• 한국자원식물학회지
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• 제33권6호
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• pp.689-695
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• 2020

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'Milky way'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 µl PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

• 한국전기전자재료학회논문지
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• 제31권7호
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• pp.450-454
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• 2018

Because of the rapidly changing environment and high uncertainties, the semiconductor industry is in need of appropriate forecasting technology. In particular, both the cost and time in the test process are increasing because the process becomes complicated and there are more factors to consider. In this paper, we propose a prediction model that predicts a final "good" or "bad" on the basis of preconditioning test data generated in the semiconductor test process. The proposed prediction model solves the classification and regression problems that are often dealt with in the semiconductor process and constructs a reliable prediction model. We also implemented a prediction model through various machine learning algorithms. We compared the performance of the prediction models constructed through each algorithm. Actual data of the semiconductor test process was used for accurate prediction model construction and effective test verification.

• Nuclear Engineering and Technology
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• 제49권6호
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• pp.1310-1317
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• 2017

GASFLOW-MPI is a widely used scalable computational fluid dynamics numerical tool to simulate the fluid turbulence behavior, combustion dynamics, and other related thermal-hydraulic phenomena in nuclear power plant containment. An efficient scalable linear solver for the large-scale pressure equation is one of the key issues to ensure the computational efficiency of GASFLOW-MPI. Several advanced Krylov subspace methods and scalable preconditioning methods are compared and analyzed to improve the computational performance. With the help of the powerful computational capability, the large eddy simulation turbulent model is used to resolve more detailed turbulent behaviors. A backward-facing step flow is performed to study the free shear layer, the recirculation region, and the boundary layer, which is widespread in many scientific and engineering applications. Numerical results are compared with the experimental data in the literature and the direct numerical simulation results by GASFLOW-MPI. Both time-averaged velocity profile and turbulent intensity are well consistent with the experimental data and direct numerical simulation result. Furthermore, the frequency spectrum is presented and a -5/3 energy decay is observed for a wide range of frequencies, satisfying the turbulent energy spectrum theory. Parallel scaling tests are also implemented on the KIT/IKET cluster and a linear scaling is realized for GASFLOW-MPI.

• 한국추진공학회지
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• 제4권4호
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• pp.59-69
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• 2000

2차인 압축성/비압축성 Navier-Stokes 방정식은 이용하여 DCA 압푹기 익렬의 수치 해석을 수행하고 실험치와 비교 검토하였다. SIMPLE 알고리즘을 적용한 비압축성 코드는 대류항의 이산화에 하이브리드 도식을 진동해를 방지하기 위해 집중격자 기법을 사용하였다. 압축성 코드는 예조건화 기법 을 적용하였으며 공간 이산화출 위해 풍상 차분법을, 시간 적분을 위해서는 LU-SGS 기법을 사용하였다. 또한 난류 점성 유동장을 해석하기 위해 Baldwin-Lomax, standard $\kappa$ -$\varepsilon$, $\kappa$ -$\varepsilon$ Lam. Bremhorst, standard $\kappa$-$\omega$, $\kappa$ -$\omega$ SST 모델 등의 난류 모델을 적용하여 각 모델들의 특성을 살펴보았다.

• Biomolecules & Therapeutics
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• 제27권4호
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• pp.404-413
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• 2019

Udenafil, which is a $PDE_5$ inhibitor, is used to treat erectile dysfunction. However, it is unclear whether udenafil induces hair growth via the stimulation of adipose-derived stem cells (ASCs). In this study, we investigated whether udenafil stimulates ASCs and whether increased growth factor secretion from ASCs to facilitate hair growth. We found that subcutaneous injection of udenafiltreated ASCs accelerated telogen-to-anagen transition in vivo. We also observed that udenafil induced proliferation, migration and tube formation of ASCs. It also increased the secretion of growth factors from ASCs, such as interleukin-4 (IL-4) and IL12B, and the phosphorylation of ERK1/2 and $NF{\kappa}B$. Furthermore, concomitant upregulation of IL-4 and IL12B mRNA levels was attenuated by ERK inhibitor or $NF{\kappa}B$ knockdown. Application of IL-4 or IL12B enhanced anagen induction in mice and increased hair follicle length in organ culture. The results indicated that udenafil stimulates ASC motility and increases paracrine growth factor, including cytokine signaling. Udenafil-stimulated secretion of cytokine from ASCs may promote hair growth via the ERK and $NF{\kappa}B$ pathways. Therefore, udenafil can be used as an ASC-preconditioning agent for hair growth.

