• Title/Summary/Keyword: Point light source

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Evaluation of SERS Nanoparticles to Detect Bacillus cereus and Bacillus thuringiensis

  • Hong, Jeehwa;Qin, Jianwei;Van Kessel, Jo Ann S.;Oh, Mirae;Dhakal, Sagar;Lee, Hoonsoo;Hwang, Chansong;Chan, Diane E.;Kim, Dongho;Cho, Hyunjeong;Kim, Moon S.
    • Journal of Biosystems Engineering
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    • v.43 no.4
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    • pp.394-400
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    • 2018
  • Purpose: This research evaluated five types of nanoparticles to develop a surface-enhanced Raman spectroscopy (SERS) method for the rapid detection of two Bacillus species (Bacillus cereus and Bacillus thuringiensis) that are commonly found on fresh produce, which can cause food poisoning. Methods: Bacterial concentrations were adjusted to a constant turbidity, and a total of $30{\mu}L$ of each Bacillus cell suspension was prepared for each nanoparticle. A point-scan Raman system with laser light source of wavelength 785 nm was used to obtain SERS data. Results: There was no qualitative difference in the SERS data of B. cereus and B. thuringiensis for any of the five nanoparticles. Three gold nanoparticles, stabilized in either citrate buffer or ethanol, showed subtle differences in Raman intensities of two Bacillus species at $877.7cm^{-1}$. Conclusions: Among the three types of nanoparticles, the gold nanoparticles stabilized in citrate buffer showed the lowest standard deviation, followed by gold nanoparticles stabilized in ethanol. This result supports the potential application of gold nanoparticles for SERS-based detection of B. cereus and B. thuringiensis.

A Melon Fruit Grading Machine Using a Miniature VIS/NIR Spectrometer: 2. Design Factors for Optimal Interactance Measurement Setup

  • Suh, Sang-Ryong;Lee, Kyeong-Hwan;Yu, Seung-Hwa;Shin, Hwa-Sun;Yoo, Soo-Nam;Choi, Yong-Soo
    • Journal of Biosystems Engineering
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    • v.37 no.3
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    • pp.177-183
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    • 2012
  • Purpose: In near infrared spectroscopy, interactance configuration of a light source and a spectrometer probe can provide more information regarding fruit internal attributes, compared to reflectance and transmittance configuration. However, there is no through study on the parameters of interactance measurement setup. The objective of this study was to investigate the effect of the parameters on the estimation of soluble solids content (SSC) and firmness of muskmelons. Methods: Melon samples were taken from greenhouses at three different harvesting seasons. The prediction models were developed at three distances of 2, 5, and 8 cm between the light source and the spectrometer probe, three measurement points of 2, 3, and 6 evenly distributed on each sample, and different number of fruit samples for calibration models. The performance of the models was compared. Results: In the test at the three distances, the best results were found at a 5 cm distance. The coefficient of determination ($R_{cv}{^2}$) values of the cross-validation were 0.717 (standard error of prediction, SEP=$1.16^{\circ}Brix$) and 0.504 (SEP=4.31 N) for the estimation of SSC and firmness, respectively. The minimum measurement point required to fully represent the spectral characteristics of each fruit sample was 3. The highest $R_{cv}{^2}$ values were 0.736 (SEP=$0.87^{\circ}Brix$) and 0.644 (SEP=4.16 N) for the estimation of SSC and firmness, respectively. The performance of the models began to be saturated when 60 fruit samples were used for developing calibration models. The highest $R_{cv}{^2}$ of 0.713 (SEP=$0.88^{\circ}Brix$) and 0.750 (SEP=3.30 N) for the estimation of SSC and firmness, respectively, were achieved. Conclusions: The performance of the prediction models was quite different according to the condition of interactance measurement setup. In designing a fruit grading machine with interactance configuration, the parameters for interactance measurement setup should be chosen carefully.

