Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Quantitative Analysis of Platycodin D from Platycodon grandiflorum by HPLC-ELSD

HPLC-ELSD법에 의한 길경의 platycodin D 정량분석

  • Kim, Geum-Soog (Industrial Crop Division, National Crop Experiment Station, RDA.) ;
  • Kim, Hyun-Tae (Upland Crops Division, National Yeongnam Agricultural Experiment Station, RDA.) ;
  • Seong, Jae-Duck (Upland Crops Division, National Yeongnam Agricultural Experiment Station, RDA.) ;
  • Park, Hee-Saeng (Upland Crops Division, National Yeongnam Agricultural Experiment Station, RDA.) ;
  • Kim, Soo-Dong (Upland Crops Division, National Yeongnam Agricultural Experiment Station, RDA.)
  • Published : 2002.09.30
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Abstract

Platycodin D was isolated from n-butanol extract of Platycodi radix(Platycodon grandiflorum and identified by the spectroscopic analysis using $^1H$ and $^{13}C$ NMR techniques. A new method of analysis of platycodin D by high performance liquid chromatography(HPLC) was established using a reversed phase system with YMC-Pack ODS-AM( 250 X 4.6 mm) column and 30% acetonitrile as a mobile phase. Evaporative light scattering detector was used as detector. The kinds of extraction solvents and methods were examined to determine the efficient extraction condition and HPLC analysis was carried out to establish the optimum drying condition for the root of Platycodon grandiflorum. The contents of Platycodin D was highest as 0.083% when platycodon roots were dried at $60^{\circ}C$ using dry oven.

길경의 부탄올 분획으로부터 지표성분을 분리, 정제하기 위해 $SiO_2$ 컬럼크마토그래피를 실시한 결과 1종(화합물 1)의 단일 물질을 순수 분리하였으며 화합물 1의 구조는 $^1H$, $^{13}C$ NMR 등 분광학적 관측 결과 platycodin D로 확인되었다. Platycodin D의 HPLC 정량법을 검토한 결과, 검출기는 증기화광산란검출기(ELSD)를, 컬럼은 역상컬럼을 사용하여 길경 MeOH 추출액으로부터 효율적인 지표성분 정량이 가능하였다. 추출조건을 재검토한 결과 환류추출보다는 초음파 추출이 더 효율적이었으며 추출용매로는 80% MeOH이 다른 용매조건보다 추출효율이 우수하였다. 건조조건의 검토에서는 platycodin D의 함량이 양건이나 양건과 건조기 혼용건조 보다 건조기 단독 건조시 더 높았으며 특히 $60^{\circ}C$ 열풍건조에서 그함량이 0.083%로서 가장 높았다. 묽은 에탄올 엑스 함량도 건조기만을 이용한 열풍건조가 양건과 건조기 열풍건조를 혼용한 방법보다 4% 정도 더 높았다.

