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Effects of Postnatal Exposure to Octylphenol on the Transcriptions of Steroidogenic Enzymes in Mouse Testis  

Kim, Suel-Kee (Laboratory of Toxicogenomics for Endocrine Disruptors and Reproductive Endocrinology, Department of Life Science, Hanyang University)
Lee, Ho-Joon (Laboratory of Physiology, Department of Medicine, Eulji University School of Medicine)
An, Su-Yeon (Laboratory of Physiology, Department of Medicine, Eulji University School of Medicine)
Lee, Chang Joo (Laboratory of Toxicogenomics for Endocrine Disruptors and Reproductive Endocrinology, Department of Life Science, Hanyang University)
Yoon, Yong-Dal (Laboratory of Toxicogenomics for Endocrine Disruptors and Reproductive Endocrinology, Department of Life Science, Hanyang University)
Publication Information
Korean Journal of Environmental Biology / v.22, no.4, 2004 , pp. 550-558 More about this Journal
Abstract
The effects of postnatal exposure to octylphenol(OP) on the expressions of the steroidogenic enzymes and testosterone production were evaluated. Postnatal male mice (15-day-old) were injected with 2 or 20mg $kg^{-l}$ body weight (BW) of OP for 5 days and sacrificed on postnatal day 21. Testosterone concentration was measured by radioimmunoassay and the expressions of the testicular genes were determined by RT-PCR analyses. Significant reductions in the mean body and testis weight were observed in the OP treated animals. No marked alteration in the histological structure of the testis were observed, however, slight reduction in the seminiferous tubule diameter and the number of Leydig cells and several pyknotic cells could be identified in the 20 mg $kg^{-l}$ BW of the OP treated animals. Serum testosterone concentration was dramatically reduced and the mRNA expressions of the steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (P450scc) and $17\beta$-hydroxylase/Cl7-20 lyase $(P450_{17\alpha})$ were decreased. No significant changes of the gene expressions of the steroidogenic factor-l (SF-I) and estrogen and androgen receptor after the OP treatment showed that the decreased expressions of the steroidogenic enzymes in the present study did not correlate with these genes. Altogether, the present study demonstrates that postnatal treatment of OP inhibits steroidogenesis by decreasing the transcriptional expressions of the StAR and steroidogenic enzymes. The alteration in steroidogenesis may adversely affect the normal development of the testis and sper- matogenesis.
Keywords
octylphenol; steroidogenesis; testis; testosterone;
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1 Yoshida M, S Katsuda, A Takenaka, G Watanabe and K Taya, Maekawa A. 2001. Effects of neonatal exposure to a high-dose p-tert-octylphenol on the male reproductive tract in rats. Toxicol. Lett. 121:21-33   DOI   ScienceOn
2 Haavisto TE, A Adamsson, SA Myllymaki, J Toppari and J Paranko. 2003. Effects of 4-tert-octylphenol, 4-tertbutylphenol, and diethylstilbesterol on prenatal testosterone surge in the rat. Reprod. Toxicol. 17:593-605   DOI   ScienceOn
3 Ivanova T and C Beyer. 2000. Ontogenetic expression and sex differences of aromatase and estrogen receptor-$-\alpha/\beta$ mRNA in the mouse hippocampus. Cell Tissue Res. 300:231-237   DOI   ScienceOn
