• Title/Summary/Keyword: Phycocyanin

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Pigment Stability of Lavers Porhyra tenera Kjellman during Processing and Storage (김의 가공 저장중에 있어서의 색소의 안정도)

  • LEE Kang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.2 no.2
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    • pp.105-133
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    • 1969
  • The stability of three major pigments in lavers, namely chlorophylls, carotenoids, and phy-cobilins, during processing and storage was studied. The results of the pigment retention in fresh lavers during storage at low temperatures, of heat lability during heat treatment, and of the pigment loss during storage are summarized as follows: 1. The contents of chlorophyll a, xanthophyll (lutein+zeaxanthin), and carotene ($\alpha-+\beta-carotene$) averaged 1,525, 627, and $409{\mu}g/g$ of dry samples respectively The comparative composition of four carotenoids, lutein, $\beta-carotene$, zeaxanthin, and $\alpha-carotene$, was 35.9, 33.7, 12.2, and $5.8\%$ respectively. 2. In storage of fresh lavers at room ternperature ($20-25^{\circ}C$), 2 to $5^{\circ}C$, and $-15^{\circ}C$, the pigments generally were retained more at 2 to $5^{\circ}C$ than others while carotene only showed more retention at $-15^{\circ}C$ than at 2 to $5^{\circ}C$. Phycocyanin tended to decrease more rapidly than phyco-erythrin at low temperatures. 3. In regard to heat treatment of both dried and wet materials at 40, 60, 80, $100^{\circ}C$ respectively, the pigments were more stable at lower temperatures than at higher temperatures in both cases of materials. Carotenoids were retained more in wet materials than in the sun dried. Xanthophylls showed higher heat stability than carotenes. Phycoerythrin was considered more heat labile than phycocyanin, particularly at higher temperatures. 4. In storage of heat treated lavers for 50 days at room temperature, the retention of pigments appeared differently in accordance with the temperatures of treatment The materials treated at $60^{\circ}C$ showed better relention of pigments than those treated at the other temperatures. Degradation rate of xanthopllylls, carotenes, and phycobilins was decreased in the materials treated at higher temperatures, showing a tendency of great pigment retention even for a long period storage. Chlorophyll il was retained more in sun dried materials whereas xanthophyll and carotene were retained more in the wet. 5. In order to maintain a higher pigment stability during storage, the materials must be treated at $60^{\circ}C$ in a dryer with rapid ventilation until the moisture level reaches about $6.0\%$. In case of treatment at a higher temperature than $60^{\circ}C$, they must be treated for a short period of time to minimize pigment degradation by heat. 6. The lavers treated with the solutions of pH around 7.8 showed a higher retention of chlorophyll a. When treated with the solutions of algal extracts such as agar and alginic acid, uniform layer of laver sheets and better surface gloss of dried layers were obtained.

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Effect of dietary pigment source on shell color of abalone, Haliotis discus hannai

  • Lim, Tae-Jun;Lee, Sang-Min
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.71-71
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    • 2003
  • This study was conducted to investigate effects of dietary pigment sources on shell color of juvenile abalone, Haliotis discus hannai. Three replicate groups of the abalone, average weight 173 mg, were fed the diets containing various pigment sources such as Porphyra powder, Spirulina, yeast astaxanthin and paprika extract for 16 weeks. Survival and weight gain were not affected by dietary pigment sources (P>0.05). Shell color of abalone fed diets containing Porphyra powder and Spirulina was approached to yellow-red and orange which are similar to shell color of wild abalone. However, shell color of abalone fed the diets containing yeast astaxanthin and paprika extract were similar to that of control diet showing bright green. Porphyra powder and Spirulina contain not only fat-soluble pigment such as chlorophyll and carotenoids but also water-soluble pigment such as phycoerythrin and phycocyanin. These results would be useful information to change shell color of abalone in aquaculture.

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Characteristic of Arthrospira platensis Enhanced Antioxidant Activity (항산화능이 증대된 Arthrospira platensis 균주 개량 및 특성 분석)

  • Choi, Soo-Jeong;Lee, Jae-Hwa
    • KSBB Journal
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    • v.30 no.3
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    • pp.119-124
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    • 2015
  • Arthrospira platensis (A. platensis) is a microscopic and filamentous cyanobacterium that derives its name from the spiral or helical nature of its filaments. In this study, we induced mutants of A. platensis through NMU treatment and selected two strains by level of ipid contents. We named mutant '1-9', '2-5', and they were cultivated in the same way with the wild type. During 12 days cultivation, cell growth, dry cell weight, pigment content, and lipid content were measured for characteristics of mutants. As a result, pigment and lipid content of mutants were increased about 3.6, 1.8 times compared with wild type, respectively. It was shown that total flavonoid and polyphenol contents of mutants were increased about 1.5 times compared with wild type. And radical scavenging effect of mutants were increased about 10% compared with wild type.

