DOI QR코드

DOI QR Code

Characteristic of Arthrospira platensis Enhanced Antioxidant Activity

항산화능이 증대된 Arthrospira platensis 균주 개량 및 특성 분석

  • Choi, Soo-Jeong (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University) ;
  • Lee, Jae-Hwa (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University)
  • 최수정 (신라대학교 의생명과학대학 제약공학과) ;
  • 이재화 (신라대학교 의생명과학대학 제약공학과)
  • Received : 2014.06.13
  • Accepted : 2015.06.19
  • Published : 2015.06.27

Abstract

Arthrospira platensis (A. platensis) is a microscopic and filamentous cyanobacterium that derives its name from the spiral or helical nature of its filaments. In this study, we induced mutants of A. platensis through NMU treatment and selected two strains by level of ipid contents. We named mutant '1-9', '2-5', and they were cultivated in the same way with the wild type. During 12 days cultivation, cell growth, dry cell weight, pigment content, and lipid content were measured for characteristics of mutants. As a result, pigment and lipid content of mutants were increased about 3.6, 1.8 times compared with wild type, respectively. It was shown that total flavonoid and polyphenol contents of mutants were increased about 1.5 times compared with wild type. And radical scavenging effect of mutants were increased about 10% compared with wild type.

Keywords

References

  1. Widjaja, A., C. C. Chien, and Y. H. Ju (2009) Study of increasing lipid production from fresh water microalgae Chlorella vulgaris. J. Taiwan Inst. Chem. Eng. 40: 13-20. https://doi.org/10.1016/j.jtice.2008.07.007
  2. Choi, S. P. and S. J. Sim (2012) Microalgal bioconversion to organic resources from $CO_2$. KIC News 15: 11-24.
  3. Belay, A. (2005) The potential application of Spirulina (Arthrospira) as a nutritional and therapeutic supplement in health management. JANA 5: 27-48.
  4. Joo, D. S. and S. Y. Cho (1999) The utility of microalgae and the biological activity materials in seawater microalgae. J. East Coastal Research 9: 89-108.
  5. Datla, Praneel. "The wonder molecule called phycocyanin." Chennai-India: Parry Nutraceuticals. [Disponible en linea: http://www.valensa.com/images3/Phycocyanin_The%20Wonder%20Molecule.pdf, en noviembre 2013] (2011).
  6. Manpreet, K. S., V. Kim, and S. N. Sankar (2012) Chemoprevention of DMH-induced rat colon carcinoma initiation by combination administration of piroxicam and c-phycocyanin. Molecular and Cellular Biochemistry 361: 217-218. https://doi.org/10.1007/s11010-011-1106-9
  7. Karkos, P. D., Leong, S. C., Karkos, C. D., Sivaji, N., and Assimakopouos, D. A. (2011) Spirulina in clinical practice: Envidencebaced human applications. Evid based Complement Altemat Med. 2011: 530153.
  8. Choi, S. J., Y. H. Kim, A. Kim, and J. H. Lee (2013) Arthrospira platensis mutants containing high lipid content by electron beam irradiation and analysis of its fatty acid composition. Appl. Chem. Eng. 24: 628-632. https://doi.org/10.14478/ace.2013.1085
  9. Sung, K. D., J. H. Ann, J. Y. Lee, S. J. Oh, and H. Y. Lee (1995) Kinetics of Cultivating Photosynthetic Microalga, Spirulina platensis in an Outdoor Photobioreactor. Kor. J. Biotechnol. Bioeng. 10: 401-405.
  10. Yoo, C., C. J. Kim, G. G. Choi, C. Y. Ahn, J. S. Choi, and H. M. Oh (2009) A Mutant Arthrospira platensis M20CJK3 Showing Enhanced Growth Rate and Floatation Activity. Microbiol. Soc. Kor. 45: 268-274.
  11. Cordero, B. F., I. Obraztsova, I. Couso, R. Leon, M. A. Vargas, and H. Rodriguez (2011) Enhancement of Lutein Production in Chlorella sorokiniana (Chorophyta) by Improvement of Culture Conditions and Random Mutagenesis. Mar. Drugs 9: 1607-1624. https://doi.org/10.3390/md9091607
