• Korean Journal of Plant Resources
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• v.21 no.1
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• pp.91-95
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• 2008

Reactive oxygen species(ROS) or free radical-mediated oxidative stress plays an important role in the pathophysiologic process of disease state. This study investigated antioxidative effects of Phellinus linteus extract on the generation of 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical, superoxide anion radical(${O_2}^-$), hydroxyl radical(${\cdot}OH$), nitric oxide(NO), and peroxynitrite($ONOO^-$) radical and free radical-mediated protein oxidation under in vitro assay systems. This results showed that Phellinus linteus extract effectively inhibited the generation of free radicals in the all assay system with dose-dependent manner and also significantly reduced the protein oxidative level. Thus, the present study indicates that Phellinus linteus extract possesses a potent antioxidant activity and plays a beneficial role against free radical-induced oxidative injury.

• The Journal of the Korea institute of electronic communication sciences
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• v.8 no.5
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• pp.745-750
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• 2013

In many recent studies, a variety of environmental control system for practical gardening facilities such as facility house and plant factory have been proposed. However, the plants have been exposed to growth disorder and disease and pest injury because the temperature and humidity have not properly controlled so far. Therefore, a lot of damage of farmers have been reported. The air circulation fan and industrial dehumidifier have been currently utilized as the countermeasures, but they do not meet the expectation. In this study, the growth phase of each plant is recognized by using cycle-by-cycle plants growth recogniztion algorithm to provide optimal environment according to the growth phases of each plant.he productivity can be raised by using cycle-by-cycle plant growth recognition monitoring system because it optimally controls the environment by cycle that is required for plant growth.

• Journal of Ginseng Research
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• v.43 no.1
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• pp.95-104
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• 2019

Background: Oxidized low-density lipoprotein (ox-LDL) causes vascular endothelial cell inflammatory response and apoptosis and plays an important role in the development and progression of atherosclerosis. Ginsenoside compound K (CK), a metabolite produced by the hydrolysis of ginsenoside Rb1, possesses strong anti-inflammatory effects. However, whether or not CK protects ox-LDL-damaged endothelial cells and the potential mechanisms have not been elucidated. Methods: In our study, cell viability was tested using a 3-(4, 5-dimethylthiazol-2yl-)-2,5-diphenyl tetrazolium bromide (MTT) assay. Expression levels of interleukin-6, monocyte chemoattractant protein-1, tumor necrosis factor-${\alpha}$, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1 were determined by enzyme-linked immunosorbent assay and Western blotting. Mitochondrial membrane potential (${\Delta}{\Psi}m$) was detected using JC-1. The cell apoptotic percentage was measured by the Annexin V/ propidium iodide (PI) assay, lactate dehydrogenase, and caspase-3 expression. Apoptosis-related proteins, nuclear factor $(NF)-{\kappa}B$, and mitogen-activated protein kinases (MAPK) signaling pathways protein expression were quantified by Western blotting. Results: Our results demonstrated that CK could ameliorate ox-LDL-induced human umbilical vein endothelial cells (HUVECs) inflammation and apoptosis, $NF-{\kappa}B$ nuclear translocation, and the phosphorylation of p38 and c-Jun N-terminal kinase (JNK). Moreover, anisomycin, an activator of p38 and JNK, significantly abolished the anti-apoptotic effects of CK. Conclusion: These results demonstrate that CK prevents ox-LDL-induced HUVECs inflammation and apoptosis through inhibiting the $NF-{\kappa}B$, p38, and JNK MAPK signaling pathways. Thus, CK is a candidate drug for atherosclerosis treatment.

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.209-217
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• 2006

This study was carried out to investigate and analyze the vascular flora and injury status in Shinduri coastal dune area $(N36^{\circ}\;49',\;E126^{\circ}\;11')$, where designated as Natural Monuments and the result are as follows. The vascular plants identified and surveyed were 125 taxa including 108 species, 14 varieties, 2 forms and 1 hybrid of 92 genera of 40 families and there are 20 taxa including 17 species, 2 varieties and 1 hybrid of 15 genera of 10 families for woody plants, while 105 toxa including 91 species, 12 varieties and 2 forms of 78 genera of 34 families for the herbaceous plants at the . study sites. Seventeen taxa including 17 species of 15 genera of 9 families were investigated for naturalized plants and ratio of naturalization was 13.6 percents at the study sites. Glehnia littorolis Fr. Schm. and Lilium callosum S. et Z. appeared as rare and endangered plants designated in Korea Forest Service. Nine taxa in degree 1, 2 taxa in degree 2, 4 taxa in degree 3, 2 taxa in degree 4 and 2 taxa in degree 5 were represented for especialized species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.6
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• pp.513-518
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• 2006

This study was conducted to investigate the relations between brown planthopper (BPH, Nilapavata lugens) resistance and specific organic acids (oxalic acid, silicic acid, and trans-aconitic acid) known as BPH sucking inhibitors on different rice varieties and/or lines. There were no specific relations between BPH resistance and the contents of oxalic and silicic acids in the rice plant tissues. However, the stronger the BPH resistance was occurred, the higher the content of trans-aconitic acid was contained in the rice plants. The relations between the injury rate of rice plant by BPH and the content of trans-aconitic acid in the rice plants were negatively correlated, which were -0.84 and -0.82 at 30 and 60 days after seeding, respectively. Therefore, the content of trans-aconitic acid in rice plant tissues might be utilized as an index for improving BPH resistance of rice varieties.

