• Applied Microscopy
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• v.47 no.2
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• pp.63-69
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• 2017

Golgi staining has been modified and developed since Camillo Golgi introduced the black reaction in 1873. This study focuses on the commonly used Golgi staining methods and presents comprehensive data regarding three Golgi staining methods along with their strong and weak points. The Golgi-Cox method uses mercuric chloride for brain tissue impregnation and is a reliable technique for analyzing the complete dendritic tree of cortical neurons. However, specimens tend to shrink during the staining steps. Recent combination of the Golgi-Cox method and immunofluorescence provides additional options for neuroscientists. Rapid Golgi staining requires osmium tetroxide for the post-fixation process. It homogenously stains whole structures of neurons and provides their detailed anatomical morphology. This staining is influenced by the age of the specimen, temperature of the laboratory, and duration of each procedure. The Golgi-Kopsch method uses formaldehyde and glutaraldehyde instead of osmium tetroxide and can be used regardless of the age of the specimen and the duration after fixation. This method is suitable for research using human brain fixed for a long time or for specimens obtained from old-aged animals. Selecting a Golgi staining protocol that is appropriate for the specimen type and research purpose is important to achieve best results.

• Journal of Embryo Transfer
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• v.13 no.3
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• pp.219-226
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• 1998

Enucleation of oocytes is an important limiting step for embryo cloning. We propose an enucleation technique based on the removal of chromatin after oocyte activation by aspirating the second polar body containing complemented chromatin. In a preliminary experiment to determine an optimal age of oocytes enucleation in rabbits, oocytes were enucleated at 15~20 hours post hCG. Recently ovulated oocytes were enucleated at a higher rate than aged oocytes. Microsurgical removal of the complemented chromatin in the second polar body was significantly more effective in enucleating than aspiration of a larger cytoplasm volume surrounding the first polar body of metaphase-arrested oocytes(96.8% versus 70.4%; P〈0.05). Moreover, compared with a nuclear transplantation protocol based on enucleation of metaphase-arrested oocytes and preactivated oocytes followed by treatment with 5 $\mu$M ionomycin for 5 min and 2 mM DMAP for 1 hr, there was no significant difference in the rate of blastocyst development. The ease with which modified technique can be performed is likely to render this technique widely useful for research and practice on mammalian cloning.

• The Journal of Korean Physical Therapy
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• v.23 no.6
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• pp.15-22
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• 2011

Purpose: This research investigated falls due to obstacles that occur among elderly people by assessing changes in the values of plantar foot force, peak force, and plantar foot pressure in elderly subjects while they were stepping over obstacles of different heights. Methods: The subjects were 20 elderly people aged 70-80 years; Pressure was measured on flat ground(0 cm), and after installing obstacles of 8 cm and 12 cm using the F-scan system, which is a resistance-type pressure sensor. A one-way analysis of variance was performed to compare pressure on each part of the foot according to various heights after collecting data using the Tekscan program. The least significant difference test was used for the post-hoc analysis, A p-value <0.05 was considered significant. Results: The force value for the toe area (parts 1, and 2) and contact pressure increased significantly with the 12 cm obstacle (p<0.05). The peak force value and the peak contact pressure for part 1 increased significantly with the 12 cm obstacle (p<0.05). Conclusion: Larger changes appeared in the functions and structure of the foot while subjects walked over obstacles of different heights compared to flatland walking. This result suggests that people have safety strategies to prevent falls, and that there is a need for a more realistic approach through practice to overcome obstacles of various heights to prevent falls.

• Therapeutic Science for Rehabilitation
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• v.9 no.4
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• pp.21-31
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• 2020

Objective : Reminiscence program is a method to restore psychological stability for the elderly having dementia, and at the same time it makes the aged with dementia express themselves correctly by helping them to recollect their past life memories. The purpose of this study was to investigate the effects on communication ability in applying the reminiscence program to elderly patients with Alzheimer's dementia. Methods : The subject were 4 patients whose Alzheimer's dementia of moderate. This experiment was designed with pre-stage, treatment-stage, post-stage. The reminiscence therapy was compose of reminiscence activities of their live; in their childhood, adolescence, adulthood, and senescence. The therapy was delivered 30 times for 15 weeks. Results : The result of the study were as follows. First, after reminiscence therapy, recognition ability was improved. Second, after reminiscence therapy, emotional side was improved. Third, after reminiscence therapy, communication ability was improved. Conclusion : In this study, the reminiscence therapy had a positive effect on the improvement of communication skills among the elderly with Alzheimer's dementia. Based on the reminiscence therapy, it is thought to be very helpful in improving the communication ability of the elderly with dementia in the future.

• The Journal of Korean Medicine
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• v.37 no.2
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• pp.53-61
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• 2016

Objectives: Considering high prevalence of myopia in Asian countries, social cost paid for myopia may gradually increase in Korea. However, studies for developing myopia treatment are deficient. The study was to evaluate whether the periocular acupressure with a medical massager is effective for suppressing myopia progression. Methods: This study was an investigator-sponsored, prospective, open-labeled, and superiority pre and post single-armed study. 14 myopia children aged 7-12 years wore a medical massager for 15 minutes to stimulate periocular acupoints. For 24 weeks, the participants used the device twice a day. Based on prior studies, the refraction change of naturally proceeded myopia was set as -0.38 D. The axial length change of naturally proceeded myopia was set as 0.228 mm. To assess the safety, we performed vital sign check, physical examination, visual acuity test, slit lamp examination, IOP measurement, and fundus examination. Results: The refraction and axial length of the participants increased. For 24 weeks, the change in refraction was -0.38 D at the right eye and -0.40 D at the left. The change in axial length was 0.21 mm at the right and 0.22 mm at the left. In the safety assessment, any adverse event did not occur. Conclusions: There was no significant difference between the control group and the intervention group. The effect of growth may have more contributed to our result than that of acupressure. Based on our result, a full-scale study will not be conducted.

