• 한국동물위생학회지
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• 제36권4호
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• pp.291-296
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• 2013

The purpose of this study was to determine food safety practices and procedures based on Hazard Analysis Critical Control Point (HACCP) system and to suggest more effective method of HACCP implementation in meat packaging industry in Korea. We used the non-compliance rate of each evaluation item to compare the weak points of prerequisite requirements and HACCP. The prerequisite items related to facility, equipment, and tools showed inadequate level of requirements or unsanitary conditions for proper HACCP operation. A lack of understanding of sanitation standard operation procedures was identified as a fundamental barrier to HACCP implementation. High rate of non-compliance in HACCP items compared to prerequisite requirements signify that small businesses have potential difficulties of applying HACCP due to lack of technical expertise, financial resources for prerequisite requirements, and available personnel to prepare and operate HACCP plan. Also we suggest to revise and minimize current critical control points (CCPs). Time-temperature control of cold-storage rooms for carcasses and final products could be performed by control points of prerequisite requirements. As the occurrence frequency getting lower, metal detector should be replaced by intensified training of sanitary handling and safety procedure. This will be more effective and preventive measures against physical contaminants including metal particles. In conclusion, control point of prerequisite requirement may replace CCP in the plant with simple processing line and no heating process such as meat packaging industry.

• 한국군사과학기술학회지
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• 제12권3호
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• pp.290-298
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• 2009

This paper proposes a novel process which can develop the system requirements in defense R&D programs. As the weapon systems become more complicated, the success and effectiveness of R&D outcome heavily depend on the application and tailoring of systems engineering process and methods. And, the accuracy and quality of system requirements are essential prerequisite to leverage the systems engineering process. To produce the artifacts of systems engineering such as OCD, ORD, and the systems requirements, the system user can write out requirement document using the proposed implementation process and templates without expending heavy work loads.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2005년도 춘계학술대회
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• pp.252-255
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• 2005

The effort to realize the concept of stratospheric airship, which can fly at about 20km altitude, has been persevered since late 1980's. Referring to the feasibility study of ensuring the flight duration of the airship over 1year, total weight is about 30 tons, the length is about 200m. There are lots of key technologies to be solved to develop the system, and one of the essential prerequisite technologies is regenerative fuel cell system. In this paper, design requirement of regenerative fuel cell system is introduced with the feasibility study results of the challenging stratospheric airship.

• 공학교육연구
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• 제14권4호
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• pp.48-61
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• 2011

국내외 기계공학분야 교육과정과 이수체계에 대한 고찰을 통하여 교육과정 및 이수체계 현황을 파악하고, 공학교육 내용과 지식의 논리적 구조에 근거한 합리적 이수체계 모델을 제시하여, 기계공학 프로그램의 운영 방향을 제시하고자 한다. 국내외 우수대학 기계공학 분야의 설계교육을 포함한 교육과정, 학문, 산업발전 동향을 고려하여 합리적 이수체계를 연구하였다. 특히 전면적인 선.후수 이수체계도입을 위한 방법을 연구하여, 교과 이수체계를 준수할 수 있는 제도적 방안을 제시함으로써 학생들이 공학지식의 논리적 구조에 따라 공학 관련 지식을 체계적으로 습득할 수 있도록 하고자 한다.

• 대한안전경영과학회:학술대회논문집
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• 대한안전경영과학회 2001년도 춘계학술대회
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• pp.275-280
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• 2001

DESIGNIN AND OPERATION OF DIGITAL EVIDENCE MANAGEMENT SYSTEM APPLYING COMPUTER FORENSICS AND ELECTRONIC CERTIFICATION Digital evidence will be used as a term, which means the electronic form of information which is necessary to confirm or prove the factum of all kinds of behaviors committed through the devices which have data processing ability including computer. It is expected that there will be the increase of legal conflicts surrounding electronic commerce activities as well as the increase of cyber crimes, as the number of Internet users are getting bigger. In order to solve the problems of conflicts derived from electronic commerce, the factum of electronic commerce activities must be confirmed. In order to confirm the factum of electronic commerce activities, the evidence is prerequisite. Almost all evidences relating to the electronic commerce activities exist in digital form. For the reason that the digital evidence can be easily damaged and changed, special management is required to collect, analyze, and preserve the digital evidence. In order to meet this requirement, this study proposes a basic model of digital evidence management system applying computer forensics and electronic authentication.

• Journal of information and communication convergence engineering
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• 제11권4호
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• pp.223-228
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• 2013

Cognitive radio has been recently considered a promising technology to improve spectrum utilization by enabling secondary access to licensed bands that are not used by primary users temporarily or spatially. A prerequisite to this secondary access is the lack of interference to the primary system. This requirement makes spectrum sensing a key process for cognitive radio. In this study, we consider amplify and forward (AF)-based cooperative spectrum sensing for cognitive radio networks where multiple relay nodes are utilized to amplify and forward the primary user signal for better spectrum sensing, and maximum ratio combining is used for fusion detection by a cognitive coordinator. Further, the detection probability and the bit error rate of AF-based cooperative spectrum sensing are analyzed in fading multiple cognitive relay channels. The simulation results show that the AF-based cooperative spectrum sensing scheme outperforms the conventional scheme.

