Browse > Article
http://dx.doi.org/10.4014/jmb.0912.12035

Comparative Proteomic Analysis of Human Amniotic Fluid Supernatants with Down Syndrome Using Mass Spectrometry  

Park, Ji-Sook (Department of Molecular Biotechnology and Institute of Biomedical Science and Technology, Konkuk University)
Cha, Dong-Hyun (Department of Obstetrics and Gynecology, Kangnam CHA Hospital, Pochon CHA University, College of Medicine)
Jung, Jin-Woo (Department of Molecular Biotechnology and Institute of Biomedical Science and Technology, Konkuk University)
Kim, Young-Hwan (Mass Spectrometry Research Center, Korea Basic Science Institute)
Lee, Sook-Hwan (Department of Obstetrics and Gynecology, Kangnam CHA Hospital, Pochon CHA University, College of Medicine)
Kim, Young-Jun (Department of Applied Biochemistry, Konkuk University)
Kim, Kwang-Pyo (Department of Molecular Biotechnology and Institute of Biomedical Science and Technology, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.6, 2010 , pp. 959-967 More about this Journal
Abstract
Down syndrome (DS) is an abnormality of the 21st chromosome that commonly occurs in children born to older women. Thus, amniotic fluid (AF) is usually collected from such women for prenatal diagnosis. This study analyzed human AF supernatants (AFS) using a mass spectrometric (MS) approach to search for candidate biomarkers of a DS pregnancy. The AFS were collected from older pregnant women at weeks 16-18 of their gestation by amniocentesis for cytogenetic analysis. The AFS from the pregnancies carrying DS (n=4) or chromosomally normal (n=6) fetuses, as revealed by the cytogenetic analysis, were then subjected to global protein profiling based on liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Affinity chromatography was also applied prior to the LC-ESI-MS/MS to minimize the masking effect of highly abundant albumin and immunoglobulin and thereby increase the diversity of the identified proteins. As a result, at least 30 new AFS proteins were identified and 44 AFS proteins were found to be differentially expressed between the DS and normal cases, where 6 of the proteins were unique to the DS cases and 11 were unique to the chromosomally normal cases. In addition, in the DS cases, 19 AFS proteins were downregulated and 8 were upregulated to varying degrees. A Western blot analysis confirmed the LC-ESI-MS/MS data, indicating that the combined detection of apolipoprotein A-II (apoA-II) and alpha-fetoprotein (AFP) could be a potential tool for diagnosing DS cases.
Keywords
Proteomics; biomarker; Down syndrome; amniotic fluid; liquid chromatography-electrospray ionization-mass spectrometry;
Citations & Related Records

Times Cited By Web Of Science : 5  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Uhm, S. J., M. K. Gupta, J. H. Yang, S. H. Lee, and H. T. Lee. 2007. Selenium improves the developmental ability and reduces the apoptosis in porcine parthenotes. Mol. Reprod. Dev. 74: 1386-1394.   DOI   ScienceOn
2 Vascotto, C., A. M. Salzano, C. D'Ambrosio, A. Fruscalzo, D. Marchesoni, C. di Loreto, A. Scaloni, G. Tell, and F. Quadrifoglio. 2007. Oxidized transthyretin in amniotic fluid as an early marker of preeclampsia. J. Proteome Res. 6: 160-170.   DOI   ScienceOn
3 Nilsson, S., M. Ramstrom, M. Palmblad, O. Axelsson, and J. Bergquist. 2004. Explorative study of the protein composition of amniotic fluid by liquid chromatography electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. J. Proteome Res. 3: 884-889.   DOI   ScienceOn
4 Park, S. J., W. G. Yoon, J. S. Song, H. S. Jung, C. J. Kim, S. Y. Oh, et al. 2006. Proteome analysis of human amnion and amniotic fluid by two-dimensional electrophoresis and matrixassisted laser desorption/ionization time-of-flight mass spectrometry. Proteomics 6: 349-363.   DOI   ScienceOn
