• Exercise Science
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• v.26 no.3
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• pp.229-237
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• 2017

PURPOSE: This study was to investigate the Glycolysis mediated sarcoplasmic reticulum (SR) $Ca^{2+}$ signal regulates mitochondria $Ca^{2+}$ during skeletal muscle contraction by using glycolysis inhibitor. METHODS: To examine the effect of Glycolysis inhibitor on SR and mitochondria $Ca^{2+}$ content, we used skeletal muscle fiber from gastrocnemius muscle. 2-deoxy glucose and 3-bromo pyruvate used as glycolysis inhibitor, it applied to electrically stimulated muscle contraction experiment. Intracellular $Ca^{2+}$ content, SR, mitochondria $Ca^{2+}$ level and mitochondria membrane potential (MMP) was detected by confocal microscope. Mitochondrial energy metabolism related enzyme, citric acid synthase activity also examined for mitochondrial function during the muscle contraction. RESULTS: Treatment of 2-DG and 3BP decreased the muscle contraction induced SR $Ca^{2+}$ increase however the mitochondria $Ca^{2+}$ level was increased by treatment of inhibitors and showed and overloading as compared with the control group. Glycolysis inhibitor and thapsigargin treatment showed a significant decrease in MPP of skeletal muscle cells compared to the control group. CS activity significantly decreased after pretreatment of glycolysis inhibitor during skeletal muscle contraction. These results suggest that regulation of mitochondrial $Ca^{2+}$ levels by glycolysis is an important factor in mitochondrial energy production during skeletal muscle contraction CONCLUSIONS: These results suggest that mitochondria $Ca^{2+}$ level can be regulated by SR $Ca^{2+}$ level and glycolytic regulation of intraocular $Ca^{2+}$ signal play pivotal role in regulation of mitochondria energy metabolism during the muscle contraction.

• Ocean and Polar Research
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• v.30 no.4
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• pp.379-399
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• 2008

Spatial-temporal variations in physiochemical water qualities (temperature, salinity, DO, SPM, POC and nutrients) of surface and bottom waters were investigated along the mid-western coastal area (Taean Peninsula to Gomso Bay) of Korea. Spatial distribution patterns of temperature and salinity were mostly controlled by the physical mixing process of freshwater from Geum River and/or Gyunggi Bay with nearby coastal water. A strong tidal front is formed off Taean Peninsula during spring and summer. Seasonal variations in nutrient concentrations, lower in spring and summer and higher in fall and winter, are primarily regulated by magnitude of phytoplankton occurrence rather than freshwater loadings into the bay. Based on seasonal and spatial variability of physicochemical parameters, water quality of the study area can be divided into four water masses; Gyunggi Bay-influenced Water Mass (GBWM), Geum River-influenced Water Mass (GRWM), Yellow Sea Bottom Cold Water Mass (YSBCWM) and Cheonsu Bay Water Mass (CBWM). Water quality of the GBWM (Taean Peninsula coastal area), which has relatively low salinity and high concentrations of nutrients, is strongly controlled by the Gyunggi Bay coastal water, which is under influence of the Han River freshwater. In this water mass, the mixed layer is always developed by strong tidal mixing. As a result, a tidal front is formed along the offshore boundary of the mixed layer. Such tidal fronts probably play an important role in the distribution of phytoplankton communities, SPM and nutrients. The GRWM, with low salinity and high nutrients, especially during the flood summer season, is closely related to physiochemical properties of the Geum River. During the flood season, nutrient-enriched Geum River water mass extends up to 60 km away from the river mouth, potentially causing serious environmental problems such as eutrophication and unusual and/or noxious algal blooms. Offshore (<$30{\sim}40m$ in water depth) of the study area, YSBCWM coupled with a strong thermocline can be identified in spring-summer periods, exhibiting abundant nutrients in association with low temperature and limited biological activity. During spring and summer, a tidal front is formed in a transition zone between the coastal water mass and bottom cold water mass in the Yellow Sea, resulting in intensified upwelling and thereby supplying abundant nutrients to the GBWM and GRWM. Such cold bottom water mass and tidal front formation seems to play an important role in controlling water quality and further regulating physical ecosystem processes along mid-western Korean coastal area.

• Journal of the Korea Convergence Society
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• v.7 no.5
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• pp.269-277
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• 2016

This study was aimed to study the effects of adolescents' outdoor sports and e-sports activities on their holistic health. In order for this, adolescent holistic health has been divided into three categories: physical health, mental health, and social health; and reviewed existing research on outdoor sports and e-sports. The research has found several variables for measuring the correlation between the two types of sports. The variables cover four areas: 1) General Information, determined by gender, age, preferred sport, and length of play; 2) Physical Health, as measured by six questions in the basic PAPS (Physical Activity Promotion System) survey; 3) Mental Health, as measured by stress index, self-efficacy, subjective health, and self-esteem; and 4) Social Health, as measured by variables relating to family, friends, school, and society. A total of 36 questions were devised, with 1-4 questions in each area. Primarily it examined the mental health social health. As a result, it was found that three factors have a difference such that the significance of self satisfaction. family relationships, friendships is 0.064, 0.012, 0.088 respectably.