• Journal of Digital Convergence
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• v.16 no.10
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• pp.367-377
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• 2018

The purpose of the study is to verify the effectiveness the Preliminary Health Administrators(PHA)' Empathy Ability(EA) through the application of Medical Service Design Thinking(MSDT) conducted by undergraduate school of Intrapreneurship education. The pre-post questionnaire survey was conducted on 41 students in the second year of the Department of Health Administration after applying MSDT for 15 weeks from March to June, 2018 at a college in Daegu. The main results are as follows. MSDT was positive influenced on the improvement of Empathic Imagine, Empathic awakening of the PHA' EA. Especially, male student or aged 20-25, it was effective on the improvement of Empathic Imagine and Empathic awakening of the participants. Further research is needed on the development of MSDT programs for each grade to improve the empathy of PHA.

• The Journal of Korean Academy of Sensory Integration
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• v.15 no.1
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• pp.1-9
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• 2017

Objective : This paper is a report on the PLAY inSIde camp which connects sensory integration and play; the camp was designed for celebrating the KASI's 20th anniversary. Subjective : The camp was consisted of group play activity programs based on sensory integration for children aged 5~6 years with developmental disorder. The programs consisted of the Opening Program, Group Activity Program, Post Group Activity Program, and the Closing Activity Program. During the children's programs, their parents participated in parent education. The therapists participating in the camp planned and envisioned activity programs through pre-meetings and rehearsal sessions, where there were discussions among therapists and supporters. Conclusion : Through the camp, children experienced play activities involving sensory integration with other children, therapists shared their expertise in interaction with other therapists, and the KASI could share the accumulated resources with the society. After the events designed by the KASI, social participation through the occupation based on sensory integration could be facilitated and promoted in the future.

• Journal of Korean Academy of Nursing
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• v.48 no.2
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• pp.182-194
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• 2018

Purpose: This study aimed to estimate the effects of a health mentoring program on fasting blood sugar, total cholesterol, triglyceride, physical activity, self care behavior and social support changes among community-dwelling vulnerable elderly individuals with diabetes. Methods: A non-equivalent control group pre-post-test design was used. Participants were 70 community-dwelling vulnerable elderly individuals with diabetes. They were assigned to the experimental (n=30) or comparative (n=30) or control group (n=28). The experimental group participated in the health mentoring program, while the comparative group participated in health education program, the control group did not participate in any program. Data analyses involved a chi-square test, Fisher's exact test, a generalized linear model, and the Bonferroni correction, using SPSS 23.0. Results: Compared to the control group, the experimental and comparative groups showed a significant decrease in fasting blood sugar, total cholesterol, and triglyceride. Compared to the comparative and control groups, the experimental group showed significant improvement in self care behavior. However, there were no statistical differences in physical activity or social support among the three groups. Conclusion: These findings indicate that the health mentoring program is an effective intervention for community-dwelling vulnerable elderly individuals with diabetes. This program can be used as an efficient strategy for diabetes self-management within this population.

• Journal of Digital Convergence
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• v.15 no.2
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• pp.207-213
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• 2017

The study aimed to evaluate the effects of the magic program on the depression and self-esteem in rural low-income children. A quasi-experimental study employing a nonequivalent control group and pre-post design was conducted. The participants were children aged 9 to 12 years old from community child center in K province (Experimental group=24, Control group=21). The experimental group participated in the program for 8 sessions for 8 weeks. Data were analyzed using ${\chi}^2$-test, independent t-test, repeated measures ANCOVA with SPSS 21.0 program. There were significant differences in depression (F=6.68, p=.013) and self-esteem (F=7.61, p=.009) of participants between experimental group and control group after completion of 8-session magic program. The results indicate that the magic program can be used to improve depression and self-esteem of low-income children.

• Korean Journal of Health Education and Promotion
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• v.20 no.4
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• pp.79-93
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• 2003

Childhood injuries are the primary cause of death and disability among children aged 5 to 14. Consistent practice of learned safety behaviors can reduce the occurrence of severe injuries among children. However, safety behavior concern is low among Korean-American children specifically and American children, in general. The objective of the study is to evaluate the impact of an unintentional injury prevention curriculum, Risk Watch among Korean-American children. A quasi-experimental design with a nonequivalent control group was used for the designed of the study. Two intervention and two control Korean schools in Atlanta participated in this study. The intervention consisted of weekly lessons in traffic, bicycle, pedestrian, and fire safety. One hundred and two students completed a pre-test and a post-test. The main outcomes were safety behaviors (seat belt use or helmet use), behavioral intentions, and safety knowledge. Analysis of covariance was used for the statistical analyses. Strong intervention effects were found for increasing knowledge of all safety topics in the intervention group. Additionally, statistically significant intervention effects were detected for increasing seat belt and helmet use, as well as behavioral intentions of wearing a seat belt and wearing a helmet, among pre-kindergarten and kindergarten students. For students in grades 1 and 2, intervention effects were found for increasing helmet use. Among students in grades 3 to 8, the intervention group showed statistically significant increases for seat belt use. Limitations of the study and recommendations for modifying and supporting unintentional injury prevention programs for school children are discussed.