  12. Kim, J. Y., H. Joo, and J. H. Lee (2011) Carbon Dioxide Fixation and Light Source Effects of Spirulina platensis NIES 39 for LED Photobioreactor Design. J. Kor. Ind. Eng. Chem. 22: 301-307.
  13. Kim, J. H., H. J. Park, Y. H. Kim, H. Joo, S. H. Lee, and J. H. Lee (2013) UV-induced mutagenesis of Nannochloropsis oculata for the increase of lipid accumulation and its characterization. Appl. Chem. Eng. 24: 155-160.
  14. Chen, W., M. Sommerfeld, and Q. Hu (2011) Microwave Assisted Nile Red Method for In vivo Quantification of Neutral Lipids in Microalgae. Biores. Technol. 102: 135-141. https://doi.org/10.1016/j.biortech.2010.06.076
  15. Bertozzini, E., L. Galluzzi, A. Penna, and M. magnani (2011) Application of the Standard Addition Method for the Absolute Quanitification of Neutral Lipid in microalgae using nile red. J. Microbiol. Methods 87: 17. https://doi.org/10.1016/j.mimet.2011.06.018
  16. Choi, S. J., Y. H. Kim, A. Kim, and J. H. Lee (2013) Isolation of an Arthrospira platensis Mutant Containing High Lipid Content by Electron Beam Irradiation and Analysis of its Fatty Acid Composition. Appl. Chem. Eng. 24.
  17. NFRI (1990) Manuals of Quality Chracteristic Analysis for Food Quality Evaluation (2). National Food Research Institute. Skuba, Japan, 61.
  18. Folin, O. and W. Denis (1912) On Phosphotungastic-phosphomolybdic Compounds as Color Reagents. J. Biol. Chem. 12: 239-249.
  19. Zhu, N., M. Wang, G. J. Wei, J. J. Lin, C. S. Yang, and C. T. Ho (2001) Identification of Reaction Products of (-)-Epigallocatechin, (-)-Epigallocatechin Gallate and Pyrogallol with 2,2-diphenyl-1-picryhydrazyl radical. Food Chem. 73: 2345-2349.
  20. Oh, S. H., J. G. Han, N. Y. Kim, J. S. Cho, T. B. Yim, S. Y. Lee, and H. Y. Lee (2009) Cell Growth and Lipid Production from Fedbatch Cultivation of Chlorella minutissima according to Culture Conditions. J. KSBB 24: 377-382.
  21. Boussiba, S., B. Wang, P. P. Yuan, A. Zarka, and F. Chen (1999) Changes in pigments profile in the green alga Haematococcus pluvialis exposed to environmental stresses. Biotechnol. Lett. 21: 601-604. https://doi.org/10.1023/A:1005507514694
  22. Sharma, R. (2001) Impact of solar UV-B on tropical ecosystems and agriculture. Case study: Effect of UV-B on rice. Proc. SEAWPIPT98 & SAEWPIT2000, 1: 92-101.
  23. Dube, S. L. and W. Vidaver (1992) Photosynthetic competence of plantlets grown in vitro. An automated system for measurement of photosynthesis in vitro. Physiol. Plant. 84: 409-416. https://doi.org/10.1111/j.1399-3054.1992.tb04684.x
  24. Huang, S. J. and Mau, J. L. (2006) Antioxidant properties of methanolic extracts from Agaricus blazei with various doses of $\gamma$-irradiation. Food Sci. Technol. 39: 707-716.
  25. Singleton, V. L. and J. A. Rossi (1965) Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents Am. J. Enol. Vit.16: 144-153.
  26. Merzlyak, M. N., O. B. Chivkunova, O. A. Gorelova, I. V. Reshetnikova, and A. E. Solovchenko (2007) Effect of nitrogen starvation on optical properties, pigments and arachidonic acid content of the unicellular green alga parietochloris incisa (Trevouxiophyceae, Chlorophyta). J. Phycol. 43: 833-843. https://doi.org/10.1111/j.1529-8817.2007.00375.x
  27. Liu, Z. Y., G. C. Wang, and B. C. Zhou (2008) Effect of iron on growth and lipid accumulation in Chlorella vulgaris. Bioresour. Technol. 99: 4717-4722. https://doi.org/10.1016/j.biortech.2007.09.073
  28. Kim, Y. H., S. J. Choi, H. J. Park, and J. H. Lee (2014) Electron beam-induced mutants of microalgae Arthrospira platensis increased antioxidant activity. J. JIEC 20: 1834-1840.
  29. Abd El-Daky, H. H., M. M. Hussein, and G. S. El-Baroty (2008) Algal extracts improve antioxidant defense abilities and salt tolerance of wheat plant irrigated with sea water. Afr. J. Biochem. Res. 2: 151-164.