• Reproductive and Developmental Biology
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• v.34 no.3
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• pp.161-167
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• 2010

Early diagnoses of pregnancy for animal such as swine and bovine is extremely important to increase income of a farmhouse and for the management of farm. For the development of immunoasaay system of pregnancy in swine, we report a competitive heterologous enzyme linked immunosorbent assay (ELISA) for the direct measurement of oestrone sulfate (E1S) in diluted urine using anti-E1G (glucuronide) monoclonal antibody which cross react with ElS. The principle of assay was based on the typical solid-phase competitive ELISA methods using E1G-HRP (horseradish peroxidase) as a tracer and E1S for standard. The method had a reasonable sensitivity for the detection of E1S with 0.15 ng/ml as a detection limit. The intra-assay and inter-assay precisions were raging coefficient of from 8.50~9.67% and 8.50~9.87%, respectively, which were quite acceptable. In a field trial with a group 37 sows (18 non-pregnancy and 19 pregnancy sows) after day 29~30 post service, the concentration of E1S were determined to be below 30 ng/ml in all non-pregnancy group and over 48 ng/ml in pregnancy group except one sample. The method described here, heterologous ELISA for the measurement of E1S in urine is good enough for monitoring the early pregnancy test of swine.

• Korean Parent-Child Health Journal
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• v.9 no.2
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• pp.101-115
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• 2006

Purpose: The purpose of this paper is to describe the development of health educational program for maternity using principle of user centered design. Method: Research process includes five distinct phases: needs assessment, analysis, design, development/testing/revision, and application release. Results: This program includes an introduction, pregnancy test and fetal assessment, maternal and fetal change during pregnancy, self-care methods during pregnancy, complication of postpartum period, process of labor, complication of pregnancy, self care during the postpartum period, information for parenting skill, father page, FAQ, helping resources and institutions. Conclusion: The program will be distributed to health centers for maternal health education. The second phase of this project was evaluation this program for further development of the program. The end result of this program will be pregnant women with a high degree of usability. Author believe this program have true potential in helping maternal health promotion and successful parenting.

• Women's Health Nursing
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• v.9 no.3
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• pp.319-326
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• 2003

Objectives : A Pregnancy becomes a developmental challenge and a life-turning point for both the woman herself and her family. The purpose of this study was to identify the correlation amomg the family support, intention of pregnancy and the antenatal self-care of pregnant women. Method : The data were collected from 129 pregnant women by a questionnaire. The collected data were analyzed by descriptive statistics, t-test, ANOVA, Pearson's correlation coefficients, using the SPSS program. Result : The findings of the study are as fellows : Statistically significant variables related to family support were age, religion, educational level, occupation, relationship with husband, relationship with mother. Statistically significant variables related to intention of pregnancy were religion, abortion history. Statistically significant variables related to antenatal self-care were age, religion, occupation, abortion history, relationship with mother, the term of marriage. There was a significant correlation between family support and intention of pregnancy, family support and antenatal self-care, intention of pregnancy and antenatal self-care. Conclusion : Therefore, it is proposed that family support is an appropriate nursing intervention to improve the antenatal self-care and intention of pregnancy in pregnant women.

• The Journal of Korean Medicine
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• v.32 no.5
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• pp.25-40
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• 2011

Objectives: This study was performed to assess whether herbal medicine and acupuncture before in vitro fertilizationembryo transfer (IVF-ET) is effective on clinical pregnancy. Methods: From May 2010 to January 2011, a prospective analysis study was performed in 38 patients planning to undergo IVF-ET after taking herb medicine and acupuncture treatment. This study investigated the pregnancy rate and analyzed the change of dysmenorrhea by visual analog scale (VAS), body heat and condition of premenstrual syndrome (PMS), vaginal discharge and menstruation status. Results: 1. During herbal medicine and acupuncture treatment, five patients (13.16%) naturally became pregnant and six patients (15.79%) withdrew. After treatment, 15 patients (39.47%) received IVF-ET, 12 patients (31.58%) did not. 2. The biochemical pregnancy rate was 26.67%, the clinical pregnancy rate 26.67%, miscarriage rate 25% and ectopic pregnancy rate was 0%. 3. After treatment, PMS, dysmenorrhea and dysmenorrhea VAS was significantly decreased and the overall menstrual status improved. 4. After treatment, temperature difference of CV17-CV12 and CV4-CV12 increased, but it was not a statistically significant difference. 5. After treatment, decrease of hemoglobin and protein and increase of total bilirubin and creatinine were statistically significant. All the blood test results were within normal levels which proves safety of treatment. Conclusions: This study suggests that herbal medicine and acupuncture treatment before IVF-ET shows similar pregnancy rates with existing rates, but contributes to increasing the possibility of natural pregnancy.

