Kim, Jin-Seog;Lee, Byung-Hoi;Kim, So-Hee;Min, Suk-Ki;Choi, Jung-Sup
Journal of Plant Biotechnology
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v.33
no.1
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pp.57-62
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2006
Several methods for determining the response of corn to glyphosate were investigated to provide a fast and reliable method for identifying glyphosate-resistant corn in vivo. Two bioassays were developed. One assay is named 'whole plant / leaf growth assay', in which the herbicide glyphosate is applied on the upper part of 3rd leaf and the growth of herbicide-untreated 4th leaf is measured at 3 day after treatment. in this assay, the leaf growth of conventional corn was inhibited in a dose dependent from 50 to $1600{\mu}g/mL$ of glyphosate and growth inhibition at $1600{\mu}g/mL$ was 55% of untreated control. The assay has the potential to be used especially in the case that the primary cause of glyphosate resistance is related with a reduction of the herbicide translocation. Another assay is named 'leaf segment / shikimate accumulation assay', in which the four excised leaf segments ($4{\times}4mm$) are placed in each well of a 48-well microtiter plate containing $200{\mu}L$ test solution and the amount of shikimate is determined after incubation for 24 h in continuous light at $25^{\circ}C$. In this assay, 0.33% sucrose added to basic test solution enhanced a shikimate accumulation by 3 to 4 times and the shikimate accumulation was linearly occurred from 2 to $8{\mu}g/mL$ of glyphosate, showing an improved response to the method described by Shaner et al. (2005). The leaf segment / shikimate accumulation assay is simple and robust and has the potential to be used as a high throughput assay in the case that the primary cause of glyphosate resistance is related with EPSPS, target site of the herbicide. Taken together, these two assays would be highly useful to initially select the lines obtained after transformation, to investigate the migration of glyphosate-resistant gene into other weeds and to detect a weedy glyphosate-resistant corn in field.
Kim, Gun-Yeob;Song, Beom-Heon;Roh, Kee-An;Hong, Suk-Young;Ko, Byung-Gu;Shim, Kyo-Moon;So, Kyu-ho
Korean Journal of Soil Science and Fertilizer
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v.41
no.6
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pp.399-407
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2008
Importance of climate change and its impact on agriculture and environment has increased with a rise of greenhouse gases (GHGs) concentration in Earth's atmosphere, which had caused an increase of temperature in Earth. Greenhouse gas emissions such as methane($CH_4$) and nitrous oxide($N_2O$) in the field need to be assessed. GHGs fluxes using chamber systems in the fields(2004~2005) with pepper cultivation were monitored at the experimental plots of National Academy of Agricultural Science(NAAS), Rural Development Administration(RDA) located in Suwon city. $N_2O$ emission during pepper growing period was reduced to 74.0~82.1% in sandy loam soil compared with those in clay loam soil. Evaluating $N_2O$ emission at different levels of soil water conditions, $N_2O$ emission at -50 kPa were lowered to 13.2% in clay loam soil and 40.2% in sandy loam soil compared with those at -30 kPa. $CH_4$ emission was reduced to 45.7~61.6% in sandy loam soil compared with those in clay loam soil. Evaluating $CH_4$ at different levels of soil water conditions, $CH_4$ emission at -50 kPa was lowered to 69.6% in clay loam soil and 55.8% in sandy loam soil compared with those at -30 kPa. It implied that -50 kPa of soil water potential was effective for saving water and reducing GHG emissions. From the path analysis as to contribution factors for $N_2O$ emission, it appeared that contribution rate was in the order of mineral N(51.2%), soil temperature (25.8%), and soil moisture content(23.0%) in clay loam soil and soil moisture content(39.3%), soil temperature (36.4%), and mineral N(24.3%) in sandy loam soil.
BACKGROUND: High input to the arable land is contributed to increasing productivity with causing the global environmental problems at the same time. Rapeseed cultivation has been forced to reassess its positive point for utilization of winter fallow field. The Objective of this study was performed to assess the environmental impact of rapeseed cultivation with double-cropping system in paddy rice on Yeonggwang district using life cycle assessment technique. METHODS AND RESULTS: For assessing each stage of rapeseed cultivation, it was collected raw data for input materials as fertilizer and pesticide and energy consumption rate by analyzing the type of agricultural machinery and working hours by 1 ton rapeseed as functional unit. Environmental impacts were evaluated by using Eco-indicator 95 method for 8 impact categories. It was estimated that 216 kg $CO_2$-eq. for greenhouse gas, 3.98E-05 kg CFC-11-eq. for ozone lazer depletion, 1.78 kg SO2-eq. for acidification, 0.28 kg $PO_4$-eq. for eutrophication, 5.23E-03 kg Pb-eq. for heavy metals, 2.51E-05 kg B(a)p-eq. for carcinogens, 1.24 kg SPM-eq. for smog and 6,460 MJ LHV for energy resource are potentially emitted to produce 1 ton rapeseed during its whole cultivation period, respectively. It was considered that 90% of these potential came from chemical fertilizer. For the sensitivity analysis, by increasing the productivity of rapeseed by 1 ton per ha, potential environmental loading was reduced at 22%. CONCLUSION(s): Fertilization affected most dominantly to the environmental burden, originated from the preuse stage, i.e. fertilizer manufacturing and transporting. It should be included and assessed an indirect emission, which is not directly emitted from agricultural activities. Recycling resource in agriculture with reducing chemical fertilizer and breeding the high productive variety might be contribute to reduce the environmental loading for the rapeseed cultivation.
