• Proceedings of the PSK Conference
• /
• 2002.04a
• /
• pp.189.1-189.1
• /
• 2002

• BMB Reports
• /
• v.55 no.12
• /
• pp.609-614
• /
• 2022

Mutation of the gene for adenomatous polyposis coli (APC), as seen in Apc^Min/+ mice, leads to intestinal adenomas and carcinomas via stabilization of β-catenin. Transmembrane 4 L six family member 5 (TM4SF5) is involved in the development of non-alcoholic fatty liver disease, fibrosis, and cancer. However, the functional linkage between TM4SF5 and APC or β-catenin has not been investigated for pathological outcomes. After interbreeding Apc^Min/+ with TM4SF5-overexpressing transgenic (Tg^TM4SF5) mice, we explored pathological outcomes in the intestines and livers of the offspring. The intestines of 26-week-old dual-transgenic mice (Apc^Min/+:Tg^TM4SF5) had intramucosal adenocarcinomas beyond the single-crypt adenomas in Apc^Min/+ mice. Additional TM4SF5 overexpression increased the stabilization of β-catenin via reduced glycogen synthase kinase 3β (GSK3β) phosphorylation on Ser9. Additionally, the livers of the dualtransgenic mice showed distinct sinusoidal dilatation and features of hepatic portal hypertension associated with fibrosis, more than did the relatively normal livers in Apc^Min/+ mice. Interestingly, TM4SF5 overexpression in the liver was positively linked to increased GSK3β phosphorylation (opposite to that seen in the colon), β-catenin level, and extracellular matrix (ECM) protein expression, indicating fibrotic phenotypes. Consistent with these results, 78-week-old Tg^TM4SF5 mice similarly had sinusoidal dilatation, immune cell infiltration, and fibrosis. Altogether, systemic overexpression of TM4SF5 aggravates pathological abnormalities in both the colon and the liver.

• Advances in Traditional Medicine
• /
• v.10 no.4
• /
• pp.310-318
• /
• 2010

Verbena officinalis (Verbenaceae) has been used as herbal medicine or health supplement in both Western and Eastern countries for centuries. In the present study, we examined the anti-inflammatory and antioxidant activities of the methylene chloride fraction of V. officinalis (VMC). To elucidate the anti-inflammatory properties of VMC, we investigated the inhibition effects of nitric oxide production in interferon-gamma (IFN-$\gamma$) and lipopolysaccharide-stimulated mouse peritoneal macrophages. VMC suppressed nitric oxide production, inducible nitric oxide synthase and cyclooxygenase-2 expression dose-dependently without notable cytotoxicity. In various radical scavenging assays, VMC exhibited strong scavenging effect on DPPH radical, superoxide radical, nitric oxide radical and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical. VMC also showed potent reducing power. These findings strongly suggest that VMC may be beneficial in oxidative stress-mediated inflammatory disorders.

• Advances in Traditional Medicine
• /
• v.2 no.1
• /
• pp.47-51
• /
• 2002

There are over 4,000 different chemicals in cigarette smoke, including nicotine and tar. These compounds influence on lung tissue directly or indirectly. In this study, we have examined whether an aqueous extract of Se-Yeon-Eum (SYE), composed of Oriental medicine that has been known to be effective to symptom by smoking, inhibits nicotine- or cigarette smoke extract (CSE)-induced cytotoxicity in human embryonic lung fibroblast, MRC-9. Assessment of cell viability using 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay indicated that SYE inhibited not only nicotine-induced cytotoxicity but also CSE-induced cytotoxicity. These results suggest the possibility that the use of SYE may be useful for improvement of many symptoms by smoking.

• Advances in Traditional Medicine
• /
• v.10 no.2
• /
• pp.51-58
• /
• 2010

Vitex rotundifolia (V. rotundifolia) has been used for treating headache, dizziness, toothache and removal of fever as a traditional medicine in Korea. In the present study, we examined the antioxidant and anti-inflammatory activities of 85% methanol extract of V. rotundifolia. In various radical scavenging assays, V. rotundifolia exhibited strong scavenging effect on 1, 1-diphenyl-2-picrylhydrazyl free radical, superoxide radical, nitric oxide. To elucidate the anti-inflammatory properties of V. rotundifolia, we investigated the inhibition effects of nitric oxide production in IFN-gamma and LPS-stimulated mouse peritoneal macrophages. V. rotundifolia suppressed nitric oxide production, iNOS and COX-2 expression dose-dependently through suppression of NF-$\hat{e}B$ activation without notable cytotoxicity. These findings mean that V. rotundifolia may be beneficial in oxidative stress-mediated inflammatory disorders.