Stylized Specular Reflections Using Projective Textures based on Principal Curvature Analysis (주곡률 해석 기반의 투영 텍스처를 이용한 스타일 반사 효과)

  • Lee, Hwan-Jik;Choi, Jung-Ju
    • Journal of the HCI Society of Korea
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    • v.1 no.1
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    • pp.37-44
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    • 2006
  • Specular reflections provide the visual feedback that describes the material type of an object, its local shape, and lighting environment. In photorealistic rendering, there have been a number of research available to render specular reflections effectively based on a local reflection model. In traditional cel animations and cartoons, specular reflections plays important role in representing artistic intentions for an object and its related environment reflections, so the shapes of highlights are quite stylistic. In this paper, we present a method to render and control stylized specular reflections using projective textures based on principal curvature analysis. Specifying a texture as a pattern of a highlight and projecting the texture on the specular region of a given 3D model, we can obtain a stylized representation of specular reflections. For a given polygonal model, a view point, and a light source, we first find the maximum specular intensity point, and then locate the texture projector along the line parallel to the normal vector and passing through the point. The orientation of the projector is determined by the principal directions at the point. Finally, the size of the projection frustum is determined by the principal curvatures corresponding to the principal directions. The proposed method can control the position, orientation, and size of the specular reflection efficiently by translating the projector along the principal directions, rotating the projector about the normal vector, and scaling the principal curvatures, respectively. The method is be applicable to real-time applications such as cartoon style 3D games. We implement the method by Microsoft DirectX 9.0c SDK and programmable vertex/pixel shaders on Nvidia GeForce FX 7800 graphics subsystems. According to our experimental results, we can render and control the stylized specular reflections for a 3D model of several ten thousands of triangles in real-time.

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The Developed Study for SMPS to Protect the Noise and Inrush Current at LED Lighting Source (LED 광원에서 잡음 및 돌입전류 방지를 위한 스위칭모드 전원공급 장치 (SMPS) 개발 연구)

  • Chung, Chansoo;Hong, Gyujang;We, Sungbok;Yu, Geonsu;Kim, Mijin
    • KEPCO Journal on Electric Power and Energy
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    • v.2 no.4
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    • pp.577-582
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    • 2016
  • This Study focused on the development of SMPS (Switching Mode Power Supply) to supply the constant votage and current nevertheless LED fluorescent Light generated the electric noise (with Harmonics) and Inrush current at instant time of turn-on and off. Recently, according to the Green policy in government, the LED fluorescent Lighter showed the rapidly increasing tend as indoor and outdoor Lighter. But, because of costs, LED fluorescent Light not considered and neglected the following items; power factor, efficiency, Harmonics and Inrush current. So, we are developed the SMPS about 3 key issues as follows: 1st, power factor and efficiency is 85%. 2nd, the switching noisy by harmonic is minimized. 3rd, the Inrush current at turn on and off time is reduced the minimum 0.3 A after $100{\mu}sec$ on turnon time. The proposed SMPS adjusted by LNK 409 driver (included the high frequency modulation function). Although, the developed SMPS maintained the about 85% of power factor and efficiency. but, the SMPS must be generated low heat by the variation of minute load current at switching timing. To improve the above weak point, the developed SMPS have the feedback monitoring circuit between input side and output side to maintain the power factor and efficiency. Also, we are studied the time-constant of control circuit to output the constant voltage and current nevertheless the load disturbance of LED lighting. The LED fluorescent Light of 46W is checked the above items.

An Evaluation on Citizens' Satisfaction with the Outdoor Landscape Lighting in Gyeongju Historic Areas - The Case of Wolseong District -

