• The Journal of the Korea Contents Association
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• v.14 no.3
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• pp.52-58
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• 2014

Point of care testing (POCT) is could be easily and economically performed and was widely used. In particular, the test items using immune chromatographic method has been conveniently used. But when an error occurs, we should reaffirmed the test results, but we could not reaffirm because the test line is disappeared as the time pass, and the test results are different between laboratory workers. Therefore, we developed multi-strip reader which can store images for the test results and can interpretate the test results automatically. Then we evaluated newly developed multi-strip reader for urine pregnancy test, the test results of the multi-strip reader was 100% matched with the previously results. Our newly developed multi-strip reader was convenient and economic, and will be widely used in many laboratories.

• Journal of Microbiology and Biotechnology
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• v.20 no.6
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• pp.959-967
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• 2010

Down syndrome (DS) is an abnormality of the 21st chromosome that commonly occurs in children born to older women. Thus, amniotic fluid (AF) is usually collected from such women for prenatal diagnosis. This study analyzed human AF supernatants (AFS) using a mass spectrometric (MS) approach to search for candidate biomarkers of a DS pregnancy. The AFS were collected from older pregnant women at weeks 16-18 of their gestation by amniocentesis for cytogenetic analysis. The AFS from the pregnancies carrying DS (n=4) or chromosomally normal (n=6) fetuses, as revealed by the cytogenetic analysis, were then subjected to global protein profiling based on liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Affinity chromatography was also applied prior to the LC-ESI-MS/MS to minimize the masking effect of highly abundant albumin and immunoglobulin and thereby increase the diversity of the identified proteins. As a result, at least 30 new AFS proteins were identified and 44 AFS proteins were found to be differentially expressed between the DS and normal cases, where 6 of the proteins were unique to the DS cases and 11 were unique to the chromosomally normal cases. In addition, in the DS cases, 19 AFS proteins were downregulated and 8 were upregulated to varying degrees. A Western blot analysis confirmed the LC-ESI-MS/MS data, indicating that the combined detection of apolipoprotein A-II (apoA-II) and alpha-fetoprotein (AFP) could be a potential tool for diagnosing DS cases.

• Journal of Embryo Transfer
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• v.26 no.2
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• pp.91-96
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• 2011

The purpose of this study was to evaluate the effects of days open on subsequent reproductive performance following to estrus synchronization in the 114 lactating dairy cows. The animals were divided into two groups according to the time of estrus synchronization; viz, ${\leq}$ 85 days, and > 85 days postpartum, respectively. The estrus synchronization protocol consisted of insertion of a controlled internal drug release (CIDR) device containing 1.9 g progesterone with an injection of 250 ${\mu}g$ gonadorelin (Day 0), an injection of $PGF_2{\alpha}$ and removal of the device on Day 7, an injection of 250 ${\mu}g$ GnRH on Day 9, and TAI 17 h later. Pregnancy diagnosis was determined at 30 to 60 days after TAI using both ultrasonography and rectal palpation. The body condition score (BCS) gradually increased over the postpartum period. In estrus synchronized cows until 85 days, conception rate on first service, number of service per conception, interval from estrus synchronization to conception, and interval from calving to conception were not significantly different among two farms (P>0.05). In estrus synchronized cows after 85 days postpartum, conception rate on first service, number of service per conception and interval from calving to conception were significantly different ($P{\leq}0.05$) between herds A and B (26.8 vs 50.0%; $2.1{\pm}1.35$ vs $1.37{\pm}0.54$ times, $237.3{\pm}97.8$ vs $164.7{\pm}69.3$ days, respectively). In estrus synchronized cows after 85 days postpartum interval from estrus synchronization to conception was greater (P<0.01) in herd B than in herd A ($63.6{\pm}57.4$ vs $26.1{\pm}24.9$). These results indicate that the time of estrus synchronization for maximized the reproductive performance is before 85 days postpartum and feeding and management is important factor for high reproductive performance.

• Journal of Embryo Transfer
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• v.29 no.2
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• pp.157-161
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• 2014

The study aimed to determine the physical characteristics of estrus mucus and conception rates in dairy cattle. Samples of estrus mucus from the cervix were collected from 108 dairy cattle during heat and were examined for color and consistency. Samples were taken from bred animals at starting from day of breeding to the completion of one estrus cycle. The color of the cervical mucus was studied based on its transparency while the consistency was based on the thinness and thickness of the cervical mucus. The dairy cattle were bred and the pregnancy diagnosis was performed at the $60^{th}$ day post breeding. Findings showed that the estrus mucus of the dairy cattle was transparent in 58.3%, turbid in 31.5% and dirty in 10.2%. It was further observed that the mucus consistency of the dairy cattle was thin in 74.1% and thick in 25.9%. In the pregnant group, 67.3% mucus samples were found transparent, turbid in 23.6% and dirty in 9.1%. However, the corresponding figures for the non-pregnant group had 49.1%, 39.6% and 11.3%. The consistency of cervical mucus was found to be thin in 74.1% and thick in 25.9% of dairy cattle. The conception rates of dairy cattle with thin and thick consistency of cervical mucus were 81.8% and 18.2%, respectively. Pregnant was associated with consistency of cervical mucus (p<0.10). Findings indicated that dairy cattle with thin consistency of cervical mucus and had clear discharge were pregnant cows.