• Korean Journal of Clinical Pharmacy
• /
• v.10 no.1
• /
• pp.42-49
• /
• 2000

The undergraduate curricula of the twenty pharmacy schools in Korea were analyzed in order to find out the relationship between the Korean Pharmacist Licensure Examination (KPLE) and the Pharmacy Education. The results of this survey indicated that the Pharmacy Education was highly related to KPLE. During the 1999 academic year, there were 1,853 undergraduate courses offered by the twenty pharmacy colleges excluding pre-pharmacy courses. Of the total courses 73\%$ were related to the twelve subject of KPLE. Eighty percent were related to the basic pharmacy. Only $13\%$ were related to the pharmacy practice. The courses related to the Medicinal Chemistry were 16\% of the basic pharmacy courses. The numbers and the names of the subjects of KPLE have not been change during the past 3- years. Recently, the names of the subjects of KPLE have not been changed during the past 30 years. Recently, the National Health Personnel Licensing Examination Board advised to change the courses from the drug-oriented curriculum to a practice-oriented one. Therefore, it will be better that the curriculum of the undergraduate pharmacy program to be re-organized focusing on the divisions of the courses, not on the name of the courses. The number of the under graduate courses should be reduced by combining the related courses. The more courses in the field of patient-oriented pharmacy practice are needed to be added to the undergraduate curriculum in order to produce the clinical pharmacists.

• Bulletin of the Korean Chemical Society
• /
• v.30 no.12
• /
• pp.2881-2882
• /
• 2009

• Korea Journal of Hospital Management
• /
• v.6 no.1
• /
• pp.62-84
• /
• 2001

The purpose of the study was to compare patient's satisfaction on the pharmacy services after introducing the new system of separation of dispensing from prescribing medicines, between those filling their prescriptions from the pharmacy nearby hospitals and those from the pharmacy in their resident areas. To measure patient's satisfaction, a questionnaire survey was conducted with 354 outpatients who received prescriptions from any of the three university hospitals located in In-Cheon city. Study results showed that geographic accessibility to pharmacy was a main attribute to select pharmacy. Size of the pharmacy and availability of prescription drugs are the second major reasons for pharmacy selection for the patients from the pharmacy nearby hospital, whereas patronage is the second major reason for those from the pharmacy in resident areas. Overall satisfaction was higher among the patients from the pharmacy in resident areas than those from the pharmacy nearby hospitals, mainly due to better facilities(waiting area, public telephone, etc), kindness, cleanliness, shorter waiting time, and pharmacist's concern about patient's health. On the other hand, the patients of the pharmacy nearby hospitals showed low satisfaction because of long waiting time and the lack of pharmacist's knowledge and information about patients' health status and medication history. Patients visiting the hospital that has pharmacy-hospital cooperation system showed higher satisfaction as compared to those visiting the hospital without such system. This study provided an empirical evidence that it would be more advantageous for patients to receive pharmacy services from pharmacies located in their living areas than from pharmacies nearby hospitals. This implies that there is a strong need for adequate strategies to enhance the role of pharmacies in resident areas under the new system of separation of dispensing from prescribing medications.

• YAKHAK HOEJI
• /
• v.58 no.3
• /
• pp.208-222
• /
• 2014

Pharmacy practice experiences play a central role in improving the professional abilities and roles of student pharmacists in the changed '2+4'-year curricula of Korean pharmacy schools. For the first 3 years, the students usually learn theoretical areas, and for the last year, they apply learned knowledge to actual pharmacy practice environments. Especially, in order for community pharmacy practice experiences to become firmly established, it is important to evaluate students' feedbacks of community pharmacy practice experiences. However, there have been little studies regarding this area in Korea. This study was to analyze pharmacy students' perceptions and evaluations after community pharmacy practice experiences. The self-reported questionnaire using a 5-point Likert scale was utilized to collect data from the pharmacy students completing community pharmacy practice experiences. Total 62 students responded to the survey questionnaires. The average of students' evaluations of preceptors for overall evaluation items was 4.31 whereas that of student evaluations of practice sites for them was 4.03. The self-evaluations of students' expertises were mostly positive except for that of populations care. The evaluation item 'I believe this experience will help me be a better pharmacist' received the highest satisfaction. According to the results regarding open-ended questions, the students learned the needs for academic motivations and self-developments, and they thought that the curriculum should be standardized and systematized in order to improve contents for community pharmacy practice experiences. Furthermore, based on these results, the guidelines of community pharmacy practice experiences should be modified, and researches on the validation and evaluations of the modified guidelines should be implemented.

• 한국임상약학회:학술대회논문집
• /
• 2007.12a
• /
• pp.193-193
• /
• 2007