• Korean Journal of Agricultural Science
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• v.40 no.2
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• pp.123-129
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• 2013

An experiment was conducted to evaluate the effects of nitrogen supplementation through urea-molasses multinutrient block (UMMB) on the performance of dairy cows fed good quality forage based diets with rice straw as a night feeding. A total of 10 multiparous crossbred dairy cows in their early lactation were grouped into two categories based on their breed, parity, body weight, milk yield, milk fat and protein contents and daily fed a chopped CO-3 grass (Pennisetum purpureum ${\times}$ Pennisetum americanum; hybrid Napier) ad-libitum, 1 kg of dairy cow concentrate feed during the day time and 5 kg of rice straw (dry matter basis) at night as the basal diet (control) for 5 wk. In addition to the basal diet, the treatment group received 300 g of crushed UMMB daily throughout the experimental period. Cows were milked twice daily and the milk yields were recorded. Milk and feed samples were collected weekly for chemical analysis. Supplementation of UMMB had no significant effects (p>0.05) on straw intake, daily milk yield, contents and yields of milk constituents such as milk fat, protein, lactose and solids-non-fat. In addition, milk urea nitrogen content were not affected (p>0.05) by UMMB supplementation. However, numerical increments in all the parameters measured were observed during the study in cows fed diets supplemented with UMMB. It can be concluded that nitrogen supplied through UMMB had no effects on production performances of dairy cows in this study.

• Journal of the Korean Society of Food Culture
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• v.24 no.6
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• pp.588-596
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• 2009

To determine the relationships between soybean food intake, dietary isoflavone intake, and osteoporosis incidence, questionnaire surveys, bone mineral density measurements, and dietary surveys by food record were performed with 19 postmenopausal women ($57.6{\pm}7.3$ yrs of age) in Daejeon city. The subjects were divided into two groups: an 'osteoporosis group' (OG, n=10) and a 'normal group' (NG, n=9). Mean age, height, and body weight were similar between the two groups but BMI was higher in OG than in NG. Mean age at menarche was not different between the two groups. However, mean number of childbirths was greater in OG than in NG and mean total period of lactation was shorter in OG. Mean exercise time per week was similar between the two groups, and mean time of sunlight exposure tended to be shorter in OG. Mean daily intake of calcium was lower in OG whereas sodium intake was higher in OG. Mean daily intakes of total soybean food (OG: $203.8{\pm}84.6\;g/d$, NG: $285.0{\pm}146.3\;g/d$) and total dietary isoflavone (OG: $29.1{\pm}14.3\;mg/d$, NG: $38.3{\pm}23.1\;mg/d$) were not different between the two groups. However, greater intakes of soybean food and dietary isoflavone were associated with higher bone mineral density, respectively. The above results indicate that osteoporosis incidence tends to be influenced by BMI, maternal factors, dietary nutrient intakes, soybean food intakes, and dietary isoflavone intakes in postmenopausal women; although no significant differences in soybean food and dietary isoflavone intake were found between the two groups. This tendency implies that greater intakes of soybean food and dietary isoflavone lead to lower incidence of osteoporosis in postmenopausal women.

• Journal of Embryo Transfer
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• v.1 no.1
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• pp.35-49
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• 1986