• Journal of Korean Library and Information Science Society
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• v.26
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• pp.131-168
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• 1997

The future of a country is influenced by the youth. Sound fostering of the youth is important for the country's future. Because of mechanization, industrialization and urbanization of our society, many social problems, such as confusion of the sense of value, dehumanization, money worshi n.0, ppings, juvenile delinquency are increasing. For the solution of problems of juvenile crime and dehumanization, bibliotherapy should be practiced in juvenile reformatories, schools and societies. The purpose of this study is to investigate the effectiveness and value of reading. And also the characteristics, brief history, principles and procedures of bibliotherapy are examined. In addition, the book catalog suitable for bibliotherapy is considered. The results of the study are as follows. (1) In order to solve the problem of dehumanization, and the confusion of the sense of value among young adults, we should enrich and cultivate the characters of young adults through the bibliotherapy. So, bibliotherapy for the treatment of human nature of juveniles should be practiced in the juvenile reformatories, schools and societies. (2) The values of reading in the past were inspiration, information and recreation. But, in recent times, the interest in therapeutic value of reading is increasing. (3) Bibliotherapy is a kind of psychotherapy using selected reading materials. A n.0, pplication scopes of bibliotherapy are very wides, including mental medicine, alcoholic poisoning, drug addiction, juvenile delinquency, counselling and education for school life, etc. (4) The principles of bibliotherapy consist of identification, catharsis, insight and it has self therapeutic principles through the reading. The procedures of bibliotherapy is similar to any other kind of psychotherapy: the participant's statement, diagnosis, treatment, continuous recognition of remedial value. (5) The well suited reading materials for bibliotherapy are literature and biography. Literature and biography are familiar to everyone, because they describe the mentalities, emotions and life styles of human beings. (6) The standard reading lists for bibliotherapy for the proper guidance of juvenile delinquency should be prepared for the solution of developmental tasks, such as, drinking, smoking, friendship, family, love, money, marriage, pregnancy, violence, etc. Finally, Bibliotherapy should be introduced to our school settings and corrections institutions for the proper guidance and prevention of the malpractice of the youths. In addition, we should be endeavor to develop the standard reading lists for bibliotherapy and case study.

• Journal of Sasang Constitutional Medicine
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• v.23 no.3
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• pp.361-373
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• 2011

1. Objectives: The purpose of this study was done to learn the Sasang constitutional distribution and to find out if there are differences in the type of diseases and symptoms according to the Sasang constitution in Japan. 2. Methods: We collected data from 366 patients who visited the Department of Oriental Medicine, Keio University and recruited 132 healthy persons in Tokyo, Japan. For sasang constitution diagnosis, they all have done SSCQ-P(Sasang Constitution Questionaire for Patients) questionnaire. and a sasang constitution specialist diagnosed the sasang constitution of them. And We classify the diseases and symptoms of 313 patients according to KCD(Korean Standard Classification of Diseases) and learn the prevalences of diseases and symptoms according to Sasang Constitution. 3. Results: 1) Among the total 498 subjects, distributional rate of Taeyangin, Soyangin, Taeeumin, and Soeumin were 2.0%, 26.3%, 29.9%, and 41.8%. Among the 366 patients, distributional rate of Taeyangin, Soyangin, Taeumin, and Soeumin were 0.8%, 27.3%, 28.7%, and 43.2%. Among the 132 healthy group, distributional rate of Taeyangin, Soyangin, Taeeumin, and Soeumin were 5.3%, 23.5%, 33.3%, and 37.9%. 2) The prevalences of 'V.Mental and behavioural disorders', 'XI.Diseases of the digestive system', 'XV.Pregnancy, childbirth and the puerperium' and 'feeling of coldness(X VIII.Symptoms, signs and abnormal clinical and laboratory findings, NEC)' of Soeumin were significantly higher than those of the other constitutions.(p-value<0.05) 4. Conclusions: The distributional rate of Sasangin of Japanese was different from that of Korean and especially the distributional rate of Soeumin of Japanese was significantly higher than that of Korean. There were significant differences on the prevalences of some diseases and symtoms according to KCD in Soeumin.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.23 no.4
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• pp.181-187
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• 2012

Objectives:The objective of this study was to examine the perinatal and developmental risk factors of attention-deficit hyperactivity disorder (ADHD) diagnosed with a structured interview among Korean children. Methods:The current study included 924 children (6-15 years) recruited from schools in five Korean cities or a child psychiatry outpatient clinic of Seoul National University Children's Hospital. The parents of the children completed the structured diagnostic interview for attention-deficit hyperactivity disorder, as well as questionnaires on perinatal and developmental risk factors. Results:Preterm delivery, severe maternal stress during pregnancy, change in primary care taker during the first three years, postpartum depression, and delayed first sentence showed a significant association with ADHD diagnosis. Conclusion:These findings suggest that perinatal and developmental factors contribute to development of ADHD in Korean children. Conduct of future research using a prospective design is needed in order to identify the causal relationship between observed risk factors and development of ADHD.

