Browse > Article

Factors Affected on Plant Regeneration of Phyllitis scolopendrium (L.) Newm. In vitro  

Jeong Jin-A (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University)
Lee Cheol-Hee (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University)
Publication Information
Korean Journal of Plant Resources / v.19, no.2, 2006 , pp. 365-373 More about this Journal
Abstract
This study was conducted to develop the efficient propagation method of fern Phyllitis scolopendrium using In vitro culture. The influence of the origin of the donor explant sources (rhizome, stipe, three parts of blade) and the homogenization of explants was investigated. Rhizome and stipe explants showed the organogenic capacity among the five explant sources and plant regeneration was promoted by homogenization of culture material. Optimum condition for vigorous and excellent growth of multiple shoots was the half-strength MS medium with 1% sucrose concentration. Generally, addition of $NaH_2PO_4$ to media enhanced shoot multiplication. The highest rate of shoot proliferation was observed on the media containing $5{\mu}M$ NAA. Also, combination of activated charcoal $(0.1{\sim}0.2%)$ and growth regulators to growth medium prevented the formation of multiple bud primordia, 'nodule'-like bud clusters and improved the normal morphogenesis of sporopytes in P. scolopendrium.
Keywords
Fern; MS medium; Growth regulators; Activated charcoal;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Fernandez, H., A.M. Bertrand and R. Sanchez-Tames. 1997a. Gemmation in Osmunda regalis L. gametophyte cultured In vitro. Plant Cell Rep. 16: 358-362
2 Fernandez, H., A.M. Bertrand and R. Sanchez-Tames. 1999. Biological and nutritional aspects in fern multiplication. Plant Cell Tiss. Org. Cult. 56: 211-214   DOI
3 Hvoslef-Eide, A.K. 1991. The effect of temperature, daylength and irradiance on the growth of mother plants of Nephrolepis exaltata (L.) Schott and on the subsequent growth In vitro of runner tip explants. Sci. Hort. 47: 137-147   DOI   ScienceOn
4 Jin, Y.H. 1999. Masspropagation of pteridophyta native to Korea by tissue culture. M.S. Thesis, Chungbuk National Univ. pp.1-62
5 Camloha, M. and N. Gogala. 1991. Platycerium bifurcatum-adventitious bud and root formation without growth regulators In vitro. Acta Hort. (ISHS) 289: 89-90
6 Teng, W.L. 1997. Activated charcoal affects morphogenesis and enhances sporophyte regeneration during leaf cell suspension culture of Platycerium bifurcatum. Plant Cell Rep. 17: 77-83   DOI
7 Wetmore, R.H. 1953. Carbohydrate supply and leaf development in sporeling ferns. Science 118: 578
8 Eggens, J.L. and C.P.M. Wright. 1985. Nitrogen effects on monostands and polystands of annual bluegrass and creeping bentgrass. HortScience 20: 109-110
9 Padhya, M.A. and A.R. Mehta. 1982. Propagation of fern (Nephrolepis) through tissue culture. Plant Cell Rep. 1: 261-263   DOI   ScienceOn
10 Teng, W.L. and M.C. Teng. 1997. In vitro regeneration patterns of Platycerium bifurcatum leaf cell suspension culture. Plant Cell Rep. 16: 820-824   DOI
11 Thakur R.C., Y. Hosoi and K. Ishii. 1998. Rapid In vitro propagation of Matteuccia struthiopteris (L.) Todaro - An edible fern. Plant Cell Rep. 18: 203-208   DOI
12 Haissig, B.E. 1974. Metabolism during adventitious root primordium initiation and development. For. Sci. 4: 324-337
13 Higuchi, H. and W. Amaki. 1989. Effect of 6-benzylaminopurine on the organogenesis of Asplenium nidis L. through In vitro propagation. Sci. Hort. 37: 351-359   DOI   ScienceOn
14 Fernandez, H., A.M. Bertrand and R. Sanchez-Tames, 1997b. Plantlet regeneration in Asplenium nidus L. and Pteris ensiformis L. by homogenization of BAP treated rhizomes. Sci. Hort. 68: 234-247
15 Camloha, M., N. Gogala and J. Rode. 1994. Plant regeneration from leaf explants of the fern Platycerium bifurcatum In vitro. Sci. Hort. 56: 257-266   DOI   ScienceOn
16 Salome, M., S. Pais and M. Casal. 1987. Propagation of the fern Adiantum capillus-veneris through tissue culture of the circinate part of young leaves. Acta Hort. (ISHS) 212: 651-654
17 Higuchi, H., W. Amaki and S. Suzuki. 1987. In vitro propagation of Nephrolepis cordifolia Prsel. Sci. Hort. 32: 105-113   DOI   ScienceOn
18 Pak, M.K. 1961. Flora of Korean Pteridophyta. Kyohakdoso Co., Seoul
19 Fernandez, H., A.M. Bertrand and R. Sanchez-Tames. 1993. In vitro regeneration of Asplenium nidis L. from gametophytic and sporophytic tissue. Sci. Hort. 56: 71-77   DOI   ScienceOn
20 Paek, K.Y., H.C. Lee, J.K. Choi and B.H. Kwack. 1984. Masspropagation Nephrolepis exaltata by runner tips In vitro. J. Kor. Soc. Hort. Sci. 25: 313-321
21 Jones, D.L. 1987. Encyclopedia of ferns. Timer press, Portland
22 Bertrand, A.M., M.A. Albuerne, H. Fernandez, A. Gonzalez and R. Sanchez-Tames. 1999. In vitro organogenesis of Polypodium cambricum. Plant Cell Tiss. Org. Cult. 57: 65-69   DOI
23 Garcia, E. and L. Furelli. 1987. Clonal mass propagation of the fern Cyrtomium falcatum. Acta Hort. (ISHS) 212: 655-660