Browse > Article

Protein Kinase C Activates ATP-sensitive Potassium Channels in Rabbit Ventricular Myocytes  

Kim, Na-Ri (Mitochondrial Signaling Laboratory, Department of Physiology and Biophysics, College of Medicine, Cardiovascular & Metabolic Disease Center, Biohealth Products Research Center, Inje University)
Youm, Jae-Boum (Mitochondrial Signaling Laboratory, Department of Physiology and Biophysics, College of Medicine, Cardiovascular & Metabolic Disease Center, Biohealth Products Research Center, Inje University)
Joo, Hyun (Mitochondrial Signaling Laboratory, Department of Physiology and Biophysics, College of Medicine, Cardiovascular & Metabolic Disease Center, Biohealth Products Research Center, Inje University)
Kim, Hyung-Kyu (Mitochondrial Signaling Laboratory, Department of Physiology and Biophysics, College of Medicine, Cardiovascular & Metabolic Disease Center, Biohealth Products Research Center, Inje University)
Kim, Eui-Yong (Mitochondrial Signaling Laboratory, Department of Physiology and Biophysics, College of Medicine, Cardiovascular & Metabolic Disease Center, Biohealth Products Research Center, Inje University)
Han, Jin (Mitochondrial Signaling Laboratory, Department of Physiology and Biophysics, College of Medicine, Cardiovascular & Metabolic Disease Center, Biohealth Products Research Center, Inje University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.9, no.4, 2005 , pp. 187-193 More about this Journal
Abstract
Several signal transduction pathways have been implicated in ischemic preconditioning induced by the activation of ATP-sensitive $K^+$ $(K_{ATP})$ channels. We examined whether protein kinase C (PKC) modulated the activity of $K_{ATP}$ channels by recording $K_{ATP}$ channel currents in rabbit ventricular myocytes using patch-clamp technique and found that phorbol 12,13-didecanoate (PDD) enhanced pinacidil-induced $K_{ATP}$ channel activity in the cell-attached configuration; and this effect was prevented by bisindolylmaleimide (BIM). $K_{ATP}$ channel activity was not increased by $4{\alpha}-PDD$. In excised insideout patches, PKC stimulated $K_{ATP}$ channels in the presence of 1 mM ATP, and this effect was abolished in the presence of BIM. Heat-inactivated PKC had no effect on channel activity. PKC-induced activation of $K_{ATP}$ channels was reversed by PP2A, and this effect was not detected in the presence of okadaic acid. These results suggest that PKC activates $K_{ATP}$ channels in rabbit ventricular myocytes.
Keywords
$K_{ATP}$ channel; Patch-clamp electrophysiology; Protein kinase C; Phorbol 12,13-didecanoate; Bisindolylmaleimide;
Citations & Related Records

