• Reproductive and Developmental Biology
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• 제34권2호
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• pp.103-109
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• 2010

Functional regulation of a specific tissue or organ is controlled by a number of ways, including local cell-cell interaction. Of several forms of cell-cell junctional complexes, gap junctions are caught a great attention due to a formation of direct linkage between neighboring cells. Gap junctions are consisted of connexin (Cx) isoforms. In the present study, we evaluated expressional profiling of Cx isoforms in the rat initial segment (IS) of the male reproductive tract at different postnatal ages. The presence and expression of 13 Cx isoform mRNAs were determined by semi-quantitative real-time PCR analyses. A total of 8 Cx isoform mRNAs were detected in the IS of the male rats during postnatal development. The highest level of Cx30.3 mRNA was found at 5 months of age, while abundance of Cx31 mRNA was the highest at 1 year of age. Expression of Cx31.1 gene was relatively consistent during the postnatal development. Fluctuation of Cx32 and 37 gene expression was observed during the postnatal period. Significant elevation of Cx40 mRNA abundance was detected at 25 days of age and older ages. Expression patterns of Cx43 and 45 genes were similar with the highest level at 2 weeks of age, followed by gradual decreases at older ages. These results indicate differential regulation on expression of Cx isoforms in the rat IS during postnatal development. A complicated regulation of gene expression of Cx isoforms in the IS at different postnatal ages is suggested.

• Clinical and Experimental Pediatrics
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• 제65권1호
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• pp.1-9
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• 2022

With advancements in neonatal care and nutrition, the postnatal growth of preterm infants has improved; however, it remains an issue. Accurate assessments of growth using a standardized reference are needed to interpret the intrauterine and postnatal growth patterns of preterm infants. Growth in the earlier periods of life can contribute to later outcomes, and the refinement of postnatal growth failure is needed to optimize outcomes. Catch-up growth occurs mainly before discharge and until 24 months of age, and very low birth weight infants in Korea achieve retarded growth later in life. Knowing an infant's perinatal history, reducing morbidity rates during admission, and performing regular monitoring after discharge are required. Preterm infants with a lower birth weight or who were small for gestational age are at increased risk of poor neurodevelopmental outcomes. Furthermore, poor postnatal growth is predictive of adverse neurodevelopmental outcomes. Careful monitoring and early intervention will contribute to better development outcomes and national public health improvements.

• 가정과삶의질연구
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• 제25권5호
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• pp.167-183
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• 2007

The purpose of this study was to investigate the changes in infant-mother interactions and to measure infant-mother interaction, mutuality, and reciprocity at the 2nd and 6th postnatal monthperiods. Infant-mother interaction was assessed in the mornings, afternoons, and evenings in the 2nd and 6th postnatal months, by filming unstructured free play in the home and analyzing 28 of these infant-mother film recordingsusing two trained observers. This study can be summarized in five points. First, the separate frequency distribution of each category in MIIBR's maternal behavior was as follows: the frequencies of vocalization category, the kinesthetic exercise & physical contact category, the physical caregiving category, and theuse of play tool category. These all showed changes in the 6th postnatal month compared to the 2nd postnatal month. Second, the separate frequency distribution of each category in MIIBR's infant behavior was as follows: the frequencies of physical behavior category, the vocalization category, and the play behavior category. These also all showed changes in the 6th postnatal month compared to the 2nd postnatal month. Third, the mutuality and reciprocity of mother interaction decreased in the 2nd postnatal month compared to the 6th postnatal month. Fourth, mutuality and reciprocity in the infant interaction increased in 2nd postnatal month compared to the 6th postnatal month. Fifth, mutuality and reciprocity in the infant-mother interaction increased in 2nd postnatal month compared to the 6th postnatal month. In conclusion, it is evident that infant-mother interactions change over time. This study bears particular significance in setting a foundation for the theoretical basis of the interaction between infant and mother by observation.