4 Majdic G, RM Sharpe and PTK Saunders. 1997. Maternal oestrogen/xenoestrogens exposure alters expression of steroidogenic factor-1 (SF-l/Ad4BP) in the fetal rat testis. Mol. Cell. Endocrinol. 127:91-98   DOI   ScienceOn
5 Nagoa T, Y Saito, K Usumi, M Kuwagata and K Imai. 1999. Reproductive function in rats exposed neonatally to bisphenol A and estradiol benzoate. Reprod. Toxicol. 13:303-311   DOI   ScienceOn
6 O'shayghnessy PJ, L Willerton and PJ Baker. 2002. Changes in Leydig cell gene expression during development in the mouse. BioI. Reprod. 66:966-975   DOI   ScienceOn
7 Pons S and I Torres-Aleman. 1993. Estradiol modulates insulin-like growth factor I receptors and binding proteins
8 Raychoudhury SS, CA Blake and CF Millette. 1999. Toxic effects of octylphenol on cultured rat spermatogenic cells and Sertoli cells. Toxicol. Appl. Pharmacol. 157: 192-202   DOI   ScienceOn
9 White R, S Jobling, SA Hoare, JP Sumpter and MG Parker. 1994. Environmentally persistent alkylphenolic compounds are estrogenic. Endocrinology 135:175-182   DOI   ScienceOn
10 Hess RA. 2003. Estrogen in the adult male reproductive tract: a review. Reprod. BioI. Endocrinol. 1:52   DOI   ScienceOn
11 Mueller SO. 2004. Xenoestrogens: mechanisms of action and detection methods. Anal. Bioanal. Chern. 378:582-587   DOI   PUBMED   ScienceOn
12 Toyama Y, I Hosoi, S Ichikawa, M Maruoka, E Yashiro, H Ito and S Yuasa. 2001. Beta-estradiol 3-benzoate affects spermatogenesis in the adult mouse. Mol. Cell. Endocrinol. 178:161-168   DOI   PUBMED   ScienceOn
13 Kuppers E and C Beyer. 1999. Expression of estrogen receptor$-\alpha$ and $\beta$ mRNA in the developing and adult mouse striatum. Neurosci. Lett. 276:95-98   DOI   ScienceOn
14 Wolkowicz MJ, SA Coonrod, PP Reddi, JL Millan, MC Hormann, JC Herr, SM Coonrod. 1996. Refinement of the differentiated phenotype of the spermatogenic cell line GC-2spd (ts). BioI. Reprod. 55:923-932   DOI   ScienceOn
15 Boockfor FR and CA Blake. 1997. Chronic administration of 4-tert-octylphenol to adult male rats causes shrinkage of the testes and male accessory sec organs, disrupts spermatogenesis, and increases the incidence of sperm deformities. BioI. Reprod. 57:267 -277   DOI   ScienceOn
16 Kuiper GGJM, JG Lemmen, B Carlsson, JC Corton, SH Safe, PT van der Saag, B van der Burg and JA Gustafsson.1998. Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor $\beta$. Endocrinology 139:4252-4263   DOI   ScienceOn
17 Peter M and JM Dubuis. 2000. Transcriptional factors as regulators of steroidogenic P450 enzymes. Eur. J. Clin. Invest. 30 (suppl. 3):14-20   DOI   ScienceOn
18 Manna PR, J Kero, M Tena-Sempere, P Pakarinen, DM Stocco and IT Huhtaniemi. 2001. Assessment of mechanisms of thyroid hormone action in mouse Leydig cells: Regulation of the steroidogenic acute regulatory protein, steroidogenesis, and luteinizing hormone receptor function. Endocrinology 142:319-331   DOI   PUBMED   ScienceOn
19 Sharpe RM, A Rivas, M Walker, C McKinnell and JS Fisher. 2003. Effects of neonatal treatment of rats with potent or weak (environmental) oestrogens, or with a GnRH antagonist, on Leydig cell development and function through puberty into adulthood. Int. J. Androl. 26:26-36   DOI   ScienceOn
20 Sharpe RM, JS Fisher, MM Millar, S Jobling and JP Sumpter. 1995. Gestational and lactational exposure of rats to xenoestrogens results in reduced testicular size and sperm production. Environ. Health Perspect. 103: 1136-1143   DOI   ScienceOn