• Journal of Life Science
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• v.18 no.9
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• pp.1278-1283
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• 2008

The purpose of this study was to investigate the effects of 12-week aerobic exercise training on cardiac QT intervals and cardiovascular risk profiles in type 1 diabetic children. Eleven type 1 diabetic children aged 9-17 years took part in this study as subject. The participants performed aerobic exercise training three times a week for total 12 weeks. The intensity of the training was adjusted at HRR 45% until three weeks and at HRR 55% since four weeks. After a 12-week aerobic exercise training systolic (p<0.05) and diastolic (p<0.05) blood pressure of the subjects was decreased. There was significant decrease in cardiac QT intervals (p<0.05), TC level (p<0.05), and HDL-C level (p<0.05). Therefore, the results of this study suggest that long-term regular physical activity of type 1 diabetic children considered exercise intensity and frequency may effect and play a important role in the prevention of diabetic complications and cardiovascular health care.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.5
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• pp.89-97
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• 2021

The purpose of this study was to examine the awareness of early childhood teachers for early childhood creativity programs using physical computing in an effort to provide future direction for program development. The findings were as follows: First, 43.8 percent of teachers answered they were neutral about the need for these programs, while 40.5 percent replied these programs were necessary. Second, 73.8 percent thought the utilization of physical computing tools would be useful. Third, the largest group thought that the programs should be provided for five-year-olds. Regarding the frequency of programs, once or twice a week were considered most appropriate. Concerning the length of each activity, 20 to 30 minutes was identified as ideal, and study subjects preferred small group activities. In regard to the most effective theme for these programs, tools for daily life were considered best. As for educational content to be included in program planning, scientific inquiry and experiment were viewed as best, followed by the design/ production/utilization of creative works, game and play, and producing and playing musical instruments. Evaluation by a creativity test was chosen as the most desirable evaluation method.

• The Journal of Sustainable Design and Educational Environment Research
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• v.18 no.1
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• pp.49-61
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• 2019

In this study, interviews are held with the faculties of two elementary schools on narrow sites located in Seoul to examine the existing problems of the elementary school on narrow site and the need for the new facilities of the elementary school on narrow site in order to derive the implications for the planning guidelines of such school construction. As a result of the survey, it was found that the substitutes of the playground installed in the elementary school on narrow site were not suitable for physical play activities, physical education class activities, and other curriculum operations. In order to secure an adequate physical activity an space for elementary schools, faculty members were demanding that the entire floor or rooftop of the school building be spatialized for physical activities. Additionally, problems such as noise generation and the lack of special rooms and public space were occurring in the elementary schools on narrow sites and are being deteriorated by accommodating an over number of students, and therefore policies are required to ensure that the appropriate number of students are maintained in the case of the schools on narrow sites.

• Journal of Ginseng Research
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• v.47 no.6
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• pp.726-734
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• 2023

Background: Skeletal muscles play a key role in physical activity and energy metabolism. The loss of skeletal muscle mass can cause problems related to metabolism and physical activity. Studies are being conducted to prevent such diseases by increasing the mass and regeneration capacity of muscles. Ginsenoside Rg5 has been reported to exhibit a broad range of pharmacological activities. However, studies on the effects of Rg5 on muscle differentiation and growth are scarce. Methods: To investigate the effects of Rg5 on myogenesis, C2C12 myoblasts were induced to differentiate with Rg5, followed by immunoblotting, immunostaining, and qRT-PCR for myogenic markers and promyogenic signaling (p38MAPK). Immunoprecipitation confirmed that Rg5 increased the interaction between MyoD and E2A via p38MAPK. To investigate the effects of Rg5 on prevention of muscle mass loss, C2C12 myotubes were treated with dexamethasone to induce muscle atrophy. Immunoblotting, immunostaining, and qRT-PCR were performed for myogenic markers, Akt/mTOR signaling for protein synthesis, and atrophy-related genes (Atrogin-1 and MuRF1). Results: Rg5 promoted C2C12 myoblast differentiation through phosphorylation of p38MAPK and MyoD/E2A heterodimerization. Furthermore, Rg5 stimulated C2C12 myotube hypertrophy via phosphorylation of Akt/mTOR. Phosphorylation of Akt induces FoxO3a phosphorylation, which reduces the expression of Atrogin-1 and MuRF1. Conclusion: This study provides an understanding of how Rg5 promotes myogenesis and hypertrophy and prevents dexamethasone-induced muscle atrophy. The study is the first, to the best of our knowledge, to show that Rg5 promotes muscle regeneration and to suggest that Rg5 can be used for therapeutic intervention of muscle weakness and atrophy, including cancer cachexia.

