Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Vascular Endothelial Growth Factor May Be Involved in the Behavioral Changes of Progeny Rats after Exposure to Ceftriaxone Sodium during Pregnancy

  • Yang, Xin (Department of Primary Child Health Care, Children's Hospital of Chongqing Medical University) ;
  • Tang, Ting (Department of Primary Child Health Care, Children's Hospital of Chongqing Medical University) ;
  • Li, Mengchun (Department of Pediatrics, Daping Hospital, Army Medical University) ;
  • Chen, Jie (Department of Pediatrics, Daping Hospital, Army Medical University) ;
  • Li, Tingyu (Department of Primary Child Health Care, Children's Hospital of Chongqing Medical University) ;
  • Dai, Ying (Department of Primary Child Health Care, Children's Hospital of Chongqing Medical University) ;
  • Cheng, Qian (Department of Primary Child Health Care, Children's Hospital of Chongqing Medical University)
  • Received : 2021.11.30
  • Accepted : 2022.04.19
  • Published : 2022.06.28
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Abstract

Antibiotic exposure during pregnancy have an adversely effects on offspring behavior and development. However, its mechanism is still poorly understood. To uncover this, we added ceftriaxone sodium to the drinking water of rats during pregnancy and conducted three-chamber sociability test, open-field test, and Morris water maze test in 3- and 6-week-old offspring. The antibiotic group offspring showed lower sociability and spatial learning and memory than control. To determine the role of the gut microbiota and their metabolites in the changes in offspring behavior, fecal samples of 6-week-old offspring rats were sequenced. The composition of dominant gut microbial taxa differed between the control and antibiotic groups. KEGG pathway analysis showed that S24-7 exerted its effects through the metabolic pathways including mineral absorption, protein digestion and absorption, Valine, leucine, and isoleucine biosynthesis. Correlation analysis showed that S24-7 abundance was negatively correlated with the level of VEGF, and metabolites associated with S24-7-including 3-aminobutanoic acid, dacarbazine, L-leucine, 3-ketosphinganine, 1-methylnicotinamide, and N-acetyl-L-glutamate-were also significantly correlated with VEGF levels. The findings suggest that antibiotic exposure during pregnancy, specifically ceftriaxone sodium, will adversely affects the behavior of offspring rats due to the imbalance of gut microbiota, especially S24-7, via VEGF and various metabolic pathways.

Keywords

Acknowledgement

We thank all the members who participated in the experiment for their help and suggestions in the whole work.

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