• Journal of Nutrition and Health
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• 제43권2호
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• pp.161-170
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• 2010

We surveyed 837 students attending elementary schoolchildren in Korea for health functional foods (HFF) consumption and significant variables for their HFF consumption including demographic characteristics, parental health concern on offsprings, food eating frequency of subjects and beliefs on potential efficacy of HFF by subjects. The consumption prevalence of HFF was 45.9%, and among all types of HFF, nutritional supplements were taken most frequently, followed by apricot extract-fermented products > lactic acid bacteria containing products > EPA/DHA containing products > red ginseng products. HFF consumption was higher in subjects had parents of high education level (p < .05) and those from families with a high socioeconomic status (p < .01) in comparison to each corresponding group. Health concern on offsprings by parents (p < .001) and consumption ratio of HFF by family (p < .001) was higher in consumers than in nonconsumers of HFF. Total score of dietary assessment was higher in consumers than in nonconsumers of HFF (p < .01), and consumers had a more positive view concerning the potential efficacy of HFF than did nonconsumers (p < .001). Most consumers of HFF took HFF when they were healthy (42.5%), and they did not feel special effects through HFF consumption (47.7%). Most consumers of HFF got the information on HFF from family and relatives (24.8%), and most of them purchased HFF at pharmacy or oriental medicine clinics (53.9%). HFF consumers preferred multi-vitamins and Ca-supplements most among the vitamin mineral supplements belonged to HFF. Given the widespread consumption of HFF by elementary schoolchildren, the reasonable consumption of these products for the contribution to their overall health and well-being should be emphasized through nutrition education for them and their family.

• Bulletin of the Korean Chemical Society
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• 제29권5호
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• pp.1043-1047
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• 2008

Simultaneous determination of amitraz, bromopropylate, coumaphos, cymiazole and 2,4-dimethylaniline in 200 honey samples purchased in Korea was performed by reversed-phase high-performance liquid chromatography with multiple UV detection. 2% Acetone in hexane was used for a liquid-liquid extraction and 20-40% water in acetonitrile solutions were used as mobile phases. The LOD for the analytes varied between 0.4 and 1.5 $\mu$g/L and the recoveries were yielded between 64 and 94%. Relative standard deviation of the repeatability of the method is less than 15%. Amitraz was not present in amount above 10 $\mu$ g/L and one for coumaphos and cymiazole and two for bromopropylate, and three for 2,4-dimethylanilne were detected in amount above 10 $\mu$ g/ L. Levels of the acaricide residues found were less than 50 $\mu$ g/L.

• Journal of Microbiology and Biotechnology
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• 제16권9호
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• pp.1384-1391
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• 2006

The signal transduction system is one of the most important devices involved in maintaining life, and many protein kinases are included in the cellular signal transduction system. Finding a protein kinase inhibitor is very valuable, as it can be used to study cell biology and applied to pharmaceuticals. For the efficient and rapid screening of protein kinase inhibitors, two assay systems were combined; the nonradioactive protein kinase assay system that uses an FITC-labeled IRS-2 peptide and the cell-based paper disc assay system that uses Streptomyces griseus as the indicator strain. Among 330 kinds of herb extracts tested, the extract of Dalbergia odorifera exhibited the strongest inhibitory activity in the two assay systems and was selected for further isolation. Based on solvent extraction and many steps of chromatography, seven compounds were finally separated to homogeneity and their structures determined by $^{1}H$ and $^{13}C$ NMR spectroscopies. Four were to be flavonoids and identified as butin ($C_{15}H_{12}O_5$, Mw=272.07), 3'-hydroxymelanetin ($C_{16}H_{12}O_6$, Mw=300.06), liquiritigenin ($C_{15}H_{12}O_4$, Mw=256.07), and 2'-hydroxyformononetin ($C_{16}H_{12}O_{5}$, Mw=284.07). 3'-Hydroxymelanetin inhibited the phosphorylation of the GSK3 protein by Akt to 37% at a concentration of $10{\mu}g/ml$ and showed the strongest cytotoxicity ($ED_{50}<50{\mu}g/ml$) against the human cancer cell line HCT116. Under the same conditions, liquiritigenin also inhibited the phosphorylation of GSK3 by Akt to 26%, and its cytotoxicity against the HCT116 cell line was lower than $100{\mu}g/ml$.