The aim of this study was to survey the perceptions of the elementary school teachers on the smallscale chemistry(SSC) following its training session. The teachers participating in the survey were 266 teachers in the Gyeongnam province. They were given a questionnaire that focused on the nine areas of the SSC: Needs for the teacher training and its application, its benefits, issues of safety and danger as well as treatment of environmental pollution, its economic efficiency and the development of investigative skills. The designed questionnaire was checked by an authority, and the responses to each question were tallied and analyzed. The results are as follows. The biggest problems of the traditional experimental methods as rated by the teachers were, in the order of importance, the preparation time, the legal liability of teachers for the safety and accidents, financial issues, disposal of the experimental wastes and the lack of relevant data. Since most of the teachers had not experienced the SSC lab programs in the field, they responded positively to the questions of need for its introduction and training. The implementation of the experimental SSC lab programs should proceed in the following order: introduction into the textbook, teacher training program, after-school education and the invitation of instructors. The most useful materials for the SSC program were CDs, videos, books and various printed materials, in that order. The responses regarding benefits of the SSC program included its simplicity, convenience, time savings, diversity, qualitative and quantitative aspects, integration into the regular class and use of toys. In particular, the teachers mentioned the increased safety due to the small amount of experimental reagents needed and the durability of plastic instruments. The familarity from the use of everyday tools as well as easy access to and the low-cost of the instruments were other important benefits. The teachers in general rated the educational content of the program highly, but many also found it to be average. Some pointed out the lack of sufficient discussion due to the individual or pair groupings as a potential shortcoming. The potential for development of problem solving ability and improvement of skills was rated positively. The number of teacher who rated the development of creativity positively was just over the half. As for the area of improving investigative skills, many found its assessment difficult and confusing because of the lack of its systemic definition and categorization. Based on the findings of this study, I would like to recommend the application and a wider dissemination of the small-scale chemistry lab program into the elementary school science curriculum.
This study was carried out to determine the promising method for the production of mother bulbs using garlic bulbs at the experimental field of Ange high school, Eusung in Korea from 1996 to 1998. The bulbs and bulbils were taken from a local cultivar 'Eusung' garlic. The growth of bulbil and yield potential were compared with different harvesting times and cultivation methods of bulbil. The harvesting time of bulbils which were capable of producing the mother bulbs was ranged from 15 days to 30 days after the emergence of flower stalks. The best results based on the yield and number of bulbs were obtained from the bulbils sampled at the just before garlic harvesting, 30 days after flower-stalk emergence. The mulching with transparent polyethylene(PE) film in bulbil cultivation was more effective for the plant growth and bulb formation than that with black PE film. The PE mulching from 30 days after seeding of bulbils to 30 days (late-April) before bulb harvesting was the moot effective for the increase of bulb yield. In the cultivation of garlic bulbils, application of lime (150 kg/10a) and compost (3,000 kg/10a) without any chemical fertilizers produced more bulbs in comparison with the standard level of fertilizer (N - $P_2O_5$ - $K_2O$ - compost - lime = 24.8 - 8.0 - 19.2 - 3,000 - 150 kg/10a). It was determined that the involucre seeding and assemble seeding of bulbils was effective and labour-saving methods for the production of mother bulbs from garlic bulbils. Input of labour force for the involucre seeding was decreased up to 61% as compared with the conventional drill seeding of bulbils. Mean yield from the cultivation of bulbil-derived single cloves was remarkably increased up to 95% in comparison with that of the common cloves. The larger size of single clove produced more yields, and the optimum planting density of single clove was $20{\times}10cm$. The cloves differentiated directly from bulbil showed to have higher yield potential than that from common cloves.