• Women's Health Nursing
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• v.22 no.2
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• pp.71-77
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• 2016

Purpose: This study was a comparative study to understand the levels of anxiety, pain and maternal-fetal attachment between women who became pregnant after infertility treatment and became pregnant naturally. Methods: This study used a comparative survey design. Data were collected by 50 couples of natural pregnancy and of who became pregnant after infertility treatment who visited delivery room in C Medical hospital, Seoul. These couples were to have first baby, and cervix dilatation of women was less than 3 cm regardless of diagnosis. Results: The score of anxiety of infertile women was significantly higher than that of naturally pregnant women; however, that of spouses showed no difference. The pain score for infertile women was significantly higher in both the active and transition phases. Pain scores that reported by their spouses did not show differences in either phase. The score of maternal-fetal attachment showed no difference between two groups of women. Conclusion: The result showed the importance of nursing intervention to reduce women's anxiety and pain, through both antenatal-childbirth education programs and assertive nursing interventions. It is necessary to develop and evaluate new intervention which would be more effective for reducing pain and anxiety for couples who became pregnant after infertility treatment.

• Clinical and Experimental Pediatrics
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• v.59 no.sup1
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• pp.64-67
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• 2016

Congenital tuberculosis (TB) is a rare disease that is associated with high mortality. Mycobacterium tuberculosis, the causative agent, may be transmitted from the infected mother to the fetus by the transplacental route or by aspiration of infected amniotic fluid. Clinical symptoms and signs are not specific. Miliary patterns are the most common findings in the chest X-rays of many infants with congenital TB. In this case, an 18-day-old boy had jaundice on the fifth day of birth, and fever and respiratory distress appeared on the 18th day. Chest X-ray showed diffuse fine bilateral infiltration. Clinically, pneumonia or sepsis was suspected. Respiratory symptoms and chest X-ray findings worsened despite empirical antibiotic therapy. The lungs showed miliary infiltration suggestive of TB. Gastric aspirates were positive for M. tuberculosis. Respiratory distress and fever were gradually improved after anti-TB medication. Congenital TB is difficult to detect because of minimal or no symptoms during pregnancy and nonspecific symptoms in neonates. Hence, clinicians should suspect the possibility of TB infection even if neonates have non-specific symptoms. Early diagnosis and meticulous treatment are required for the survival of neonates with TB.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.10
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• pp.1406-1415
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• 2013

The study was conducted to correlate the periparturient immune status in terms of neutrophil functions and cytokine expression in peripheral blood mononuclear cell culture with impending postpartum reproductive disorders in buffaloes. Forty pregnant buffaloes were observed for occurrence of postpartum reproductive disorders (PRD), i.e., metritis, endometritis and delayed uterine involution etc., during one week prepartum to four weeks postpartum period. A representative number (n = 6) of buffaloes that did not develop any PRD were included in group I (healthy, control), while the animals which experienced PRD were assigned into group II (PRD, n = 8). The blood samples were collected at weekly interval from one week prepartum to four weeks postpartum period considering the day of calving as 'd 0'. Differential leucocytes counts, superoxide and hydrogen peroxide production activity in isolated neutrophils and the mRNA expression profile of cytokines i.e., IL-2, IL-4 and IFN-${\gamma}$ in PBMC culture were studied in all the samples. A higher total leucocytes, neutrophil and band cells count along with impaired neutrophil functions i.e., lowered level of production of superoxide and hydrogen peroxide before parturition and during early postpartum period were observed in buffaloes developing PRD. Further, a lower expression of IL-2, IFN-${\gamma}$ and IL-4 mRNA in PBMC culture was observed at calving in buffaloes that subsequently developed PRD at later postpartum. Thus, suppression in neutrophil function and cytokine expression at prepartum to early postpartum period predisposes the buffaloes to develop postpartum reproductive disorders. Hence, monitoring of neutrophils function and cytokine expression profile would be effective to predict certain reproductive disorders at late pregnancy or immediately after parturition in buffaloes. In future, this may be a novel approach for determining suitable management and therapeutic decisions for prevention of commonly occurring reproductive disorders in farm animals.