• 디지털융복합연구
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• 제19권3호
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• pp.195-202
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• 2021

본 연구는 치매교육으로 제공한 국내·외 가상현실 프로그램의 특성, 효과, 적용 전략 등을 확인하는 체계적고찰 연구이다. 연구방법은 2020년 7월까지 국내·외에서 발표된 문헌을 대상으로 국외는 CINAHL, Cochrane, EMBASE, Pubmed 전자 데이터베이스를 사용하였으며, 국내는 RISS, KISS, DBpia에 대한 검색을 시행하였다. 선정기준과 배제기준을 근거로 최종 6편의 논문이 분석에 사용되었다. 분석결과 선정된 문헌의 연구대상자는 비전문 간병인 2편(33.3%), 일반인 1편(16.7%), 간호학생과 의학 및 약학대 학생을 포함한 학생들 3편(50.0%)이었다. 가상현실 프로그램은 총 5개(공감능력, 치매 지식과 편안감을 포함한 치매태도, 자신의 능력에 대한 신뢰, 양자관계척도)영역에 효과가 있었으며, 이 중 공감능력에 대한 평가가 가장 많았으며 공감능력을 측정한 연구의 75%에서 가상현실 프로그램이 유의한 효과를 나타내었다. 본 연구 결과를 통해 공감을 기반으로 한 인간중심적 간호에 가상현실 기술을 활용할 수 있음을 확인하였다.

• Parasites, Hosts and Diseases
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• 제54권2호
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• pp.155-161
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• 2016

Toxoplasma gondii is an important opportunistic pathogen that causes toxoplasmosis, which has very few therapeutic treatment options. The most effective therapy is a combination of pyrimethamine and sulfadiazine; however, their utility is limited because of drug toxicity and serious side effects. For these reasons, new drugs with lower toxicity are urgently needed. In this study, the compound, (Z)-1-[(5-nitrofuran-2-yl)methyleneamino]-imidazolidine-2,4-dione (nitrofurantoin), showed anti-T. gondii effects in vitro and in vivo. In HeLa cells, the selectivity of nitrofurantoin was 2.3, which was greater than that of pyrimethamine (0.9). In T. gondii-infected female ICR mice, the inhibition rate of T. gondii growth in the peritoneal cavity was 44.7% compared to the negative control group after 4-day treatment with 100 mg/kg of nitrofurantoin. In addition, hematology indicators showed that T. gondii infection-induced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, biochemical parameters involved in liver injury, were reduced by nitrofurantoin significantly. Moreover, nitrofurantoin exerted significant effects on the index of antioxidant status, i.e., malondialdehyde (MDA) and glutathione (GSH). The nitrofurantoin-treated group inhibited the T. gondii-induced MDA levels while alleviating the decrease in GSH levels. Thus, nitrofurantoin is a potential anti-T. gondii candidate for clinical application.

• 대한본초학회지
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• 제31권2호
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• pp.47-62
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• 2016

Objectives : This study was aimed to analyze the correlation between the Herbology and contemporary research results, KCD and terms of Alismatis Rhizoma. The study will present information that can be used to find the direction of further researches and be applied to the education of Herbology.Methods : Papers were searched in Riss4U and PubMed, and categorized. Medicine or pharmacy articles about Alismatis Rhizoma were matched with the Herbology treatment and KCD. KCD-codes and terms were arranged by the Herbology treatment. The Degree of Herbology research (HDR) was calculated by numbers of papers, study method, citation rates.Results : There were 392 articles about Alismatis Rhizoma. Among these there were 141 medicine and pharmacy articles about Alismatis Rhizoma, and 51 articles were matched to the Herbology treatment. Studies about Phlegm-Fluid Retention and Dizziness, Edema and Distention and Fullness was more active than others. Analysis of other articles showed that studies about anti-cancer, anti-biotic effect, anti-diabetes mellitus, anti-oxidant effect, anti-inflammation, hepatoprotective effect were active, too. In HDR, Phlegm-Fluid Retention and Dizziness was scored by 181, Edema- Distention and Fullness by 173, Hyperlipidemia by 83, Dysuria 64, Heat strangury pain by 18, Diarrhea and decreased urine volum by 0.Conclusions : 141 articles about Alismatis Rhizoma were analyzed and 51 articles were matched to the Herbology treatment. Studies about Phlegm-Fluid Retention and Dizziness, Edema and Distention and Fullness were more active than others. And studies about anti-cancer, anti-biotic effect, anti-oxidant effect, anti-inflammation, Osteoporosis should be more studied and be more discussed among Herbology researchers.

• 디지털융복합연구
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• 제14권5호
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• pp.427-432
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• 2016

