Effects of $\gamma-irradiation$ and Chemical Mutagenesis on In vitro Culture of Fern Prothallus

$\gamma$-선 및 화학돌연변이원 처리가 양치식물 전엽체의 기내배양에 미치는 영향

  • Jeong Jin-A (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee Cheol-Hee (Dept. of Horticultural Science and Research Center for Bioresource and Health, Chungbuk National University)
  • 정진아 (충북대학교 원예과학과, 생물건강산업개발연구센터) ;
  • 이철희 (충북대학교 원예과학과, 생물건강산업개발연구센터)
  • Published : 2006.04.01

Abstract

Homogenized prothallus of 6 species (Cyrtomium falcatum, Cyrtomium caryoptideum var. coreanum, Dryopteris varia, Asplenium incisum, Camptosous sibiricus and Phyllitis scolopendrium) were treated with gamma radiation or by chemical mutagenesis with EMS, NMU, $NaN_3$ and colchicine to assess their sensitivities for each treatments and also with the aim of inducing mutations. Generally, decrease of proliferation ratio was dose-dependent and time-dependent. Based on proliferation ratio, optimum dose of gamma irradiation was $5{\sim}10krad$ except in D. varia with 20krad. Optimum condition of EMS treatment was considered as 50mM for 3h and for NMU as $5{\sim}10mM\;for\;1{\sim}3h$. Optimum condition of $NaN_3$ treatment was considered as $0.5{\sim}1mM\;for\;1{\sim}3h$. For colchicine, there were significant differences between species as to the proliferation ratios of prothallus for each treatment.

6 종(도깨비고비, 참쇠고비, 족제비고사리, 꼬리고사리, 거미고사리 및 변산일엽)의 양치식물에서 돌연변이원 처리에 따른 영향을 구하고 인위적인 돌연변이를 유도하기 위하여, 균질화한 전엽체에 감마선 및 EMS, NMU, $NaN_3$등의 회학돌연변이원과colchicine을 처리하였다. 전엽체 증식률은 전반적으로 처리농도 및 처리시간에 비례하여 감소되는 경향을 보였다. 전엽체의 증식률에 근거할 때, 감마선 처리의 최적 조건은 족제비고사리의 경우 20krad였으며, 그 나머지 종은 $5{\sim}10krad$ 수준이었다. EMS의 적정 처리조건은 50mM에서 3시간이었으며, NMU는 $5{\sim}10mM$에서 $1{\sim}3$시간 수준이었다. 그리고 $NaN_3$ 처리의 적정조건은 $0.5{\sim}1mM$ 에서 $1{\sim}3$시간이었으며, colchicine의 경우 각각의 처리조건에서 전엽체 증식률은 종에 따라 다소 차이를 보였다.

Keywords

References

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