21 Habert R, H Lejeune and JM Saez. 2001. Origin, differentiation and regulation of fetal and adult Leydig cells. Mol. Cell. Endocrinol. 179:47-74   DOI   PUBMED   ScienceOn
22 Guillette LJ Jr and MP Gunderson. 2001. Alterations in development of reproductive and endocrine systems of wildlife populations exposed to endocrine-disrupting contaminants. Reproduction 122:857-864   DOI
23 Nukula H, T Talonpoika, M Kaleva and J Toppari. 1999. Inhibition of hCG-stimulated steroidogenesis in cultured mouse Leydig tumor cells by bisphenol A and octylphenols. Toxicol. Appl. Pharmacol. 157:166-173   DOI   ScienceOn
24 Payne AH and GL Youngblood. 1995. Regulation of expression of steroidogenic enzymes in Leydig cells. BioI. Reprod. 52:217-225   DOI   ScienceOn
25 Gupta C. 1999. Modulation of androgen receptor (AR)mediated transcriptional activity by EGF in the developing mouse reproductive tract primary cells. Mol. Cell. Endocrinol. 152:169-178   DOI   PUBMED   ScienceOn
26 Murono EP, RC Derk and JH de Leon. 2000. Octylphenol inhibits testosterone biosynthesis by cultured precursor and immature Leydig cells from rat testes. Reprod. Toxicol. 14:275-288   DOI   ScienceOn
27 Murono EP, RC Derk and JH de Leon. 2001. Differential effects of octylphenol, $17\beta$-estradiol, endosulfan, or bisphenol A on the steroidogenic competence of cultured adult rat Leydig cells. Reprod. Toxicol. 15:551-560   DOI   ScienceOn
28 O'shaughnessy PJ and MA Mannan. 1994. Development of cytochrome P-450 side chain cleavage mRNA levels in neonatal ovaries of normal and hypogonadal (hpg) mice. Mol. Cell. Endocrinol. 104:133-138   DOI   ScienceOn
29 Ying GG, B Williams and R Kookana. 2002. Environmental fate of alkylphenols and alkylphenol ethoxylates - a review. Environ. Int. 28:215-226   DOI   ScienceOn
30 Blake CA and FR Boockfor. 1997. Chronic administration of the environmental pollutant 4-tert-octylphenol to adult male rats interferes with the secretion of luteinizing hormone, follicle-stimulating hormone, prolactin, and testosterone. BioI. Reprod. 57:255-266   DOI   ScienceOn
31 Nair-Menon JU, GT Campbell and CA Blake. 1996. Toxic effects of octylphenol on cultured rat and murine splenocytes. Toxicol. Appl. Pharmacol. 139:437-444   DOI   ScienceOn
32 Paris F, P Balaguer, B Terouanne, N Servant, C Lacoste, JP Cravedi, JC Nicolas and C Sultan. 2002. Phenylphenols, biphenols, bisphenol-A and 4-tert-octylphenol exhibit alpha and beta estrogen activities and antiandrogen activity in reporter cell lines. Mol. Cell. Endocrinol. 193:43-49   DOI   PUBMED   ScienceOn
33 $Str\ddot{o}mstedt$ M and MR Waterman. 1995. Messenger RNAs encoding steroidogenic enzymes are expressed in rodent brain. Mol. Brain Res. 34:75-88   DOI   ScienceOn
34 Majdic G, RM Sharpe, PJ O'shaughnessy and PTK Saunders. 1996. Expression of cytochrome P450 $17\beta-$hydroxylase/C 17-20 lyase in the fetal rat testis is reduced by maternal exposure to exogenous estrogens. Endocrinology 127:1063-1070
35 Kim SK, HJ Lee, HW Yang, HS Kim YD Yoon. 2004. Prepubertal exposure to 4-tert-octylphenol induces apoptosis of testicular germ cells in adult rat. . 50:1-15   DOI   ScienceOn
36 Nagao T, S Yoshimura, Y Saito, M Nakagomi, K Usumi and H Ono. 2001. Reproductive effects in male and female rats from neonatal exposure to p-octylphenol. Reprod. Toxicol. 15:683-692   DOI   ScienceOn