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http://dx.doi.org/10.4014/jmb.1610.10037

Development of Miniaturized Culture Systems for Large Screening of Mycelial Fungal Cells of Aspergillus terreus Producing Itaconic Acid  

Shin, Woo-Shik (Green Materials and Process R&BD Group, Korea Institute of Industrial Technology)
Lee, Dohoon (Green Materials and Process R&BD Group, Korea Institute of Industrial Technology)
Kim, Sangyong (Green Materials and Process R&BD Group, Korea Institute of Industrial Technology)
Jeong, Yong-Seob (Department of Food Science and Technology, Chonbuk National University)
Chun, Gie-Taek (College of Biomedical Science, Kangwon National University)
Publication Information
Journal of Microbiology and Biotechnology / v.27, no.1, 2017 , pp. 101-111 More about this Journal
Abstract
The task of improving a fungal strain is highly time-consuming due to the requirement of a large number of flasks in order to obtain a library with enough diversity. In addition, fermentations (particularly those for fungal cells) are typically performed in high-volume (100-250 ml) shake-flasks. In this study, for large and rapid screening of itaconic acid (IA) high-yielding mutants of Aspergillus terreus, a miniaturized culture method was developed using 12-well and 24-well microtiter plates (MTPs, working volume = 1-2 ml). These miniaturized MTP fermentations were successful, only when highly filamentous forms were induced in the growth cultures. Under these conditions, loose-pelleted morphologies of optimum sizes (less than 0.5 mm in diameter) were casually induced in the MTP production cultures, which turned out to be the prerequisite for the active IA biosynthesis by the mutated strains in the miniaturized fermentations. Another crucial factor for successful MTP fermentation was to supply an optimal amount of dissolved oxygen into the fermentation broth through increasing the agitation speed (240 rpm) and reducing the working volume (1 ml) of each 24-well microtiter plate. Notably, almost identical fermentation physiologies resulted in the 250 ml shake-flasks, as well as in the 12-well and 24-well MTP cultures conducted under the respective optimum conditions, as expressed in terms of the distribution of IA productivity of each mutant. These results reveal that MTP cultures could be considered as viable alternatives for the labor-intensive shake-flask fermentations even for filamentous fungal cells, leading to the rapid development of IA high-yield mutant strains.
Keywords
Itaconic acid; Aspergillus terreus; strain improvement; morphology; microtiter plate; miniature culture;
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