Recipients are an integral part of embryo transfer and they are expensive to maintain as a good recipient. Recipient management is one of the most important components in a successful embryo transfer program. Management includes selection and subsequent care of the animals. A good recipient is basically on "open" cows or heffers whose reproductive tract is capable of receiving one or two embryos and incubating it to term. Potential recipients should be always be healthy and cycling normally ranging from 18 to 23 days. A thorough veterinary examination is recommended for candidate of recipients and cattle for questionable health should be eliminated from the recipient herd. Age and size of recipients are particularly important considerations when heifers are used, because of most embryos available for transfer are from large dams and sires. Body condition can influence a recipient's production, reproduction and health. Obese and underconditioned cattle should be avoided for use. Transfer of fresh embryos especially requires precise synchronization of donors and recipients. For estrus synchronization, PGF$_2$$\alpha$ is injected twice 10 to 12 days apart and short4erm progestagen treatment is applied to potential recipient cattle by coil into vagina (PRID) or ear implant (Synchro-Mate-B). The highest pregnancy results are achieved in recipients at exact synchrony with donors or 12 to 24 hr earlier than donors. Estrus detection is a major factor in breeding efficiency. High accuracy can be achieved by use of heat mount detection alds or by obserbing cattle for 30-minute peroids 3 times daily. Assay progesterone in milk can be used to discrIminate between pregnant and nonprenant recipients. Rectal palpation on day 35 to 70 after is an accurate and safe method of pregnancy diagnosis. Embryonic mortality in recipients may be associated with factors such as high environmental temperature and nutritional or lactational stress in early lactation period. Achievement of short calving interval requires concentrated management activity during the first 90 days following calving. Acceptable candidate for a recipient should be routinely vaccinated for infectious diseases. Proper nutritional programs according to NRC requirements and body condition scoring system for recipient cattles are vital to the ultimate success of an embryo transfer program.r program.

• Journal of Nutrition and Health
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• v.40 no.2
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• pp.130-137
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• 2007

Myelin basic protein (MBP), a major structural protein of the myelin, is thought to be important for the maintenance of myelin in the central nervous system (CNS). We investigated the effect of maternal folic acid nutritional status on the folate level and the synthesis of MBP in the offspring. In order to test this hypothesis, female Sprague-Dawley rats were fed either folic acid sufficient (8 mg/kg diet) or deficient (0 mg/kg diet) diet from 2 wks prior to the mating throughout the entire pregnancy, lactation and weaning period. We examined plasma folate level by the radioimmunoassay and homocysteine level by HPLC, respectively. The MBP expression was measured by the western blot analysis. The maternal folic acid deficiency decreased plasma folate level with a concomitant increase in plasma homocysteine level in their offspring. The maternal folic acid deficiency decreased hepatic levels of SAM and SAM/SAH ratio with a concomitant increase in hepatic levels of SAH and the MBP expression of spinal cord in their offspring at 7 wks of age. These results suggest that maternal folic acid nutritional status affect plasma folate and homocysteine level in their offspring. Moreover, the maternal folic acid deficiency mi호t inhibit the MBP expression of the spinal cord and disrupt many other vital CNS reactions in their offspring.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.7
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• pp.1138-1147
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• 2020

Objective: A study was made investigate factors affecting body surface temperature changes after weaning in sows, whether these can be used to aid detection of natural estrus and how they relate to subsequent reproductive performance. Methods: A total of 132 sows were selected during summer from a breeding farm, with mean parity of 3.6±2.3 and 28.5±0.9 days lactation length. Four daily measurements (6:00, 8:00, 16:00, and 18:00) of vulva (VST), udder (UST), ear base and central back skin temperatures for individual sows were taken by an infrared thermometer, continuing up to 8 days post weaning. Results: The VST obtained from sows showing estrus at 4 days post-weaning (4DPW), 5DPW, and 6DPW showed a peak at the fourth day post-weaning, but then started to decrease. The VST of sows not detected in standing heat (NDPW) remained at a lower level during the experiment, but UST was increased soon after weaning. The VST-UST temperature differences during daytime of sows that were showing behavioural standing heat on 4DPW, 5DPW, 6DPW, and 7DPW were 0.46℃±0.123℃, 0.71℃±0.124℃, 0.66℃ ±0.171℃, and 0.58℃±0.223℃, respectively. The NDPW sows had the highest UST observed, but also the lowest VST so that a more negative value of temperature difference (-0.31℃) was seen during first few days post-weaning. A total of 119 sows were observed to show standing heat and were bred. The later the estrus, the smaller the litter size (p = 0.005). Conclusion: Sows which did not show behavior indicative of stable standing heat after weaning had a VST which remained at a lower level, but the UST increased soon after weaning. Therefore, for sow heat detection under field conditions, the changes of VST and UST and difference between the two should be considered together to increase the accuracy of detection.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.4
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• pp.333-339
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• 1991