Amino Acid Composition and Antioxidative Activities of Mutant Pyropia yezoensis (방사선 돌연변이 방사무늬김(Pyropia yezoensis)의 성분 분석과 항산화 활성)

  • Choi, Jong-il
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.53 no.4
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    • pp.524-529
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    • 2020
  • Recently, several mutant laver Pyropia yezoensis strains were developed. In this study, control laver Pyropia yezoensis and its mutant Py500G were compared for their amino acid contents and antioxidative activities. There was no significant difference of total amino acid contents between wild-type and mutant Py500G, but mutant had higher free amino acid contents than wild-type. Also, total phenolic content was higher in mutant Py500G. Phycoerythrin and phycocyanin contents were higher in wild-type. But, antixoidative activities were shown to be higher in mutant Py500G. These results will be useful for the development of new cultivar of Pyropia.

Assessment of Salinity-Induced Antioxidative Defense System of Diazotrophic Cyanobacterium Nostoc muscorum

  • Srivastava, Ashish Kumar
    • Journal of Microbiology and Biotechnology
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    • v.20 no.11
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    • pp.1506-1512
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    • 2010
  • The present study examined the salinity-induced oxidative damage and differential response of enzymatic and nonenzymatic antioxidants of Nostoc muscorum. As compared with carotenoid content that showed induction, the chlorophyll and phycocyanin contents were inhibited after salt stress. Acceleration of lipid peroxidation and peroxide production suggested the onset of oxidative damage. The activities of all studied enzymatic antioxidants were significantly increased by salt stress, with maximum induction occuring with superoxide dismutase (154.8% at 200 mM NaCl treatment). Interestingly, under severe stress condition (250 mM NaCl), ascorbate peroxidase seemed to be more crucial than catalase for peroxide scavenging. Among the studied nonenzymatic antioxidants, ${\alpha}$-tocopherol was induced maximally (56.0%); however, ascorbate and reduced glutathione were increased by only 8.9% after 250 mM NaCl treatment as compared with control cells. Therefore, salinity was found to induce the antioxidative defense system of N. muscorum.

Manufacturing and Quality Characteristics of Low-salt Seasoned Gim using Spirulina (스피룰리나를 이용한 저염 조미김 제조 및 품질 특성)

  • Kyumin Kim;Jeongseop Lee;Ju-Sang Lee;Jin-Won Seok;Seon-Heui Cha
    • Journal of Marine Bioscience and Biotechnology
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    • v.15 no.2
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    • pp.90-95
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    • 2023
  • This study aimed to develop low-salt seasoned Gim (김) with new functionality to meet the diversification of the export market and consumer demand for Gim, which is one of the four major seafood products in Chungcheongnam-do province. Spirulina, a microalgae containing essential amino acids, vitamins, and minerals, is known to have antioxidant components such as phycocyanin. Therefore, to determine the suitability of spirulina as a seasoning material, we conducted seaweed. It can be used as a substitute for seasoning salt in the development of low-salt seasoned laver with enhanced functionality.

A Study on Model Improvement using Inherent Optical Properties for Remote Sensing of Cyanobacterial Bloom on Rivers in Korea (국내 수계의 남조류 원격모니터링을 위한 고유분광특성모델 개선 연구)

  • Ha, Rim;Nam, Gibeom;Park, Sanghyun;Shin, Hyunjoo;Lee, Hyuk;Kang, Taegu;Lee, Jaekwan
    • Journal of Korean Society on Water Environment
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    • v.35 no.6
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    • pp.589-597
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    • 2019
  • The purpose of this study was improve accuracy the IOPs inversion model(IOPs-IM) developed in 2016 for phycocyanin(PC) concentration estimation in the Nakdong River. Additionally, two optimum models were developed and evaluated with 2017 measurement field spectral data for the Geum River and the Yeongsan River. The used measurement data for IOPs-IM analyzation was randomly classified as training and verification materials at the ratio of 2:1 in all data sets. Using the training data set from 2015-2017, accuracy results of the IOPs-IM generally improved for the Nakdong River. The RMSE(Root Mean Square Error) decreased by 14 % compared to 2016. For the GeumRiver, the results of the IOPs-IM were suitable, except for some point results in 2016. Results of the IOPs-IM in the Yeongsan River followed the overall 1:1 line and MAE(Mean Absolute Error) was lower than other rivers. But the RMSE and MAE values were higher. As a result of applying the validation data to the IOPs-IM, the accuracy of the Nakdong River was reduced to RMSE 17.7 % and MRE 16.4 %, respectively compared with 2016. However, the MRE(Mean Relative Error) was estimated to be higher by 400 % in the Geum River, and the RMSE was more than 100 mg/㎥ of the Yeongsan River. Therefore, it is necessary to get the continuously data with various sections of each river for obtain objective and reliable results and the models should be improved.

