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http://dx.doi.org/10.3807/KJOP.2003.14.1.097

Imaging of self-assembled monolayers by surface plasmon microscope  

표현봉 (한국전자통신연구원 반도체 원천기술연구소)
신용범 (한국전자통신연구원 반도체 원천기술연구소)
윤현철 (한국전자통신연구원 반도체 원천기술연구소)
양해식 (한국전자통신연구원 반도체 원천기술연구소)
김윤태 (한국전자통신연구원 반도체 원천기술연구소)
Publication Information
Korean Journal of Optics and Photonics / v.14, no.1, 2003 , pp. 97-102 More about this Journal
Abstract
Multi-channel images of 11-MUA(11-Mercaptoundecanoic acid) and 11-MUOH(11-Mercaptoundecanol) self-assembled monolayers were obtained by using two-dimensional surface plasmon resonance (SPR) absorption. The patterning process was simplified by exploiting direct photo-oxidation of thiol bonding (photolysis) instead of conventional photolithography. Sharper images were resolved by using a white light source in combination with a narrow bandpass filter in the visible region, minimizing the diffraction patterns on the images. The line profile calibration of the image contrast caused by different resonance conditions at each point on the sensor surface (at a fixed incident angle) enables us to discriminate the monolayer thickness in nanometer scale. Furthermore, there is no signal degradation such as photo bleaching or quenching, which are common in the detection methods based on fluorescence.
Keywords
surface plasmon resonance; self-assembled monolayer; protein chip; imaging system;
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