duchi R, Colleoni S, Lagutina I, Lazzari G. 2014. Ovum pick up, intracytoplasmic sperm injection and somatic cell nuclear transfer in cattle, buffalo and horses: from the research laboratory to clinical practice. Theriogenology 81: 138-151.   DOI
39 Jin JI, Choi BH, Kim SS, Jo HT, Sun DW, Lim HT, Lee JG, Min CS, Kong IL. 2014. Transplantation and production of OPU derived hanwoo IVP embryos. J. Emb. Trans. 29: 273-281.   DOI
40 Galli C, Crotti G, Notari C, Turini P, Duchi R, Lazzari G. 2001. Embryo production by ovum pick up from live donors. Theriogenology 55: 1341-57.   DOI
41 Chastant-Maillard S, Quinton H, Lauffenburger J, Cordonnier-Lefort N, Richard C, Marchal J, Mormede P, Renard JP. 2003. Consequences of transvaginal follicular puncture on well-being in cows. Reproduction 125: 555-63.   DOI
42 Cho SR, Kang SK, Kim UH, Lee SD, Lee MS, Yang BC. 2017. Study on ovum-pick up for improvement of embryo transfer efficiency in hanwoo cows. J. Emb. Trans. 32: 147-151.   DOI
43 Numabe T, Oikawa T, Kikuchi T, Horiuchi T, 2000. Production efficiency of Japanese black calves by transfer of bovine embryos produced in vitro. Theriogenology 54: 1409-1420.   DOI
44 Leroy JL, Vanholder MR, Mateusen T, Christophe B, Opsomer A, de Kruif G, Genicot A, Van Soom A. 2005. Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro. Reproduction 130: 485-495.   DOI
45 Demetrio D, Looney C, Rees H, Werhman M. 2020. Statistical information committee report (2019 DATA). Annual Report of the AETA Statistical Information Committee. (https://www.aeta.org/docs/2019_Stats.pdf)
46 Galli C, Grotti G, Notari C, Turini P, Duchi R, Lazzari G. 2000. Embryo production by ovum pick up from live donors. Theriogenology 55: 1341-1357.   DOI
47 Hasler JF. 2014. Forty years of embryo transfer in cattle: A review focusing on the journal Theriogenology, the growth of the industry in North America, and personal reminisces. Theriogenology 81: 152-169.   DOI
48 Kim SS, Park YS. 2011. Associations between pedigree, meat quality, and meat quantity of slaughterhouse donor cows and oocyte recovery, embryo development, and pregnancy after embryo transfer. Livestock Science 142: 42-47.   DOI
49 Leroy JL, Van Soom A, Opsomer G, Goovaerts IG, Bols PE. 2008b. Reduced fertility in high-yielding dairy cows: are the oocyte and embryo in danger? Part II. Mechanisms linking nutrition and reduced oocyte and embryo quality in high-yielding dairy cows. Reprod. Domest. Anim. 43: 623-632.   DOI
50 Merton JS, de Roos AP, Mullaart E, de Ruigh L, Kaal L, Vos PL, Dieleman SJ. 2003. Factors affecting oocyte quality and quantity in commercial application of embryo technologies in the cattle breeding industry. Theriogenology 59: 651-674.   DOI
51 Park SJ, Yang BS, Im GS, Seong HH, Chang WK, Park CS. 2000c. Studies on the ultrasound-guided transvaginal retrieval of oocytes in korean native, hanwoo heifers II. Effects of repeated ovum pick-up on oocyte recovery and estrous cycle. Korean J. Animal. Reprod. 24: 83-88.