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Antioxidative and Hepatoprotective Effect of Compounds from the Flowers of Bidens bipinnata L.  

Kwon, Ji-Wung (College of Life Science and Natural Resources, Wonkwang University)
Byun, Erisa (College of Pharmacy, Wonkwang University)
Lee, Eoh-Jin (College of Life Science and Natural Resources, Wonkwang University)
Kim, Youn-Chul (College of Pharmacy, Wonkwang University)
Jeong, Gil-Saeng (Zoonosis Research Center, Wonkwang University)
An, Ren-Bo (Key Laboratory of Natural Resources of Changbai Mountain & Funcional Molecules (Yanbian University) Ministry of Education)
Kwon, Tae-Oh (College of Life Science and Natural Resources, Wonkwang University)
Publication Information
Korean Journal of Pharmacognosy / v.40, no.4, 2009 , pp. 345-350 More about this Journal
Abstract
Activity-guided fractionation of the EtOH extract of flowers of Bidens bipinnata L. have been furnished five flavonoids, sulfuretin(1), butein(2), 7,8,3',4'-tetrahydroxyflavanone(3), maritimetin(4) and okanin(5). All of the compounds showed significant activities on both linoleic acid peroxidation inhibition and DPPH radical scavenging effect. The evaluation for protective effect of isolated compounds against tacrine-induced cytotoxicity in human liver-derived Hep G2 cells was conducted. Compounds 1, 3, 4 and 5 showed significant protective effects with the $EC_{50}$ values of $36.1{\pm}0.9$, $23.3{\pm}0.7$, $41.0{\pm}1.0$ and $29.8{\pm}1.1{\mu}M$, respectively. Silybin, one of the well-known hepatoprotective agents, used as a positive control, and also showed protective effect with an $EC_{50}$ value of $84.3{\pm}0.7{\mu}M$.
Keywords
Bidens bipinnata L.; antioxidative; hepatoprotective; Hep G2 cells; tacrine;
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