• 환경생물
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• 제22권4호
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• pp.550-558
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• 2004

The effects of postnatal exposure to octylphenol(OP) on the expressions of the steroidogenic enzymes and testosterone production were evaluated. Postnatal male mice (15-day-old) were injected with 2 or 20mg $kg^{-l}$ body weight (BW) of OP for 5 days and sacrificed on postnatal day 21. Testosterone concentration was measured by radioimmunoassay and the expressions of the testicular genes were determined by RT-PCR analyses. Significant reductions in the mean body and testis weight were observed in the OP treated animals. No marked alteration in the histological structure of the testis were observed, however, slight reduction in the seminiferous tubule diameter and the number of Leydig cells and several pyknotic cells could be identified in the 20 mg $kg^{-l}$ BW of the OP treated animals. Serum testosterone concentration was dramatically reduced and the mRNA expressions of the steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (P450scc) and $17\beta$-hydroxylase/Cl7-20 lyase $(P450_{17\alpha})$ were decreased. No significant changes of the gene expressions of the steroidogenic factor-l (SF-I) and estrogen and androgen receptor after the OP treatment showed that the decreased expressions of the steroidogenic enzymes in the present study did not correlate with these genes. Altogether, the present study demonstrates that postnatal treatment of OP inhibits steroidogenesis by decreasing the transcriptional expressions of the StAR and steroidogenic enzymes. The alteration in steroidogenesis may adversely affect the normal development of the testis and sper- matogenesis.

• 한국발생생물학회지:발생과생식
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• 제22권1호
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• pp.65-72
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• 2018

Cyclic AMP-response element binding protein zhangfei (CREBZF), a member of ATF/CREB (activating transcription factor/ cAMP response element binding protein) family, regulates numerous cellular functions and development of cells by interacting transcription factors. This study discovered the expression pattern of CREBZF in seminiferous tubule of testes during the postnatal development of mice. In testis, CREBZF mRNA expression was the highest among other organs. Immunofluorescence analyses showed that the CREBZF was specifically expressed on spermatocyte but not in spermatogonia and Sertoli cells in seminiferous epithelium of mouse testis. Semi-quantitative polymerase chain reaction (PCR) analysis showed that CREBZF transcript level was significantly elevated during postnatal development of mouse testis. Confocal imaging analysis indicated that the protein expression of CREBZF in seminiferous tubule remained low until postnatal day (PD) 14, and was dramatically increased in PD 21. Interestingly, only one type of the spermatocyte expressed CREBZF specifically among SCP3-positive spermatocytes. Taken together, these results suggest that CREBZF may be novel putative marker of the spermatocyte and regulate meiosis during postnatal development of mice.

• 보건교육건강증진학회지
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• 제32권3호
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• pp.85-96
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• 2015

Objectives: This study aims to identify the influencing factors of using postnatal care among illiteracy women who live in the Democratic Republic of Congo. Methods: Household survey was done from February 1 to 8, 2013 in the Kwango district of Democratic Republic of Congo, and 400 childbearing women who has under 5 years old children and pregnant women was randomly selected and answered through the interview with a questionnaire. For analysis the data, ${\chi}^2$ test and logistic regression analysis were used. Results: Woman who can read, write and mathematical calculation was 195 (47.4%) of total 411 answers and 161 (39.2%) used postnatal care for their latest pregnancy. Age at first marriage (${\chi}^2=18.481$, p<.001), religions (${\chi}^2=11.165$, p=.011), languages (${\chi}^2=35.586$, p<.001), the experience of children death (${\chi}^2=16.507$, p<.001), antenatal care over 4 times (${\chi}^2=15.315$, p<.001), postnatal care (${\chi}^2=15.558$, p<.001) is significantly different from literacy level. Among illiterate women group, who are protestant (OR=.330), using Lingala (OR=.128), took elementary education (OR=.223) and farmer (OR=.040), used less postnatal care. Conclusions: For increasing usage of postnatal care among illiterate women, new approach method should be considered such as a visual communication method and a community health workers' training program for giving an outreach service to pregnant women care.

• Journal of Animal Science and Technology
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• 제51권6호
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• pp.493-502
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• 2009

Glucose is a major source of metabolic fuel and lipid and protein syntheses. Transport of glucose into the cell is regulated by an action of glucose transport.associated transporters, especially solute carriers 2A (Slc2a, protein symbol GLUT). The present study was focused on examination of mRNA expression of various Slc2a isoforms in the epididymis during postnatal development. Total RNAs isolated from different epididymal segments (caput, corpus, and caudal epididymis) were utilized for real-time polymerase chain reaction analyses. Results showed that Slc2a 1, 3, 4, 5, and 8 were expressed in the entire epididymal regions. In addition, the abundance of these Slc2a isoforms' transcripts was different within each epididymal regions. Moreover, the present study showed differential expression of these Slc2a isoforms among different epididymal segments according to postnatal ages. The current study suggests that glucose transport in the epididymis via various Slc2a isoforms would be necessary for maintenance of the epididymal functions.