• Journal of the Korean Institute of Landscape Architecture
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• v.48 no.2
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• pp.45-55
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• 2020

In terms of environmental justice, urban parks play a pivotal role in imperative amenities allowing for physical activity. The reasonable distribution of urban park services must be considered in terms of community demand and the context of the park. The purpose of this study is to analyze the inequity of qualitative park service according to the socioeconomic status(SES) in Daegu. The qualitative service was assessed for 82 neighborhood and walking-distance parks by utilizing the NGST(Neighborhood Green Space Tool). The inequity was analyzed by SES variables(ratio of basic living recipients, ratio of single-parent families, average housing sales, dilapidated dwelling ratio, and park area per capita). The features of the qualitative equity in Daegu is as follows. First, urban park planning in Daegu is in parallel with the development of residential areas rather than the local policy. The development pattern of parks stretching from center of the city to outskirts clearly dissociates the city based on socioeconomic status. The parks in the center are relatively old and poorly managed. Second, overall neighborhood parks lacked recreational facilities. The facilities are significantly influenced by the housing values around them. The lower the recreation facility score, the higher the floor gradient of the urban parks constraining physical activities. Third, the quantitative supply of parks has nothing to do with the quality of the urban parks. Green space distribution is highly dependent on natural park areas, so the park area per capita cannot be a standard for assessing qualitative equity.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2005.04a
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• pp.53-60
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• 2005

Parkinson's disease (PD) is a progressive neurodegenerative disorder affecting $1\%$ of the population above the age of 65 and is characterized by a selective loss of dopaminergic neurons in the substantia nigra pars compacta. Although the underlying cause of dopaminergic cell death or the mechanism by which these cells degenerate is still not clearly understood, oxidative stress, mitochondrial dysfunction, and protein misfolding are thought to play important roles in the dopaminergic degeneration in PD. Tetrahydrobiopterin (BH4) is synthesized exclusively in the monoaminergic, including dopaminergic, cells and serves as an endogenous and obligatory cofactor for syntheses of the potential oxidative stressors dopamine and nitric oxide. In addition to its contribution toward the syntheses of these two potentially toxic molecules, BH4 itself can directly generate oxidative stress. BH4 undergoes oxidation during the hydroxylation reaction as well as nonenzymatic autooxidation to produce hydrogen peroxide and superoxide radical. We have previously suggested BH4 as an endogenous molecule responsible for the dopaminergic neurodegeneration. BH4 exerts selective toxicity to dopamine-producing cells via generation of oxidative stress, mitochondrial dysfunction, and apoptosis. BH4 also induces morphological, biochemical, and behavioral characteristics associated with PD in vivo. BH4 as well as enzyme activity and gene expression of GTP cyclohydrolase I, the rate-limiting enzyme in BH4 synthesis pathway, are readily upregulated by cellular changes such as calcium influx and by various stimuli including stress situations. This points to the possibility that cellular availability of BH4 might be increased in aberrant conditions, leading to increased extracellular BH4 subsequent degeneration. The fact that BH4 is specifically and endogenously synthesized in dopaminergic cells, Is readily upregulated, and generates oxidative stress-related cell death provides physical relevance of this molecule as an attractive candidate with which to explain the mechanism of pathogenesis of PD.

• Journal of Korean Medical classics
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• v.24 no.2
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• pp.161-181
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• 2011

In spite of the development of scientific and medical technology, the rate of prevalence and death of the chronic disease gets higher. Therefore people come to know prevention is more important than treatment in disease. In Western, it is nearly after 1970's when it is revealed that the major factor of chronic disease comes from habitual behaviors in everyday life. But that is common idea in Oriental Medicine since thousand years. In Oriental Medicine, the main cause of disease is composed of natural factor including climate condition, living-life factor, psycho-social factor, constitutional factor, inbody-pathologic-substance factor, and other factors including habits of using drug and medical service, injury, environmental pollutions, and occupational etiologic factors. Among these, the major factors of disease in modern society are lifestyle factors like the tendency of choosing and intaking food, the level of physical activity and labor, smoking and drinking alcohol, psychological tendency, the habits of using drug and medical service and so on. This is a practical aspect of oriental medicine where the most important etiologic factor comes from human behaviors, not from external factors like germ or virus. So that etiology in Oriental Medicine where improvement in the way of life is needed to prevent disease would play an important role in modern society.