• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.259-264
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• 2006

This experiment was conducted to investigate the effects of medium composition, physical condition and growth regulators on prothallus proliferation in 3 fern species belonging to Aspleniaceae (Asplenium incisum, Camptosorus sibiricus and Phyllitis scolopendrium). In all of the 3 species, the effective medium for prothallus proliferation was Murashige and Skoog's basal medium with 1% sucrose and pH 5.8. The optimum $NH_4^+:NO_3^-$ concentration ratio of protahllus proliferation was 20:40mM (1:2 ratio) in A. incisum and P. scolopendrium and 30:30mM (1:1 ratio) in C. sibiricus. However, agar content of medium did not affect the rate of prothallus proliferation. Among the selected 6 kinds of growth regulators (NAA, IAA, 2,4-D, BAP, kinetin and lip), cytokinins generally has a promotive effect, but auxins showed an inhibitory effect on prothallus growth.

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.365-373
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• 2006

This study was conducted to develop the efficient propagation method of fern Phyllitis scolopendrium using In vitro culture. The influence of the origin of the donor explant sources (rhizome, stipe, three parts of blade) and the homogenization of explants was investigated. Rhizome and stipe explants showed the organogenic capacity among the five explant sources and plant regeneration was promoted by homogenization of culture material. Optimum condition for vigorous and excellent growth of multiple shoots was the half-strength MS medium with 1% sucrose concentration. Generally, addition of $NaH_2PO_4$ to media enhanced shoot multiplication. The highest rate of shoot proliferation was observed on the media containing $5{\mu}M$ NAA. Also, combination of activated charcoal $(0.1{\sim}0.2%)$ and growth regulators to growth medium prevented the formation of multiple bud primordia, 'nodule'-like bud clusters and improved the normal morphogenesis of sporopytes in P. scolopendrium.

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.348-354
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• 2006

Homogenized prothallus of 6 species (Cyrtomium falcatum, Cyrtomium caryoptideum var. coreanum, Dryopteris varia, Asplenium incisum, Camptosous sibiricus and Phyllitis scolopendrium) were treated with gamma radiation or by chemical mutagenesis with EMS, NMU, $NaN_3$ and colchicine to assess their sensitivities for each treatments and also with the aim of inducing mutations. Generally, decrease of proliferation ratio was dose-dependent and time-dependent. Based on proliferation ratio, optimum dose of gamma irradiation was $5{\sim}10krad$ except in D. varia with 20krad. Optimum condition of EMS treatment was considered as 50mM for 3h and for NMU as $5{\sim}10mM\;for\;1{\sim}3h$. Optimum condition of $NaN_3$ treatment was considered as $0.5{\sim}1mM\;for\;1{\sim}3h$. For colchicine, there were significant differences between species as to the proliferation ratios of prothallus for each treatment.