5 Roizen, N. J. and D. Patterson. 2003. Down's syndrome. Lancet 361: 1281-1289.   DOI   ScienceOn
6 Ahn, Y. H., E. S. Ji, J. Y. Lee, K. Cho, and J. S. Yoo. 2007. Coupling of TiO(2)-mediated enrichment and on-bead guanidinoethanethiol labeling for effective phosphopeptide analysis by matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun Mass Spectrom 21: 3987-3994.   DOI   ScienceOn
7 Hassan, M. I., V. Kumar, T. P. Singh, and S. Yadav. 2008. Proteomic analysis of human amniotic fluid from Rh(-) pregnancy. Prenat. Diagn. 28: 102-108.   DOI   ScienceOn
8 Zolotarjova, N., J. Martosella, G. Nicol, J. Bailey, B. E. Boyes, and W. C. Barrett. 2005. Differences among techniques for high-abundant protein depletion. Proteomics 5: 3304-3313.   DOI   ScienceOn
9 Michel, P. E., D. Crettaz, P. Morier, M. Heller, D. Gallot, J. D. Tissot, F. Reymond, and J. S. Rossier. 2006. Proteome analysis of human plasma and amniotic fluid by Off-Gel isoelectric focusing followed by nano-LC-MS/MS. Electrophoresis 27: 1169-1181.   DOI   ScienceOn
10 Queloz, P. A., D. Crettaz, L. Thadikkaran, V. Sapin, D. Gallot, J. Jani, et al. 2007. Proteomic analyses of amniotic fluid: Potential applications in health and diseases. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 850: 336-342.   DOI   ScienceOn
11 Rutanen, E. M., H. Bohn, and M. Seppala. 1982. Radioimmunoassay of placental protein 12: Levels in amniotic fluid, cord blood, and serum of healthy adults, pregnant women, and patients with trophoblastic disease. Am. J. Obstet. Gynecol. 144: 460-463.   DOI
12 Nadesalingam, J., A. L. Bernal, A. W. Dodds, A. C. Willis, D. J. Mahoney, A. J. Day, K. B. Reid, and N. Palaniyar. 2003. Identification and characterization of a novel interaction between pulmonary surfactant protein D and decorin. J. Biol. Chem. 278: 25678-25687.   DOI   ScienceOn
13 Michaels, J. E., S. Dasari, L. Pereira, A. P. Reddy, J. A. Lapidus, X. Lu, et al. 2007. Comprehensive proteomic analysis of the human amniotic fluid proteome: Gestational age-dependent changes. J. Proteome Res. 6: 1277-1285.   DOI   ScienceOn
14 Overgaard, M. T., E. S. Sorensen, D. Stachowiak, H. B. Boldt, L. Kristensen, L. Sottrup-Jensen, and C. Oxvig. 2003. Complex of pregnancy-associated plasma protein-A and the proform of eosinophil major basic protein. Disulfide structure and carbohydrate attachment. J. Biol. Chem. 278: 2106-2117.   DOI   ScienceOn
15 Pradhan, M., A. Dalal, F. Khan, and S. Agrawal. 2006. Fertility in men with Down syndrome: A case report. Fertil. Steril. 86: 1761-c1763.
16 Pulkkinen, L., T. Alitalo, T. Krusius, and L. Peltonen. 1992. Expression of decorin in human tissues and cell lines and defined chromosomal assignment of the gene locus (DCN). Cytogenet. Cell Genet. 60: 107-111.   DOI
17 Lubec, G., K. Krapfenbauer, and M. Fountoulakis. 2003. Proteomics in brain research: Potentials and limitations. Prog. Neurobiol. 69: 193-211.   DOI   ScienceOn
18 Miell, J. P., K. S. Langford, J. S. Jones, P. Noble, M. Westwood, A. White, and K. H. Nicolaides. 1997. The maternal insulin-like growth factor (IGF) and IGF-binding protein response to trisomic pregnancy during the first trimester: A possible diagnostic tool for trisomy 18 pregnancies. J. Clin. Endocrinol. Metab. 82: 287-292.   DOI   ScienceOn
19 Lee, S. J., K. H. Kim, J. S. Park, J. W. Jung, Y. H. Kim, S. K. Kim, et al. 2007. Comparative analysis of cell surface proteins in chronic and acute leukemia cell lines. Biochem. Biophys. Res. Commun. 357: 620-626.   DOI   ScienceOn
