• Journal of Animal Science and Technology
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• 제64권5호
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• pp.937-949
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• 2022

Health concern of dogs is the most important issue for pet owners. People who have companied the dogs long-term provide the utmost cares for their well-being and healthy life. Recently, it was revealed that the population and types of gut microbiota affect the metabolism and immunity of the host. However, there is little information on the gut microbiome of dogs. Hericium erinaceus (H. erinaceus; HE) is one of the well-known medicinal mushrooms and has multiple bioactive components including polyphenol, β-glucan, polysaccharides, ergothioneine, hericerin, erinacines, etc. Here we tested a pet food that contained H. erinaceus for improvement in the gut microbiota environment of aged dogs. A total of 18 dogs, each 11 years old, were utilized. For sixteen weeks, the dogs were fed with 0.4 g of H. erinaceus (HE-L), or 0.8 g (HE-H), or without H. erinaceus (CON) per body weight (kg) with daily diets (n = 6 per group). Taxonomic analysis was performed using metagenomics to investigate the difference in the gut microbiome. Resulting from principal coordinates analysis (PCoA) to confirm the distance difference between the groups, there was a significant difference between HE-H and CON due to weighted Unique fraction metric (Unifrac) distance (p = 0.047), but HE-L did not have a statistical difference compared to that of CON. Additionally, the result of Linear discriminate analysis of effect size (LEfSe) showed that phylum Bacteroidetes in HE-H and its order Bacteroidales increased, compared to that of CON, Additionally, phylum Firmicutes in HE-H, and its genera (Streptococcus, Tyzzerella) were reduced. Furthermore, at the family level, Campylobacteraceae and its genus Campylobacter in HE-H was decreased compared to that of CON. Summarily, our data demonstrated that the intake of H. erinaceus can regulate the gut microbial community in aged dogs, and an adequate supply of HE on pet diets would possibly improve immunity and anti-obesity on gut-microbiota in dogs.

• 한국동물위생학회지
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• 제24권2호
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• pp.109-116
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• 2001

This study was conducted to investigate the prevalence rate for rabies antibody (PRRA) of dogs near the Pukhansan National Park and in some other districts in Seoul Metropolitan city. From April to July 2000, a total of 414 serum samples were taken from dogs for breeding (92), pet dogs (162), and unclaimed/stray dogs (162). Rabies virus antibodies were detected by neutralizing peroxidase-linked assay (NPLA). Of 414 sera tested, 145 (35%) were positive to rabies virus antibody. PRRA in Pukhansan National Park area and in the other districts of Seoul city were 34.8% and 35.4%, respectively. There was no significant difference in the prevalence rate between these two districts. PRRA in pet dogs, unclaimed/stray dogs, and dogs for breeding were 39.5%, 35%, and 27.2% respectively. PRRA in dogs from residential areas, apartments, animal hospitals, and farms were 32.5%, 60%, 35.3%, and 26.7% respectively. Especially, the dogs reared in apartments had a significantly higher seroprevalence (60%) than those in residential or farm areas. PRRA in less than 1 year, 1~<2 years, 2~<3 years, and over 3 years old dogs were 14.7%, 40.4%, 38.4%, and 53% respectively, and overall 35% in the dog population. In addition, we found that dogs less than 1 year of age had lower antibody prevalence than those over 1 year of age. It was concluded that enhancement of vaccination is important in the prevention of the rabies, and that rabies vaccines should not be less supplied than the population of the dog.

• Parasites, Hosts and Diseases
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• 제53권1호
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• pp.125-128
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• 2015

The prevalence and genotype of Toxoplasma gondii infection in dogs in Henan Province, Central China was investigated. A total of 125 blood samples were collected from pet dogs during April to June 2013, and all samples were examined by indirect hemagglutination antibody test (IHA) and nested PCR. The overall T. gondii prevalence in pet dogs was 24.0% (30/125), with 20.8% (26/125) in IHA and 10.4% (13/125) in PCR, respectively. No statistical associations were found between animal gender and age and the prevalence of T. gondii infection. Thirteen positive DNA samples were genotyped using 11 PCR-RFLP markers, including SAG1, (3'+5') SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico. Of these, only 2 samples were genotyped with complete data for all loci, and a novel genotype (type III at SAG3 and GRA6 loci, and type I at other loci) was identified. This is the first report of genetic characterization of T. gondii infection in dogs in China.