Eighty-two ewes of Balochi breed, two to four years of age were used in a completely randomized design to study the effect of winter supplementation on their performance in Kalat area of Balochistan and randomly divided into two groups of 40 and 42 animals. Two treatments (T1 and T2) studied were : 250 gm/animal/day of a 50 : 50 mixture of cottonseed cake and barley grain fed from Oct. 20 to Dec. 18, 1988 plus grazing and 500 gm/animal/day of the same feed mixture fed from Oct. 9 to Dec. 18, 1988 in addition to grazing. Lucerne hay and wheat straw in a 50 : 50 ratio were provided to all the ewes for a period of one month from Jan. 6, 1989 @ 320 gm/animal/day to sustain them in severe winter. Same feeding levels to the same ewe groups were again fed from Mar. 1 to May 27, 1989. Three breeding rams stayed with the flock from Nov. 1 to Dec. 13, 1988. Lambing took place from Apr. 2 to May 12, 1989. Conception, lambing and mortality percentage was found different (P<.05) between T1 and T2 (12.5 vs 14.8 kg). The ewes on T2 maintained higher body weights throughout winter than the ewes on T1. The results are suggestive of improvement in conception rate with winter supplementation (flushing) and decrease in ewe mortality. Late-gestation and early-lactation supplemental feeding of ewes results in increases in weaning weights of their lambs.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.6
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• pp.821-827
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• 2002

The purpose of this study was to evaluate the effect of protected fat and heat-extruded soybean meal on the lactation performance of Holstein cows. Twenty-four cows, consisting of 20 lactating cows and 4 rumen-fistulated dry cows, were randomly allocated into four groups with 5 lactating cows and 1 fistulated cow in each group. A replicated 4${\times}$4 Latin square design with four 21 day periods, including 14 days of adaptation and 7 collection days within each period was employed. The experiment was a 2${\times}$2 arrangement, with or without heat-extruded soybean meal and protected fat inclusion. The dietary treatments consisted of supplements of (a) soybean meal (the control), (b) heat-extruded soybean meal, (c) protected fat, and (d) heat-extruded soybean meal and protected fat. The results showed that there were no significant differences in feed intake, milk yield, milk protein content, milk lactose content and body weight change between the dietary treatments. However, cows supplemented with protected fat showed a significantly increased (p<0.05) milk fat yield, 3.5% FCM and total solid yield. The increase in undegradable intake protein (UIP) via heat extruded soybean meal supplement significantly decreased the urea nitrogen concentration in the blood (p<0.05). Dietary fat inclusion significantly increased the blood cholesterol concentration (p<0.01) and decreased the ruminal pH value (p<0.01). Increased dietary UIP significantly decreased the ammonia nitrogen concentration in the rumen (p<0.01), but did not significantly influence the pH and VFA molar percentage in the rumen. It appears that dietary protected fat inclusion could improve milk fat and solid content. Increased dietary undegradable intake protein through heat extruded soybean meal did not improve milk yield. But it could alleviate the adverse effect of decreased milk protein due to dietary fat supplementation. Increased UIP could also decrease the ammonia nitrogen concentration in the rumen and plasma urea nitrogen concentration in the blood.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.11
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• pp.1516-1519
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• 2001