• 한국음향학회지
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• 제30권8호
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• pp.461-467
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• 2011

제한된 대역폭을 효율적으로 사용하기 위해서 도입된 가변 전송률은 먼저 신호의 정확한 분류를 필요로 한다. 특히 멀티미디어 서비스가 보편화 되면서 음성/음악 신호 분류의 중요성도 높아지게 되었다. 음성/음악 분류기 중, 서포트벡터머신 (SVM)을 이용한 분류기는 높은 분류 정확도로 주목받고 있다. 그러나 SVM는 많은 계산량과 저장 공간을 요구하므로 효율적인 구현이 요구되며, 특히 임베디드 시스템과 같이 자원이 제한 적인 경우에는 더욱 그러하다. 본 논문에서는 먼저 SVM을 이용한 음성/음악 분류기의 임베디드 시스템으로의 구현을 실행시간과 에너지소비의 관점에서 분석하고, 효율적인 구현을 위한 두가지 방법들을 제안한다. 서포트벡터의 판별결과에의 기여도를 바탕으로 기여도가 낮은 벡터들을 제외하는 방법과, 음성/음악 신호에 기본적으로 존재하는 각 프레임간의 상관관계를 이용하여 입력신호의 일부를 건너뛰는 방법이다. 이 기법들은 SVM의 학습 시 사용되는 다른 최적화 기법에 관계없이 적용이 가능하며, 실험을 통해 분류의 정확도, 실행시간, 그리고 에너지소비의 관점에서 그 성능을 증명하였다.

• Applied Microscopy
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• 제32권4호
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• pp.311-317
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• 2002

입자 크기에 따라 상전이 경로가 달라지며 매우 큰 응집체로 존재하는 gibbsite 분말과 같은 재료의 정량적인 전이 구조 분석의 전제로서 입자 크기가 선택적인 TEM 시편의 준비가 절실히 요구된다. 입자 크기 선택성을 실현한 TEM 시편 준비의 방법론을 확립하기 위한 방안으로서 본 실험이 전개되었다. 약 1 wt%의 gibbsite 원료 분말을 에탄올 용매에 혼합 한 뒤, ball-milling에 의해 조분쇄 처리를 하고 증류수에 1 : 19의 비로 희석된 분산 조제, Darvan C, 0.25 vol%의 첨가와 함께 ultrasonic 처리를 하여 gibbsite suspension을 만들었다. 제조된 gibbsite suspension의 누적 농도 변화 관찰에 의한 침강 시간의 조정 후 정적 침강 분리에 의해 nm 크기별로 분리된 TEM 시편을 만들 수가 있었다. 시편의 전체적인 양상은 SEM으로 관찰하였고 개개 입자들의 morphology 는 TEM으로 분석하였다. 또한, 공정 과정 중의 상변화 가능성을 검토하기 위해 XRD 분석을 실시하였다.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.101-111
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• 2017

The task of improving a fungal strain is highly time-consuming due to the requirement of a large number of flasks in order to obtain a library with enough diversity. In addition, fermentations (particularly those for fungal cells) are typically performed in high-volume (100-250 ml) shake-flasks. In this study, for large and rapid screening of itaconic acid (IA) high-yielding mutants of Aspergillus terreus, a miniaturized culture method was developed using 12-well and 24-well microtiter plates (MTPs, working volume = 1-2 ml). These miniaturized MTP fermentations were successful, only when highly filamentous forms were induced in the growth cultures. Under these conditions, loose-pelleted morphologies of optimum sizes (less than 0.5 mm in diameter) were casually induced in the MTP production cultures, which turned out to be the prerequisite for the active IA biosynthesis by the mutated strains in the miniaturized fermentations. Another crucial factor for successful MTP fermentation was to supply an optimal amount of dissolved oxygen into the fermentation broth through increasing the agitation speed (240 rpm) and reducing the working volume (1 ml) of each 24-well microtiter plate. Notably, almost identical fermentation physiologies resulted in the 250 ml shake-flasks, as well as in the 12-well and 24-well MTP cultures conducted under the respective optimum conditions, as expressed in terms of the distribution of IA productivity of each mutant. These results reveal that MTP cultures could be considered as viable alternatives for the labor-intensive shake-flask fermentations even for filamentous fungal cells, leading to the rapid development of IA high-yield mutant strains.

• Fisheries and Aquatic Sciences
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• 제19권4호
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• pp.21.1-21.11
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• 2016

Background: The evaluation of suitable reference genes as normalization controls is a prerequisite requirement for launching quantitative reverse transcription-PCR (RT-qPCR)-based expression study. In order to select the stable reference genes in abalone Haliotis discus hannai tissues (gill and hepatopancreas) under heavy metal exposure conditions (Cu, Zn, and Cd), 12 potential candidate housekeeping genes were subjected to expression stability based on the comprehensive ranking while integrating four different statistical algorithms (geNorm, NormFinder, BestKeeper, and ${\Delta}CT$ method). Results: Expression stability in the gill subset was determined as RPL7 > RPL8 > ACTB > RPL3 > PPIB > RPL7A > EF1A > RPL4 > GAPDH > RPL5 > UBE2 > B-TU. On the other hand, the ranking in the subset for hepatopancreas was RPL7 > RPL3 > RPL8 > ACTB > RPL4 > EF1A > RPL5 > RPL7A > B-TU > UBE2 > PPIB > GAPDH. The pairwise variation assessed by the geNorm program indicates that two reference genes could be sufficient for accurate normalization in both gill and hepatopancreas subsets. Overall, both gill and hepatopancreas subsets recommended ribosomal protein genes (particularly RPL7) as stable references, whereas traditional housekeepers such as ${\beta}-tubulin$ (B-TU) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes were ranked as unstable genes. The validation of reference gene selection was confirmed with the quantitative assay of MT transcripts. Conclusions: The present analysis showed the importance of validating reference genes with multiple algorithmic approaches to select genes that are truly stable. Our results indicate that expression stability of a given reference gene could not always have consensus across tissue types. The data from this study could be a good guide for the future design of RT-qPCR studies with respect to metal regulation/detoxification and other related physiologies in this abalone species.