• Korean Journal of Weed Science
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• v.12 no.3
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• pp.261-270
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• 1992

Many herbicides that are applied at the soil before weed emergence inhibit plant growth soon after weed germination occurs. Plant growth has been known as an irreversible increase in size as a result of the processes of cell divison and cell enlargement. Herbicides can influence primary growth in which most new plant tissues emerges from meristmatic region by affecting either or both of these processes. Herbicides which have sites of action during interphase($G_1$, S, $G_2$) of cell cycle and cause a subsequent reduction in the observed frequency of mitotic figures can be classified as an inhibitor of mitotic entry. Those herbicides that affect the mitotic sequence(mitosis) by influencing the development of the spindle apparatus or by influencing new cell plate formation should be classified as causing disruption of the mitotic sequence. Sulfonylureas, imidazolinones, chloroacetamides and some others inhibit plant growth by inhibiting the entry of cell into mitosis. The carbamate herbicides asulam, carbetamide, chlorpropham and propham etc. reported to disrupt the mitotic sequence, especially affecting on spindle function, and the dinitroaniline herbicides trifluralin, nitralin, pendimethalin, dinitramine and oryzalin etc. reported to disrupt the mitotic sequence, particularly causing disappearence of microtubles from treated cells due to inhibition of polymerization process. An inhibition of cell enlargement can be made by membrane demage, metabolic changes within cells, or changes in processes necessary for cell yielding. Several herbicides such as diallate, triallate, alachlor, metolachlor and EPTC etc. reported to inhibit cell enlargement, while 2, 4-D has been known to disrupt cell enlargement. One potential danger inherent in the use of soil acting herbicides is that build-up of residues could occur from year to year. In practice, the sort of build-up that would be disastrous is unikely to occur for substances applied at the correct soil concentration. Crop injury caused by soil applied herbicides can be minimized by (1) following the guidance of safe use of herbicides, particularly correct dose at correct time in right crop, (2) by use of safeners which protect crops against injury without protecting any weed ; interactions between herbicides and safeners(antagonists) at target sites do occur probably from the following mechanisms (1) competition for binding site, (2) circumvention of the target site, and (3) compensation of target site, and another mechanism of safener action can be explained by enhancement of glutathione and glutathione related enzyme activity as shown in the protection of rice from pretilachlor injury by safener fenclorim, (3) development of herbicide resistant crops ; development of herbicide-resistant weed biotypes can be explained by either gene pool theory or selection theory which are two most accepted explanations, and on this basis it is likely to develop herbicide-resistant crops of commercial use. Carry-over problems do occur following repeated use of the same herbicide in an extended period of monocropping, and by errors in initial application which lead to accidental and irregular overdosing, and by climatic influence on rates of loss. These problems are usually related to the marked sensitivity of the particular crops to the specific herbicide residues, e.g. wheat/pronamide, barley/napropamid, sugarbeet/ chlorsulfuron, quinclorac/tomato. Relatively-short-residual product, succeeding culture of insensitive crop to specific herbicide, and greater reliance on postemergence herbicide treatments should be alternatives for farmer practices to prevent these problems.

• Korean Journal of Weed Science
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• v.11 no.1
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• pp.32-49
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• 1991

Residual period and carry-over effect of some herbicides were determined using a bioassay method in five winter crops (chinese cabbage, radish, spinach, onion and garlic). The effects were measured at regular time intervals after applling different rates of the herbicides. There were no great differences in residual period and carry-over injury between the soils and kinds of crops used. However, the residual period varied with the herbicides studied and the carry-over injury was rate of the herbicide application, sampling depth of soil, and kind and seeding date of the test plant. When the residual herbicides were applied, the carry-over injury could be minimized by selecting tolerant crops, delaying seeding of the crops after application of the herbicides, and regulating the cultivation depth. Herbicides which showed no residual effect by the end of the cropping period (200-240 days for winter crops) and no carry-over effect were alachlor, trifluralin, ethalfluralin and prometryn. When pendimethalin, metolachlor, linuron and methabenthiazuron were applied at the recommended rate or less, there was no carry-over injury at harvesting time. With doubling the recommended rate, however, the carry-over effect was found in sensitive crops. Napropamide applied in winter crops at rate of 150-300g a.i./10a brought about carry-over injury for such Gramineae as Italian ryegrass, direct-seeded rice and barley, whereas the injury was not found in lowland-transplanted rice, Cruciferae, Cucurbitaceae and Solanaceae. Long residual herbicide nitralin applied at the rate of 75g a.i./10a caused the carry-over injury for Italian ryegrass, direct-seeded rice, baley and lowland-transplanted rice at 275 days in winter crops. In addition, a slight injury occurred in sesame, perilla and spinach, However, there was no injury for Cruciferae, Cucurbitaceae and Solanaceae.