  • Park, So-Yeong;Heo, Sang-Hyun
    • Journal of the Korean Institute of Landscape Architecture
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    • v.38 no.5_2
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    • pp.186-193
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    • 2010
  • The purpose of this study was to survey the citizens for their satisfaction with the outdoor landscape lighting in Gyeongju Historic Areas registered as UNESCO World Cultural Heritage in November 2000 and thereupon, provide for some basic data useful to the design of the outdoor landscape lighting for the cultural properties. As a result of examining the conditions of the outdoor landscape lighting in Wolseong Zone of Anapji, Banwolseong, Dongbu Sajeokji, Cheomseongdae and Gyerim, there were found 391 lighting fixtures of 12 types in Anapji, 138 lightings of 4 types in Banwolseong, 38 lightings of 6 types in Cheomseongdae, 28 lightings of 3 types in Dongbu Sajeokji and 54 lightings of 5 types in Gyerim. As a result of analyzing citizens' satisfaction with the outdoor landscape lighting, it was found that citizens were satisfied more or less with the nightscape image changed by the outdoor landscape lighting; their satisfaction scored 3.836 on average for Anapji on a 5-point Likert type scale, 3.516 for Banwolseong, 3.446 for Dongbu Sajeokji, 3.650 for Cheomseongdae and 3.479 for Gyerim. However, citizens' satisfaction with the originality of the nightscape was generally low: 3.055 for Anapji, 2.914 for Cheomseongdae, 2.877 for Banwolseong, 2.847 for Gyerim and 2.665 for Dongbu Sajeokji. On the other hand, since most of the lighting fixtures were installed as inserted lights or floodlights, the color tones of light source were relatively highly distinctive, but the peripheral spaces around the cultural properties were rather dark, which means that citizens were feeling inconvenient more or less for using the amenities such as bench or waste box. All in all, their satisfaction with the outdoor landscape at the sample zone at night scored 2.981, lower than the normal level.

A Study on the Heroine's Costumes in the Movie Titled ${\ulcorner}$YMCA Baseball Team${\lrcorner}$ - By Focusing on the Costumes When the Country Opened Itself to the Outside World - (영화 "YMCA 야구단"에 나타난 여 주인공의 의상 디자인 연구 - 개화기 시대의 의상 비교를 중심으로 -)

  • Kim, Min-Ji;Byun, Mi-Yeon;Lee, In-Seong
    • The Research Journal of the Costume Culture
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    • v.14 no.5
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    • pp.728-738
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    • 2006
  • As movies and dramas which show traditional clothes and fusion clothes are getting popular these days, lot of people are paying attention to these dress and ornaments. And as for the domestic collections which seem to reflect this trend, various fusion style clothes are getting widely adopted by people. Therefore, this study analyzed and considered the clothes designs when the country started to open itself to other countries, by selecting a movie titled "YMCA baseball team", a movie to ascertain vigorous historical verification, and by conducting the prior study and using the DVD capture program. This study is meaningful in that based on the comparison study on the costumes, it clarifies the degree of costumes(clothes) acceptance at the point of nation's opening to other countries, which is the source of fusion style, and further shed light on the role that the movie costumes will play. From this study, we can get the following conclusions. First, through the theoretical background it can be known that when the country opened itself to outside world the dress and ornaments of the time was adopted in a top-down way, namely, first adopted by the royal family and the upper class, which could have easy access to the western civilization and institutions, and then the low class later. Second, in the movie titled 'YMCA baseball team' the costumes were designed through the strict and thorough historic verification, and it can be confirmed that various dress and ornaments adopted at the time of nation's opening to the outside world centered around the clothes design for new women. Third, 'YMCA baseball team is evaluated as a movie that reproduces that time well and as a media that provides an effective clue to the dress and ornaments adopted by the public when the country opened itself to the outside world.

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Imaging of self-assembled monolayers by surface plasmon microscope (표면 플라즈몬 현미경을 이용한 자기조립 단분자막의 이미징)

  • 표현봉;신용범;윤현철;양해식;김윤태
    • Korean Journal of Optics and Photonics
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    • v.14 no.1
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    • pp.97-102
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    • 2003
  • Multi-channel images of 11-MUA(11-Mercaptoundecanoic acid) and 11-MUOH(11-Mercaptoundecanol) self-assembled monolayers were obtained by using two-dimensional surface plasmon resonance (SPR) absorption. The patterning process was simplified by exploiting direct photo-oxidation of thiol bonding (photolysis) instead of conventional photolithography. Sharper images were resolved by using a white light source in combination with a narrow bandpass filter in the visible region, minimizing the diffraction patterns on the images. The line profile calibration of the image contrast caused by different resonance conditions at each point on the sensor surface (at a fixed incident angle) enables us to discriminate the monolayer thickness in nanometer scale. Furthermore, there is no signal degradation such as photo bleaching or quenching, which are common in the detection methods based on fluorescence.

