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Quantitative Analysis of Platycodin D from Platycodon grandiflorum by HPLC-ELSD  

Kim, Geum-Soog (Industrial Crop Division, National Crop Experiment Station, RDA.)
Kim, Hyun-Tae (Upland Crops Division, National Yeongnam Agricultural Experiment Station, RDA.)
Seong, Jae-Duck (Upland Crops Division, National Yeongnam Agricultural Experiment Station, RDA.)
Park, Hee-Saeng (Upland Crops Division, National Yeongnam Agricultural Experiment Station, RDA.)
Kim, Soo-Dong (Upland Crops Division, National Yeongnam Agricultural Experiment Station, RDA.)
Publication Information
Korean Journal of Medicinal Crop Science / v.10, no.3, 2002 , pp. 200-205 More about this Journal
Abstract
Platycodin D was isolated from n-butanol extract of Platycodi radix(Platycodon grandiflorum and identified by the spectroscopic analysis using $^1H$ and $^{13}C$ NMR techniques. A new method of analysis of platycodin D by high performance liquid chromatography(HPLC) was established using a reversed phase system with YMC-Pack ODS-AM( 250 X 4.6 mm) column and 30% acetonitrile as a mobile phase. Evaporative light scattering detector was used as detector. The kinds of extraction solvents and methods were examined to determine the efficient extraction condition and HPLC analysis was carried out to establish the optimum drying condition for the root of Platycodon grandiflorum. The contents of Platycodin D was highest as 0.083% when platycodon roots were dried at $60^{\circ}C$ using dry oven.
Keywords
Platycodon grandiflorum; platycodin D; HPLC; ELSD; Drying condition;
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