• 대한약침학회지
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• 제4권3호
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• pp.59-67
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• 2001

Objective: The purpose of this report is to prove the clinical effect of Platycodon grandiflorum aqueous extract on pneumoniae patients. Methods: We used the aqueous extract of Platycodon grandiflorum to treat two pneumoniae patients. It was injected into five acupuncture points, which was Chondol(天突:CV22) 1 point, Pyesu(肺兪 : BL13) 2 point, and Kworumsu(厥陰兪: BL14) 2 point. Results & conclusions: We have used the aqueous extract of 24-year-old JK for treating the patients suffering from lung diseases, and have experienced the actual effects. Of the treated, two pneumonia-involved patients showed apparent improvement in simple chest X-ray and clinical symptoms. The patients were treated with JK (Jang-saeng platycodon) aqueous extract 25 and 22 times individually. The results were as follows. 1. The symptoms including coughing, phlegm, and fever were improved in two cases. 2. The lung infiltration in simple chest X-ray decreased and the WBC count was kept within normal range in two cases. 3. Side effect such as itching was not found in the process of JK aqueous extract treatment. 4. The criteria for pneumonia are fever, coughing with purulent phlegm, pleural chest pain, the evidence of new infiltration in simple chest X-ray, sign of lung sclerosis in auscultation, increase of WBC count, etc. But they may not be the proper objective diagnostic standards. So we had trouble in statistic process and numerical interpretation. Putting these results together, the JK aqueous extract is considered to be effective in treating patients for pneumonia, and the continuous research and accumulation of data is needed.

• 한국약용작물학회지
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• 제18권4호
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• pp.273-279
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• 2010

The study result survey platycodon cultivation regions of Jinju(2), Sacheon(4), Hapcheon(3), Haman(3), and Sancheong(3) of Gyongnam regarding chemical properties of soil, inorganic nitrogen phosphorus by type of composition is as follows: pH and EC value were highest where platycodon cultivated for under 3 years in soils. Content of O.M equal to or less than 25 mg $kg^{-1}$, the average value for platycodoncultivation in korea. Content of exchangeable calcium in soils were 12, 14 $cmol^+kg^{-1}$ in Hapcheon and Haman regions where platycodon was cultivated for two years which is 2~3 time higher level compared with the average upland soil of korea. Water solution boron confirmed approximated level of 3.0 mg $kg^{-1}$ in all survey regions, and the finding is about five times higher than average upland soil of korea which is 0.5 mg $kg^{-1}$. In the case of nitrogen, a significant difference was found depending on cultivation regions and continuous culture. Content of nitrogen in soils were difference according to cultivation area and continuous culture. The composition ratio according to the type of inorganic phosphorus showed the highest in order of Al-P > Ca-P > Fe-P > Saloid-P in all survey regions except for Hapcheon (five, seven years cultivated soils). The correlation showed high significance between available phosphate and inorganic phosphorus.

• 한국약용작물학회지
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• 제10권3호
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• pp.200-205
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• 2002

길경의 부탄올 분획으로부터 지표성분을 분리, 정제하기 위해 $SiO_2$ 컬럼크마토그래피를 실시한 결과 1종(화합물 1)의 단일 물질을 순수 분리하였으며 화합물 1의 구조는 $^1H$, $^{13}C$ NMR 등 분광학적 관측 결과 platycodin D로 확인되었다. Platycodin D의 HPLC 정량법을 검토한 결과, 검출기는 증기화광산란검출기(ELSD)를, 컬럼은 역상컬럼을 사용하여 길경 MeOH 추출액으로부터 효율적인 지표성분 정량이 가능하였다. 추출조건을 재검토한 결과 환류추출보다는 초음파 추출이 더 효율적이었으며 추출용매로는 80% MeOH이 다른 용매조건보다 추출효율이 우수하였다. 건조조건의 검토에서는 platycodin D의 함량이 양건이나 양건과 건조기 혼용건조 보다 건조기 단독 건조시 더 높았으며 특히 $60^{\circ}C$ 열풍건조에서 그함량이 0.083%로서 가장 높았다. 묽은 에탄올 엑스 함량도 건조기만을 이용한 열풍건조가 양건과 건조기 열풍건조를 혼용한 방법보다 4% 정도 더 높았다.

• Applied Biological Chemistry
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• 제40권2호
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• pp.152-156
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• 1997

도라지는 약리성분을 많이 함유하고 있으며 식품 및 생약재료로 널리 사용되고 있다. 재배도라지 24년근 및 3년근의 주요성분을 동정하기 위하여 약리성분을 분석하고 비교하였다. 재배도라지의 에탄올 추출물에서는 24년근에서 역상column을 사용한 HPLC분석에서 retention time이 약 50분 이후부터 3년근에는 보이지 않았던 peak가 나타나고 있어 연수에 따라 다수의 약리성 물질이 생성되거나 미량물질이 생성되었다. 재배도라지의 sterol 조성비 및 saponin 함량에서는 24년근과 3년근간에는 별다른 차이가 없었다. 재배도라지 15년근및23년근, 인삼6년근, 더덕3년근 모두 유의성있는 항보체 활성효과는 없었다.