20 Liberatori, S., L. Bini, C. De Felice, B. Magi, B. Marzocchi, R. Raggiaschi, et al. 1997. A two-dimensional protein map of human amniotic fluid at 17 weeks' gestation. Electrophoresis 18: 2816-2822.   DOI   ScienceOn
21 Mauk, M. R., F. I. Rosell, B. Lelj-Garolla, G. R. Moore, and A. G. Mauk. 2005. Metal ion binding to human hemopexin. Biochemistry 44: 1864-1871.   DOI   ScienceOn
22 Mazurkiewicz, J. C., G. F. Watts, F. G. Warburton, B. M. Slavin, C. Lowy, and E. Koukkou. 1994. Serum lipids, lipoproteins and apolipoproteins in pregnant non-diabetic patients. J. Clin. Pathol. 47: 728-731.   DOI   ScienceOn
23 Hsiang, Y. H., G. D. Berkovitz, G. L. Bland, C. J. Migeon, and A. C. Warren. 1987. Gonadal function in patients with Down syndrome. Am. J. Med. Genet. 27: 449-458.   DOI   ScienceOn
24 Friberg, H. and T. Wieloch. 2002. Mitochondrial permeability transition in acute neurodegeneration. Biochimie 84: 241-250.   DOI   ScienceOn
25 Huether, C. A., J. Ivanovich, B. S. Goodwin, E. L. Krivchenia, V. S. Hertzberg, L. D. Edmonds, D. S. May, and J. H. Priest. 1998. Maternal age specific risk rate estimates for Down syndrome among live births in whites and other races from Ohio and metropolitan Atlanta, 1970-1989. J. Med. Genet. 35: 482-490.   DOI
26 Kubota, T. and H. Takeuchi. 1998. Evaluation of insulin-like growth factor binding protein-1 as a diagnostic tool for rupture of the membranes. J. Obstet. Gynaecol. Res. 24: 411-417.   DOI   ScienceOn
27 Langford, K., W. Blum, K. Nicolaides, J. Jones, A. McGregor, and J. Miell. 1994. The pathophysiology of the insulin-like growth factor axis in fetal growth failure: A basis for programming by undernutrition? Eur. J. Clin. Invest. 24: 851-856.   DOI   ScienceOn
28 Fountoulakis, M. 2001. Proteomics: Current technologies and applications in neurological disorders and toxicology. Amino Acids 21: 363-381.   DOI   ScienceOn
29 Freidl, M., T. Gulesserian, G. Lubec, M. Fountoulakis, and B. Lubec. 2001. Deterioration of the transcriptional, splicing and elongation machinery in brain of fetal Down syndrome. J. Neural Transm. Suppl. 61: 47-57.
30 Gupta, M. K., J. M. Jang, J. W. Jung, S. J. Uhm, K. P. Kim, and H. T. Lee. 2009. Proteomic analysis of parthenogenetic and in vitro fertilized porcine embryos. Proteomics 9: 2846-2860.   DOI   ScienceOn
31 Hassell, J. R., J. H. Kimura, and V. C. Hascall. 1986. Proteoglycan core protein families. Annu. Rev. Biochem. 55: 539-567.   DOI   ScienceOn
32 Hook, E. B. and A. Lindsjo. 1978. Down syndrome in live births by single year maternal age interval in a Swedish study: Comparison with results from a New York State study. Am. J. Hum. Genet. 30: 19-27.
33 Christiansen, M., I. Jaliashvili, M. T. Overgaard, C. Ensinger, P. Obrist, and C. Oxvig. 2000. Quantification and characterization of pregnancy-associated complexes of angiotensinogen and the proform of eosinophil major basic protein in serum and amniotic fluid. Clin. Chem. 46: 1099-1105.
34 Bobrow, M., T. Barby, A. Hajianpour, D. Maxwell, and S. C. Yau. 1992. Fertility in a male with trisomy 21. J. Med. Genet. 29: 141.
35 Buhimschi, C. S., V. Bhandari, B. D. Hamar, M. O. Bahtiyar, G. Zhao, A. K. Sfakianaki, et al. 2007. Proteomic profiling of the amniotic fluid to detect inflammation, infection, and neonatal sepsis. PLoS Med. 4: e18.   DOI
36 Buhimschi, I. A., E. Zambrano, C. M. Pettker, M. O. Bahtiyar, M. Paidas, V. A. Rosenberg, S. Thung, C. M. Salafia, and C. S. Buhimschi. 2008. Using proteomic analysis of the human amniotic fluid to identify histologic chorioamnionitis. Obstet. Gynecol. 111: 403-412.   DOI   ScienceOn
37 Zuhlke, C., U. Thies, I. Braulke, A. Reis, and C. Schirren. 1994. Down syndrome and male fertility: PCR-derived fingerprinting, serological and andrological investigations. Clin. Genet. 46: 324-326.