Keywords

References

  1. Ahn JC, Hwang B, Tada H, Ishimaru K, Sasaki K, Shimomura K (1996) Polyacetylenes in hairy roots of Platycodon grandiflorum. Phytochemistry 42(1) : 69-72 https://doi.org/10.1016/0031-9422(95)00849-7
  2. Arai I, Komatsu Y, Hirai Y, Shingu K, Ida Y, Yamaura H, Yamamoto T, Kuroiwa Y, Sasaki K, Taguchi S (1997) Stimulative effects of saponin from kikyo-to, a japanese herbal medicine, on pancreatic exocrine secretion of conscious rats. Planta Medica 63 : 419-424 https://doi.org/10.1055/s-2006-957725
  3. Choi CY, Kim JY, Kim YS, Chung YC, Seo JK, Jeong HG (2001) Aqueous extract isolated from Platycodon grandiflorum elicits the release of nitric oxide and tumor necrosis factor-$\alpha$ from murine macrophages. International Immunopharmacology 1 : 1141-1151 https://doi.org/10.1016/S1567-5769(01)00047-9
  4. Chung JH, Cho SH, Shin PG, Ryu JC, Jang DS (1997) Chemical compositions of Platycodon grandiflorus (Jacquin) A. De Candolle. Journal of the Korean Society of Agricultural Chemistiy and Biotechno1ogy 40(2) : 148-154
  5. Chung JH, Cho SH, Sunwoo S, Kwon JS, Shin PG (1996)Studies on the volatile components of Platycodon grandiflorus (Jacquin) A. De Candolle. Journal of the Korean Society ofAgricultural Chemistry and Biotechnology 39(6) : 517-520
  6. Fuzzati N, Gabetta B, Jayakar K, Pace R, Peterlongo F (1999)Liquid chromatography-electrospray mass spectrometric identification of ginsenosides in Panax ginseng roots, Journal of Chromatography A 854 : 69-79 https://doi.org/10.1016/S0021-9673(99)00463-X
  7. Han LK, Xu BJ, Kimura Y, Zheng Y, Okuda H (2000) Platycodi radix affects lipid metabolism in mice with high fat diet-induced obesity. Journal of Nutrition 130(11) : 2760-2764 https://doi.org/10.1093/jn/130.11.2760
  8. Han, SB, Park SH, Lee KH, Lee CW, Lee SH, Kim HC, Kim YS, ee HS, Kim HM (2001) Polysaccharide isolated from the dix of Platycodon grandinflorum selectively activates B cells and macrophages but not T cells, International Immunopharmacology 1 : 1969-1978 https://doi.org/10.1016/S1567-5769(01)00124-2
  9. Higashi A, Arimoto K, Iwata Y, Ujita K, Kawai T, Kobayashi Y, Sakurai K, Shimada Y, Takagi A, Taniyama T, Nakajima KI, Hisata Y, Hosoda K, Yamamoto K, Yamamoto Y, Noguchi M (1997) Studies on the effect of preparation methods of platycodon root on its quality. Natural Medicines 51(1) : 56-62
  10. Ida Y, Hirai Y, Kajimoto T, Shingu K, Miura T, Kuwahara N, Taguchi S, Sasaki K, Kuroiwa Y, Yamamoto T, Arai I, Amagaya S, Komatsu Y (1998) Requirement of the glycosyl parts in platycodin D to stimulate pancreatic exocrine secretion. Bioorganic & Medicinal Chemistiy Letters 8 : 2209-2212 https://doi.org/10.1016/S0960-894X(98)00389-8
  11. Kim KS, Ezaki O, Ikemoto S, Itakura H (1995) Effects of Platycodon grandiflorum feeding on serum and liver lipid concentrations in rats with diet-induced hyperlipidemia. Journal of Nutritional Science and Vitaminology 41 (4) : 485-491 https://doi.org/10.3177/jnsv.41.485
  12. Kim TJ, Lee SI, Lee TH, Ko JS (1990) Isolation and determination of platycodin D from platycodi radix. Journal of Korean Society of Analytical Science 3(3) : 399-404
  13. Kim YG, Lee BE, Kim KJ, Lee YT, Gho KB, Chung YC (1998) Antitumor and immunomodulatory activities of the Platycodon grandiflorum cultivated for more than 20 years. Yakhak Hoeji 42 : 382-387
  14. Konishi T, Tada A, Shoji J, Tanaka O (1976) The structures of platycodin A and C, monoacetylated saponins of the roots of Platycodon grandiflorum. Chemical and Pharmaceutical Bulletin 26(2) : 668-670
  15. Lee EB (1974) Pharmacological studies on Platycodon grandiflorum. Korean Journal of Pharmacongnogy 5 : 49-51
  16. Lee GY, Hwan EI, Lim ST (1998) Effect of Platycodon grandiflorum DC Extract on the growth of cancer cell Lines. Korean Journal of Food Science Technology 30(1) : 13-21
  17. Li W, Fitzloff JF (2001) Determination of 24(R)- pseudoginsenoside Fll in North American ginseng using high performance liquid chromatography with evaporative light scattering detection, journal of Pharmaceutical and Biomedical Analysis 25 : 257-265 https://doi.org/10.1016/S0731-7085(00)00494-5
  18. Park MK, Park JH, Han SB, Shin YG, Park IH (1996) Highperformance liquid chromatographic analysis of ginseng saponins using evaporative light scattering detection. Journal of Chromatography A 736 : 77-81 https://doi.org/10.1016/0021-9673(95)01323-7
  19. Saeki T, Koide K, Nikaide T (1999) A comparative study on commercail, botanical gardens and wild samples of the roots of PIatycodon grandtflorum by HPLC analysis. Panta Medica 65 : 428-431 https://doi.org/10.1055/s-1999-14021
  20. Tada A, Kaneiwa Y, Shoji J, Shibata S (1975) Studies on the saponins of the root of Platycodon grandiflorum A. De Candolle. I. Isolation and the structure of platycodin D. Chemical and Pharmaceutical Bulletin 23(11) : 2965-2972 https://doi.org/10.1248/cpb.23.2965