• 한국식품과학회지
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• 제33권6호
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• pp.651-655
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• 2001

장생 도라지의 메탄올 추출물과 분획물들의 돌연변이 유발 억제 효과를 조사하기 위하여 IQ와 MNNG를 사용하여 Ames mutagenicity test를 검토하였다. 장생 도라지 메탄올 추출물의 돌연변이원성은 없었으며, 또한 메탄올 추출물의 농도 증가에 따른 돌연변이 억제 효과는 상당히 높게 나타났다. 그리고 메탄올 추출물의 분획물중 에틸아세테이트층과 부탄올층에서 돌연변이 억제 효과가 인정되었으며, 농도 증가에 따라 colony 수가 감소하였다. S. typhimurium TA98과 TA100 균주와 각 돌연변이 물질에 따라 돌연변이 억제효과는 다소간의 차이가 있었으나, 장생 도라지의 약리 성분은 대사 활성화 과정의 돌연변이 억제 효과뿐만 아니라, 체내 세포에 직접적으로 영향을 미치는 직접돌연변이원에 대해서도 억제효과가 관찰되었다.

• Natural Product Sciences
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• 제10권4호
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• pp.141-151
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• 2004

Platycodi Radix, as one of the most important traditional oriental materia medica, is utilized frequently in clinical practice and dietary in China, Korea, and Japan. To evaluate definitely the quality of Platycodi Radix, and provide scientific data for commodity circulation, new product development and clinical application of Platycodi Radix, we summarized its medical history, application status, chemical compositions, bioactivities, cultivation, international circulation, quality evaluation, dosage and safety, and brought forward some constructive suggestions on further establishment of international criteria for quality control of Platycodon grandiflorum.

• Journal of Plant Biology
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• 제38권4호
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• pp.337-341
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• 1995

Hairy roots of Korean ballon flower (Platycodon grandiflorum A. DC) were induced from the root tissues infected with Agrobacterium rhizogenes ATCC 15834. Growth and polyacetylene [lobetyol (1), lobetyolin (2) and lobetyolinin (3)] production fo ten hairy root clones cultured in 1/4 Gamborg B5 (B5) liquid medium were determined. One selected hairy root clone (D6) grew well in hormone free-B5 liquid medium and showed maximum content of polyacetylenes at week 6 for 1 (0.375% dry wt) and at week 7 for 2 and 3 (3.030% and 0.206% dry wt, respectively) whose levels were much higher than those of the intact plant root (1:0.019%, 2:0.077% dry wt, 3 was not detected).

• Journal of Microbiology and Biotechnology
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• 제19권6호
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• pp.556-559
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• 2009

Compounds extracted from Platycodon grandiflorum were evaluated for an activation effect on nuclear factor-kappa B (NF-${\kappa}B$). In its active state, NF-${\kappa}B$ turns on the expression of genes related to cell proliferation or death. NF-${\kappa}B$ activators promote growth of neuron cells and can be used to control neurodegenerative diseases. The biological activity of P. grandiflorum extracts toward NF-${\kappa}B$ had not yet been studied. Although the biological activity of several compounds extracted from P. grandiflorum was evaluated, only three exhibited any significant activation effect on NF-${\kappa}B$.

• 한국약용작물학회지
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• 제19권4호
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• pp.233-237
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• 2011

This study was carried out to investigate the seedling establishment and yield of direct-sown Platycodon grandiflorus seeds cultured by three ridge width, 0.4, 0.8, and 1.2 m and four mulching materials, rice straw, rice bran, black plastic film, and no mulching in upland. Seedling establishment rate (70%) was the highest in rice straw mulched plot of 500 seeds/$m^2$ sowed and root yield after one year was also the highest in rice straw mulched treatment and followed by rice bran, no mulching, and black plastic film treatment. In this result, ridge width 120cm and rice straw mulching combined treatment was best for getting the highest seedling establishment ratio and seedling numbers per area. However, ridge width and mulching materials should be considered soil moisture content and weed population for saving labor cost.

• 생약학회지
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• 제42권3호
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• pp.213-217
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• 2011

Platycodon grandiflorum (Campanulaceae) is listed in Shin-Nong-Bon-Cho-Kyung (神農本草經) as the remedy for ventilation the lung, resolving phlegm, promoting pus discharge and subduing carbuncles. The prescriptions of Gil-Gyung-Tang (桔梗湯) are also recorded in many other Chinese medicinal books. The identification of the cultivation year of P. grandiflorum is very important in commercial market as well as in research field. However, any reports about it have not been clearly established yet. To clarify the criterion, the morphological and anatomical characteristics of the roots of P. grandiflorum cultivated in Korea for various years were studied. The characteristics of the cork layer, latex tube, and vessels were shown to be useful keys to confirm the cultivation year of P. grandiflorm. As a result, it was clarified that Kilkyung from Korea was the two years root of Platycodon grandiflorm.