Times Cited By SCOPUS : 1
연도 인용수 순위
1 Colquhoun D, Sigworth FJ. Fitting and statistical analysis of single channel records. In: Sakmann B, Neher E ed, Single-Channel Recording. 1st ed. New York, Plenum, p 191-263, 1983
2 Grover GJ, Sleph PG, Dzwonczyk S. Role of myocardial ATPsensitive potassium channels in mediating preconditioning in the dog heart and their possible interaction with adenosine $A_{1}$-receptors. Circulation 86: 1310-1316, 1992   DOI   PUBMED   ScienceOn
3 Hu K, Duan D, Li GR, Nattel S. Protein kinase C activates ATPsensitive $K^+$ current in human and rabbit ventricular myocytes. Circ Res 78: 492-498, 1996   DOI   PUBMED   ScienceOn
4 Jenkins DP, Kerac M, Yellon DM. Preconditioning: protein kinase C and the KATP channel in isolated rat heart. J Mol Cell Cardiol 27: A142 Abstract, 1995
5 Liu Y, Ytrehus K, Downey JM. Evidence that translocation of protein kinase C is a key event during ischemic preconditioning of rabbit myocardium. J Mol Cell Cardiol 26: 661-668, 1994   DOI   ScienceOn
6 Otani H, Okada T, Fujiwara H, Uchiyama T, Sumida T, Kido M, Imamura H. Combined pharmacological preconditioning with a G-protein-coupled receptor agonist, a mitochondrial $K_{ATP}$ channel opener and a nitric oxide donor mimics ischemic preconditioning. Clin Exp Pharmacol Physiol 30: 684-693, 2003   DOI   ScienceOn
7 Van Winkle DM, Kuzume K, Dote K, Wolff RA. Infarct limitation by protein kinase C (PKC) is attenuated by blockade of ATPsensitive potassium ($K_{ATP}$) channels. J Mol Cell Cardiol 27: A142 Abstract, 1995
8 Venkatesh N, Lamp ST, Weiss JN. Sulfonylureas, ATP-sensitive K+ channels, and cellular $K^{+}$ loss during hypoxia, ischemia and metabolic inhibition in mammalian ventricle. Circ Res 69: 623-637, 1991   DOI   PUBMED   ScienceOn
9 Ribalet B, Eddlestone GT. Characterization of the G protein coupling of a somatostatin receptor in the $K_{ATP}$ channel in insulin-secreting mammalian cell lines. J Physiol (Lond) 485: 73-86, 1995
10 Uchiyama Y, Otani H, Wakeno M, Okada T, Uchiyama T, Sumida T, Kido M, Imamura H, Nakao S, Shingu K. Role of mitochondrial $K_{ATP}$ channels and protein kinase C in ischemic preconditioning. Clin Exp Pharmacol Physiol 30: 426-436, 2003   DOI   ScienceOn
11 Parratt JR, Kane KA. KATP channels and ischemic preconditioning. Cardiovasc Res 28: 783-787, 1994   DOI   ScienceOn
12 Faivre JF, Findlay I. Action potential duration and activation of the ATP-sensitive potassium current in isolated guinea pig ventricular myocytes. Biochem Biophys Acta 1029: 167-172, 1990   DOI   ScienceOn
13 Bonev AD, Nelson MT. Muscarinic inhibition of ATP-sensitive $K^{+}$ channels by protein kinase C in urinary bladder smooth muscle. Am J Physiol 265: C1723－C1728, 1993
14 Van Winkle DM, Chien GL, Wolff RA, Soifer BE, Kuzume K, Davis RF. Cardioprotection provided by adenosine receptor activation is abolished by blockade of the $K_{ATP}$ channel. Am J Physiol 266: H829-839, 1994   PUBMED
15 Moon CH, Kim MY, Kim MJ, Lee S, Yi KY, Yoo SE, Lee DH, Lim H, Kim HS, Lee SH, Baik EJ, Jung YS. KR-31378, a novel benzopyran analog, attenuates hypoxia-induced cell death via mitochondrial $K_{ATP}$ channel and protein kinase C-epsilon in heart-derived H9c2 cells. Eur J Pharmacol 506: 27-35, 2004   DOI   PUBMED   ScienceOn
16 Dunne MJ. Phorbol myristate acetate and ATP-sensitive potassium channels in insulin-secreting cells. Am J Physiol 267: C501-506, 1994   DOI   PUBMED
17 Light PE, Allen BG, Walsh MP, French RJ. Regulation of adenosine triphosphate-sensitive potassium channels from rabbit ventricular myocytes by protein kinase C and type 2A protein phosphatase.Biochemistry 34: 7252-7257, 1995   DOI   ScienceOn
18 Wang N, Minatoguchi S, Chen XH, Uno Y, Lu C, Misao Y, Nagai H, Takemura G, Fujiwara H. Benidipine reduces myocardial infarct size involving reduction of hydroxyl radicals and production of protein kinase C-dependent nitric oxide in rabbits. J Cardiovasc Pharmacol 43: 747-757, 2004   DOI   ScienceOn
19 Yao Z, Gross GJ. A comparison of adenosine-induced cardioprotection and ischemic preconditioning in dogs: efficacy, time course, and role of $K_{ATP}$ channels. Circulation 89: 1229-1236, 1994   DOI   PUBMED   ScienceOn
20 Liu GS, Thornton J, Van Winkle DM, Stanley AWH, Olsson RA, Downey JM. Protection against infarction afforded preconditioning is mediated by A1 adenosine receptors in rat heart. Circulation 84: 350-356, 1991   DOI   PUBMED   ScienceOn
21 Speechly-Dick ME, Grover GJ, Yellon DM. Does ischemic preconditioning in the human involve protein kinase C and ATP-dependent $K^+$ channel?: studies of contractile function after stimulated ischemia in an atrial in vitro model. Circ Res 77: 1030-1035, 1995   DOI   PUBMED   ScienceOn
22 Sanada S, Kitakaze M. Ischemic preconditioning: emerging evidence, controversy, and translation trials. Int J Cardiol 97: 263-276, 2004   DOI   ScienceOn
23 Lebuffe G, Schumacker PT, Shao ZH, Anderson T, Iwase H, Vanden Hoek TL. ROS and NO trigger early preconditioning: relationship to mitochondrial $K_{ATP}$ channel. Am J Physiol Heart Circ Physiol 284: H299-H308, 2004
24 Davis NW, Standen NB, Stanfield PR. ATP-dependent potassium channels of muscle cells: their properties, regulation, and possible functions. J Bioenerg Biomembr 23: 509-535, 1991   DOI   PUBMED
25 Han J, Kim E, So I, Earm YE. ATP-sensitive potassium channels are modulated by intracellular lactate in rabbit ventricular myocytes. Pflugers Arch 425: 546-548, 1993   DOI   PUBMED
26 Wang W, Giebisch G. Dual modulation of renal ATP-sensitive $K^{+}$ channel by protein kinase A and C. Proc Natl Acad Sci USA 88: 9722-9725, 1991   DOI   ScienceOn
27 Honore E, Lazdunski M. Hormone-regulated $K^+$ channels in follicle enclosed oocytes are activated by vasorelaxung $K^+$ channel openers and blocked by antidiabetic sulphonylureas. Proc Natl Acad Sci USA 88: 5438-5442, 1991a6b   DOI   ScienceOn
28 Walsh RS, Tsuchida A, Daly JJF, Thornton JD, Cohen MV, Downey JM. Ketamine-xylazine anaesthesia permits a $K_{ATP}$ channel antagonist to attenuate preconditioning in rabbit myocardium. Cardiovasc Res 28: 1337-1341, 1994   DOI   ScienceOn
29 Hamill OP, Marty A, Nether E, Sakmann B, Sigworth FJ. Improved patch-clamp techniques for high-resolution current recording from cell-free membrane patches. Pflugers Arch 391: 85-100, 1981   DOI   ScienceOn
30 Parratt JR. Protection of the heart by ischemic preconditioning: mechanisms and possibilities for pharmacological exploitation. Trends Pharmacol Sci 15: 19-25, 1994,   DOI   PUBMED   ScienceOn
31 Ytehus K, Liu Y, Downey JM. Preconditioning protects ischemic rabbit heart by protein kinase C activation. Am J Physiol 266: H1145-H1152, 1994   PUBMED
32 Okawa H, Horimoto H, Mieno S, Nomura Y, Yoshida M, Shinjiro S. Preischemic infusion of alpha-human atrial natriuretic peptide elicits myoprotective effects against ischemia reperfusion in isolated rat hearts. Mol Cell Biochem 248: 171-177, 2003   DOI   PUBMED   ScienceOn