The possibility of future industrialization of artificial intelligence was studied through aspects of artificial intelligence art and movie. The field of artificial intelligence is developing by imitating humans through past and present, so it can be inferred that it is important to grasp the future image presented in movie and artificial intelligence art. Human values are represented differently in artificial intelligence films and arts. Artificial intelligence film and art are concerned with the external and internal aspects of human values, respectively. The AI movie looks at similar external aspects in human and AI shape and function, but artificial intelligence art deals with human alienation and lack of communication due to artificial intelligence technology development. Artificial intelligence in movies is a direction to visualize the imagination for artificial intelligence technology, and artificial intelligence art is expressed in the way of making and implementing works using technology. The future of artificial intelligence, which we have shown in imagination in movies today, is being realized technologically. Artificial intelligence art reflects the problems of artificial intelligence technology that can be appeared through current technology, and human problems that may arise from artificial intelligence technology development. Movies and artificial intelligence art reflect the current problems, and through them we can see the future of artificial intelligence. The future of artificial intelligence in movies is an artificial intelligence service that provides human convenience, cyborg artificial intelligence industry, industry that uses exoskeleton robot and exoskeleton suit, and artificial intelligence secretary. If we look at the future of artificial intelligence through the artificial intelligence art in terms of the problems of artificial intelligence technology and the problem of human value, there are artificial intelligence to learn from trial and error or mistakes, self-expression and communication by lifelogging, recovery of miscommunications by a reflective thinking, and an expansion of the area of artificial intelligence artist through human uncertainty. The future industrialization potential of artificial intelligence as study through aspects of artificial intelligence art and movie is an industry that extends the five senses, an industry that improves the insufficient physical ability of the human, an industry that enhances the physical ability of the human being, and an industry that maintains psychological and mental well-being.
Seo, Young-Jin;Kim, Jong-Su;Kim, Chang-Bae;Park, Man;Lee, Dong-Hoon;Choi, Choong-Lyeal;Choi, Jung
Korean Journal of Soil Science and Fertilizer
/
v.35
no.3
/
pp.162-168
/
2002
The silica uptake of rice plant(Oryza sativa L.) and a potential capacity of paddy soil as a source of silica supply for rice plant was studied under field experimental condition. Amount of Si from irrigation water, rainfall, which was a source of silica supply, and percolating rate of water through paddy soil profile as a source of silica loss from paddy soil, was investigated. Rice plants were grown in three different conditions, which included Control as non-fertilized, RDA's recommandation(NPK, Urea-Fused phophate-Potassium chloride=$110-45-57kg\;ha^{-1}$) and RDA's recommandation with supplement of silicate fertilizer(NPK+Si, Urea-Fused phosphate-Potassium chloride+Si=$110\;N-45\;P_2O_5-57\;K_2O+267.2\;Si\;kg\;ha^{-1}$). An amount of silica supply from rainfall was $0.5kg\;ha^{-1}$ and average amount of silica supplied from irrigation water $42.5kg\;ha^{-1}$, ranging from $28.1kg\;ha^{-1}$ to $58.8kg\;ha^{-1}$. Silica amount percolated through the soil profile have uniform trend comparatively showing $62.9kg\;ha^{-1}$ in Control, $64.8kg\;ha^{-1}$ in NPK treatment and $62.9kg\;ha^{-1}$ in NPK+Si treatment. Silica uptaked by Rice plant was $335.6kg\;ha^{-1}$ in Control, $406.6kg\;ha^{-1}$ in NPK+Si treatment and $471.1kg\;ha^{-1}$ in NPK+Si treatment. The difference between an amount of Si input(from rainfall, irrigation water and silicate fertilizer) and an amount of Si output(percolated Si in soil, uptaked Si by rice plant) was $357.4kg\;ha^{-1}$ in control, $412.1kg\;ha^{-1}$ in NPK treatment and $238.2kg\;ha^{-1}$ in NPK+Si treatment. Results of our study imply that paddy soil is a potential pool as a source of Si supply during growing periods of rice plant.
The cultivation of green manure crops plays an important role in soil quality and sustainability of agricultural system. However, the incorporation of green manure crops may be of concern because it can lead to strongly reducing conditions in the submerged soil. This study was conducted to evaluate the effects of rice husk carbon on rice (Oryza sativa L.) cultivation using green manure mixtures (hairy vetch + rye) in rice paddy. Field experiments were conducted in rice paddy soil (Shinheung series, fine loamy, mixed, nonacid, mesic family of Aeric Fluventic Haplaquepts) at the National Institute of Crop Science (NICS), Korea from October 2007 to October 2008. The experiments consisted of three treatments: application or no application of carbonized rice husk, and conventional fertilization. These treatments were subdivided into whole incorporation and aboveground removal of green manure mixtures. The redox potential (Eh) was higher upon application of the carbonized rice husk when compared to no application at 8 and 37 days after transplanting (DAT). The ammonium-N ($NH_4$-N) in soil was highest upon the application of carbonized rice husk + whole green manure incorporation at 17 and 49 DAT. Plant height and tiller number of rice were similar to the $NH_4$-N concentration in soil. Rice yields of application and no application of carbonized rice husk treatment were not significant. However, application of carbonized rice husk improved the soil physical properties such as bulk density and porosity after rice harvest. Therefore, the results of this study suggest that carbonized rice husk could be used as soil amendment for environmentally-friendly rice production under a green manure mixture-rice cropping system.