38 Aebersold, R. and M. Mann. 2003. Mass spectrometry-based proteomics. Nature 422: 198-207.   DOI   ScienceOn
39 Antonarakis, S. E., R. Lyle, E. T. Dermitzakis, A. Reymond, and S. Deutsch. 2004. Chromosome 21 and Down syndrome: From genomics to pathophysiology. Nat. Rev. Genet. 5: 725-738.   DOI   ScienceOn
40 Benn, P. A., J. Ying, T. Beazoglou, and J. F. Egan. 2001. Estimates for the sensitivity and false-positive rates for second trimester serum screening for Down syndrome and trisomy 18 with adjustment for cross-identification and double-positive results. Prenat. Diagn. 21: 46-51.   DOI   ScienceOn
41 Wald, N. J., H. S. Cuckle, J. W. Densem, K. Nanchahal, P. Royston, T. Chard, et al. 1988. Maternal serum screening for Down's syndrome in early pregnancy. Br. Med. J. 297: 883-887.   DOI   ScienceOn
42 Wang, H. S., L. A. Perry, J. Kanisius, R. K. Iles, J. M. Holly, and T. Chard. 1991. Purification and assay of insulin-like growth factor-binding protein-1: Measurement of circulating levels throughout pregnancy. J. Endocrinol. 128: 161-168.   DOI   ScienceOn
43 Thadikkaran, L., D. Crettaz, M. A. Siegenthaler, D. Gallot, V. Sapin, R. V. Iozzo, P. A. Queloz, P. Schneider, and J. D. Tissot. 2005. The role of proteomics in the assessment of premature rupture of fetal membranes. Clin. Chim. Acta 360: 27-36.   DOI   ScienceOn
44 Xaus, J., M. Comalada, M. Cardo, A. F. Valledor, and A. Celada. 2001. Decorin inhibits macrophage colony-stimulating factor proliferation of macrophages and enhances cell survival through induction of p27(Kip1) and p21(Waf1). Blood 98: 2124-2133.   DOI   ScienceOn
45 Tsangaris, G. T., P. Karamessinis, A. Kolialexi, S. D. Garbis, A. Antsaklis, A. Mavrou, and M. Fountoulakis. 2006. Proteomic analysis of amniotic fluid in pregnancies with Down syndrome. Proteomics 6: 4410-4419.   DOI   ScienceOn
46 Vuadens, F., C. Benay, D. Crettaz, D. Gallot, V. Sapin, P. Schneider, et al. 2003. Identification of biologic markers of the premature rupture of fetal membranes: Proteomic approach. Proteomics 3: 1521-1525.   DOI   ScienceOn
47 Schonherr, E., M. Broszat, E. Brandan, P. Bruckner, and H. Kresse. 1998. Decorin core protein fragment Leu155-Val260 interacts with TGF-beta but does not compete for decorin binding to type I collagen. Arch. Biochem. Biophys. 355: 241-248.   DOI   ScienceOn
48 Sheridan, R., J. Llerena Jr., S. Matkins, P. Debenham, A. Cawood, and M. Bobrow. 1989. Fertility in a male with trisomy 21. J. Med. Genet. 26: 294-298.   DOI
49 Smith, A. and W. T. Morgan. 1981. Hemopexin-mediated transport of heme into isolated rat hepatocytes. J. Biol. Chem. 256: 10902-10909.
50 Takahashi, N., Y. Takahashi, and F. W. Putnam. 1985. Complete amino acid sequence of human hemopexin, the heme-binding protein of serum. Proc. Natl. Acad. Sci. U.S.A. 82: 73-77.   DOI   ScienceOn
51 Tsangaris, G. T., A. Kolialexi, P. M. Karamessinis, A. K. Anagnostopoulos, A. Antsaklis, M. Fountoulakis, and A. Mavrou. 2006. The normal human amniotic fluid supernatant proteome. In Vivo 20: 479-490.
52 Qian, W. J., T. Liu, M. E. Monroe, E. F. Strittmatter, J. M. Jacobs, L. J. Kangas, K. Petritis, D. G. Camp 2nd, and R. D. Smith. 2005. Probability-based evaluation of peptide and protein identifications from tandem mass spectrometry and SEQUEST analysis: The human proteome. J. Proteome Res. 4: 53-62.   DOI   ScienceOn