• 생명과학회지
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• 제23권2호
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• pp.157-166
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• 2013

본 연구는 Sprague-Dawley계 숫쥐에서 출생 초기 에탄올에의 노출에 의한 성장 후 뇌혈류 자가조절의 변동을 관찰하고 이러한 변동에 대한 nociceptin의 관여를 관찰하고자 하였다. 실험동물에게 에탄올 2.5 g/kg을 생후 6, 7 및 8일의 3일 동안 2시간 간격으로 1일 2회 피하 주사하였다. 주령 4, 8 및 12주 시기에 단계적 출혈에 의한 저혈압 및 혈액 재주입에 의한 혈압 상승시의 평균동맥혈압의 변동에 따른 국소 뇌혈류 변동을 laser-Doppler flowmetry 방법으로 측정하였고, 경막과 대뇌피질에서 nociceptin-유사 면역반응력의 발현을 면역조직화학법으로 측정하였다. 출생 초기 에탄올 투여는 4, 8 및 12주령 모두에서 국소 뇌혈류 자가조절 기능을 거의 소실시켰다. 에탄올 투여 전에 nociceptin을 전처치한 군에서는 모든 연령군에서 국소 뇌혈류 자가조절 기능이 보존되었으나, nociceptin 수용체 선택적 경쟁적 길항제인 [$Nphe^1$]nociceptin(1-13)$NH_2$를 전처치한 군에서는 보존되지 아니하였다. 출생 초기 에탄올 투여에 의하여 경막 내 nociceptin-유사 면역반응력이 모든 연령군에서 현저히 증가하였고, 7-nitroindazole (7-NINA) 전처치뿐만 아니라 aminoguanidine 전처치에 의하여 모든 주령에서 유의하게 억제되었다. 출생 초기 에탄올 투여에 의하여 대뇌피질 내 nociceptin-유사 면역반응력이 모든 연령군에서 현저히 증가하였고, 7-NINA 전처치와 aminoguanidine 전처치에 의하여 모든 주령에서 유의하게 억제되었다. 모든 실험군의 동맥혈가스분석 결과는 실험 전, 중 및 후에 유의한 차이를 보이지 아니하였다. 이상의 결과로 보아 출생 초기 에탄올 투여는 성장 후 뇌혈류 자가조절에 변동을 초래하고, 이에 대한 보상기전으로서 nociceptin의 발현이 증가하는데, 여기에는 nitric oxide가 깊이 관여하는 것으로 생각된다.

• 대한생식의학회:학술대회논문집
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• 대한불임학회 2004년도 제46차 춘계학술대회
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• pp.59-59
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• 2004

• Neonatal Medicine
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• 제25권2호
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• pp.58-65
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• 2018

Purpose: The purpose of this study is to describe the rate of cytomegalovirus (CMV) virolactia, and the prevalence of breast milk (BM)-transmitted postnatal CMV infection among premature infants after freeze-thawing (FT) and Holder pasteurization (HP) of breast milk. Methods: This is a single-center, retrospective study of 312 infants born at less than 32 weeks of gestation, or with a birth weight less than 1,500 g from January 2013 to June 2017. All infants were screened for CMV-specific immunoglobulin (Ig) G and IgM at birth. Initial CMV specific polymerase chain reaction (PCR) and CMV culture were performed on mothers' BM and babies' urine within the first 21 days of life. FT and HP of BM was used to prevent the transmission of CMV. For the surveillance of postnatal CMV infection, CMV culture and CMV specific PCR of urine from babies were repeated one to two months after the initial screening. Screening for viremia and viruria was performed if postnatal CMV infection was suspected. Results: Among 178 BM samples obtained from mothers of CMV-IgG-seropositive infants, 80 (44.9%) were CMV PCR positive. CMV deoxyribonucleic acid (DNA) was detected in five of the 22 BM samples (22.7%) obtained from the mothers of CMV-IgG seronegative infants. When CMV DNA load in BM was measured before and after HP, various results were shown. Sixty-three infants out of 232 (27.2%) were evaluated for postnatal CMV infection and four infants out of 63 (6.3%) were infected. Conclusion: Interventions to prevent BM-transmitted CMV infection can reduce the chance of postnatal CMV infection, but not completely eliminate it.