• Korean Journal of Clinical Laboratory Science
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• v.45 no.4
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• pp.164-169
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• 2013

Red cell alloantibodies other than naturally occurring anti-A or anti-B are called unexpected red cell antibodies, and can be detected by performing an antibody screening. The frequency and distribution of unexpected antibody identified in Asan Medical Center were analyzed. We investigated a total of 135,238 cases of antibody screening test in AMC for 3 years from 2010 to 2012. Using column agglutination techniques, antibody identification tests were performed for the cases with positive antibody screening. Among 135,238 cases, 854 (0.6%) cases showed positive results of antibody screening test. In the order of frequency, 284 (33.3%) anti-Rh, 89 (10.4%) anti-MNS, 62 (7.3%) anti-Lewis, 34 (4.0%) anti-Kidd, 10 (1.2%) anti-Duffy, and 9 (1.1%) anti-P were identified. Multiple antibodies were detected in 199 (23.3%) cases. Among 381 subjects investigated for transfusion history, 299 (78.5%) had history of transfusion while 82 (21.5%) had unknown history. Thus the incidence of unexpected antibody was higher in the group with history of transfusion than the group without (p<0.001). Also, among 435 subjects investigated for the history of pregnancy, 46 (10.6%) had no history while 389 (89.4%) had history of pregnancy, showing higher incidence of unexpected antibody in the group with history of pregnancy than the group without pregnancy (p<0.001). Evaluated amounts and frequency of antigen exposure due to transfusion and pregnancy is suggested to increase the frequency of identification of unexpected antibody.

• Journal of Korean Biological Nursing Science
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• v.19 no.4
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• pp.258-265
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• 2017

Purpose: The purpose of this study was to examine the exercise patterns and the factors affecting exercise duration in pregnant women. Methods: The survey was conducted to assess walking and exercise duration in 146 pregnant participants, who live in Gyeongsang area. Data was analyzed with descriptive statistics, t-test, ANOVA and regression using the SPSS Win 23.0 program. Results: The average age of the participants was 31 years, and more than 87% of them were primigravida. The differences in the mean walking duration, the daily exercise duration, and the exercise days of participants before and during pregnancy were not statistically significant, but the exercise duration during pregnancy was decreased compared to that before pregnancy. The factors affecting weekly exercise duration were diagnosis of gestational diabetes mellitus, concern about weight gain during pregnancy, and monthly income. Conclusion: The results of this study show that development of an educational program to teach proper exercise practices during pregnancy is recommended.

• Journal of the Korea Convergence Society
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• v.12 no.8
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• pp.369-379
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• 2021

This study was a descriptive survey study to compare the stress, depression, fatigue, and sleep quality between pregnant women with gestational diabetes mellitus (GDM) and normal pregnant women. The study subjects were 49 gestational diabetes mellitus women and 51 normal pregnant women who were matching with gestational diabetes mellitus women by age, gestational age and number of births. The data collection was measured using structured questionnaires, numerical scales, and Canopy9 RSA(IEMBIO, Gangwondo, Korea) equipment. As a result of the study, pregnant women with gestational diabetes had higher pre-pregnancy weight(t=3.698, p<.001) and higher stress(t=4.505, p<.001), depression(t=4.564, p<.001), and fatigue(t=3.709, p<.001) during pregnancy, so weight management is important before pregnancy, and stress, fatigue, and depression relief nursing

• Women's Health Nursing
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• v.27 no.4
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• pp.388-404
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• 2021