본 연구의 목적은 수중익사 사망 환자의 임상 치료 및 예방을 알아보고자 하였다. 수중 익사 사고는 소방 방재청에서 사용한 구급 활동 일일 보고서 데이터를 사용하여 확인 하였다. 소방 방재청 통계에서 익사 사고의 특성을 확인했다. 익사 사고의 나이, 날짜, 원인, 위치, 시간 및 사례 조사 하였다. 또한, 검색은 자연적인 원인과 사례를 찾고 NEMA 웹 사이트의 수집했다. 익사 사고의 나이는 20~29년 (25.4 %)이었다. 8월 익사의 경우는 17.6 %였다. 7, 8월 즉 여름에 익사 사고의 약 30%가 발생했다. 물에 빠진 경우에 수영과 관련된 사고가 45.0 %이였다. 수영과 우발적 사고의 절반은 물에서 가족이나 친구를 구출하려고 시도하는 사람의 익사 사고를 포함되었다. 가장 일반적인 위치는 호수나 저수지에서 40.2%이었다. 호수와 해변에 있는 사람들에 대한 인명 구조, 장비 또는 구명조끼가 없었다. 익사 사고는 14시-18시 사이에 가장 많이 발생했다. 익사는 한국의 상해와 관련된 사고의 주요 원인이다. 대한민국에서 익사의 특성을 이해하는 것은 예방 전략을 개발하는 것이 필요하다. 예방 전략으로 구명조끼 및 구명기 설치 의무화, 안전교육 활성화, 수영 능력 향상, 인명구조인력 배치 증가 등이 있다.

• 대한본초학회지
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• 제30권5호
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• pp.29-43
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• 2015

Objectives : This study was aimed to analyze the correlation between the Herbology and contemporary research results, KCD-codes.Methods : Papers were searched in OASIS and PubMed, then they were categorized. Medicine or pharmacy articles about Menthae Herba were matched with the Herbology treatment and KCD-codes. Other articles were analyzed by abstract of the papers. KCD-codes and terms were arranged by the Herbology treatment. The Degree of Herbology research (HDR) was calculated by numbers of papers, study method, citation rates.Results : There were 97 articles about Menthae Herba. Among these there were 47 medicine and pharmacy articles about Menthae Herba, and 15 articles were matched to the Herbology treatment. Studies about Headache and Wind-warmth was more active than others. Analysis of other articles showed that studies about contraceptive and anti-oxidative effect, plant growth, protective effect from insect, component extraction technics were active, too. In HDR, headache was scored by 136, Wind-warmth by 104, Eye hemorrhage by 51, Discomport in the throat by 50, Distention and fullness in the chest and hypochondrium by 15, Rubella and Measles by 0.Conclusions : 97 articles about Menthae Herba were analyzed and 15 articles were matched to the Herbology treatment. Studies about headache, wind-warmth, eye-hemorrhage were more active than others of the Herbology treatment. And studies about contraceptive and anti-oxidative effect, plant growth, protective effect from insect, component extraction technics could be a new subject of the Herbology.

• Biomolecules & Therapeutics
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• 제23권6호
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• pp.525-530
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• 2015

Ceramide is the most abundant lipid in the epidermis and plays a critical role in maintaining epidermal barrier function. Overall ceramide content in keratinocyte increases in parallel with differentiation, which is initiated by supplementation of calcium and/or vitamin C. However, the role of metabolic enzymes responsible for ceramide generation in response to vitamin C is still unclear. Here, we investigated whether vitamin C alters epidermal ceramide content by regulating the expression and/or activity of its metabolic enzymes. When human keratinocytes were grown in 1.2 mM calcium with vitamin C ($50{\mu}g/ml$) for 11 days, bulk ceramide content significantly increased in conjunction with terminal differentiation of keratinocytes as compared to vehicle controls (1.2 mM calcium alone). Synthesis of the ceramide fractions was enhanced by increased de novo ceramide synthesis pathway via serine palmitoyltransferase and ceramide synthase activations. Moreover, sphingosine-1-phosphate (S1P) hydrolysis pathway by action of S1P phosphatase was also stimulated by vitamin C supplementation, contributing, in part, to enhanced ceramide production. However, activity of sphingomyelinase, a hydrolase enzyme that converts sphingomyelin to ceramide, remained unaltered. Taken together, we demonstrate that vitamin C stimulates ceramide production in keratinocytes by modulating ceramide metabolicrelated enzymes, and as a result, could improve overall epidermal barrier function.

• 생약학회지
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• 제40권4호
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• pp.345-350
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• 2009

Activity-guided fractionation of the EtOH extract of flowers of Bidens bipinnata L. have been furnished five flavonoids, sulfuretin(1), butein(2), 7,8,3',4'-tetrahydroxyflavanone(3), maritimetin(4) and okanin(5). All of the compounds showed significant activities on both linoleic acid peroxidation inhibition and DPPH radical scavenging effect. The evaluation for protective effect of isolated compounds against tacrine-induced cytotoxicity in human liver-derived Hep G2 cells was conducted. Compounds 1, 3, 4 and 5 showed significant protective effects with the $EC_{50}$ values of $36.1{\pm}0.9$, $23.3{\pm}0.7$, $41.0{\pm}1.0$ and $29.8{\pm}1.1{\mu}M$, respectively. Silybin, one of the well-known hepatoprotective agents, used as a positive control, and also showed protective effect with an $EC_{50}$ value of $84.3{\pm}0.7{\mu}M$.