• Parasites, Hosts and Diseases
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• 제34권2호
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• pp.143-150
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• 1996

애완견에서 진드기에 의한 피부병의 빈토 및 원인 진드기를 조사하고 인체의 소양성 피부 질환의 원인으로 애완견 진드기의 의의를 조사하기 위하여 1990년 1월부터 1995년 12월까지 청운동물병원에서 귀 부위에 피부병이 있었던 970마리의 애완견의 털과 인설에서 진드기를 수세법으로 검출하고 분류하였으며 가족의 소양성 피부질환 유무를 조사하였다. 애완견 피부염의 원인으로 추정되는 진드기로는 Qctodectes Wnotis Hering. 1,838 (귀진드기)와 Sarcoptes canis Gerlach. 1,857 (개옴진드기)가 검출되었으며 귀진드기는 970마리의 애완견 중 382마리 (39.4%)에서 총 2 117마리가 검출되었다. 개옴진드기는 970마리의 애완견 중 3마리 (0.3%)에서 총 30마리가 검출되어 원인 진드기가 검출된 애완견은 970마리 중 385마리 (39. 7%)이었다. 귀진드기가 검출된 382마리의 애완견 사육 가족 중 2가족에서 소양성 피부질환이 발생하였으나 귀진드기와는 관련이 없었으며 개옴진드기가 검출된 3마리의 애완견 사육 가족 중 2가족에서 개옴진드기에 의한 것으로 추정되는 피부염이 발생하였다. 그러므로 본 저자들은 귀진드기가 애완견에서 가장 흔히 검출되는 진드기이지만 사람에게 전파되어 피부염을 유발하지 않으며 개옴진드기는 감염률은 매우 낮지만 사람에 전염성이 강함을 확인하였다.

• 한국동물위생학회지
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• 제25권1호
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• pp.1-7
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• 2002

Recently, the rabies cases have been reported in Paju- and Yangju-gun, Gyeonggj province near Seoul metropolitan area. The Pukhansan national park, nearly located from the cities, is suspected to be a high risk area for incidence and spread of the rabies to metropolitan area. This study was conducted to investigate the prevalence rate for rabies antibody of dogs near the Pukhansan national park and in some other districts in Seoul metropolitan city. From march to october 2001, a total of 306 serum samples were taken from dogs for breeding(189) md pet dogs(117) in 4 districts near the Pukhansan national park and other districts of Seoul. Rabies virus antibodies in sera were detected by neutralizing peroxidase - linked as say (NPLA). Of the 306 sera of dogs tested, 74 (24.2%) were positive to rabies virus antibody. The prevalence rates of rabies antibody in Pukhansan national park area and in the other districts of Seoul city were 23.7% and 25.3%, respectively There was no significant difference in the prevalence rate between these two districts. The prevalence rates of rabies antibody in pet dogs and dogs for breeding were 40.2% and 14.3% respectively. The prevalence rates of rabies antibodies in less than 1 year, 1∼<2 years, 2∼<3 years, and over 3 years old dogs were 14.5%, 22.4%, 32.6%, and 27.1%, respectively, and overall 24.2% in the dog population. In addition, we found that dogs less than 1 year old had lower antibody prevalence than those over 1 year old. It was concluded that enhancement of vaccination is important in the prevention of the rabies, and that rabies vaccines should not be less supplied than the population of the dog.

• Journal of Veterinary Science
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• 제22권3호
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• pp.37.1-37.14
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• 2021

Background: Ticks are one of the most common external parasites in dogs, and are associated with the transmission of a number of major zoonoses, which result in serious harm to human health and even death. Also, the increasing number of pet dogs and pet owners in China has caused concern regarding human tick-borne illnesses. Accordingly, studies are needed to gain a complete understanding of the bacterial composition and diversity of the ticks that parasitize dogs. Objectives: To date, there have been relatively few reports on the analysis of the bacterial community structure and diversity in ticks that parasitize dogs. The objective of this study was to investigate the microbial composition and diversity of parasitic ticks of dogs, and assessed the effect of tick sex and geographical region on the bacterial composition in two tick genera collected from dogs in China. Methods: A total of 178 whole ticks were subjected to a 16S ribosomal RNA (rRNA) next generation sequencing analysis. The Illumina MiSeq platform targeting the V3-V4 region of the 16S rRNA gene was used to characterize the bacterial communities of the collected ticks. Sequence analysis and taxonomic assignment were performed using QIIME 2 and the GreenGene database, respectively. After clustering the sequences into taxonomic units, the sequences were quality-filtered and rarefied. Results: After pooling 24 tick samples, we identified a total of 2,081 operational taxonomic units, which were assigned to 23 phyla and 328 genera, revealing a diverse bacterial community profile. The high, moderate and low prevalent taxa include 46, 101, and 182 genera, respectively. Among them, dominant taxa include environmental bacterial genera, such as Psychrobacter and Burkholderia. Additionally, some known tick-associated endosymbionts were also detected, including Coxiella, Rickettsia, and Ricketssiella. Also, the potentially pathogenic genera Staphylococcus and Pseudomonas were detected in the tick pools. Moreover, our preliminary study found that the differences in microbial communities are more dependent on the sampling location than tick sex in the tick specimens collected from dogs. Conclusions: The findings of this study support the need for future research on the microbial population present in ticks collected from dogs in China.