Purpose: The purpose of this study was to examine the effects of team-based learning (TBL) on nursing students' communication ability, problem-solving ability, self-directed learning, and nursing knowledge related to high-risk pregnancy nursing. Methods: This quasi-experimental study used a nonequivalent control group pretest-posttest design. The participants were 91 nursing students allocated to an experimental group (n=45) and a control group (n=46). The experimental group received TBL lectures three times over the course of 3 weeks (100 minutes weekly) and the control group received instructor-centered lectures three times over the course of 3 weeks (100 minutes weekly). Data were collected by questionnaires from September to November, 2019. Data were analyzed using the chi-square test, paired t-test, and independent t-test. Results: After the intervention, the mean scores of problem-solving ability (t=-2.59, p=.011), self-directed learning (t=4.30, p<.001), and nursing knowledge (t=3.18, p=.002) were significantly higher in the experimental group than in the control group. No significant difference in communication ability was found between the experimental and control group (t=1.38, p=.171) Conclusion: The TBL program was effective for improving nursing students' problem-solving ability, self-directed learning, and nursing knowledge. Thus, TBL can be considered an effective teaching and learning method that can improve the learning outcomes of high-risk pregnancy nursing in women's health nursing classes. The findings suggest that TBL will be helpful for future nursing students to develop the nursing expertise necessary for providing nursing care to high-risk pregnant women.

• Journal of Microbiology and Biotechnology
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• v.32 no.6
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• pp.699-708
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• 2022

Antibiotic exposure during pregnancy have an adversely effects on offspring behavior and development. However, its mechanism is still poorly understood. To uncover this, we added ceftriaxone sodium to the drinking water of rats during pregnancy and conducted three-chamber sociability test, open-field test, and Morris water maze test in 3- and 6-week-old offspring. The antibiotic group offspring showed lower sociability and spatial learning and memory than control. To determine the role of the gut microbiota and their metabolites in the changes in offspring behavior, fecal samples of 6-week-old offspring rats were sequenced. The composition of dominant gut microbial taxa differed between the control and antibiotic groups. KEGG pathway analysis showed that S24-7 exerted its effects through the metabolic pathways including mineral absorption, protein digestion and absorption, Valine, leucine, and isoleucine biosynthesis. Correlation analysis showed that S24-7 abundance was negatively correlated with the level of VEGF, and metabolites associated with S24-7-including 3-aminobutanoic acid, dacarbazine, L-leucine, 3-ketosphinganine, 1-methylnicotinamide, and N-acetyl-L-glutamate-were also significantly correlated with VEGF levels. The findings suggest that antibiotic exposure during pregnancy, specifically ceftriaxone sodium, will adversely affects the behavior of offspring rats due to the imbalance of gut microbiota, especially S24-7, via VEGF and various metabolic pathways.

• Journal of Korean Physical Therapy Science
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• v.13 no.3
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• pp.41-54
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• 2006

The purpose of this study was to analyze various forms of musculoskeletal pain during different stages such as antenatal, pregnancy, delivery, and postpartum. The data for this study were collected through a self-administered survey with a structured questionnaire to 113 subjects. The survey was administered from May, 2003 to March, 2004 with subjects selected from postpartum centers in 3 different areas: Kangnam, Kangbook, and Bucheon. Analytical tools used for this study were frequency analysis, crosstabulation, percentage analysis, independent t-test, chi-square, and multiple response analysis. The findings of this study were as follows: 1. Comparison study of musculoskeletal pain according to variables including a pregnant woman's age, delivery techniques, different types of delivery such as primiparity, multiparity, dystocia, easy delivery, shows that these relationships are statistically not significant (p>0.05). 2. A total of 61.9% gave positive responses for the question about the relationship between musculoskeletal pain during postpartum and that in preparation of labor, but shows that this relationship was statistically not significant (p>0.05). 3. Relationships between forms of musculoskeletal pain in different phases are significant (p<0.05). The compared phases are: premarriage and pre-pregnancy, premarriage and antenatal, premarriage and postpartum, premarriage and lactiferous phase, pre-pregnancy and antenatal, pre-pregnancy and postpartum, and pre-pregnancy and lactiferous phase. However, results for the comparison between antenatal and postpartum, antenatal and lactiferous phase, and postpartum and lactiferous phase show that these relationships are statistically not significant (p>0.05). According to this study, musculoskeletal pain which occurred during antenatal is significantly related to the pain occurring during postpartum. Results produced from this study might be used as a helpful tool for developing educational programs aiming at teaching self pain-detection performable at home or at the workplace and body maintenance during the course of antenatal and postpartum.