• Asian Pacific Journal of Cancer Prevention
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• 제16권14호
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• pp.5589-5594
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• 2015

Cystitis and proctitis are defined as inflammation of bladder and rectum respectively. Haemorrhagic cystitis is the most severe clinical manifestation of radiation and chemical cystitis. Radiation proctitis and cystitis are major complications following radiotherapy. Prevention of radiation-induced haemorrhagic cystitis has been investigated using various oral agents with minimal benefit. Bladder irrigation remains the most frequently adopted modality followed by intra-vesical instillation of alum or formalin. In intractable cases, surgical intervention is required in the form of diversion ureterostomy or cystectomy. Proctitis is more common in even low dose ranges but is self-limiting and improves on treatment interruption. However, treatment of radiation proctitis is broadly non-invasive or invasive. Non-invasive treatment consists of non-steroid anti-inflammatory drugs (NSAIDs), anti-oxidants, sucralfate, short chain fatty acids and hyperbaric oxygen. Invasive treatment consists of ablative procedures like formalin application, endoscopic YAG laser coagulation or argon plasma coagulation and surgery as a last resort.

• Toxicological Research
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• 제20권2호
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• pp.137-142
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• 2004

We investigated the effects of plant vinegar on platelets and blood coagulation system. Plant vinegar inhibited in vitro platelet aggregation in a concentration dependent manner, when platelets were activated by thrombin and collagen. In addition, plant vinegar showed inhibitory effects on the serotonin secretion induced by thrombin in a concentration dependent manner. However, treatment with plant vinegar to platelets did not induce any cytotoxicity, as determined by the release of lactate dehydrogenase. Plant vinegar did not change the coagulation parameters such as activated partial thromboplastin time (aPTT) and prothrombin time (PT) using rat citrated plasma. In vivo study revealed that, treatment with plant vinegar prolonged the bleeding time from mouse tail. All these results suggest that plant vinegar might improve blood hemostasis mediated via anti platelet activities.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.189-189
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• 1998

Tissue factor (TF) is a cell surface receptor of coagulation factor Ⅶ and is the principal initiator of the vertebrate coagulation cascade. TF is found in high levels in some organs such as brain, lung and placenta, whereas blood monocytes, endothelial cells contain only trivial amount of TF when quiescent, and is stimulated to synthesize TF by infections or vascular lesions. TF is reported to be found in high levels in atherosclerotic plaques, cancer cells. TF activation in various cells in many infectious or immunologic diseases tells us the physiologic importance of TF. We screened many edible vegetables for TF inhibitor, by measuring the prothrombin time to detect the TF activity, and we picked Aster scaber to isolate the TF inhibitory substance. Aster scaber showed two kinds of anti thrombotic activity, one is TF inhibition and the other is elongation of plasma recalcification time. The anti thrombotic substances were found to be saponins which has echinocystic acid as aglycone.

• Journal of the Optical Society of Korea
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• 제13권3호
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• pp.321-329
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• 2009

Following their applications in cardiology, ophthalmology and dentistry among others, the advent of lasers in dermatology and urology had become the success story of the past decade. Laser-assisted treatments in dermatology and urology are mainly based on the laser-induced tissue injury/coagulation and/or ablation, depending upon the desirable clinical endpoint. In this review, we discussed the underlying mechanisms of the laser induced tissue ablation. In any medical laser application, the controlled thermal injury and coagulation, and the extent of ablation, if required, are critical. The laser thermal mechanism of injury is intricately related to the selective absorption of light and its exposure duration, similarly to the laser induced ablation. The laser ablation mechanisms were categorized into four different categories (the photo-thermally induced ablation, the photo-mechanically induced ablation, the plasma induced ablation and the photoablation) and their fundamentals are herein described. The brief history of laser treatment modality in dermatology and urology are summarized.

• 약학회지
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• 제39권1호
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• pp.55-60
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• 1995

The acetone precipitates of the hot water extract of Torilis ftuctus showed strong hemostatic activity which was not inhibited by aspirin. This activity was not through platelet activation but possibly through activating some coagulation factors in plasma. The hemostatic action of the precipitates was not active by oral adminitration and no behavioral toxicity was appeared in treated mice. However, mice treated with the acetone precipitates through tail vein showed serious tremor and then were killed probably by the thrombus produced in the body. The hemostatic activity was still remained after treatment with $\beta$-glucosidase, $\beta$-alactosidase, $\alpha$-amylase, subtilisin BPN', or trypsin but completely lost by acid hydrolysis. The active components seemed to be a complex of unidentified macromolecules to which some phenolic compounds were strongly bound.

• Archives of Pharmacal Research
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• 제16권3호
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• pp.209-212
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• 1993

The anti-coagulant activity of ilexoside D isolated from the roots of ilex pubescens Hook. et Am. was investigated in in vivo models of blood coagulation in rats. On oral administration, ilexoside D prolonged the bleeding time and the whole blood recalcified clotting time, but not the plasma recalcified clotting time. In vitor, ilexoside D did not affect the recalciffed clotting times of whole blood, platelet-rich plasma(PRP), and platelet-poor plasma(PPP), while in the presence of tissue factor the compound prologed the reduced proth-rombin times of whole blood, PRP and PPP in the dose-dependent manner. These results indicate that ilexoside D has the anit-tissue factor activity as well as the antithromobotic activity.