• 생명과학회지
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• 제30권11호
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• pp.1021-1032
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• 2020

전 세계적으로 반려동물과 반려동물 양육 인구는 증가하고 있으며 이와 관련된 사업은 고급화, 전문화되고 있다. 특히, 개는 사람과 수 천년 동안 공생하고 있으며 이들은 탄수화물 식이와 더불어 사람과 비슷한 생활 방식을 영위하고 있다. 주거와 음식을 공유하며 형성된 강력한 유대관계를 통해 사람은 반려견에 대한 책임감 및 정서적 안정을 느끼며 반려견은 사람을 의지하는 행동을 보인다. 하지만 사람과 반려견 사이에는 식이와 관련된 스트레스가 존재한다. 반려견은 본질적으로 육식성이므로, 반려견에게 영양학적으로 균형 잡힌 식이와 기능성 물질들을 제공하는 것은 그들의 건강을 위한 장내 미생물 군집 유지에 매우 중요하다고 할 수 있다. 장내미생물은 병원균과 같은 외부 유해인자로부터 반려견을 보호하고 면역계를 조절, 생리학적 항상성에도 관여하기 때문에 최근 높은 관심을 받고 있다. 비정상적인 장내미생물 군집 형성은 장질환, 대사질환, 정신질환, 신경계 질환의 병리적 과정과 증상에 영향을 미칠 뿐만 아니라 반려견의 질병은 사람에게 기회감염을 통해 영향을 미칠 수 있다. 따라서 본 논문에서 반려견의 장내미생물의 조성, 다양성뿐만 아니라 장내미생물과 질병 사이의 상관성에 대하여 논의하고자 한다.

• 한국임상수의학회지
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• 제14권2호
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• pp.287-296
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• 1997

Serial ultrasonographic examinations were daily performed on 8 bitches (5 Maltese and 3 Yorkshire terrier) from day 15 until parturition to determine the size of gestational structures. Gestational age was timed from the day of ovulation (day 0), which was estimated to occur when plasma progesterone concentration was first increased above 4.0 ng/ml. When the size of fetal and extra-fetal structures according to gestational age was measured, there was a difference between Maltese and Yorkshire terrier in extra-fetal structures (P<0.001), and no difference in fetal structrures (P<0.001). The correlations between the gestational age and the growth in fetal and extra-fetal structures were examined by regression analysis. The extra-fetal structure which showed high correlation from day 20 to 35 was inner chorionic cavity diameter in two breeds. The fetal structures which showed high correlation from day 36 until parturition was fetal head diameter in two breeds. In conclusions inner chorionic cavity diameter was the most accurate for estimation of gestational age and prediction of whelping day from day 20 to 35, and fetal head diameter from day 36 to parturitions respectively. Ultrasonography is very useful tool to evaluate the gestational age and to predict the whelping day in pet dogs.

• Journal of Information Technology Applications and Management
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• 제26권6호
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• pp.79-87
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• 2019

Blockchain is a distributed network in a decentralized P2P infrastructure. In this paper, we propose and implement a blockchain-based pet trade service DApp(Decentralized Application). In the existing trading services, there is no way to know the status of dogs in interpersonal transactions and an additional distribution cost has been incurred. As a solution to this problem, we introduce a blockchain technology for a pet trade service where users can check whether an individual pet is healthy or not by its registered biometric data in order to have an efficient and confident trade service. The registered users can purchase puppies or registering information for their dogs to sell through a pet trade service DApp. It is also possible to make a reliable trade by verifying whether the dog is actually the same one through an inscription information of a dog.

• 한국융합학회논문지
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• 제10권11호
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• pp.159-164
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• 2019

ICT 기술 발전으로 강아지와 고양이등 반려동물 돌보기와 건강에 대한 관심도가 높아지고 있다. 본 논문에서는 반려동물 산업의 다양한 분야에 활용될 수 있도록 반려동물 사료의 성분 데이터를 기반으로 군집분석을 수행하고 적합한 서비스에 대해 고찰한다. 군집분석을 위해 시중에서 유통되고 있는 300여 개의 강아지 및 고양이 펫푸드를 대상으로 성분별 상관관계를 분석하여 유사성을 측정하며, Hierarchical, K-Means, Partitioning around medoids(PAM), Density-based, Mean-Shift 등의 다양한 클러스터링 기법을 활용하여 군집화 하여 분석한다. 또한 반려동물의 개인화 추천시스템도 제안한다. 본 논문의 연구 결과는 반려동물을 대상으로 한 사료 추천시스템 등의 맞춤형 개인화 서비스에 활용할 수 있다.