• The Korean Journal of Physiology and Pharmacology
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• 제25권4호
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• pp.261-272
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• 2021

Doxorubicin (Dox) is widely used to the treatment of cancer, however, it could cause damage to gastric mucosa. To investigate the protective effects and related mechanisms of coenzyme Q10 (CoQ10) and vitamin C (VC) on Dox-induced gastric mucosal injury, we presented the survey of the 4 groups of the rats with different conditions. The results showed Dox treatment significantly induced GES-1 apoptosis, but preconditioning in GES-1 cells with VC or CoQ10 significantly inhibited the Dox-induced decrease and other harm effects, including the expression and of IκKβ, IκBα, NF-κB/p65 and tumor necrosis factor (TNF-α) in GES-1 cells. Moreover, high-throughput sequencing results showed Dox treatment increased the number of harmful gut microbes, and CoQ10 and VC treatment inhibited this effect. CoQ10 and VC treatment inhibits Dox-induced gastric mucosal injury by inhibiting the activation of the IkKB/IκBα/NF-κB/p65/TNF-α pathway, promoting anti-inflammatory effects of gastric tissue and regulating the composition of the intestinal flora.

• 한국동물생명공학회지
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• 제37권2호
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• pp.149-154
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• 2022

Busulfan is the most commonly used drug for preconditioning during the transplantation of hematopoietic stem cells and male germ cells. Here, we describe side effects of high doses of busulfan in male mongrel dogs. Busulfan was intravenously administered to three groups of dogs at doses of 10, 15, and 17.5 mg/kg body weight. The total white blood cell, neutrophil, eosinophil, lymphocyte, monocyte, and platelet counts steadily reduced in a dose-dependent manner following busulfan treatment. The white blood cell, neutrophil, and monocyte counts recovered after 6 weeks of busulfan treatment, however, the eosinophil, lymphocyte, and platelet counts remained unaltered. Additionally, there was one fatality in the each of the groups that were administered 15 and 17.5 mg/kg busulfan. The gross lesions included severe hemorrhage in the stomach, intestinal tracts, mesentery and urinary bladder. Microscopic investigation revealed severe pulmonary edema and hemorrhage in the lungs, and severe multifocal to coalescing transmural hemorrhage in the intestines and urinary bladder. These results indicated that treatment with busulfan at doses higher than 15 mg/kg initiates severe bleeding in the internal organs and can have fatal results.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.35-35
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• 2021

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'MilkyWay'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 ㎕ PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

• 한국통신학회논문지
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• 제34권12A호
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• pp.925-932
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• 2009

최근에 온도관리가 필수조건인 신선식품의 유통관리 분야에서 전지 지원 수동형 센서태그에 대한 요구가 크게 증대되고 있다. ISO/IEC18000-6REV1은 산업체에서 널리 사용되고 있는 EPCglobal Class1 Generation2 규격의 RFID 태그와 호환되는 전지 지원 수동형 센서태그를 지원하는 표준을 규정하고 있다. 본 논문에서는 전지 지원 수동형 센서태그를 지원하는 리더 모뎀을 FPGA로 설계하였으며, ISO/IEC18000-6REV1에서 정의하는 센서 데이터 처리기능을 개발하였다. 모뎀의 송신 블록은 표준에서 규정하는 성형필터(pulse shaping filter)를 지원하며, RF출력 신호는 표준에서 권고하는 스펙트럼 마스크를 만족한다. 태그의 신호를 수신하는 모뎀의 수신 블록은 심벌 타이밍 동기에 널리 사용되는 Gardner TED(Timing Error Detection) 방법을 이용하였으며, 동기 방식으로 설계된 수신기는 FM0, Miller-2, Miller-4, 그리고 Miller-8 신호를 모두 수신할 수 있다. 본 논문에서는 표준화가 진행중인 ISO/IEC18000-6REV1 규격을 만족하는 모뎀과 센서태그용 리더 시스템을 개발하여 센서태그 및 수동형 태그를 무선 환경에서 안정적으로 인식하였으며, 임베디드 리눅스 기반 플랫폼에서 센서 프로토콜을 구현하여 센서 데이터를 실시간으로 처리하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제9권4호
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• pp.187-193
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• 2005

Several signal transduction pathways have been implicated in ischemic preconditioning induced by the activation of ATP-sensitive $K^+$ $(K_{ATP})$ channels. We examined whether protein kinase C (PKC) modulated the activity of $K_{ATP}$ channels by recording $K_{ATP}$ channel currents in rabbit ventricular myocytes using patch-clamp technique and found that phorbol 12,13-didecanoate (PDD) enhanced pinacidil-induced $K_{ATP}$ channel activity in the cell-attached configuration; and this effect was prevented by bisindolylmaleimide (BIM). $K_{ATP}$ channel activity was not increased by $4{\alpha}-PDD$. In excised insideout patches, PKC stimulated $K_{ATP}$ channels in the presence of 1 mM ATP, and this effect was abolished in the presence of BIM. Heat-inactivated PKC had no effect on channel activity. PKC-induced activation of $K_{ATP}$ channels was reversed by PP2A, and this effect was not detected in the presence of okadaic acid. These results suggest that PKC activates $K_{ATP}$ channels in rabbit ventricular myocytes.