Abundance of Autotrophic Picoplankton and Their Contribution to Phytoplankton Biomass in Korean Lakes (국내 호소에서 autotrophic picoplankton의 밀도 및 식물플랑크톤 생물량에 대한 기여도)

  • Kim, Bom-Chul;Jun, Man-Sig;Heo, Woo-Myung;Kim, Ho-Sub;Choi, Yon-Kyu
    • Korean Journal of Ecology and Environment
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    • v.34 no.3 s.95
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    • pp.141-152
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    • 2001
  • Abundance of autotrophic picoplankton (APP) and their contribution to phytoplankton biomass were assessed in seven brackish lagoons and five freshwater reservoirs in the summer season. Phycocyanin-rich picocyanobacteria dominated APP in lagoons, while phycoerythrin-rich picocyanobacteria dominated APP in freshwater reservoirs. The cell density of APP ranged from $3.6{\times}10^3$ to $5.0{\times}10^6\;cells/ml$ (median $2.5{\times}10^5$) in brackish lagoons and from $3.8{\times}10^4$ to $3.6{\times}10^5\;cells/ml$ (mdian $1.3{\times}10^5$) in reservoirs. Carbon biomass ranged from 1.0 to $1,385.0\;{\mu}gC/L$ in lagoons and from 15.3 to $128.2\;{\mu}gC/L$ in reservoirs. APP cell density in Lake Kyungpo was over $10^6\;cells/ml$in all three surveys, which is one of the highest values recorded in all over the world. During the thermal stratification in Lake Soyang, the maximum abundance of APP and their maximum contribution to phytoplankton biomass were observed near the thermocline. This study showed that APP sometimes can contribute significantly to phytoplankton biomass both in lagoons and reservoirs with the range from 0.1 to 85.0%. APP which have been overlooked in the past studies appears to be important primary producers in Korean lake ecosystem.

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Calcium-Boron Interaction in Exopolysaccharide Production by the Cyanobacterium, Nostoc spongiaeforme

  • Singh;Netu;Asthana, R.K.;Singh, S.P.
    • Journal of Microbiology and Biotechnology
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    • v.10 no.3
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    • pp.381-385
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    • 2000
  • The effect and interaction of Ca and B on exopolysaccharide (EPS) synthesis in the diazotrophically growing cyanobacterium. Nostoc spongiaeforme, was investigated. The absence of B inhibited EPS synthesis 1.56-fold ($16\mu\textrm{g}$ glucose equivalent/mg dry weight, 16 d) over the control cells ($25\mu\textrm{g}$ glucose equivalent) grown in medium containing 0.5 mM Ca and $8{\mu}{\textrm}{m}$ B. When B concentration was raised to $40{\mu}{\textrm}{m}$, EPS production was stimulated 1.8-fold. Reduction of Ca concentraion to one-half (0.25 mM) resulted in increased B demand (16$\muM$) by the cells for EPS production at par with the normal sets. However, without Ca, EPS production also increased as B increased. Addition of B to a Ca-free medium stimulated cyanobacterial diazotrophic growth as well as synthesis of Chl a and phycocyanin (0-8 d). The data suggest B-dependent diazotrophic growth during Ca-deficiency and point to and important interactive role of Ca and B in regulation of cyanobacterial EPS synthesis.

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Phycobilisome composition in Chondrus crispus (Gigartinales, Rhodophyta) from a wild type strain and its vegetatively derived green mutant

  • Cornish, M. Lynn;O' Leary, Stephen J.B.;Garbary, David J.
    • ALGAE
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    • v.28 no.1
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    • pp.121-129
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    • 2013
  • Intact phycobilisomes from a wild-type red Chondrus crispus and its vegetatively derived green mutant were isolated by centrifugation through a discontinuous sucrose density gradient. Pigment composition was subsequently characterized by spectrophotometry. Vegetative thalli of the two strains grown together for six months in the laboratory resulted in different pigment profiles. Two pigmented phycobilisome bands appeared in the sucrose gradient of the wild-type alga, a purple coloured one, and a pink one, whereas only a single blue band appeared in the gradient of the green mutant. Spectrophotometric and fluorescence analyses identified the phycobiliprotein composition of the purple band as the typical phycoerythrin-phycocyanin-allophycocyanin complement in the wild-type, but there was no detectable phycoerythrin present in the blue band of the green mutant. Sodium dodecyl sulphate, preparative polyacrylamide gel electrophoresis analysis confirmed the presence of allophycocyanin subunits in all extracts, but firm evidence of an R-phycoerythrin linker polypeptide in the blue band was missing. These results highlight the ability of C. crispus to adapt to a phycoerythrin deficiency by adjusting light harvesting pigment ratios.