A Study on the Establishment of a Production Pipeline Imported 3D Computer Graphics for Clay Characters (3D 컴퓨터그래픽을 도입한 클레이 캐릭터 제작 공정 개발에 관한 연구)

  • Kim, Jung-Ho
    • Journal of Korea Multimedia Society
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    • v.11 no.9
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    • pp.1245-1257
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    • 2008
  • The establishment of a production pipeline imported 30 computer graphics is suggested in this paper to improve the efficiency of existing production pipeline of clay animation. The point is that the process of building clay characters that remains labor intensive among the existing procedures is replaced by the process of creating computer generated characters. In order to create characters out of clay by means of 30 computer graphics, a diffuse map and displacement map are made of an oil-based clay according to the UVW coordination of polygon modeling, which is the same color and kind of clay used to make a clay character. In addition, a panoramic HDRI recording system is developed to record the lighting information of shooting environment for miniature sets, which is imported in 3D computer graphic tools as digital light source. On account of the new production pipeline, a hyper realistic rendering image can be produced, and at the same time it improves the traditional pipeline of stop motion animation that is know-how based procedure of a complete artist by the engineering approach to the automatic process.

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A Study on the Preparation of Tritium Luminous Compound

  • Kim, Jaerok
    • Nuclear Engineering and Technology
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    • v.4 no.4
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    • pp.331-339
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    • 1972
  • For the syntheses of tritium labelled polystyrene, the basic material for the preparation of tritium luminous compound, various methods of labelling such as Tesla discharge, Wilzbach exposure, gamma irradiation, and U V. irradiation were compared in view of getting high specific activity-product. The obtained polystyrene-T(G) by the method of Tesla discharge and by the method of U. V. irradiation had specific activity of 1~1.2 mCi/mg, and these two methods were the most encouraging. Mixing of 1 part of polystyrene-T(G) with 4 parts of ZnS:Cu phosphor, in weight, appeared to be the most suitable ratio in tile preparation of luminous compound in luminosity point of view. When 30 mg. of obtained luminescent mixture was applied on steel plate by using 1 ml. of the selected binder (i.e., 1g of commercial varnish in 100m1. of benzene) the luminosity maximum was ca. 20 micro Lambert. The prepared luminous compound was confirmed to be practically applicable for mine marker or dark-room light source.

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"COVID-19 : Our Memory" : A Digital Archive for Social Changes caused by SARS-CoV-2 ("코로나-19 : 우리의 기억" : 코로나바이러스 감염증과 사회변화에 대한 디지털 아카이브)

  • Kim, Haklae
    • Journal of Korean Society of Archives and Records Management
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    • v.20 no.4
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    • pp.229-236
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    • 2020
  • In light of SARS-CoV-2's significant impact, human society has experienced rapid changes in lifestyle that it has not yet experienced before. One way this virus has influenced people's lives is the emergence of the zero-contact society, an initiative for preventing the spread of infectious diseases. As can be seen, the social impact of COVID-19 is widespread. Various issues, such as those about government policy, personal information protection, and health care, are affecting society as a whole. At the same time, factual information is difficult to track and record because of the rapid and transient nature of related events and issues. As such, a method of effectively describing COVID-19 and real-time information is necessary. The "COVID-19: Our Memory" project is an attempt to record the sociocultural impact of the coronavirus infection. This project collects major events and issues classified into several subjects, records those events from a neutral point of view, and develops a digital archive so that all records are accessible. All the data collected and built through the project, the application, including the source code and visualization, are all published to bring about new opportunities for collaboration.