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Effects of $\gamma-irradiation$ and Chemical Mutagenesis on In vitro Culture of Fern Prothallus  

Jeong Jin-A (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University)
Lee Cheol-Hee (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University)
Publication Information
Korean Journal of Plant Resources / v.19, no.2, 2006 , pp. 348-354 More about this Journal
Abstract
Homogenized prothallus of 6 species (Cyrtomium falcatum, Cyrtomium caryoptideum var. coreanum, Dryopteris varia, Asplenium incisum, Camptosous sibiricus and Phyllitis scolopendrium) were treated with gamma radiation or by chemical mutagenesis with EMS, NMU, $NaN_3$ and colchicine to assess their sensitivities for each treatments and also with the aim of inducing mutations. Generally, decrease of proliferation ratio was dose-dependent and time-dependent. Based on proliferation ratio, optimum dose of gamma irradiation was $5{\sim}10krad$ except in D. varia with 20krad. Optimum condition of EMS treatment was considered as 50mM for 3h and for NMU as $5{\sim}10mM\;for\;1{\sim}3h$. Optimum condition of $NaN_3$ treatment was considered as $0.5{\sim}1mM\;for\;1{\sim}3h$. For colchicine, there were significant differences between species as to the proliferation ratios of prothallus for each treatment.
Keywords
$\gamma-radiation$; EMS; NMU; $NaN_3$; Colchicine;
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