• 생명과학회지
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• 제23권6호
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• pp.838-846
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• 2013

플라즈마는 물질의 제4의 상태로서 이온화된 가스로 불리며 다양한 활성종 및 수산화기, 하전입자, 이온, 자유 전자, 산소등을 발생시키는 특징을 가지고 있다. 20세기 후반에 플라즈마는 산업현장에서 활발히 사용되고 있으며, 최근 저온 상압 플라즈마 장치가 개발되면서 의생명 분야에 적용되기 시작했다. 저온 상압 플라즈마는 인체조직에 열 손상을 입히지 않을 뿐만 아니라, 암 치료, 살균, 치아미백, 지혈, 상처치유 등에서 높은 효율성을 보이고 있다. 이때 발생되는 활성종은 포유세포나 병원균에 영향을 미치는 것으로 알려져 있다. 또한, 다학제간의 연구를 통해 저온 상압 플라즈마의 활용범위는 다양한 영역으로 넓어지고 있으며, 새로운 첨단 의료기술로서의 가치가 높아지고 있다. 저온 상압 플라즈마가 포유세포와 미생물에 적용된 이후, 지난 10여 년간 급속한 발전을 통해 최근 플라즈마 메디신이란 학문영역으로 성장했다. 본 논문은 저온 플라즈마가 적용되고 있는 분야에 대해 소개하고, 플라즈마 메디신에 대한 이해를 돕고자 한다.

• Safety and Health at Work
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• 제2권3호
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• pp.282-289
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• 2011

Objectives: We sought to establish a novel method to generate nano-sized carbon black particles (nano-CBPs) with an average size smaller than 100 nm for examining the inhalation exposure risks of experimental rats. We also tested the effect of nano-CBPs on the pulmonary and circulatory systems. Methods: We used chemical vapor deposition (CVD) without the addition of any additives to generate nano-CBPs with a particle size (electrical mobility diameter) of less than 100nm to examine the effects of inhalation exposure. Nano-CBPs were applied to a nose-only inhalation chamber system for studying the inhalation toxicity in rats. The effect on the lungs and circulatory system was determined according to the degree of inflammation as quantified by bronchoalveolar lavage fluid (BALF). The functional alteration of the hemostatic and vasomotor activities was measured by plasma coagulation, platelet activity, contraction and relaxation of blood vessels. Results: Nano-CBPs were generated in the range of 83.3-87.9 nm. Rats were exposed for 4 hour/day, 5 days/week for 4 weeks to $4.2{\times}10^6$, $6.2{\times}10^5$, and $1.3{\times}10^5$ particles/$cm^3$. Exposure of nano-CBPs by inhalation resulted in minimal pulmonary inflammation and did not appear to damage the lung tissue. In addition, there was no significant effect on blood functions, such as plasma coagulation and platelet aggregation, or on vasomotor function. Conclusion: We successfully generated nano-CBPs in the range of 83.3-87.9 nm at a maximum concentration of $4.2{\times}10^6$ particles/$cm^3$ in a nose-only inhalation chamber system. This reliable method can be useful to investigate the biological and toxicological effects of inhalation exposure to nano-CBPs on experimental rats.

• 멤브레인
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• 제15권3호
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• pp.233-240
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• 2005

유용하고 중요한 의료기기인 폐 보조 장치(LAD)의 혈액 접촉 표면과 인공 폐 막에 사용하기 위한 혈액 적합성 생체 재료를 개발하기 위해 폴리프로필렌 중공사 막의 표면 개질을 수행하였다. 재료의 혈액적합성은 항응고 처리된 혈액을 사용하였고 플라스마 응고 형성, 혈소판 접착 및 플라스마 응고 활성화, 그들의 표면 혈전 형성을 평가하여 결정하였다. 실험 결과는 실리콘 코팅 중공사들에 부착한 혈소판 수가 우수한 혈액 적합성을 나타내는 폴리프로필렌에 부착한 혈소판 수보다도 상당히 더 낮았음이 확인되었다. 또한, 상대적으로 플라스마 표면 처리한 폴리프로필렌 중공사 막이 혈액 적합성 평가에서 보체 활성화 억제를 보였음이 확인되었다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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• pp.462-462
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• 2012

바이오 플라즈마의 일환으로 대기압 플라즈마 제트 장치를 개발하여 혈액 응고 실험을 하였다. 대기압 플라즈마 제트 장치는 의료용 바늘, 테프론 튜브, 유리관으로 이루어져 있다. 본 실험에 사용된 플라즈마 제트 장치는 두 전극 사이에 유전체로 사용된 유리관이 설치된 유전체 장벽 방전 플라즈마의 한 형태라 할 수 있다. 플라즈마 제트에 주입된 가스는 Ar이며 전기적, 열적 충격이 없다. 출력전압은 1.2 kV, 출력전류는 1.9 mA, 구동주파수는 40 kHz이다. 출혈이 발생한 상처에 조사한 결과, 9 초만에 혈액이 응고되는 것을 확인하였다. 또한, 멀티 플라즈마 제트 장치를 고안하였다. 플라즈마 제트에서 발생되는 플라즈마 양을 증가시킴으로서 대면적으로 활용할 수 있다.