The data consisted of 369 lactation records for calvings over a sixteen-year period (1972-1987) and included only cows that had normal milk records. The data were analysed using a linear model containing the fixed effects of parity, year of calving and season of calving. The least squares means${\pm}$S.E. were $1,273{\pm}58.4kg$ for milk yield, and for post-partum body weight (kg) at 2, 3 and 4 months after calving were $343.40{\pm}3.96$, $346.10{\pm}4.10$ and $352.54{\pm}4.26$, respectively. With the exception of season of calving, the effects of parity and year of calving were significant (p<0.01) on the performance of the animals. Thus, the mean-milk yields 1162, 1351 and 1350, were similar for pre-, peak- and post rainy seasons, respectively. On the other hand, as parity increased from 1 to 3, milk yield also increased, but thereafter, there was a gradual decline in milk yield. Similarly, post-partum body weight also increased with parity. However, no consistent pattern for year effect was observed which probably was a reflection of the variation in climatic conditions, or forage quality and/or availability. The phenotypic correlations between milk yield and post-partum body weights were negative and small (ranging from -0.01 to -0.08). However, high milk production in cows was associated with longer calving interval. The implications from the phenotypic correlations are suggestive of one or two possibilities. Firstly, selection for increased body weight may actually result in decreased milk yield. Also, a substantial genetic antagonism may exist between milk yield and fertility in the crossbred cows. Therefore, it is important that selection to improve milk production should take into consideration the reproductive performance of the cows.

• Korean Journal of Community Nutrition
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• v.10 no.1
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• pp.59-69
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• 2005

The purpose of this study was to estimate the mineral intakes and serum mineral levels of pregnant and lactating women. The subjects consisted of 34 non-pregnant, 56 pregnant and 20 lactating women. Nutrients intakes were investigated by the 24-hr recall method, and serum major and trace minerals were analyzed by the ICP-spectrometry. Calcium (Ca) and zinc (Zn) intakes were observed lower than RDA especially for both pregnant and lactating women. Iron (Fe) intake of pregnant women was $85 - 139\%$ RDA through Fe supplementation, and that of lactating women was lower than RDA. Compared with non-pregnant women, the pregnant women had similar Ca intake and higher magnesium (Mg) intake. Comparing with the non-pregnant women, serum Ca level in pregnancy was lower, and that of lactating women was not significantly different. Serum phosphorus and Mg levels were not significantly different among the groups. Serum Fe level of pregnant and lactating women was lower than that of the non-pregnant women. Serum Zn level of pregnant women was lower than those in the lactating and non-pregnant women. Serum copper level decreased as the pregnancy progressed. Serum sodium (Na) level was higher in 2nd- and 3rd trimester and potassium (K) level was higher in 3rd trimester and lactating period than other groups. Na/K ratio was not significantly different among the groups. During all periods, there was no correlation between dietary intakes and serum levels in each minerals. Serum Ca level positively corrleated with serum Mg level, especially in 3rd trimester and lactating women. In general, serum mineral levels in pregnancy were changed compared to the levels in non-pregnancy and restored in lactation to the levels for non-pregnancy.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.12
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• pp.1705-1711
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• 2014

The objective of this study was to evaluate the effect of ruminally protected amino acids (RPAAs) and ruminally protected fat (RPF) supplementation on ruminal fermentation characteristics (in vitro) and milk yield and milk composition (in vivo). Fourteen mid-lactating Holstein dairy cows (mean weight $653{\pm}62.59kg$) were divided into two groups according to mean milk yield and number of days of postpartum. The cows were then fed a basal diet during adaptation (2 wk) and experimental diets during the treatment period (6 wk). Dietary treatments were i) a basal diet (control) and ii) basal diet containing 50 g of RPAAs (lysine and methionine, 3:1 ratio) and 50 g of RPF. In rumen fermentation trail (in vitro), RPAAs and RPF supplementation had no influence on the ruminal pH, dry matter digestibility, total volatile fatty acid production and ammonia-N concentration. In feeding trial (in vivo), milk yield (p<0.001), 4% fat corrected milk (p<0.05), milk fat (p<0.05), milk protein (p<0.001), and milk urea nitrogen (p<0.05) were greater in cows fed RPAAs and RPF than the corresponding values in the control group. With an index against as 0%, the rates of decrease in milk yield and milk protein were lower in RPAAs and RPF treated diet than those of basal diet group (p<0.05). In conclusion, diet supplemented with RPAAs and RPF can improve milk yield and milk composition without negatively affecting ruminal functions in Holstein dairy cows at mid-lactating.