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Effect of Medium Composition on In vitro Prothallus Culture of 3 Fern Species in the Family Aspleniaceae  

Jeong Jin-A (Dept. of Horticultural Science, Chungbuk National Univ.)
Lee Cheol-Hee (Dept. of Horticultural Science, Chungbuk National Univ.)
Publication Information
Korean Journal of Plant Resources / v.19, no.2, 2006 , pp. 259-264 More about this Journal
Abstract
This experiment was conducted to investigate the effects of medium composition, physical condition and growth regulators on prothallus proliferation in 3 fern species belonging to Aspleniaceae (Asplenium incisum, Camptosorus sibiricus and Phyllitis scolopendrium). In all of the 3 species, the effective medium for prothallus proliferation was Murashige and Skoog's basal medium with 1% sucrose and pH 5.8. The optimum $NH_4^+:NO_3^-$ concentration ratio of protahllus proliferation was 20:40mM (1:2 ratio) in A. incisum and P. scolopendrium and 30:30mM (1:1 ratio) in C. sibiricus. However, agar content of medium did not affect the rate of prothallus proliferation. Among the selected 6 kinds of growth regulators (NAA, IAA, 2,4-D, BAP, kinetin and lip), cytokinins generally has a promotive effect, but auxins showed an inhibitory effect on prothallus growth.
Keywords
Asplenium incisum; Camptosorus sibiricus; Phyllitis scolopendrium; Media composition; Proliferation;
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