Ruth, Peter J. van;Nelson, Emma J.;Hillis, Richard R.
Geophysics and Geophysical Exploration
/
v.9
no.1
/
pp.50-59
/
2006
The risk of fault reactivation in the Gippsland Basin was calculated using the FAST (Fault Analysis Seal Technology) technique, which determines fault reactivation risk by estimating the increase in pore pressure required to cause reactivation within the present-day stress field. The stress regime in the Gippsland Basin is on the boundary between strike-slip and reverse faulting: maximum horizontal stress $({\sim}\;40.5\;Mpa/km)$ > vertical stress (21 Mpa/km) ${\sim}$ minimum horizontal stress (20 MPa/km). Pore pressure is hydrostatic above the Campanian Volcanics of the Golden Beach Subgroup. The NW-SE maximum horizontal stress orientation $(139^{\circ}N)$ determined herein is broadly consistent with previous estimates, and verifies a NW-SE maximum horizontal stress orientation in the Gippsland Basin. Fault reactivation risk in the Gippsland Basin was calculated using two fault strength scenarios; cohesionless faults $(C=0;{\mu}=0.65)$ and healed faults $(C=5.4;\;{\mu}=0.78)$. The orientations of faults with relatively high and relatively low reactivation potential are almost identical for healed and cohesionless fault strength scenarios. High-angle faults striking NE-SW are unlikely to reactivate in the current stress regime. High-angle faults oriented SSE-NNW and ENE-WSW have the highest fault reactivation risk. Additionally, low-angle faults (thrust faults) striking NE-SW have a relatively high risk of reactivation. The highest reactivation risk for optimally oriented faults corresponds to an estimated pore pressure increase (Delta-P) of 3.8 MPa $({\sim}548\;psi)$ for cohesionless faults and 15.6 MPa $({\sim}2262\;psi)$ for healed faults. The absolute values of pore pressure increase obtained from fault reactivation analysis presented in this paper are subject to large errors because of uncertainties in the geomechanical model (in situ stress and rock strength data). In particular, the maximum horizontal stress magnitude and fault strength data are poorly constrained. Therefore, fault reactivation analysis cannot be used to directly measure the maximum allowable pore pressure increase within a reservoir. We argue that fault reactivation analysis of this type can only be used for assessing the relative risk of fault reactivation and not to determine the maximum allowable pore pressure increase a fault can withstand prior to reactivation.
This research is to suggest an ecological management methods by classification of biotope type and its mapping for Tricholoma matsutake. The target area was construction site of the East Hongchun - Yangyang Highway (Yangyang section) where massive production of Tricholoma matsutakes is in place. Specifically, the 700 m long range of area around the road route, Yangyang section, which is over a total area of $19,79km^2$. The flow of this research was as follows: first studied were the characteristics of the Yangyang Tricholoma matsutakes; second, reviews on previous studies on Tricholoma matsutakes; third, the formulation of standards and processes of biotope type considering Tricholoma matsutake production; and fourth, mapping of biotope considering the production area of Tricholoma matsutake. In 24.77% of the region, slope angle measured $30{\sim}40^{\circ}C$, which is suitable for Tricholoma matsutake production. Also, 17.44% of the region had southward vegetation structures, and as for the Pinus densiflora; 26.00% had average receipts; the tree ages was $38{\pm}8.34$ years; and the average density of canopy layer was $9.55{\pm}4.89/100m^2$. The soil acidity (pH) values of 5.0 to 5.6(47.96%) > 5.6 to 7.0(42.90%) > 4.0 to 5.0(9.14%) were slightly high for Tricholoma matsutake growth; but 57.10% of the area had a soil pH of 4.0-5.6. The organic horizon (A0 layer) was $3.39{\pm}2.14cm$, and the mean depths were 4-6cm(78.03%) > 0-2cm(18.10%) > 2-4cm(3.87%). Based on the results of previous studies and field researches on the presence of vegetation (the pine dominance ratio), the topographic structure (the terrain characteristics and slopes), the receipt, the soil structure (the soil acidity and the organic layers interposed between the depths), and the vegetation density, six clusters based on the identified types of biotopes are produced in this paper. Area of distribution according to the type of biotope was Tricholoma matsutake of the potential-production (II) biotope (32.86%) > Tricholoma matsutake of the inadequate-production biotope (22.17%) > Tricholoma matsutake of the adequate (II)-production biotope (17.79%) > Tricholoma matsutake of the adequate (I)-production biotope (14.86%) > Tricholoma matsutake of the potential-production (I) biotope (9.77%) > Tricholoma matsutake of the produced biotope (2.55%).
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