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http://dx.doi.org/10.7732/kjpr.2020.33.6.689

Preconditioning for Cryopreservation of in vitro Grown Bulblets of Lily using Droplet-Vitrification  

Song, Jae-young (National Agrobiodiversity Center, NAS, RDA)
Lee, Young-yi (National Agrobiodiversity Center, NAS, RDA)
Yi, Jung-yoon (National Agrobiodiversity Center, NAS, RDA)
Lee, Jung-ro (National Agrobiodiversity Center, NAS, RDA)
Yoon, Mun-sup (National Agrobiodiversity Center, NAS, RDA)
Publication Information
Korean Journal of Plant Resources / v.33, no.6, 2020 , pp. 689-695 More about this Journal
Abstract
This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'Milky way'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 µl PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.
Keywords
Bulb; Cryopreservation; Droplet-vitrification; Lilium; Regrowth;
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