• Korean Journal of Weed Science
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• v.10 no.4
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• pp.248-254
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• 1990

Initial crop injury, plant growth and yield of machine-transplanted rice of seedlings different in plant age (8-, 20-, and 35-day-old seedlings) after application of herbicides were investigated in field. The herbicides employed were butachlor, dithiopyr, pyrazolate(applied only for 8-day-old seedling), dithiopyr+pyrazolate, dithiopyr+ bensulfuron methyl), and butachlor+bensulfuron methyl(applied only for 20-and 35-day-old seedlings). There was no significant difference in plant growth, yield components and yield between the different-aged seedlings of the untreated check, except for the heading date, The heading date of 8-day-old seedling was delayed for 2 and 6 days as compared with those of 20-and 35-day-old seedlings, respectively. A very slight initial crop injury was found with the tested herbicides for all the different-aged seedlings. No significant differences in plant height and tiller number occurred between different-aged seedlings and the respective untreated check. All the herbicides used gave a good weeding effect. Yield components and yields were not affected by different-aged seedlings after application of the herbicides.

• Research in Plant Disease
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• v.13 no.2
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• pp.98-103
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• 2007

Grapevines will experience various types of winter damage. Some winter damages are caused by mechanical injury, freezing temperatures or poor vine vigor. This research was conducted to find out the appropriate control methods through selection of resistant rootstocks and improvement of overwintering methods for the control of crown gall disease on 'Kyoho' grape. The crown gall symptoms were not found when three stock plants of grapevine SO4, 5BB and 3306 were inoculated with $10^4cfu/ml$ of Agrobacterium vitis strains (YK2823, YK3312, LMG259, HKA234). But when they were inoculated with higher concentration $(10^6 cfu/ml)$ of A. vitis, irrespective of stocks plants, crown galls were formed all of them and the gall size was much smaller than that of kyoho. Three stock plants were selected as resistant based on above mentioned. Covering trunks and branches with rice straw and insulating coverlet was the most effective method for prevention of crown gall disease. This treatment minimized the ambient temperature changes on grapevine trees during winter season to $9.6^{\circ}C$ and the normal plant growth was due to the absence of freezing injury.

• Journal of Plant Biology
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• v.11 no.1
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• pp.7-14
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• 1968

Buckwheat seeds produced in previous year (1965) in an experimental pot culture in which nine levels of P-32 ranging from $1.4{\times}10-4 to 3.3{\times}104$\mu$c/pot(as of 27 July 1965)$, with the same specific activity, had been applied to the corresponding pots respectively, were used this year(1996) in water and soil culture as well as in germination test to investigate the feature and extent of possible residual effects of P-32 incorporated upon germination and plant growth, and the following results were obtained: 1. Under the given experimental conditions both stimulative and inhibitory effects of radiation were observed. 2. The germination rate of the seeds was lower at the higher level of P-32 aplication ranging from $3.0{\times}103 to 3.3{\times}104$\mu$c/pot$ and higher at 0.2 $\mu$c P/pot than the control. 3. Among the seeds produced at the higher level of application about 80% was failed to germinate, owing to the radiation injury. The remaining 80% was failed to germinate, owing to the radiation injury. The remaining 80% survived the damaging effect and showed vigorous growth and increased yield. The latter group of seeds thus proved themselves to be more radioresistant than the former. 4. The survived seeds produced later more straw and root on dry weight basis. The higher the level of P-32 applied, the stronger the stimulative effect showed in vegetative growth. 5. No radiation effect on linear growth of the plants was observed in the soil culture. 6. The dry weight of straw produced showed little difference at the moderate range compared with that of control in the soil culture. At high level of application, i.e. over $\mu$c P32/pot, however, the production was increased by 12-37% of control. 7. As for the dry weight of root harvested, the P-32 treatment over 24$\mu$c P32/pot produced 82-155% more than the control, whereas little difference was observed under 2.2$\mu$c P32/pot. 8. The seed production increased in general by the P-32 treatments. Particularly at the moderate level of application the rate of increase amounted to 70% of the control. 9. Those individual plants which survived damaging effects of radiation at the germinating stage shwoed remarkable stimulative effects both in vegetative and in reproductive stage of growth.